Objective To investigate the clinical efficacy of application of differentiation of traditional Chinese medicine(TCM)syndrome and rectal administration of TCM decoction for treatment of patients with non-small cell lung cancer(NSCLC)and its influence on chemotherapy toxic side effects. Methods Seventy-two patients with NSCLC were randomly divided into two groups:the chemotherapy alone group and rectal administration group(each 36 cases). Both groups received chemotherapy,and the rectal administration group additionally took the rectal administration of self made Kangfei Sanjie decoction(composition:ephedra 6 g,forsythia 20 g,citrus aurantium 20 g,platycodon root 10 g,almonds 10 g,rhubarb charcoal 6 g,scutellariae barbatae 30 g,hedyotic diffusa 30 g,actinidiae 15 g, fritillariae thunbergii 10 g,angelica 10 g,asarum 3 g,mirabilitum depuratum 6 g),and according to the patient's individual situation,the ingredients of the decoction could be added or subtracted to perform pulverization enema, once a day,21 days constituting one therapeutic course. After two cycles of chemotherapy,the changes of tumor lesions,KPS score and the occurrence and degree of chemotherapy adverse reaction were observed. Results The total therapeutic efficiency of rectal administration group was higher than that of chemotherapy alone group,but there was no significant difference between them(63.89% vs. 55.56%,P>0.05);the KPS score of rectal administration group was apparently higher than that of chemotherapy alone group(89.52±10.25 vs. 83.17±8.08,P<0.05). The occurrences ofⅡ-Ⅲdegree adverse reaction after chemotherapy of the rectal administration group was significantly less than those of the chemotherapy alone group:leukopenia(4 cases vs. 11 cases),nausea and vomiting(1 cases vs. 6 cases),fatigue(0 vs. 4 cases),hair loss(3 cases vs. 9 cases),the differences between the two groups being statistically significant(all P<0.05). The influence on liver and renal function was not found in two groups(both P>0.05). Conclusion Application of TCM differentiation of syndrome and rectal administration of TCM drug, Kangfei Sanjie decoction,can significantly improve the quality of life of NSCLC patients,reduce toxic side effects of chemotherapy,and the method is simple and inexpensive.

Objective To observe the effects of ischemic preconditioning of hindlimb (HIP) on the expressions of heat shock protein70 (HSP70) in spinal cord of rats after spinal cord injury (SCI). MethodSprague Dawleys rats were randomly( random number) divided into three groups (20 rats in each group).In sham injury group, rats received laminectomy without SCI; while in the SCI group, rats received traumatic SCI. In HIP group, rats received HIP 8 h before SCI. The SCI animal models were made by using modified Allen's weight-drop device (50 g·cm) on L3-L4 vertebrae. HIP was induced by compressing the two lower limbs of rats alternately with a tourniquet for three cycles of ten-minute ischaemia followed by ten-minute reperfusion. Rats were sacrificed 24 h and 48 h after injury separately. The protein and mRNA of HSP70 of involved spinal cord segments were determined by using RT-PCR, hybridization in situ and immunohistochemistry. Results There was weak expression of HSP70 mRNA in rats of sham injury group, and sporadic positive cells in spinal tissue were found by using immunohistochemistry. The expressions of HSP70 mRNA 24 h and48 hours after SCI in HIP group[(22.18±3.69) and (14.15 ±4.18)] were higher than those in SCI group[(13.97±4.46) and ( 8.73 ± 3.55 )], and the differences were statistically significant (F=16.06, P = 0.005 and F = 7.43, P = 0. 028). The protein levels of HSP70 24 h and 48 hours after SCI in HIP group [(16.71±4.02) and (9.85±2.20)] were higher than those in SCI group [( 14.85 ± 3.73)and (8.78 ±2.05)], and the differences were statistically significant (F =90. 13, P =0.032 and F=34.70, P = 0. 036). Conclusions HIP can increase the transcription and expression of HSP70 in spinal cord of rats following SCI and may have the protective effects on neural tissue.

BACKGROUND:Owing to containing large amounts of growth factors, platelet-rich plasma has been widely used in bone regeneration, wound healing, and so on, while few studies have been reported on cartilage tissue engineering. OBJECTIVE:To explore the effects of platelet-rich plasma on the proliferation and differentiation of chondrocytes, and the feasibility of constructing tissue-engineered cartilage by the combination of chondrocytes and platelet-rich plasma. METHODS:We detected the concentrations of transforming growth factor-β, insulin-like growth factor-1, platelet-derived growth factor and epidermal growth factor BB in the whole blood, platelet-rich plasma, and activated platelet-rich plasma. The rabbit articular chondrocytes were cultured in 10%, 15%, 20%, 30%platelet-rich plasma for 7 days, then the cellproliferation was tested by cellcounting kit-8, and cartilage-related genes (col agen type II, Aggrecan, Sox-9) were determined using QT-PCR. The chondrocytes/platelet-rich plasma composite was implanted subcutaneously into the rabbits, and the samples were harvested after 6 weeks of transplantation for histological examination. RESULTS AND CONCLUSION:The levels of different growth factors in the platelet-rich plasma were higher than those in the whole blood (P<0.05), but lower than those in the activated platelet-rich plasma (P<0.05). Platelet-rich plasma at different concentrations promoted the proliferation of chondrocytes. When the concentration of platelet-rich plasma was no more than 20%, the proliferation of chondrocytes showed a concentration-dependent manner. The 20%platelet-rich plasma showed the best effects to promote the expression of col agen type II (P<0.05), while the 15%platelet-rich plasma could maximize the expression of Sox-9 and Aggrecan (P<0.05). After transplantation of chondrocytes/platelet-rich plasma composite, cartilage-like tissue formed with cartilage lacuna-like structures, and rich extracellular matrix was found, which indicates platelet-rich plasma can be used as an injective scaffold in cartilage tissue engineering.

