OBJECTIVE To discuss the occupational hazard factors and protection measures of Central Supply Unit.METHODS The occupational hazard factors in the Central Supply Unit were analyzed and some suggestion on the occupational protections to decrease occupational risk was done.RESULTS It is necessary to give some special training about the occupational safety,reinforce the precaution of occupational hazard with regard to biology,physics,chemistry and motor function to establish some protection and countermeasures.CONCLUSIONS The pertinent occupational protection strategy according to the actual hazard factors is more efficient and more benefit for high quality management of Central Supply Unit which will enhance the standardization,quality and working efficiency,and ensure the work safety and quality.

Objective To investigate the nalaxone treating first acute right heart failure.Methods52 patients with FARHF were divided into the naloxone group(30cases)and control group(20cases)at random.Patients in control group were given general comprehensively treating.Patients in naloxone group were given naloxone as well as comprehensively treating,were given intravenously 0.8 mg of first dose,if it was necessary,repeated 0.8 mg of dose,and continuously dropped for 1.2 mg was added into liquid 500 mL.After treatment of 24 hours,were compared the changes of HR、SBP、SV and heart function.ResultsIn the naloxone group,the total effective rates were 84.4%,the mortality rates were 6.6%;in control group.The total effective rates were 60%,the mortality rates were 18.3%.The difference of the effective rate of the naloxone group be treatment was statistically significant(P

Objective To study the expression and clinical significance of proliferating cell nuclear antigen(PCNA) and PTEN in endometrial carcinoma.Methods The expression of PTEN and PCNA of 18 cases of proliferative endometrium(normal group),21 cases of atypical hyperplasia of endometrium(precancerous lesion group),and 22 cases of endometrial carcinoma(endometrial carcinoma group) was determined immunohistochemically(SP method).Results PCNA expression in the normal group(16.1%),precancerous lesion group(71.4%),and endometrial carcinoma group(95.5%) was significantly different(P

Periodical assessment over the actual situation of physician work in a Class One Grade A hospital in Beijing is performed, and some ideas to improve the work are proposed in view of the problems existing in the work.

Objective To study the effect and safety of controlled-release dinoprostone on the induced labor of late pregnancy . Methods 60 cases of late pregnancy puerpera were treated in our hospital from February 2014 to September 2015, and divided into the control group and the experimental group with reference to the stochastic indicator method, 30 cases in each group. The control group were given induced labor by oxytocin, and the experimental group were given induced labor by controlled-release dinoprostone, Changes of Bishop score, effects of promoting cervical mature, induced labor situation, and adverse reaction were compared between two groups. Results After medication, the cervical Bishop score of the group (8.35±0.93) points were significantly higher than (6.64±0.72) of the control group (P<0.05). The total effective rate 96.67％(29/30) of promoting cervical mature in the experimental group was significantly higher than 80.00％ (24/30) of the control group (P<0.05). Medicine to clinical time (14.89±1.57)h and total labor time (6.94±1.52) h of the experimental group were significantly better than (21.48±2.35) h and (9.30±1.31) h of the control group (P<0.05). The rate of delivery by vagina of the experimental group 83.33％ (25/30) was significantly higher than 60.00％ (18/30) of the control group (P<0.05). There was no different on adverse reaction rate between two groups. Conclusion There was good effects of Controlled-release dinoprostone on the induced labor of later pregnancy, with high rate of delivery by vagina and better safety.

Antigens, Differentiation, B-Lymphocyte ; genetics ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; genetics ; metabolism ; Gene Fusion ; Gene Rearrangement ; Histocompatibility Antigens Class II ; genetics ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Lung Neoplasms ; drug therapy ; genetics ; metabolism ; Protein Kinase Inhibitors ; therapeutic use ; Protein-Tyrosine Kinases ; antagonists & inhibitors ; genetics ; metabolism ; Proto-Oncogene Proteins ; antagonists & inhibitors ; genetics ; metabolism ; Pyrazoles ; therapeutic use ; Pyridines ; therapeutic use ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; Sodium-Phosphate Cotransporter Proteins, Type IIb ; genetics

