Objective To construct 7 shRNA plasmids targeted to Gcn5 and to explore their role on histone acetylation in regulating MSCs differentiation into cardiomyocytes.Methods MSCs were isolated and passaged.Seven shRNA plasmids were constructed by pGenesil-1(4.9 kb) vector with GFP gene.MSCs were treated with 10 ?mol/L 5-azacytidine respectively for 24 h,2,4 and 7 d and transfected with the plasmids via lipofectamine~(TM)2000.Gcn5,as the marker of the acetylation,and GATA4,as the marker of cardiomyocytes,were detected in MSCs during differentiation with or without shRNA plasmids transfection by RT-PCR.Results MSCs were fibroblast-like but changed morphologically after induction.Seven Gcn5 shRNA plasmids were constructed and identified by restriction endonucleases PstⅠ/SalⅠand DNA sequencing.The expression of Gcn5 and GATA4 could be detected in MSCs without shRNA plasmids tranfection,but no expression after shRNA plasmids transfection.Conclusion Gcn5 plays a very important role in MSCs differentiation into cardiomyocytes,which lays the basis for further research of the importance of acetylation in the regulation of cell differentiation.

It is a mature technology producing single-use syringes by polypropylene(PP). But the traditional sterilization process for single-use syringe pollutes the environment, and the residues endanger the health of users. Irradiation sterilization, instead of traditional sterilization process, is the developing trend. However, the traditional PP does not have the ability of radiation resistance. These methods can improve the radiation resistance ability of PP effectively by adding the stabilizer or floating agent. Toughening or temperature treatment can have the same function. Radiation-resistant PP is introduced in this paper. Furthermore, the developing trends for the materials are discussed.

? AIM: To investigate the immunosuppression of artemisinin in the early-phase response( EPR) of allergic conjunctivitis in a murine model induced by pollen.?METHODS:Twenty-five Balb/c mice were divided into five groups at random: A: model group, B: artemisinin topical treatment group, C: oral artemisinin group, D:dexamethasone topical treatment group, E: negative control group. Balb/c mice were first sensitized with mixture of ragweed crude extract and complete Freund’s adjuvant by left footpad and root of tails injection at days 0. At days 7 and 14, mice were strengthen sensitized by intraperitoneal injection. Negative control group replacd with a mixture of equal amounts of Freund’s adjuvant and PBS mixture. Treatment time was between 21-27d:group B received 1% topical artemisinin eye drops 4 times per day; group C received artemisinin at doses of 600mg/kg orally once per day; group D received topical dexamethasone eye drops 4 times per day. The model and naive groups replaced with solvent for control. After treatment, mice were excited by ragweed pollen crude infusion drops. Within 1h after the excitation, the eyes were taken for histopathology testing, the serum for detection of specific IgE for ragweed pollen.?RESULTS:After excitation, allergy symptoms of model group were more significantly compared with negative control group and the treatment groups; allergy symptom score, mast cell degranulation ratios and the ragweed pollen specific IgE in serum in the topical treatment groups and oral group were higher than that in the negative control group, but significantly lower than that in model group.?CONCLUSION: Artemisinin topical treatment and oral treatment for mice could inhibit the symptoms of pollen allergic conjunctivitis, early-phase response of mast cell degranulation ratio and specific IgE generation for ragweed pollen, suggesting that artemisinin has some therapeutic effect for pollen allergic conjunctivitis.