Objective This study was designed to evaluate the biomechanical properties in tendons across the joints when Palmar or dorsal stitches were used. Methods Twenty porcine tendons were transected and then repaired in zone Ⅱand divided into two groups,using modified Kessler by placing the stitches in volar or dorsal part of tendons.The repaired tendons were placed on a joint model with flexion of 60?. An instron tensile testing machine was used to investigate 2 mm gap formation force and ultimate tensile strength. Results 2 mm gap formation force and ultimate tensile strength were both higher when dorsal placement of stitches was used. Conclusions Dorsal placement of stitches was recommended for repairing the tendons across the joints because it can offer greater tensile strength.

Objective To present a retrospective analysis of the clinical outcome in patients treated by local intra-arterial thrombolysis (LITT). Using urokinase for acute ischemic stroke of the anterior or posterior arterial circulation.Methods Twenty-six patients were treated with LITT using urokinase. The effects all patients were assessed by using angiography, brain CT and stroke scale before and after treatment. Results Among 26 patients, the complete reperfusion of blood vessel appeared in 7 and partial reperfusion in 11.The efficiency rate reached 78%.Conclusions Cerebral arterial thrombolysis with UK can improve neurologic function defect in 84% of patients with acute ischemic stroke after 15 days, suggesting one of the best ways for treatment.

Objective To investigate the role of p53, rasp21 and C-myc in gastric carcinogenesis. Methods Expression and distribution feature of p53, rasp21 and C-myc in 60 cases of human gastric cancer were detected with immunohistochemical SP (Streptavidin-Peroxidase) staining. Results The positive rate of expression was respectively 51.7% for p53, 40.0% for rasp21, and 66.7% for C-myc, while in the gastric mucous membrane adjacent to carcinoma they were respectively 1.6% for p53, 13.3% for rasp21, and 15.0% for C-myc. The differences in expression of p53, rasp21 and C-myc oncoproteins in carcinoma and non-carcinoma tissues were very significant (P

Objective To evaluate the effects of 4 different epitendinous sutures on the tensile strength of the repaired tendons. Methods 40 plantar flexion deep tendons from pigs were divided into 4 groups randomly, with 10 in each group. After the core suture of Modifier Kessler was applied, 4 epitendinous sutures at different distances from suture sites to broken ends (4mm, 7mm, 10mm, 12mm) were used respectively in each group to repair the transected tendons. MTS Instron 4411 was used to test the repaired tendons. Tensile strengths at 2mm gap formation and at the peak were collected for statistical analyses by ANOVA. Results In this test, the tensile strength was found to increase with the distance from suture sites to broken ends. The tensile strength in the 4mm group was significantly lower than that in the others. There was little statistical difference between the other 3 groups. Conclusion Tensile strength of the repaired tendon tends to increase with the distance from suture sites to broken ends in epitendinous suture.

Objective To investigate the effects of early continuous renal replacement thempy(CRRT)on correlation kidney injury and prognosis in patients with severe acute pancreatitis(SAP).Methods According to the digital table,40 SAP patients were randomly divided into two groups:the control group(21 cases)and CRRT treat-ment group(19 cases).The levels of serum creatinine,urea nitrogen,IL -1,IL -6,TNF -α,the APACHEⅡscore, the incidence of mechanical ventilation were compared between the two groups.Results The levels of serum creati-nine,urea nitrogen were significantly lower in the CRRT group than those in the control group in day 3(t =2.836, 2.952,P =0.003,0.004);The levers of IL -1,IL -6,TNF -αwere significantly lower in the CRRT group than those in the control group in day 3(t =2.376,2.414,2.197,P =0.351,0.028,0.042);The APACHE II score,inci-dence of mechanical ventilation,the fatality rate in the CRRT group were significantly lower than those in the control group in day 3[(20.16 ±5.23)points vs.(13.83 ±4.48)points,14 cases(66.7%)vs.6 cases(31.6%),8 cases (38.1%)vs.2 cases(14.3%),t =4.572,χ2 =4.912,4.043;P =0.0329,0.027,0.044].Conclusion Early CRRT therapy can eliminate the IL -1,IL -6 and TNF -αin SAP patients,can reduce the incidences of AKI,which may provide more clinical benefits in the early phase of SAP.

In order to understand the alcohol's toxicity to the quantitative alternations of synapses in mouse visual cortex, the expression of synaptophysin after prenatal alcohol exposure was investigated. In present study, the experimental mice at P0, P7, P14 and P30 were grouped, as control, 2 g x kg(-1) alcohol treatment and 4 g x kg(-1) alcohol treatment. The pre-synaptic elements which were used to represent synapses were marked with synaptophysin (a synaptic vesicle associated protein) by immunocytochemistry technique. The synaptophysin positive boutons in layer VI of visual cortex were imaged under laser confocal microscope. With stereological methods, the number cal density of synapse in visual cortex was calculated in different groups at various ages. Moreover, Western blotting was carried out to detect the expression of synaptophysin in visual cortex. The results showed that prenatal alcohol exposure could cause synaptic loss with long-term effect and in a dose dependent manner. For instance, there were significant difference among the different treatment groups of P0, P14 and P30 as well (P < 0.05). Western blotting supported the results of immunofluorescent labeling. In conclusion, prenatal alcohol exposure can induce the synaptic loss dose dependently and with long-term effect. Our findings implicate that the synaptic loss with long-term effect in CNS probably contributes to the lifelong mental retardation and memorial lowliness associated with childhood FAS.