Objective Through the statistical analysis of the student assessment on curriculum quality,through the observation of the current curriculum teaching situation and its cheracteristics to promote the curriculum construction effectively and improve the medical teaching quality.Methods The current study is carried out among 2 841 undergraduates who study in varied majors and different grades in a medical university.A table of Student Evaluation of Course Teaching Quality developed by the researcher is applied to the questionnaire survey and statistical analysis including comparation means test and correlation tests are adopted in the present study to analyze the course teaching quality.Results Initially.the National Selected Model Courses show no significant difference from the common courses;secondly,three variables including teaching content adopting the course syllabus,good professional qualification and teaching capability of teachers and advanced teaching methods and techniques ale highly scored among ten variables;thirdly,the correlation coefficient of the variable of improving self-learning ability and total score is statistically significant and it can be observed higher than that of other variables and total score.Conclusion Establishing student-centered curriculum evaluation and construction of long-effect mechanism,and developing the students'ability of autonomous learning ale important to improve the quality ofthe course.

The indexes of teaching quality evaluation,such as attitude,teaching and evaluation,contents and methods,and evaluation standards,such as "excellent,good,medium,bad,worse",show the characteristics of inaccuracy.While traditional evaluation methods focus on accurate figures,the paper proposes a new method which is based on fuzzy mathematics to evaluate teaching quality.The theory,computing procedure and examples of the method are introduced.

The methods and experiences of anatomy,histology and embryology teaching in English for foreign students are discussed in this article to exchange experiences with each other and progress together.

Objective To establish a simple ,rapid,highly specific and sensitive molecular detection of carbapenem-resistance acinetobacter baumannii(CRAB)OXA-23 genes,and this method is used to detect the multiple drug-resistant acinetobacter bau-mannii in our hospital,and the purpose is to know the antibiotic resistance of CRAB OXA-23 genes .Methods The loop-mediated isothermal amplification(LAMP)was established for detection of the CRAB OXA-23 genes,and a set of specific primers were de-signed by special software,PrimerExplorer version 4.The LAMP assay was developed on using SYBR Green Ⅰ for fluorescent chromogenic reaction substances,improved through a series of optimization tests,and through macroscopic observation and electro-phoresis test comparison results.At the same time,the application of LAMP was used to test 41 multiple drug-resistant acineto-bacter baumanniis which were collected from December 2013 to March 2014 in our hospitalized patients.Results The ladder ban-ding was produced in CRAB OXA-23 genes strains by the LAMP detection through electrophoresis test,however,no ladder ban-ding was observed in the others .The color of the amplification product in genes strain CRAB OXA-23 changed from orange to green by adding 1 μL SYBR Green Ⅰ,however it was still orange in others.The sensitivity of the LAMP detection in pure cultrue was 5 cfu/μL of the CRAB OXA-23 genes cells.Application of LAMP was used to separate multiple drug-resistant acinetobacter baumanniis from hospitalized patients ,32 strains were tested in 41 strains,the positive rate was 78.04%.Conclusion Separation of the CRAB OXA-23 genes carry rate is higher in our hospital ,and they have very high resistance of commonly used antibacterial drugs.The LAMP method to test OXA-23 gene of CRAB was established in this research was simple ,fast,sensitive and specific. Therefore,it is especially suitable wider use at the grass-roots unit,and it is of great significance for selecting reasonable choice of antibiotics by clinical doctor.

Objective To establish a novel real-time PCR method to detect HCV RNA using Selfreporting duplex mutation primers.Methods The recombinant vector pMD18-T-HCV 5′-NCR was used as the calibrator.The Self-reporting duplex mutation primers were designed according to the gene sequence.And then the PCR reaction system was optimized and evaluated.The specificity,sensitivity and reproducibility of real-time PCR were estimated,The serum specimens from 90 cases(30 cases of HCV,30 cases of other viral hepatitis and 30 healthy volunteers) were tested with this real-time PCR; Results were compared with those obtained using a commercial TaqMan kit.Results The assay was established.It showed linearity over a wide range from 20 - 109 IU/ml.Intra-experimental coefficients of variation(CVs) were 1.37% -4.59%,and inter-experimental CVs were 1.58% -4.81%,respectively.There was no significant difference of HCV genome number tested by the two methods(R2 = 0.95) in 30 hepatitis C patients; HCV DNA was not detected in any serum samples of 30 healthy volunteers by the two methods.The specificity was 100%(60/60).All the samples in patients with clinically confirmed HCV infections showed HCV RNA positive.There wass good correlation between the quantitaive results and results obtained using the commercial TaqMan kit.Conclusions It is demonstrated that real-time PCR is a reliable,accurate and feasible assay for HCV.The establishment of this assay provided alternative technology for clinical diagnosis or therapeutic drug monitoring in the field of HCV infection and epidemiologic survey.

