Objective To investigate the drug resistance characteristics of Klebsiella pneumoniae lower respiratory tract infection in the patients with coexisting pulmonary tuberculosis (TB) and diabetes mellitus (DM ) to provide a basis for the prevention and treatment of Klebsiella pneumoniae infections .Methods The lower respiratory tract specimens were collected from the patients with coexisting pulmonary TB and DM in the Shenzhen Hospital of Peking University from January 2009 to May 2015 .The bacteri‐al species was identified by using the France Bio‐Merieux Vitek2‐Compact automatic microorganism identification instrument ,the drug susceptibility tests adopted the disk method (K‐B) ,and extended spectrum β lactamase (ESBLs) detection was conducted ,the drug susceptibility test results were judged according to the CLSI standards .The data were analyzed by the WHONET 5 .6 and SPSS18 .0 software .Results 139 strains of Klebsiella pneumoniae were isolated from the lower respiratory tract specimens in the patients with coexisting pulmonary TB and DM ,the ESBLs producing rate was up to 46 .0% .The drug susceptibility testing results showed that the resistance of non‐ESBLs producing and ESBLs producing Klebsiella pneumoniae to cefoperazone / sulbactam ,pip‐eracillin/tazobactam ,imipenem ,meropenem ,cefotetan and amikacin was lower and less than 15 .0% ,the resistance of ESBLs‐produ‐cing strains to most antibacterial drugs was significantly higher than that of non‐ESBLs producing strains (P< 0 .05) .Conclusion Klebsiella pneumoniae isolated from the patients with coexisting pulmonary TB and DM complicating lower respiratory tract infec ‐tion in this hospital has the high detection rate of ESBLs producing strains and strong drug resistance ,therefore clinic should strengthen the ESBLs detection and drug resistance monitoring for preventing the generation of multi‐drug resistance .In the treat‐ment of its infection ,the first choice is containing enzyme inhibitors and carbapenems antibacterial drugs .

Objective To investigate the diagnostic value of combined detection of neutrophil CD64 index and C reactive protein (CRP) levels in newborn bacterial infectious diseases .Methods The retrospective analysis was performed on 139 neonates admitted to the neonatal department of our hospital from January to December 2015 ,including 72 cases in the bacterial infection group and 67 cases in the viral infection group ,and 50 healthy neonates were selected as the control group .The levels of CD64 and CRP and the positive rates were compared among 3 groups and analyzed .The sensitivity ,specificity ,positive predictive value and negative predictive value of CD64 and CRP in the diagnosis of bacterial infection were analyzed .Results The CD64 index and CRP levels had statistical difference between the bacterial infection with the viral infection group and normal control group (P<0 .05) ,the CD64 index and CRP levels after treatment in the bacterial infection group were significantly decreased ,the differences were statistieally significant(P<0 .05) .The sensitivity ,specificity ,positive predictive value and negative predictive value of CD64 index in the diag‐nosis of bacterial infection were 91 .7% ,92 .5% ,93 .0% and 91 .2% respectively ,which were significantly higher than those of CRP ,the differences were statistically significant (P< 0 .05).CD64 index was positively correlated with CRP (r= 0 .781 ,P<0 .01) .Conclusion The combined detection of CD64 index and CRP level helps to early diagnosis ,differential diagnosis and the therapeutic effect evaluation of bacterial infection in neonates ,moreover the diagnostic efficiency of CD64 is obviously better than that of CRP .

