Girdin protein is a kind of actin binding protein,which has the complex molecular structure and takes part in a variety of signaling pathways related with causing cancer.The biological characteristics of Gir-din protein have close association with the proliferation and invasion of malignant tumor.Recent studies have shown that Girdin protein is closely related with occurrence and progress of breast cancer.Girdin protein expres-sion in breast cancer cells can not only promote the progress of breast cancer,but also affect the lymph node me-tastasis and prognosis.Therefore Girdin protein is expected as an independent biomarker to provide a new direc-tion for breast cancer treatment.

Objective:This research is to observe the immunity state of lung cancer by radiotherapy in sync with chemotherapy combined with pingxiao capsule,and to evaluate the effect on the immune function and the existence qualitative of pingxiao capsule in lung cancer.Method:Sixty lung cancer patients were parted into two groups at random,one is simple radiotherapy in sync with chemotherapy,the other is radiotherapy in sync with chemotherapy combined with pingxiao capsule.All the two groups were carried out T-cell subgroup experiment at the beginning and three weeks after the therapy.Results:After therapy CD3,CD4 and CD4/CD8 all indicates significant lower level(P

Electric fields have special biological effects for its heating and non-heating effects. Heating effects are about the technology of radio frequency and microwave thermotherapy while non-heating effects are alternating electric field and pulsed electric field. These electric fields have significant effects for tumor treating.

Diabetic nephropathy ( DN) is one of the complications of diabetes, which is closely related to the pathogenesis of in-flammation. This article summarized the relevant literatures on the correlation between MCP-1 , TNF-α and DN, and the intervention of traditional Chinese medicine with DN. In kidney tissues, oxidative stress and NF-κB-dependent signal pathways induced the increase of monocyte chemoattractant protein-1 (MCP-1) expression, which could induce macrophage accumulation, proteinuria increase, renal fibrosis and renal clearance capacity decrease, and further leading to kidney damage. The clinical and experimental trails showed tumor necrosis factorαmRNA and protein levels in serum, urine and renal tissues were closely related with DN, which could be used as a bio marker to provide clinical guidance. As the therapeutic targets,MCP-1 and TNF-αcould give a novel insight into the clinical treatment of DN. Some traditional Chinese medicines or monomer could ameliorate DN by inhibiting MCP-1 and/or TNF-α. Thus, further verifi-cation or clinical application of those traditional Chinese medicines is worth trying.

BACKGROUND:Because chondrocytes have no regeneration ability, to select suitable seed cels is the primary problem to repair cartilage defects.
OBJECTIVE:To investigate the effect of alogeneicversus heterologous bone marrow mesenchymal stem cels (BMSCs) in repairing laryngeal cartilage defects after chondrogenic induction.
METHODS:BMSCs from human and rabbits were isolated and cultured. Passage 3 cels were cultured in chondrogenic induction medium containing transforming transforming growth factor beta 1 and bone morphogenetic protein, and then were dropped onto a poly(lactic-co-glycolic acid) (PLGA) scaffold. Thirty New Zealand rabbits were randomly assigned into three groups: blank control group, human BMSCs group, rabbit BMSCs group. Animal models of laryngeal cartilage defects were made in the three groups. After modeling, saline-soaked PLGA scaffold, PLAG scaffold with human BMSCs or with rabbit BMSCs were implanted respectively into the rabbits in the normal blank, human BMSCs and rabbit BMSCs groups. The expression of type II colagen in the larynx and its surrounding tissues was detected by immunohistochemistry at 4 and 8 weeks postoperatively.
RESULTS AND CONCLUSION: The animals in each group breathed normaly with no presence of wheezing, and their eating and activity were good. Moreover, there was no purulency or infection in the three groups. At 4 and 8 weeks after operation, the positive rates of type II colagen in the two BMSCs groups were significantly higher than that in the blank control group (P < 0.05). There was no significant difference between two BMSCs groups (P > 0.05). These results show that both alogeneic and heterologous BMSCs have good therapeutic effects on the repair of laryngeal cartilage defects in rabbits.

