1.The effects of marrow mesenchymal stem cells transfected with hypoxia-inducible factor-1α gene
Tao TAN ; Jinfu YANG ; Feng LI ; Yifeng YANG ; Jianguo HU
Journal of Chinese Physician 2009;11(8):1013-1017
were obviously increased. Conclusion HIF-1α was successfully cloned. HIF-1α-pcDNA3.1 can be effectively transfected into MSCs with liposome-mediated method, which can result stable expression of HIF-1αin transfected MSCs.
2.Analysis of renal glucose threshold and related factors in patients with type 2 diabetes mellitus
Jinfu SHEN ; Zhuoqun WANG ; Shuangshuang FENG ; Mao LI ; Juan LI ; Tingting GAO ; Jingjing KANG ; Xingpo MA ; Min NIU
Chinese Journal of Endocrinology and Metabolism 2021;37(1):34-38
Objective:To investigate the value of renal glucose threshold and related factors in patients with type 2 diabetes mellitus.Methods:According to the cut-off point of normal renal glucose threshold(RT G 8.9-10 mmol/L), 107 patients with type 2 diabetes mellitus hospitalized in the Endocrinology Department of our hospital were divided into three groups: high RT G group(RT G>10 mmol/L), medium RT G group(8.9 mmol/L≤RT G≤10 mmol/L), and low RT G group(RT G<8.9 mmol/L). The clinical data and biochemical characteristics of each group were collected and analyzed. Results:The proportions of patients with high, medium, and low RT G of type 2 diabetes mellitus were 56%, 29%, and 15%, respectively. There were significant differences in RT G value, age, course of disease, body mass index(BMI), fasting plasma glucose(FPG), HbA 1C, total cholesterol(TC), serum creatinine, mean blood glucose(MBG), and 24-hour urine glucose between high and medium RT G groups. RT G, gender, BMI, FPG, HbA 1C, TC, and MBG in patients with high RT G group were different from those in low RT G group. Only RT G revealed a difference between medium and low RT G groups. Correlation analysis showed that RT G was positively correlated with gender, age, BMI, HbA 1C, TC, and low density lipoprotein-cholesterol(LDL-C). Regression analysis showed that BMI, HbA 1C, and LDL-C were the related factors affecting the RT G of patients with type 2 diabetes. Conclusion:There is a larger proportion of patients with high RT G in type 2 diabetes mellitus. Their BMI, HbA 1C, and LDL-C are associated with RT G in the patients with type 2 diabetes mellitus.
3.The temporal features of vertical spatial metaphor processing after moral disgust priming
Jinfu ZHU ; Wei CHEN ; Minghui PU ; Shenmei FENG
Chinese Journal of Behavioral Medicine and Brain Science 2018;27(3):266-271
Objective To explore the time characteristics and neural mechanism of processing vertical spatial metaphor after the activation of moral disgust,by applying event-related potential technique (ERPs) and semantic priming paradigm.Methods Totally 22 healthy college or graduate students were randomly selected from a university in Henan Province.A dual word priming paradigm was used to present priming words (moral disgust words and non moral disgust words).Then the target words (neutral words above or on the bottom of the screen) were presented,and the subjects were asked to judge the font structure of the target words.Processing feature was explored by investigating the differences in EEG indexes of different combinations.Results The interaction between the starting stimulation of the correct rate and the target stimulation in the behavior data was significant (F=20.40,P<0.01).In EEG data,compared with immoral aversion stimuli (P2:(0.81 ± 0.45) μV,N400:(-4.43 ± 0.58) μV),after the onset of moral abuses (P2:(-1.31 ± 0.40) μV,N400:(-5.04 ± 0.60) μV),the larger P2 (F =3.96,P< 0.05) and larger N400 (F =10.73,P<0.01) amplitudes were induced in the related brain regions after the emergence of neutral words at the bottom of the screen.Conclusion The analysis of behavior and EEG data indicates that,compared with immoral disgust,moral abuses show a higher semantic connection with metaphorical moral,and metaphorical immoral shows lower semantic connection.The individual is more inclined to metaphorical morality to relieve and release the feeling of disgust and maintain a good self-moral image.