Objective To explore the application value of serum procalcitonin (PCT ) and amyloid protein A (SAA ) in the early diagnosis and curative evaluation of bacterial infectious diseases among preschool children in order to improve the clinical diagnosis level of early bacterial infection .Methods A total of 120 cases of pediatric infectious diseases in our hospital from January to De‐cember 2015 were selected and divided into the bacterial infection group (60 cases) and viral infection group (60 cases) ,meanwhile 50 children undergoing the physical examination were selected as the control group .The serum levels of PCT and SAA were ob‐served and compared among the three groups ,and the statistical analysis was performed by using the SPSS 19 .0 software .Results The levels of PCT and SAA in the bacterial infection group were significantly increased compared with the viral infection group and the healthy control group(P<0 .05) ,both PCT and SAA levels after effective antibiotic treatment were significantly decreased (P<0 .05);the positive rates of PCT and SAA in the bacterial infection group were 91 .7% and 95 .0% respectively ,which were signifi‐cantly higher than those in the viral infection group and the healthy control group (P<0 .05) ,the sensitivity of PCT in the diagno‐sis of bacterial infection was lower than that of SAA ,but the specificity ,positive predictive value and negative predictive value were all higher than those of SAA ,the correlation analysis showed that PCT level in the bacterial infection group was positively correla‐ted with and the SAA level(r=0 .782 ,P<0 .01) .Conclusion Detecting the PCT and SAA levels is conducive to early diagnosis , judgment of the disease condition and guidance of rational medication in bacterial infection among preschool children ,in which the efficiency of PCT for diagnosing bacterial infection is superior to SAA .

Objective To detect hs‐cTnT and cTnI for exploring their application value in the early diagnosis of acute myocardial infarction(AMI) .Methods From October 2015 to March 2016 ,100 patients with AMI in the cardiology department of our hospital were selected as the observation group(AMI group) and contemporaneous 100 adults undergoing physical examination as the con‐trol group .The chemiluminescence immunoassay was used to detect the levels of hs‐cTnT and cTnI .The positive rate ,sensitivity and specificity of the hs‐cTnT and cTnI in the AMI group for early diagnosis of AMI were compared .Results The level of hs‐cT‐nT in the AMI group was (4 .89 ± 1 .83)ng/mL ,which was higher than that in the healthy population by 99 percentile value 0 .014 ng/mL ;the cTnI level in the AMI group was (28 .82 ± 12 .32)ng/mL ,which was higher than that in the healthy population by the upper limit of normal reference value 0 .4 ng/mL ,both of them were significantly higher than those in the control group ( P<0 .05) ,the positive rate and sensitivity of hs‐cTnT was both 92 .0% ,which was significantly higher than 79 .0% of cTnI both(P<0 .05) ,the specificity of cTnI was 96 .0% ,which was significantly higher than 76 .0% of hs‐cTnT(P<0 .05) .Conclusion The pos‐itive rate and the sensitivity of hs‐cTnT are high ,the specificity of cTnI is high ,their joint detection has an important significance for the early diagnosis of AMI .

We analyzed the whole genome coding sequence of Volvariella volvacea to study the pattern utilization of codons by Codon W 1.4.2. As results, 24 optimal codons were identified. Moreover, the frequency of codons usage was calculated by CUSP program. We compared the frequency of codons usage of V. volvacea with other organisms including 6 modal value species (Homo sapiens, Saccharomys cerevisiae, Arabidopsis thalian, Mus musculus, Danio rerio and Drosophila melanogaster) and 4 edible fungi (Coprinopsis cinerea, Agaricus bisporus, Lentinula edodes and Pleurotus ostreatus). We found that there were less differences in 3 edible fungi (excluding Pleurotus ostreatus) than 6 modal value species, comparing with the frequency of codons usage of V. volvacea. With software SPSS16.0, cluster analysis which showed differences in the size of codon bias, reflects the evolutionary relationships between species, which can be used as a reference of evolutionary relationships of species. This was the first time for analysis the codon preference among the whole coding sequences of edible fungi, serving as theoretical basis to apply genetic engineering of V. volvacea.
Agaricales ; genetics ; Animals ; Arabidopsis ; genetics ; Cluster Analysis ; Codon ; DNA, Fungal ; genetics ; Drosophila melanogaster ; genetics ; Humans ; Mice ; Saccharomyces cerevisiae ; genetics ; Software ; Volvariella ; genetics ; Zebrafish ; genetics