Objective To establish the Valproate (VPA) exposure-induced autism spectrum disorder (ASD) model at early pregnancy in rats,and to study the changes of social interaction test and neuroimmune system in autism model rats,and discuss the pathogenic mechanism.Methods Twelve-week-old rats were randomly divided into the non-exposed group (n =10) and the VPA-exposed group (n =10).Their babies were respectively just the normal control group (n =10) and the model group (n =10).The autism-like social behavior was evaluated via the social interaction test.The level of interleukin (IL)-1β,IL-4,IL-6,interferon-gamma (IFN-γ),transforming growth factor-beta (TGF-β) in mothers and offspring were detected by using enzyme-linked immunosorbent assay (ELISA).The histopathological damage in brain was observed with immunohistochemical staining.Results The score of social interaction test in the model group [(-163.16 ± 101.92) scores]was lower than that in the normal control group [(132.73 ± 114.63) scores] (t =-6.100,P ＜ 0.05).The levels of IL-1β,IL-4,IL-6,IFN-γ in VPA-exposed group were higher than those in the non-exposed group (t =5.883,6.394,5.655,5.393,all P ＜ 0.05),while the level of TGF-β was lower than that in the non-exposed group (t =-6.726,P ＜ 0.05).The levels of IL-1β,IL-4,IL-6,IFN-γ in the model group were higher than those in the normal control group(t =3.058,3.048,6.670,5.486,all P ＜ 0.05),while the level of TGF-β was lower than that in the normal control group (t =-6.516,P ＜ 0.05).The change trend of the level of cytokines in the serum of ASD model rats was similar to the change trend of the level of cytokines in the serum of VPA exposed rats.The result of social interaction test the approach-avoidance score was inversely correlated with the levels of IL-1β and IL-4 (r =-0.802,-0.781,all P ＜ 0.05).The result of immunohistochemical staining showed the expressions of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (IBA1) in the model group were higher than those in the normal control group.Conclusions The main neuroimmunological pathogenesis mechanism can be explained as follows:at early pregnancy,the maternal cytokines skewing influenced by VPA exposure can make immune activation,induce immune system dysfunction and affect the brain growth and development,which result in autism-like behavior in offspring.

Objective To investigate changes in the level of serum CEA,Ferritin and plasma D-D of the lung cancer patients suffering before and after the chemotherapy,and evaluate its application value in judging the curative effect of chemotherapy on the lung cancer patients.Methods Chose 120 lung cancer patients diagnosed from September 2011 to September 2014, and determined the curative effect in accordance with the standard for evaluating the curative effect of solid tumors set down by WHO.Chose another group of 120 healthy physical examinees as its control group.Tested the contents of serum CEA, Ferritin and plasma D-D of both groups respectively and analyzed the rules of change in the results of the patients before and after chemotherapy and in different chemotherapeutic effect stages.Results The contents of serum CEA,Ferritin and plas-ma D-D of the lung cancer patients were obviously higher than those of such patients after chemotherapy and those of the control group,and the difference was of statistical significance (t=4.123~14.224,P <0.05).The contents of serum CEA, Ferritin and plasma D-D of the patient at complete remission (CR)stage were lower than those at partial remission (PR) stage,stable disease (SD)stage and progress disease (PD)stage,and the difference was of statistical significance (t=4.230~12.336,P <0.05).Conclusion The test on the serum CEA,Ferritin and plasma D-D of the lung cancer patients before and after chemotherapy is of certain clinical value and the change in their content levels can be taken as a reference index for the chemotherapeutic effect of lung cancer patients.

Objective To investigate application value of changes in serum neuron-specific enolase (NSE)in early diagnosis of diabetic peripheral neuropathy (DPN).Methods 160 cases of diabetes were recruited from January 2013 to December 2014, 120 of which were combined with DPN according to the 2009 “DPN Diagnostic & Treatment Guidelines”(the DPN group), and the other 40 were not (the DM group).Patients were further stratified into mild,moderate,and severe DPNs according to the Toronto Clinical Scoring System (TCSS).Another 160 healthy subjects were recruited as control.Serum NSE level was determined by chemiluminescence immunoassay,and was compared among the DPN group,the DM group,and the con-trol group.Serum NSE levels were also compared among DPN patients with different serum glucose levels and different TC-SS grading.Results Serum NSE levels (μg/L)of the DPN group,DM group,and control group were 23.5±3.0,12.4±2.3 and 11.3±1.6 respectively,and the difference between DPN and the other two groups was significant (t=12.312,14.523, P <0.05).DPN patients with serum glucose levels of 3.9~6.1,6.1 ~8.0,and ≥8.0 mmol/L had NSE levels of 22.1 ± 1.6,23.6±1.2 and 25.0±1.5 respectively,and the difference was significant (t =4.021 ~ 7.534,P <0.05 for all).DPN patients with mild,moderate,and severe DPN had NSE levels of 21.6±1.1,23.1±1.5,25.2±1.3,and the difference was significant (t=3.320~5.526,P <0.05 for all).NSE level was positively correlated with TCSS score (r=0.572,P <0.05). Conclusion Serum NSE level is closely correlated with DPN,and can be used as a key indicator for early diagnosis of DPN.