4. Analysis of clinical characteristics and surgical results in patients with chronic subdural hematoma in different ages
Jinfu FENG ; Ping′an LI ; Bing ZHONG ; Ming GUO ; Yingjiao YU
Journal of Chinese Physician 2019;21(12):1790-1793
Objective:
To investigate the clinical characteristics and surgical results of patients with chronic subdural hematoma (CSDH) in different age groups.
Methods:
150 CSDH patients admitted to our hospital from January 2014 to January 2019 were selected as the research objects, and their clinical data were reviewed and collected. According to different age , they were divided into three groups: the <55 year old group (
5.Effect of blood glucose control on level of lactic acid in patients with cardic-valve replacement.
Jiefeng YU ; Tao TANG ; Feng LIU ; Jianguo HU ; Ling JIANG ; Jinfu YANG
Journal of Central South University(Medical Sciences) 2009;34(5):443-447
OBJECTIVE:
To evaluate the effect of different control levels of glucose on the serum lactic acid during operation, and to investigate the relation between glucose and lactic acid to find a new way of myocardial protection.
METHODS:
Volunteers were divided into an experiment group(n=38) and a control group(n=33) by random sampling and double blind method. The experiment group received intensive insulin therapy and the control group received traditional therapy. The arterial blood gas samples of all the patients at different time points after the operation were harvested in the intensive care unit for blood gas analysis. The related data were collected and analyzed.
RESULTS:
The serum glucose level in the 2 groups decreased firstly, then increased, and recovered finally. The serum lactic acid level in the 2 groups increased firstly, decreased later, then reincreased, and recovered finally. The highest level of the serum lactic acid was found 2 hours after the operation. There were significant differences in serum glucose and lactic acid levels at 2, 12, and 24 h after the operation in the two groups (P<0.01). The other data were not significant (P>0.05).
CONCLUSION
The variation of serum glucose and lactic acid level at 2, 12, 24 h after the valve replacement is consistent and significant. The serum lactic acid in the serum may be decreased by controlling the blood glucose, which provides experiment basis for myocardial protection.
Adult
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Cardiopulmonary Bypass
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adverse effects
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Double-Blind Method
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Female
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Heart Valve Prosthesis Implantation
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Humans
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Hyperglycemia
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blood
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drug therapy
;
prevention & control
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Insulin
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therapeutic use
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Lactic Acid
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blood
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Male
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Middle Aged
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Postoperative Complications
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drug therapy
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prevention & control
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Rheumatic Heart Disease
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blood
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surgery
6.Analysis of CYP21A2 gene mutations among patients with classical steroid 21-hydroxylase deficiency.
Yueqing SU ; Hanqiang CHEN ; Wenbin ZHU ; Jing WANG ; Jinfu ZHOU ; Yao CHEN ; Hong ZHAO ; Yinglin ZENG ; Feng LIN ; Honghua ZHANG ; Qingying LIN
Chinese Journal of Medical Genetics 2016;33(6):786-791
OBJECTIVETo assess the frequencies of CYP21A2 gene mutations among patients from Fujian area with classical 21-hydroxylase deficiency.
METHODSFor 19 probands from different families affected with classical steroid 21-hydroxylase deficiency and 74 family members, mutations of the CYP21A2 gene were analyzed with combined nested polymerase chain reaction, Sanger sequencing and multiplex ligation-dependent probe amplification. Time resolved fluorescence immunoassay was performed to determine the level of 17-hydroxyprogesterone (17-OHP) in all family members. Clinical data and laboratory results of the probands and their family members were analyzed.
RESULTSEleven mutations were identified among the 38 alleles from the 19 probands. 92.1% (35/38) of the mutant CYP21A2 alleles were due to recombination between CYP21A2 and CYP21A1P. Gene conversion and deletions were identified in 84.2% (32/38) and 7.9% (3/38) of the alleles, respectively. IVS2-13A/C>G and chimeras were the most common mutations, which respectively accounted for 34.2% (13/38) and 18.4% (7/38) of all mutant alleles. Among these, IVS2+1G>A and Q318X+356W were first reported in China. 74.3% (55/74) of the family members were carriers of heterozygous mutations. However, no significant difference was found in the 17-OHP levels between carriers and non-carriers (P>0.05).
