Objective:To evaluate the efficacy and safety of the micro-crosslinked hyaluronic acid injected by Shuiguang Needle Injection in facial rejuvenation.Methods:Thirty-two patients were enrolled. In this split face study, micro-crosslinked hyaluronic acid and non-crosslinked hyaluronic acid were randomly applied in the treated side of the face. Procedure efficacy (judged by VISIA), complication and subjects＇ satisfaction were assessed before treatment, one month, two months and three months after treatment.Results:According to the VISIA, 32 cases were effective one month after treatment on the treatment side, accounting for 100%; 28 cases were effective three months after the end of treatment, accounting for 87.51%. On the control side, 28 cases were effective one month after treatment, accounting for 87.50%, and 7 cases were effective after three months, accounting for 21.88%. There was no statistically significant difference in complications between the left and right sides; 31 patients were satisfied on the treatment side, accounting for 96.88%, and 28 patients on the control side were satisfied, accounting for 87.50%.Conclusions:The micro-crosslinked hyaluronic acid injected by Shuiguang Needle Injection appears to be effective and durable for facial rejuvenation. And it has a low complication rate and high subject satisfaction.