AIM: To investigate the effects of oxidized low density lipoprotein (OX-LDL) on expression of matrix metalloproteinase-2 (MMP-2) in human umbilical vein endothelial cells (HUVEC). METHODS: The MMP-2 mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR), MMP-2 protein was measured by western blotting and MMP-2 activity in conditioned medium was observed by gelatin zymography. RESULTS: In comparison with the control, there was no difference in the expression of MMP-2 between control and 25 mg/L LDL, whereas 25 mg/L OX-LDL increased MMP-2 activity in HUVEC ( P

AIM To explore whether or not the function of insulin like growth factor (IGF I) in promoting osteopontin gene expression and protein synthesis of rat vascular smooth muscle cell (VSMC). METHODS Cultured cells were incubated with different concentration of IGF I for different hours, then RT PCR and Western blotting analysis were used to observed the effects of IGF I on osteopontin expression of rat vascular smooth muscle cells RESULTS IGF I (20 ?g?L -1 ) could obviously induce the gene expression and protein synthesis of osteopontin, RT PCR results showed that the ratio of mRNA of osteopontin and ? actin in cells incubated with IGF I (20 ?g?L -1 ) for 3, 24 and 48 h increased by 39 70%,40 44% and 54 05% respectively, as compared with that of the control ( P

Aim To elucidate the polymorphism of hepatic lipasegene gene and the relation to coronary heart disease. Methods CHD group had one hundred and fifty-six patients, and each subgroup was: myocardium infarction CHD subgroup included eighty-four patients; non-myocardium infarction subgroup included seventy-two patients; pure CHD subgroup comprised sixty-five patients and hypertension and CHD subgroup comprised ninety-one patients. Phenol-Chloroform method was used to extract DNA from human peripheral blood, and a combination of polymerasechain reaction and restriction fragment length polymorphism were used to analyze the distribution of genotypes and alleles of the polymorphism site of hepatic lipase. Results The genotype and allele distribution of HL-514C/T polymorphism were significantly different between the whole CHD group and control group(P

OBJECTIVETo investigate the plasma levels of coagulation factor VII (FVII) and polymorphisms of FVII gene in patients with coronary heart disease (CHD), and evaluate the effect of plasma FVII levels and FVII gene polymorphisms on CHD.

METHODSPlasma FVIIa, FVII: Ag and FVIIc were measured and polymorphisms of FVII gene were analyzed in 149 control cases and 60 CHD cases, including 33 acute myocardial infarction (AMI) cases by a combination of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and agarose gel electrophoresis.

RESULTSFVIIa, FVIIc in AMI group were significantly higher than that in control group, but FVII: Ag wasn't. There were no significant difference in plasma FVIIa, FVII: Ag and FVIIc between CHD and control group. The IVS7 genotypic frequency in AMI group was significantly different from that in control group. There was no significant difference in genotypic frequencies and allelic frequencies in other polymphism sites. FVII: Ag was significantly higher in -402A homozygote than that in -402G homozygote.

CONCLUSIONSIncreased FVII levels, especially FVIIa and FVIIc in plasma, may contribute to coronary artery thrombosis. There was significant difference in IVS7 genotype frequency between control and AMI groups, but the rest weren't significantly different. FVII: Ag was significantly higher in -402A homozygote than that in -402G homozygote. Polymorphism of -402 G/A may play an indirect role in AMI by regulating plasma FVII levels.

Coronary Disease ; blood ; genetics ; Factor VII ; analysis ; genetics ; Female ; Genotype ; Humans ; Male ; Middle Aged ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Polymorphism, Restriction Fragment Length

Objective:To investigate the clinical efficacy and safety of the triple-arc buried wire method for facial lift surgery.Methods:This study retrospectively included 55 patients who underwent facial lift surgery using the triple-arc buried wire method at Nanjing Medical University Friendship Plastic Surgery Hospital from May 2018 to May 2021, and consisted of 1 male and 54 females, with an age range of 28 to 55 (37.9±7.2) years. Patients were positioned either standing or sitting, with markings made for the suture pathways, entry points, and exit points. The first safety line extended from the outer canthus of the eye to the earlobe, while the second safety line followed the lateral aspect of the nasal artery to form a vertical line with the nose; the aesthetic line was drawn extending 2 cm outward from the oral commissure. Local infiltration anesthesia with a 2% lidocaine and epinephrine solution was applied at the marked entry and exit points, followed by subcutaneous tunnel infiltration anesthesia along the designed suture pathway. Three dual-direction barbed absorbable sutures were deployed in an arc along the midface, repositioning the malar fat pads. Postoperative follow-ups were conducted immediately after surgery and at 1, 6, and 12 months to assess patient satisfaction. The wrinkle severity rating scale (WSRS) was recorded to evaluate the appearance of the nasolabial folds. The incidence of postoperative complications such as hematoma, tightness, asymmetry, and exposed sutures was also documented.Results:All 55 patients experienced significant midface lifting effects immediately after surgery, resulting in a fuller appearance and shallower nasolabial folds. The follow-up rates immediately postoperatively and at 1, 6, and 12 months were 100.0% (55/55), 100.0% (55/55), 94.6% (52/55), and 89.1% (49/55); patient satisfaction rates were 98.2% (54/55), 98.2% (54/55), 98.2% (54/55), and 98.2% (54/55), respectively. The preoperative WSRS score for the 55 patients was 3 (2, 4), while the score at immediately after surgery was 2 (1, 3), indicating a statistically significant difference ( P<0.001). No significant complications such as hematoma, tightness, asymmetry, or exposed sutures were noted in any patients. Two patients developed noticeable ecchymosis on both cheeks within 24 hours postoperatively, which resolved spontaneously after 3 weeks, with facial appearance and chewing function returning to normal after 1 month. One patient had a localized sunken area at the exit point, which self-resolved after 11 months. Conclusions:The triple-arc buried wire method for facial lift surgery is safe and effective, with minimal complications.