Objective: To study the efficacy and clinical experience of surgical treatment in the patients with left main coronary artery stenosis. Methods: Two hundred and twenty-five patients with left main stenosis (LMS) underwent coronary artery bypass graft (CABG) between January 1999 and June 2003. The average age was 63.4 years. The left ventricular ejection fraction was ≤30% in 11 patients. Emergency surgical revascularization was performed in 6 patients because of hemodynamic instability in whom 3 were treated with intraaortic balloon pump (IABP) before surgery. Seventy-six patients underwent coronary artery revascularization with conventional cardiopulmonary bypass, 149 without pump (OPCAB), and 5 patients were converted to CPB. Thirteen patients accepted total arterial revascularization and 3 total venous conduits. Results: The mean preoperative stay was 2.3 days and number of distal anastomoses was 2.95/pt. There were 13 postoperative deaths with a mortality of 5.78%. Conclusion: The presence of left main stenosis is an independent predictor of postoperative mortality after coronary bypass grafting. CABG at presents is the first choice for such patient and is a safe and effective method.

OBJECTIVE：To investi gate the potential mechanism of couplet medicine of Cuscutae Semen-Lycii Fructus in the treatment of premature ovarian failure. METHODS ：Main active components and related targets of couplet medicine of Cuscutae Semen-Lycii Fructus in the treatment of premature ovarian failure were obtained from TCMSP ，GeneCards and OMIM database. The intersection genes between them were screened using Venn online tool. Cytoscape 3.7.0 software was adopted to establish the active ingredients-target network and the PPI network. GO and KEGG pathway enrichment analysis on intersection genes were carried out by DAVID database. Finally ，an active component-target-key pathway network was constructed. RESULTS ：Totally 42 active components ，231 and 1 913 targets for active components and disease were obtained from couplet medicine of Cuscutae Semen-Lycii Fructus. The components with high node degree included quercetin ，kaempferol，β-sitosterol，isorhamnetin，glycitein， stigmasterol and sesamin ，etc. There were 149 intersection genes between the active component targets and premature ovarian failure targets. PPI network contained 149 nodes and 2 970 edges，with an average node degree of 39.9 and an average medium of 0.005 4. The results of GO analysis showed that molecule function of the above-mentioned genes mainly involved protein binding ， enzyme binding ，etc. Biological process mainly included that positive regulatio n of transcription from RNA polymerase Ⅱ promoter，positive regulation of transcription DNA-templated ， Cell components mainly included nucleus ，cytoplasm，etc. Signaling pathway mainly involved cancer signaling pathway ， hepatitis B signling pathway ，PI3K/AKT signaling pathway ， MAPK signaling pathway ， etc. The results of active 617693370@qq.com component-target-key pathway network showed that active components of Cuscutae Semen and Lycii Fructus were flavonoids and alcohols ；key target included AKT 1，TP53， VEGFA，IL6，TNF，etc. Signaling pathway mainly involved cancer signaling pathway ，hepatitis B signaling pathway ，PI3K/AKT signaling pathway ，MAPK signaling pathway ，etc. CONCLUSIONS ：Through PI 3K/AKT signaling pathway and MAPK signaling pathway，the active components of couplet medicine of Cuscutae Semen-Lycii Fructus may act on AKT 1，TP53 and other targets ， and then play a therapeutic role on premature ovarian failure. The Potential active components stigmasterol ，sesamin and potential targets IL 6，TNF were found.