In order to understand the alcohol's toxicity to the quantitative alternations of synapses in mouse visual cortex, the expression of synaptophysin after prenatal alcohol exposure was investigated. In present study, the experimental mice at P0, P7, P14 and P30 were grouped, as control, 2 g x kg(-1) alcohol treatment and 4 g x kg(-1) alcohol treatment. The pre-synaptic elements which were used to represent synapses were marked with synaptophysin (a synaptic vesicle associated protein) by immunocytochemistry technique. The synaptophysin positive boutons in layer VI of visual cortex were imaged under laser confocal microscope. With stereological methods, the number cal density of synapse in visual cortex was calculated in different groups at various ages. Moreover, Western blotting was carried out to detect the expression of synaptophysin in visual cortex. The results showed that prenatal alcohol exposure could cause synaptic loss with long-term effect and in a dose dependent manner. For instance, there were significant difference among the different treatment groups of P0, P14 and P30 as well (P < 0.05). Western blotting supported the results of immunofluorescent labeling. In conclusion, prenatal alcohol exposure can induce the synaptic loss dose dependently and with long-term effect. Our findings implicate that the synaptic loss with long-term effect in CNS probably contributes to the lifelong mental retardation and memorial lowliness associated with childhood FAS.

The influence of temperature, chloride ions and sulfide ions on the anticorrosion behavior of 316L stainless steel in simulated cooling water was studied by electrochemical impedance spectroscopy and anodic polarization curves. The results show that the film resistance increases with the solution temperature but decreases after 8 days' immersion, which indicates that the film formed at higher temperature has inferior anticorrosion behavior; Chloride ions and sulfide ions have remarkable effects on the electrochemical property of 316L stainless steel in simulated cooling water and the pitting potential declines with the concentration of chloride ions; the passivation current has no obvious effect; the rise of the concentration of sulfide ions obviously increases the passivation current, but the pitting potential changes little, which indicates that the two types of ions may have different effects on destructing passive film of stainless steel. The critical concentration of chloride ions causing anodic potential curve's change in simulated cooling water is 250 mg/L for 316 L stainless. The effect of sulfide ions on the corrosion resistance behavior of stainless steel is increasing the passivation current density Ip. The addition of 6mg/L sulfide ions to the solution makes Ip of 316 L increase by 0.5 times.

With the development of the society and economy,the change of the medical model and the improvement of the medical technology jointly raise new requirements on medical talents.In reply to these demands,this paper elaborates systemically the meaning of the health-correlated profession,the neccessity of its development,the way to educate it and its future.

Objective To investigate the effect, preponderance and clinical significance of the examination of gastrointestinal transmitting function with small amount of barium meal. Methods Thirty cases of healthy subjects and 50 patients with constipation swallow 40 g barium meal once, then abdominal fluoroscopy or plain films were taken until barium meal being egested totally. Results The transit time of stomach, small intestine, colon, rectum and the whole digestive tract were (189?64)minutes, (330?108)minutes, (2239?659)minutes, (136?49)minutes, (2801?735)minutes in healthy subjects and (212?87)minutes, (366?94)minutes, (3665?1322)minutes, (650?219)minutes, (5973?2341)minutes in constipation patients respectively. The transit time of colon and rectum in constipation patients was obviously longer than that of healthy subjects (P0.05). Conclusion The pictures of the examination with small amount barium meal are clear, precise, reliable, and provide exact data for constipation classification.

The prenatal ethanol exposure induced the alterations of dendritic spine and synapse in visual cortex and their long-term effect would be investigated in mice from P0 to P30. Pregnant mice were intubated ethanol daily from E5 through the pup's birth to establish mode of prenatal alcohol abuse. The dendritic spines of pyramidal cells in visual cortex of pups were labeled with DiI diolistic assay, and the synaptic ultrastructure was observed under transmission electron microscope. Prenatal alcohol exposure was associated with a significant decrease in the number of dendritic spines of pyramidal neurons in the visual cortex and an increase in their mean length; ultrastructural changes were also observed, with decreased numbers of synaptic vesicles, narrowing of the synaptic cleft and thickening of the postsynaptic density compared to controls. Prenatal alcohol exposure is associated with long-term changes in dendritic spines and synaptic ultrastructure. The changes were dose-dependent with long term effect even at postnatal 30.