CONCLUSIONThere seems to be a specific spectrum of CYP21A2 gene mutations in Fujian area, where IVS2-13A/C>G and chimeras are the most common mutations.
Adrenal Hyperplasia, Congenital ; genetics ; Alleles ; Female ; Humans ; Male ; Mutation ; genetics ; Steroid 21-Hydroxylase ; genetics
7.Genetic analysis of 36 children affected with phenylalanine hydroxylase deficiency from Fujian.
Wenbin ZHU ; Hanqiang CHEN ; Yueqing SU ; Hong ZHAO ; Jing WANG ; Jinfu ZHOU ; Yao CHEN ; Yinglin ZEN ; Feng LIN ; Honghua ZHANG
Chinese Journal of Medical Genetics 2015;32(2):158-162
OBJECTIVETo study the characteristics of phenylalanine hydroxylase gene (PAH) mutations in patients with PAH deficiency in Fujian population.
METHODSPeripheral blood samples of 36 patients and their parents with classical type phenylketouria (PKU) were collected. Genomic DNA was extracted. Following PCR amplification, DNA sequencing was carried out to identify the origins of mutations.
RESULTSTwenty types mutations were identified in 63 of the 72 alleles. The most common mutations were R241C, R408Q and Ex6-96A>G, which respectively accounted for 15.9%, 12.7% and 11.1% of all mutant alleles. The c.189_190dupTGAC mutation was first reported. R241C was associated with 28% of mild hyperphenylalaninemia and R408Q is associated with 25% of classical PKU.
CONCLUSIONThere is a specific spectrum of PAH gene mutation in Fujian region. R241C, R408Q and Ex6-96A>G are the most common mutations.
Adolescent ; Alleles ; Asian Continental Ancestry Group ; genetics ; Base Sequence ; Child ; Child, Preschool ; China ; Female ; Genotype ; Humans ; Male ; Molecular Sequence Data ; Mutation ; Phenylalanine Hydroxylase ; genetics ; Phenylketonurias ; enzymology ; genetics
8.Unidirectional valved patch for congenital heart disease with severe pulmonary hypertension.
Ming WU ; Jinfu YANG ; Yifeng YANG ; Jianguo HU ; Xinmin ZHOU ; Feng LIU ; Zhongshi WU ; Tianli ZHAO ; Lian XIONG ; Xin WANG ; Ni YIN
Journal of Central South University(Medical Sciences) 2011;36(11):1097-1101
OBJECTIVE:
To explore the effect of unidirectional valved patch (UVP) for congenital heart disease (CHD) with severe pulmonary hypertension (PH).
METHODS:
We retrospectively analyzed the treatment of 37 CHD patients with severe PH by UVP in the operation, and summarized its short-term to mid-term effect to find an optimum therapeutic regimen.
RESULTS:
Before the operation, the ECG showed that the mean pulmonary artery pressure (MPAP) ranged 65-72 mmHg, and the cardiac catheterization showed the pulmonary artery pressure ranged 80-120 mmHg, P(P)/P(A) ranged 0.8-1.05,PVR ranged 8.5-19.2 (under oxygen inhalation 6.8-14.6) wood unit.After the operation, P(P)/P(A) ranged 0.4-0.72 on weaning-off CPB. Postoperative ECG showed the MPAP ranged 32-48 mmHg. No pulmonary hypertension crisis occurred and no patient died. Mechanical ventilation time ranged from 32 h to 8 d and the SaO₂ ranged 93%-96% at rest after the extubation.The right-to-left shunt situations by ECG were as follows:22 cases had shunt 5 d after the operation, 11 cases had shunt 1 month after the operation,4 cases 3 months after the operation, and none 1 year after the operation but one patient lost follow-up.However,there were no long-term follow-up data: 12 patients had a 1-year follow-up, 5 patients had a 3-year follow-up, and most patients had just 3-month follow-up.
CONCLUSION
UVP can decrease the operative risk in CHD with severe PH at perioperative period. The short-term to mid-term effect is satisfactory, while long-term effect remains uncertain.