Objective To study the correlations between the leptin levels and the prevalence cervical carcinoma. Methods Blood samples were collected from 79 patients with cervical squamous cell carcinoma, 28 patients with cervical intraepithelial neoplasia and 35 healthy donors. The serum leptin concentrations were measured by enzyme linked immunosorbent assay. Meanwhile, tissue samples were obtained from the patients with cervical cancer and cervical intraepithelial neoplasia, Then the expressions oleptin in these tissues were detected by Western blot. Results The level of serum leptin in the patients with cervical cancer was significantly higher than those both in the patients with cervical epithelial tumor and in the healthy control group. the Western blot results also confirmed that the the expressions of leptin in the tumor tissues were atwas the highest level. Conclusion The elevated levels of leptin in serum and tumor tissues of patients with cervical cancer suggest that leptin may be involved in the occurrence and development of cervical cancer.

The imported schistosomiasis cases in the Chinese literature and reports from the infectious disease monitoring in-formation system from 1979 to 2013 were collected and compiled. Totally 365 cases were reported to be infected with outside schis-tosomiasis, including 239 cases of schistosomiasis haematobia (74.0%) and 71 cases of schistosomiasis mansoni (22.0%), and 42 foreigners (11.5%) and 323 Chinese (88.5%). The infection areas involved 15 countries and regions in Africa. Totally 74.92%of pa-tients worked in the construction or geological prospecting in the wild field. According to the analysis of these cases, we found that the management system of prevention and control of imported schistosomiasis cases was not perfect including the high missing re-port rate, high misdiagnosis rate, and no standard diagnostic criteria. We suggest that all the levels of CDC or health administrative authorities should adopt the following relevant control measures to strengthen the imported schistosomiasis prevention and control in order to reduce the damage to the public health and the risk of the spread of African schistosomiasis in China:1. to establish and perfect the imported schistosomiasis monitoring and control system in China;2. to integrate the dynamic information platform of la-bor export and establish the comprehensive prevention and control management system of infectious diseases;3. to standardize the diagnosis and treatment of oversea imported schistosomiasis; 4. to strengthen the research on the transmission risk of imported schistosomiasis in the territory of China.

Objective To study the antagonism of water-soluble compounds of salvia-salvianolic acid A(SalA),salvianolic acid B(SalB) and catechu aldehyde(CA) to Cr(Ⅵ) induced genetic toxicity in Vicia faba root tip cells.Methods The root tip of Vicia faba were treated with SalA,SalB,CA(5-1000 ?g/ml) and every compound combined with 100 ?g/ml Cr(Ⅵ) respectively,and with distilled water as control group.The micronucleus rate(MNR),the mitotic index(MI) and the chromosome aberration rate(CAR) of Vicia faba root tip cells were observed by micronucleus assay and chromosome aberration assay.Results Compared with control group,treatments of 50,100 ?g/ml SalA,50 ?g/ml SalB,100,500 ?g/ml CA significantly increased MI(P

Objective To establish a method for the determination of hymecromone content and dissolution in Compound Dantong Capsules by HPLC.Methods The content and dissolution of hymecromone was determined by HPLC.The chromatographic conditions were: CAPCELL PAK C18 Column(5 ? m,4.6 mm? 150 mm),methanol-0.01 mol/L phosphate buffer solution(465 ∶ 535) as mobile phase,detection wavelength at 365 nm and the injected volume being 10? L.Dissolution test was performed with 150 mL of water as dissolving medium and with a rotation speed of 100 r? min-1.Results A good linearity of hymecromone was in the range of 0.25 ? g/mL～ 1.75 ? g/mL(r=0.9989),and the average recovery was 99.4 %,RSD=1.60 %(n=5).The dissolutions of 3 batches of samples in 45 minutes were higher than 70 % of the labeled amount,which accorded with the standard.Conclusion This method was simple and accurate,has no interference of other components,and can be used for the quality control and evaluation of Compound Dantong Capsules.