Objective To explore the factors associated with infections in children with leukemia after chem-otherapy. Methods Clinical data of fifty-six children with leukemia, who infected after chemotherapy were reviewed with infection characteristics and some related factors. Results The infection rates were 27% in upper airway,37% in lower upper airway,12% in oral cavity,5% in gastrointestinal tract,3% in perianal abscess,7% in urinary sys-tem,5% in scpficaemia and 2% in ofitis media. Most infection happened from five to ten days after chemotherapy,and the infection rates varied with chemotherapy scheme. The persistence time of infection were (8.5±2.2) days in the children with the numbers of neutrophil below 0.5×109/L and (3.5±1.4) days in those above 0.5×109/L with significant difference(P <0.01). Conclusions After chemotherapy,the children had more chances to be infected. To be more exact, the /nfection was more common observed in respiratory tract, next in gastrointestinal tract. And the stronger the medicine used the number of heterophil granulocyte dropped much more ,the infection happened more easily.

Objective To explore the effect of transforming growth factor ?_1 (TGF-?_1) on expression of paxillin(Pax) in human kidney proximal tubular epithelial cell line (HKC cells) in different phases. Methods HKC cells cultured in vitro were divided into four groups at random. Control group(C group): HKC cells were cultured with serum free medium (FSM), and TGF-?_1-treated groups (T1, T2, T3 group): HKC cells were cultured with FSM containing 10ng/ml TGF-?_1. In the latter groups, duration of treatment varied as follows: T1 group for 24h, T2 group for 48h, T3 group for 72h. The proliferation of HKC cells induced by TGF-?_1 was assessed by MTT and the expression of Pax was determined. The gene expression of Pax was determined by reverse transcripton polymerase chain reaction (RT-PCR) and the protein expression of Pax was assessed by immunohistochemical staining and Western blot. Results TGF-?_1 could induce the proliferation of HKC cells in a time-dependent manner. In C group there was expression of Pax mRNA and protein in HKC cells. The expression of Pax mRNA and protein in HKC cells was positive in all the T groups, especially in T2 group and T3 group they were significantly increased compared with T1 group (P

Objective To investigate the expression of Paxillin (Pax) in human kidney proximal tubular epithelial cell line (HKC) induced by transforming growth factor ?1(TGF?1). Methods HKC cells cultured in vitro were divided into three groups at random: control group(C): cultured with free serum medium(FSM), TGF?1-treated groups (T1 and T2): cultured with FSM containing different concentrations of TGF?1 (T1: 5 ng/ml, T2: 10 ng/ml). After 48-hour treatment, the expression of Pax mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining in HKC cells respectively. Results In Group C, the expression of Pax mRNA and protein in HKC cells was elementary. However, in T1 and T2 groups , the expression of Pax mRNA and protein in HKC cells was greatly increased, especially in T2 group which was more significantly increased than T1 group (P

Objective To explore the cognition processes of language in stroke patients by characterizing the differences between the event-related potentials (ERPs) N400 evoked bypicture-word andsentence-wordparadigms.Methods Twenty patients with a single brain lesion resulting from a first episode of stroke were tested along with 20 normal controls.Their N400 ERPs were tested using pictures and sentences as activating stimuli and phrases composed of nouns and verbs as the target stimuli.The N400 potentials were recorded from electrodes located at Fz,Pz,T3 and T4 positions of the International 10-20 system.The N400 latencies and amplitudes and the behavioral responses of the experimental and control groups were compared.Results The latencies were longer and the amplitudes were lower in response to sentence activation than to picture activation.The differences in terms of amplitude were the greatest with N400 recorded from Pz.The responses to the target stimuli were not significantly different with regard to the types of stimuli and recording location.The average response time was longer and the error rate was higher in response to sentence activation than to picture activation.Conclusion Multiple accesses,activating stimuli and integrating processes are involved in language cognition.The brain is more sensitive and accurate in response to picture stimuli in language cognition.The response to sentence stimuli is slower,and the error rate is higher.This suggests that it is better to usc varied prime stimuli in teaching children languages.

Objective To investigate the effect of Guizhi Fuling pills on levels of serum MAPK, ERK and VEGF in patients with endometriosis. Methods 60 patients with endometriosis were selected and according to the random control table were divided into the experiment group and the control group, 30 cases each.The control group were treated with conventional acetate goserelin sustained-release implantation agent subcutaneous injection, the experiment group were treated by Guizhi Fuling pill treatment.After treatment, the clinical efficacy, serum MAPK, ERK-5, VEGF, ERK, T-cad and VE-cad levels of the patients were compared.Results Compared with the control group, the clinical total effective rate was higher in the experiment group (P<0.05);MAPK and ERK-5 levels in serum were lower (P<0.05), ERK level in serum was lower(P<0.05);T-cad and VE-cad levels in serum were lower(P<0.05);VEGF level in serum was lower(P<0.05).Conclusion Guizhi Fuling pill can block the cell signal transduction pathway through inhibition of MEK and ERK activity, thereby inhibit abnormal endometrial proliferation and cell division cycle, can effectively inhibit the proliferation and differentiation of the tumor.

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently. With the enhanced staining efficiency, shortened test duration, simplified operation, increased positive detection rate and accuracy, the new technology lays a foundation of clinical definite, curative effect view and prognosis.

Objective To explore the effect of heroin dependence on the expression and the function of glucagon in islet A cells of rats.Methods The changes of numerical density on area of A cells and level of plasma glucagon of heroin-dependent rats were investigated by means of the immunohistochemical SABC method,image analysis and radioimmunoassay(RIA).Results As compared with SCG and NCG,it was observed that the number of Glu-IR positive cells increased and the intensity of immunohistochemical staining enhanced in the heroin-dependent groups.The result of image analysis showed that the numerical density on area of A cell apparently increased in the 38-day heroin-dependent group(P

Objective To design an intravenous indwelling needle in battle field aid.Methods Several concavity were made on the hollow tube inner surface posterior puncture needle case,and some corresponding convexity uplifted peripheral needle handle pistons posterior puncture needle core.Revolution could be avoided because the needle handle pistons were just embedded in hollow tube concavity.It was convenient to operate and decreased the operator's orientation transformation by the needle's handhold vane adopting rectangle instead of butterfly shape.Results The improved puncture needle was stable and the rate of success of intravenous puncture was increased.Conclusion The improved venous retaining needle can be applied in battle field aid.

Objective To study the efficacy as well as side effects of the Nimotop in the treatment of benign memory disorders with oral tab. Method 38 patients with benign memory disorders as treatment group, taking Nimotop oral tab 30mg, Tid for 6 weeks and anathr 34patients with benign memory disorders taking Pyrrolidone Acetamide oral tab 0. 8, Tid as control group. Result The total efficiency were 73.6% in treatment group, while 47% in control group. Although some improvement in the memory scale and memory quotient were found in both groups, but the treatment group is obviously superior to control group ( P ＜ 0. 05) . Conclusion Nimotop has positive effect on the treatment of benign memory disorders.