Autoimmune Diseases ; Humans ; Pharyngeal Diseases ; Ulcer

OBJECTIVE: To study the effect of IgE, GM-CSF on eosinophil accumulation in the nasal polyps and find the relationship between them. METHOD: Thirty mucosa constitution samples of nasal polyps and eleven inferior nasal concha were observed. The distribution of IgE was detected by SP immunohistochemical method, while the concentration of GM-CSF was measured by ELISA technique and the number of eosinophils were counted in the same tissues. RESULT: The level of IgE, GM-CSF and eosinophils had significant different between the nasal polyps and control group (P < 0.01). There was positive correlation between IgE/GM-CSF and the number of eosinophils in the nasal polyps group (r = 0.65, 0.62, P < 0.01). The correlation between concentration of IgE and GM-CSF was also significant in nasal polyps group (r = 0.52, P < 0.01). CONCLUSION Nasal polyps is characterized by eosinophil accumulation. IgE and GM-CSF may contribute to the recruitment of eosinophils in nasal polyps. Type-I allergy mediated by IgE play an important role in the pathogenesis of nasal polyps.
Adolescent ; Adult ; Aged ; Eosinophils ; pathology ; Female ; Granulocyte-Macrophage Colony-Stimulating Factor ; metabolism ; Humans ; Immunoglobulin E ; metabolism ; Male ; Middle Aged ; Nasal Mucosa ; metabolism ; pathology ; Nasal Polyps ; metabolism ; pathology ; Young Adult

OBJECTIVE: To study the expression of Survivin, Caspase-3 and p53 in surgical margin of laryngeal squamous cell carcinoma (LSCC), and their clinical significance and relationship. METHOD: Immunohistochemistry method was used to detect the expression of Survivin, Caspase-3 and p53 in 116 cases of surgical margin of LSCC. RESULT: According to the recurrence, the patients were divided into four group, recurrence within one year, between one year to three years, between three to five years, more than 5 years (include no recurrence). The expression rate of survivin were 92.9%, 85.0%, 50.0% and 21.1% (P < 0.01) in four groups, respectively. The expression rate of Caspase-3 were 50.0%, 50.0%, 66.7% and 89.5% (P < 0.01). The expression rate of p53 were 57.1%, 37.5%, 45.8% and 18.4% (P < 0.05). The expression rate of survivin, caspase-3 and p53 in four groups, respectively, there were significant differences (P < 0.05). CONCLUSION Expression of Survivin, Caspase-3 and p53 in surgical margin of LSCC may play an important role in the tumorigenesis and development of laryngeal carcinoma. They are feasible for early diagnosis and prognosis estimation.
Aged ; Carcinoma, Squamous Cell ; metabolism ; pathology ; surgery ; Caspase 3 ; metabolism ; Female ; Humans ; Inhibitor of Apoptosis Proteins ; metabolism ; Laryngeal Neoplasms ; metabolism ; pathology ; surgery ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Prognosis ; Survivin ; Tumor Suppressor Protein p53 ; metabolism