Adolescent
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Cardiac Surgical Procedures
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methods
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Child
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Female
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Heart Defects, Congenital
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complications
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surgery
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Heart Septal Defects, Ventricular
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complications
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surgery
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Humans
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Hypertension, Pulmonary
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etiology
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surgery
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Male
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Pericardium
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transplantation
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Prosthesis Implantation
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Retrospective Studies
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Young Adult
9.Analysis of GCDH gene mutations in 3 patients from Fujian area with glutaric academia type I.
Yao CHEN ; Qingying LIN ; Yinglin ZENG ; Hong ZHAO ; Weifen CHEN ; Jinfu ZHOU ; Yueqing SU ; Feng LIN ; Honghua ZHANG ; Wenbin ZHU
Chinese Journal of Medical Genetics 2018;35(5):657-660
OBJECTIVETo explore clinical features and mutation types in patients from Fujian area with glutaric academia type I(GA I).
METHODSSerum acylcarnitine and urine organic acid of 3 patients were determined with tandem mass spectrometry and gas chromatographic mass spectrometry. The patients also underwent magnetic resonance imaging analysis for the cranial region. Genomic DNA was extracted from peripheral blood samples, and the 12 exons and flanking regions of the GCDH gene were amplified with PCR and subjected to direct DNA sequencing. One hundred healthy newborns were used as controls.
RESULTSMutations of the GCDH gene were identified in all of the 3 patients. Two patients have carried compound heterozygous mutations including c.1244-2A>C and c.1147C>T(p.R383C), c.406G>T(p.G136C) and c.1169G>A(p.G390E), respectively. One has carried homozygous c.1244-2A>C mutation. The same mutations were not detected among the 100 healthy newborns. Only one patient received early intervention and did not develop the disease. The other two had irreversible damagesto their intelligence.
CONCLUSIONc.1169G>A(p.G390E) is likely pathogenic mutations for GA I patients from Fujianarea. Early screening of neonatal metabolic diseases is crucial for such patients.
10.Association of thyroperoxidase gene polymorphisms with dyshormonogenesis in congenital hypothyroidism.
Yueqing SU ; Jing WANG ; Jinfu ZHOU ; Yao CHEN ; Hong ZHAO ; Yinglin ZENG ; Feng LIN ; Honghua ZHANG ; Wenbin ZHU ; Hanqiang CHEN
Chinese Journal of Medical Genetics 2015;32(6):861-865
OBJECTIVETo assess the association of thyroperoxidase (TPO) gene polymorphisms with dyshormonogenesis in congenital hypothyroidism (CH).
METHODSThe 17 exons and flanking introns of the TPO gene from 30 randomly selected samples were sequenced for the selection of single nucleotide polymorphisms (SNPs). In 136 patients with dyshormonogenetic CH and 141 healthy controls from the same region, the selected SNPs were genotyped by polymerase chain reaction (PCR) and direct sequencing or PCR-restriction fragment length polymorphism (RFLP).
RESULTSSix SNPs (rs9678281, rs376413622, rs1126797, rs4927611, rs732609 and rs1126799) were selected to determine the genotype for each sample. Among these, rs4927611 and rs732609 showed a significant difference between the two groups in both allelic and genotypic frequencies. With a recessive model of inheritance, rs732609 CC (OR=0.484, 95%CI: 0.253-0.927, P=0.04) and rs4927611 TT (OR=0.32, 95%CI: 0.112-0.915, P=0.047) were greater in the patients.
CONCLUSIONrs4927611 and rs732609 may be associated with dyshormonogenetic CH. rs4927611 TT and rs732609 CC are genotypes associated with potential risk for the disease.
Alleles ; Autoantigens ; genetics ; Base Sequence ; Child, Preschool ; Congenital Hypothyroidism ; blood ; genetics ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; genetics ; Genotype ; Humans ; Infant ; Infant, Newborn ; Iodide Peroxidase ; genetics ; Iron-Binding Proteins ; genetics ; Linkage Disequilibrium ; Male ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide ; Risk Factors ; Thyrotropin ; blood ; Thyroxine ; blood