Humans ; Orthodontics ; Periodontitis ; therapy

Decision Making ; Dental Implantation, Endosseous ; Dental Implants ; Humans ; Periodontitis

Objective:To study the different gene expressions in human periodontal cells after being treated with Emdogain(EMD). Methods: The fourth passage periodontal cells were cultured. Cells in experimental group were cultured with 100 μg/ml EMD in non-serum DMEM for 3 days. Cells in control group were cultured in non-serum DMEM for 3 days. The gene expressions in human periodontal cells were screened with gene chip technique. Results: There were 112 differently expressed genes, 11 down-regulated genes and 101 up-regulated genes. Conclusion: After treated with Emdogain, expression of genes related to cell growth, cell proliferation, biosynthesis of extracellular matrix, angiogenesis and osteogenesis is up-regulated dramatically. Genes related to lipid metabolization and keratinocyte growth is down-regulated in human periodontal cells.

Objective: To investigate the capability of Streptococcus sanguis(S.s) producing hydrogen peroxide (HP) and screen the strains of S.s with high yield of hydrogen peroxide.Methods: S.s were isolated from supergingival plaque of people with healthy periodontium. The microorganisms were cultivated anaerobically or aerobically, then washed and incubated with KCl buffer. Hydrogen peroxide and bacterial proteins were assayed spectrophotometrically. S.oralis ATCC 10557 was used as the controls. Results: In anaerobical culture the HP production (nmol/?g) of S.s and S.oralis was 118.22?69.92 and 338.60?192.14( P

Objective:To study the relationship between human cytome ga lovirus (HCMV) and chronic periodontitis. Methods: In 62 chronic periodontitis patients (27 male and 35 female), subgingival samples were colle cted from active periodontitis site,quiescent periodontitis site, and initial g ingivitis site of each subjects. Human cytomegalovirus was detected by nested - polymerase chain reaction method. Results: HCMV detectable ratio in the active periodontitis sites was significantly higher than that in the qui escent sites (38.7% VS 14.5%, P0.05).Conclusion:HCMV infection may be associated wi th the activity of chronic periodontitis.

Objective:To investigate the levels of IL-8 i n GCF in patients with chronic periodontitis and their relationship with subgingi val Porphyromonas gingivalis(Pg).Methods:10 GCF samples from periodontal healthy volunteers, 60 from the teeth with chronic periodontiti s in patients and 10 from the periodontal healthy teeth in the patients with chr onic periodontitis were collected. Subgingival plaque samples and clinical data on GI, PPD and CAL were obtained. A sensitive ELISA was used to measure IL-8 in GCF and a PCR method was employed to measure the amount of Pg.Resul ts:①Higher total amount of IL-8 in GCF was found in chronic periodonti tis subjects than that in healthy volunteers(P0.05). ②There was positive correlation between the total amount of IL-8 and clinical parameters (GI, PPD, CAL).③There was no correlation between the amount of Pg and the concentration or total amount of IL-8. Conclusions:I L-8 increases with the increace of GCF in the patients with chronic periodontit is, but is not related to Pg in subgingival plaque.

Objective:To study the natural progress of chronic periodontitis and its association with IL-1 genetic polymorphisms.Methods:166 subjects with chronic periodontitis were examined at baseline and in 1 year after 1st visit by measuring the probing depth(PD),attachment loss(AL) and bleeding on probing on 6 sites of each tooth.DNA samples were obtained with buccal swabbing technique and genotyped for IL-1-889 and IL-1+3954 genetic polymorphisms with PCR-RFLP in 111 cases of the subjects.Results:The mean PD,AL increace was 1.12 mm and 1.628 mm respectively within 1 year.249 teeth had more than 2 mm attachment loss over one year period in 94 patients.There was no significant difference for the distribution and frequency of IL-1A-889 genotypes and alleles between the rapidly progressive group(with AL≥2 mm at least one site) and the non-rapidly progressive group.The distribution of IL-1B+3954 genotype 1/2 and the frequency of the allele 2 were significantly higher in the rapidly progressive group than that in the non-rapidly progressive group(P

Objective:To explore whether the recombinant plasmid PcDNA3.1/ sTNFRⅠcould be expressed properly in mice.Methods:The recombinant plasmid PcDNA3.1/sTNFRⅠand the empty vector PcDNA3.1 were directly injected into the right tibialis anterior muscle of each 6 mice respectively. The expression product, sTNFRⅠ, in muscle homogenates was measured by ELISA 7 and 10 days after injection respectively.Results:7 days after injection sTNFR Ⅰ(mg/g) in muscle homogenates in PcDNA3.1/sTNFRⅠ and PcDNA3.1 injected mice was 161.123?4.0 and 69.34?1.489(P

砄bjective:To analyze the DNA polymorphism of Prevotella intermedia(P.i ) and their distribution at diseased and healthy sites in adult patients with periodontitis.Methods:101 P.i strains were obtained from subgingival plaque of the adults with periodontitis.Aabitrarily primed polymerase chain reaction (APPCR)with OPA 03 and OPA 13 as primers was used to study the genetype of P.i .Results:The OPA 03 primer AGTCAGCCAC grouped 101 P.i strains into 7 different genotypes,while OPA 13 CAGCACCCAC divided 97 P.i strains into 12 genotypes.There was mainly one genotype of P.i in each subject,and mainly one genotype of P.i in individual diseased and healthy site.Also,several different genotypes of P.i were found in a identical subject and at a identical site.The predominant genotypes of P.i in diseased sites were mostly the same to that in healthy sites,no special genotype of P.i which was associated to periodontal status was found.Conclusion:Overgrowth of P.i without special genetype may be a pathogen for the pathogenesis of periodontitis.

Objective:To study the relationship between Fc? receptor ⅢB(Fc? RⅢB) genotype and severe chronic periodontitis or generalized aggressive periodontitis.Methods:DNA from 35 subjects with severe chronic periodontitis,25 with generalized aggressive periodontitis and 95 of periodontal healthy controls were sampled by buccal swab and extracted using Chlex-100 method.Polymerase chain reaction with sequence specific primers (PCR-SSP)was carried out to determine the Fc?RIIIB genotype.The data were analyzed with ? 2 test. Results: NA1/NA2 heterozygote was the predominant genotype in the 3 groups.No statistically differences of Fc?RIIIB genotype distribution were found between any two of the stuied groups.The gene frequencies of NA1 and NA2 were 0.6 and 0.4 respectively.Conclusion:No direct relationship exists between Fc?RⅢB genotype and the occurrence of severe chronic periodontitis and generalized aggressive periodontitis according to the present study.