BACKGROUND: Photodynamic antimicrobial chemotherapy holds broad-spectrum antibacterial ability, fast onset of action and non-resistance, which has been extensively applied in the treatment of superficial localized infection. OBJECTIVE: To explore the efficacy of photodynamic antimicrobial chemotherapy for treating osteomyelitis. METHODS: Thirty-six New Zealand white rabbits (provided by Laboratory Animal Center of Academy of Military Medical Sciences) were selected, and a left tibia osteomyelitis model was established. At 28 days after modeling, rabbit models were randomly divided into three groups: Blank group (tibia debridement only), control group (vancomycin/polymethylmethacrylate bone cement was filled into the intramedullary cavity of the infectious tibia after debridement), and experimental group (intramedullary treatment of photodynamic antimicrobial chemotherapy after debridement). The gross observation, imaging examination and bacterial culture were performed at 4, 8, and 12 weeks after surgery. The study was approved by the Ethical Committee of Affiliated Hospital of Logistics College of CAPF, with the approval No. (2015)-0002. RESULTS AND CONCLUSION: (1) Appearance of the skin: Purulent secretion was observed in the blank group, but disappeared in the control and experimental groups where the skin healed well. (2) X-ray examination: With time going, the osteomyelitis aggravated in the blank group. The bone destruction was reduced gradually in the control and experimental groups, and the bone defects healed gradually. There was no significant difference between control and experimental groups at different time points after surgery. (3) Bacterial culture: With time increasing, the bacterial positive rate showed no significant change in the blank group. The bacterial positive rate in the control and experimental groups was on a decline, which showed no significant difference between control and experimental groups at different time points after surgery (P > 0.05). (4) These results indicate that photodynamic antimicrobial chemotherapy is a new treatment for osteomyelitis to effectively control infection, providing experimental basis in clinical practice.

ObjectiveTo explore the treatment efficacy and feasibility of large dose of cytidine diphosphate choline (CDPC) in cardiopulmonary cerebral resuscitation.MethodsOne hundred and thirtytwo patients with sudden cardiac arrest successfully treated by the cardiopulmonary cerebral resuscitation were divided into treatment group ( 75 patients) and control group (57 patients) by random number tables.Two groups of patients were given adrenaline resuscitation drugs,reducing the intracranial pressure drugs,hypothermia,hormones and other basic treatment.Control group used CDPC 0.25 g with glucoseinjection 300 ml to do intravenous drip (once a day,21 days as a period of treatment),and treatment group added CDPC to 2.00 g.The patients were treated by basic traditional analeptic.The Glasgow coma scale (GCS) was carried out in two groups in admission to hospital and 3,7,14,21 days after treatment,and the changes of lysophosphatidic acid (LPA) and phosphatidic acid (AP) level were monitored.ResultsThe GCS score was significantly higher in treatment group 7,14,21 days after treatment(P ＜ 0.05 or ＜ 0.01 ),but GCS score had no significant difference before and after treatment in control group(P ＞ 0.05).The level of LPA in treatment group was significantly decreased 14 days after treatment [ (2.98 ± 0.28) μmol/L ] and 21 days after treatment[ (1.85 ± 0.21)μmol/L],and had significant difference compared with that admission to hospital [ (3.75 ± 0.22) μ mol/L ] (P ＜ 0.05 or ＜ 0.01 ).In control group,there was no significant difference before and after treatment (P ＞0.05).The level of AP in treatment group 21 days after treatment was significantly decreased compared with that in control group [ ( 3.45 ± 0.23 ) μ mol/L vs.( 5.20 ± 0.21 ) μ mol/L ](P ＜ 0.01).ConclusionLarge dose of CDPC can improve neurological function of cardiopulmonary cerebral resuscitation,which is a safe,effective and easy way to be popularized.