Objective To apply fluorescence-activated cell sorting (FACS) and magnetic activated cell sorting (MACS) in sorting natural killer (NK) cells from human peripheral blood. The efficiency of these two methods were compared so that the basic conditions for the further study on the lower proportion of peripheral blood cells could be created. Methods Twenty cases of normal peripheral blood and 3 cases of post-hematopoietic stem cell transplantation (post-HSCT) were collected and NK cell were sorted by FACS and MACS. Results Before sorting the normal peripheral blood, the proportion of the NK cell in the leukocyte was (12.86±3.62) %, the purity of NK cell was up to (96.15±2.03) % and the recovery was (95.08±2.16) %after sorting by FACS, the purity was up to (93.35±3.61) % and the recovery was (94.11±3.01) % by MACS.And before sorting the post-HSCT peripheral blood, the proportion of the NK cell in the leukocyte was (11.01±2.08) %, the purity of NK cell was up to (96.22±2.16) % and the recovery was (95.27±1.18) % after sorting by FACS. The purity was up to (90.98±1.94) % and the recovery was (94.54±3.52) % by MACS. Thereis difference in purity of NK cell (t = 5.925, P ＜0.05), while no difference in rate of recovery between 2methods (t = 0.789, P ＞0.05). Conclusion FACS is more efficient, rapidly and easily than MACS in sorting NK cell, especially for post-HSCT cases.

Objective To observe the clinical efficacy of abdominal acupuncture plus moxibustion in treating cervical spondylosis of vertebral artery type.Method A hundred patients with cervical spondylosis of vertebral artery type were randomized into a treatment group and a control group, 50 cases in each group. The control group was intervened by abdominal acupuncture plus electroacupuncture; while the treatment group was by moxibustion in addition to the treatment given to the control group. After a treatment course, the onset and remission time of vertigo was observed, and the clinical efficacies were compared between the two groups.Result The total effective rate was 96.0% in the treatment group versus 93.8% in the control group, and the difference was statistically significant (P<0.05). There was a significant difference in comparing the onset and remission time of vertigo between the two groups (P<0.05). In comparing the relapse rates 3, 6, and 12 months after the intervention between the two groups, the difference were statistically significant (P<0.01).Conclusion Abdominal acupuncture plus moxibustion is an effective approach in treating cervical spondylosis of vertebral artery type.

Objective: To observe the effect of electroacupuncture (EA) combined with Stretta radiofrequency treatment on contents of motilin (MTL) and gastrin (GAS) in gastroesophageal reflux disease (GERD) patients.
Methods: A total of 90 eligible GERD cases were randomly allocated into three groups, 30 in each group. Patients in the EA group were treated with EA, patients in the radiofrequency group were treated with Stretta radiofrequency, and patients in the observation group were treated with EA combined with Stretta radiofrequency. Assessment was made after a course of treatment.
Results: The recovery, improvement and failure cases and total effective rate in the EAgroup were 2, 18, 10 and 67.7% respectively, versus 13, 13, 4 and 86.7% in the radiofrequency group and 20, 9, 1 and 96.7% in the observation group. The therapeutic efficacy in the observation group was significantly better than that in the other two groups (P<0.01). After treatment, symptom scores of regurgitation, heartburn and substernal burning pain in all three groups were significantly reduced (P<0.05); the reduction in the observation group was more significant than that inthe other two groups (P<0.05),and the reduction in the radiofrequency group was more significant than that in the EA group (P<0.05). After treatment, the contents of MTL and GAS in all three groups were increased; and the increase in the observation group was more significant than that in the other two groups (P<0.05).
Conclusion: EA combined with Stretta radiofrequency treatment can significantly improve the clinical effect of GERD patients, improve regurgitation, heartburn and substernal burning pain and increase the contents of MTL and GAS.

AIM:Todetecttheeffectofspironolactoneonhyperthyroxine-inducedatrialremodeling.METH-ODS:New Zealand rabbits were divided into control group ( C ) , hyperthyroxine group ( H ) and spironolactone group ( S) .Thyroxin was given to the rabbits in group H and group S by intraperitoneal injection for 4 weeks , and then spirono-lactone was given in group S by gavage for 2 weeks.Atrial fibrillation (AF) was induced by burststimulation after ad-ministration.The inducing rate of AF and atrial effective refractory period ( AERP) were tested by intra-cardiac electro-physiologic instrument.The expression of AF-related Ca2+channel (Cav1.2), K+channels (Kv1.5 and Kv4.3) and con-nexins (Cx40 and Cx43) at mRNA and protein levels was detected by real-time PCR, immunohistochemistry and Western blot.RESULTS:Spironolactone reduced the inducing rate of AF .No significant difference of AERP between group H and group S was observed (P>0.05).Compared with group H, the protein expression of Cav1.2 was significant increased in group S (P<0.05).The expression of Kv1.5 at mRNA and protein levels in group S was significantly lower than that in group H (P<0.05), while the protein expression of Kv4.3 in group S was significantly decreased compared with group H (P<0.05).The mRNA expression of Cx43 in group S was significantly decreased compared with group H (P<0.01), whereas the protein expression of Cx 40 in group H was significantly higher than that in group S ( P<0.05 ) .CONCLU-SION:Spironolactone attenuates the hyperthyroxine-induced atrial remodeling in rabbits , and reduces the susceptibility of the myocardium to AF .