Purpose: This study aimed to develop a nomogram for predicting pathologic complete response (pCR) after neoadjuvant chemoradiotherapy (CRT) in patients with esophageal squamous cell carcinoma (ESCC) by integrating hematological biomarkers and clinicopathological characteristics. Materials and Methods: Between 2003 and 2017, 306 ESCC patients who underwent neoadjuvant CRT followed by esophagectomy were analyzed. Besides clinicopathological factors, hematological parameters before, during, and after CRT were collected. Univariate and multivariate logistic regression analyses were performed to identify predictive factors for pCR. A nomogram model was built and internally validated. Results: Absolute lymphocyte count (ALC), lymphocyte to monocyte ratio, albumin, hemoglobin, white blood cell, neutrophil, and platelet count generally declined, whereas neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) increased significantly following neoadjuvant CRT. After surgery, 124 patients (40.5%) achieved a pCR. The pCR group demonstrated significantly more favorable survival than the non-pCR group. On multivariate analysis, significant factors associated with pCR included sex, chemotherapy regimen, post-CRT endoscopic finding, pre-CRT NLR, ALC nadir during CRT, and post-CRT PLR, which were incorporated into the prediction model. The nomogram indicated good accuracy in predicting pCR, with a C-index of 0.75 (95% confidence interval, 0.71 to 0.78). Conclusion Female, chemotherapy regimen of cisplatin/vinorelbine, negative post-CRT endoscopic finding, pre-CRT NLR (≤ 2.1), ALC nadir during CRT (> 0.35 ×109/L), and post-CRT PLR (≤ 83.0) were significantly associated with pCR in ESCC patients treated with neoadjuvant CRT. A nomogram incorporating hematological biomarkers to predict pCR was developed and internally validated, showing good predictive performance.

ObjectiveTo explore the effect of Qingre Huayu Jianpi prescription （QHJ） on colitis-associated colorectal cancer （CAC） in mice， and its related mechanism. MethodC57BL/6 mice were randomly divided into four groups including the normal， model， QHJ low-dose （QHJ-L， 10 g·kg^-1）， and QHJ high-dose （QHJ-H， 40 g·kg^-1） groups. Azoxymethane （AOM） and dextran sodium sulfate （DSS） were combined to chemically build a CAC mouse model for 14 weeks. Each drug group was given intragastrically from the 5^th week to the 14^th week， once per day. An equal volume of water was fed to the normal and model groups. The mouse survival rate， colon length， weight， and pathological alterations were assessed. The protein expressions of Wnt-3a protein signaling （Wnt3a）， β-catenin， Non-phosphor-β-catenin （Non-p-β-catenin）， and cholesterol-binding glycoproteins 133 （CD133） were detected by Western blot. The localization and expression of the cluster of differentiation （CD） 80 and CD11 antigen-like family member B （CD11b） were detected by immunohistochemistry （IHC）. The colon organoids derived from CAC mice were isolated and cultured to detect the expression of Wnt signaling pathway-related proteins. ResultThe survival rate of the CAC mice was improved by QHJ treatment and the number of colon tumors was inhibited significantly. Compared with those in the normal group， the expression levels of Wnt3a， β-catenin， Non-p-β-catenin， and CD133 in colon tissues in the model group were significantly increased （P<0.05， P<0.01）. Compared with those in the model group， the levels of Wnt3a and β-catenin in the QHJ-L group were significantly decreased （P<0.01）， and the protein levels of Wnt3a， β-catenin， Non-p-β-catenin， and CD133 in the QHJ-H group were significantly decreased （P<0.05， P<0.01）. Meanwhile， the expression level of CD11b in the model group was significantly increased compared with that in the normal group while the CD80 level was significantly decreased （P<0.05， P<0.01）. Compared with those in the model group， CD11b in QHJ-L and QHJ-H groups was significantly decreased， and CD80 was significantly increased（P<0.05， P<0.01）. The expressions of Non-p-β-catenin and CD133 in colonic organoids of CAC model mice were significantly increased， while QHJ treatment could inhibit the expressions of Non-p-β-catenin and CD133 in colonic organoids （P<0.01）. ConclusionQHJ could inhibit the inflammation-cancer development in CAC mice， the mechanism of which might be related to regulating the microenvironment and inhibiting the over-activation of Wnt signaling.