BACKGROUND:In foreign animal experiments and clinical reports, microporous polysaccharide hemospheres are characterized as good hemostatic effect, no delayed bleeding, good biocompatibility, and no adverse reactions in the body. OBJECTIVE:To assess the efficacy and safety of microporous polysaccharide hemospheres on reducing blood loss after unilateral total hip arthroplasty. METHODS:Totaly 98 patients who received unilateral total hip arthroplasty due to femoral neck fractures, 39 males and 59 females, aged 61-77 years, were randomly divided into two groups: test group, treated with 1g microporous polysaccharide hemospheres before suture of wound; control group, treated with electric coagulation hemostasis before suturing. Drainage tubes were placed in the two groups. Hemoglobin, blood loss, alogeneic transfusion rate, drainage volume, coagulation index and incidence of deep venous thrombosis of the lower limbs were compared. RESULTS AND CONCLUSION:The total blood loss, drainage volume, and transfusion rate in the test group were significantly lower than those in the control group (P < 0.01), but there were no differences in D-dimer, platelet count, activated partial thromboplastin time and international normalized ratio between the two groups. One case of each group developed deep venous thrombosis of the lower limbs, and no significant difference was in the incidence of deep venous thrombosis between the two groups. No infection occurred. These findings indicate that microporous polysaccharide hemospheres that are safe, effective and convenient can reduce the visible and hidden blood loss after unilateral total hip arthroplasty.

Objective To evaluate the Imaging diagnosis of SARS in death case.Methods 11 death patients of SARS in keeping with criteria from April to May 2003 were enrolled into the study. Imaging characteristics of chest x-ray film and CT in these patients were analysed.Results All cases were positive on chest X-ray film and CT,the lung shadows were marked.The extent of the pathological changes in lungs was 80%～90% in 6 cases and 90% above in 5 cases,multilobcs of bilateral lungs were involved in all cases.The lesions were diffuse spotty shadow or shaggy cloudy shadow in 8 cases,ground-glass opacification in one case and bronchogram structures could be seen.Conclusion Imaging findings in the cases with severe SARS mostly manifest as effusion,consolidation and interstitial inflammation in multilobes of bilateral lungs.

OBJECTIVES: To explore the effects of hypoxic and hypobaric conditions on blood gas and erythrocyte-related indicators in rats. METHODS: SD male rats were exposed to low-pressure hypoxic conditions simulating an altitude of 6500 m in a small or a large experimental cabin. Abdominal aortic blood samples were collected and blood gas indicators, red blood cells (RBCs) count, and hemoglobin (Hb) content were measured. The effects of exposure to different hypoxia times, different hypoxia modes, normal oxygen recovery after hypoxia, and re-hypoxia after hypoxia preconditioning on blood gas indicators, RBCs count and Hb content were investigated. RESULTS: The effect of blood gas indicators was correlated with the length of exposure time of hypoxia and the reoxygenation after leaving the cabin. Hypoxia caused acid-base imbalance and its severity was associated with the duration of hypoxia; hypoxia also led to an increase in RBCs count and Hb content, and the increase was also related to the time exposed to hypoxia. The effects of reoxygenation on acid-base imbalance in rats caged in a small animal cabin were more severe that those in a large experimental cabin. Acetazolamide alleviated the effects of reoxygenation after leaving the cabin. Different hypoxia modes and administration of acetazolamide had little effect on RBCs count and Hb content. Normal oxygen recovery can alleviate the reoxygenation and acid-base imbalance of hypoxic rats after leaving the cabin and improve the increase in red blood cell and hemoglobin content caused by hypoxia. The improvement of hypoxia preconditioning on post hypoxia reoxygenation is not significant, but it can alleviate the acid-base imbalance caused by hypoxia in rats and to some extent improve the increase in red blood cell and hemoglobin content caused by hypoxia. CONCLUSIONS Due to excessive ventilation and elevated RBCs count and Hb content after hypoxia reoxygenation, oxygen partial pressure and other oxygenation indicators in hypoxic rats are prone to become abnormal, while blood gas acid-base balance indicators are relatively stable, which are more suitable for evaluating the degree of hypoxia injury and related pharmacological effects in rats.
Rats ; Animals ; Male ; Acetazolamide ; Hypoxia ; Oxygen ; Erythrocytes ; Hemoglobins ; Acid-Base Imbalance