Objective:To explore the application value of continuous positive airway pressure ( CPAP) in the treatment of obstruc-tive sleep apnea hypopnea syndrome (OSAHS ) complicated with coronary heart disease (CHD).Methods:Totally 50 patients with OSAHS complicated with CHD from June 2012 to June 2014 in our hospital were randomly divided into the control group and CPAP group with 25 ones in each .The patients in the control group were treated with the conventional drug therapy for coronary heart disease , while those in CPAP group were treated with CPAP additionally .After 6-month treatment, PSG index, clinical symptoms, arrhythmia and ischemia electrocardiogram changes of the patients in the two groups were compared .Results:After the 6-month treatment , AHI and oxygen desaturation index in CPAP group were significantly decreased when compared with those before the treatment , and the min-imum value of SaO2 and the average value of SaO 2 were higher than those before the treatment (P<0.01), and the above indices were better than those in the control group (P<0.01).The incidence rate of chest pain and chest tightness , arrhythmia, times of myocardi-al ischemia in a day and time of myocardial ischemia in a day were decreased after the treatment (P<0.01), and the decrease in CPAP group was more notable than that in the control group (P<0.01).Conclusion: CPAP combined with the conventional drug therapy for coronary heart disease can improve the oxygen concentration and reduce the incidence of arrhythmia and myocardial ischemia in the patients with OSAHS with CHD , which can be widely used in clinics .

Objective: To detect the expression profile of NDRG1 gene in different tissues and cell lines and explore the relationship of NDRG 1 with liver tissue differentiation and hepatocarcinogenesis. Methods: The expression profiles of NDRG 1 in hepatocellular carcinoma (HCC) tissues, paired noncancerous liver (PNL) tissues, adult normal liver (NL) tissues, baby mouse liver tissues and fetal liver tissues in different developmental stages and cultured cell lines were performed using RT PCR and Northern Blot analysis. Results: Expression of NDRG 1 was significantly up regulated in HCC tissues compared to that of NL tissues and PNL tissues, however, the expressions of NDRG1 in NL and PNL tissues showed no evident difference. In ten cell lines, the highest expression was in the 293T human kidney cell line, the next one in liver cell L02, and the lowest one in the undifferentiated HLE cell line. Expression of NDRG1 in liver tissues enhanced with the development of the baby mouse and human fetus. Conclusion: NDRG 1 is probably related to the liver cell differentiation and is highly expressed in the specific stage of the differentiation. However, it could not be recognized as a marker of differentiation. The high expression of NDRG 1 in HCC indicates that HCC is not just distributed to a simple de differentiation. Much more study is necessary in understanding the comprehensive relationship of the disorder proliferation and differentiation of HCC and the related genes.

The all-in-one femtosecond laser corneal refractive surgery,especially small incision lenticule extraction (SMILE),has gained more and more attention in clinic,because of its small incision,“no flap” and corneal biomechanical stability advantages.However,like any other kind of corneal refractive surgery,the all-in-one femtosecond laser refractive surgery not only exists its clinical advantages,but also has its limitations.It is very important to put into perspective clinical advantages and limitations of all-in-one femtosecond laser corneal refractive surgery.

The effectiveness and efficiency of university educational management would have a direct impact on universities' teaching performance.Moreover,it is important to undertake a further reformation for the educational management system of university and college and establish such a standardized,high efficient educational management system.Since they would be the milestones that improve universities'teaching performance constantly and ensure there is a persistent evolution in universities as well.

With the development of modern medical technology,the traditional teaching mode can not satisfy the teaching requirements of biomedical measurement and instrument which is one of the main courses of biomedical engineering,and network teaching is the tendency.The article studies how to develop biomedical measurement and instrument's network teaching from many aspects,such as the construction of information platform,network teaching pattern design and so on,and the practice has achieved good results.