Objective To explore the effects of Tangzhiping granules on the blood glucose and IGF-Ⅰ in T2DM rats.Methods 40 SPF grade Wistar rats whose weight were about (200±20)g were selected as the subjects for this study,one half were male and another half were female.After be adapted to the normal food for 1 week,8 rats were selected randomly from all of rats following their weight as the blank group (ordinary feed).The remaining the others were fed with the high-fat and high-sugar food.The 6th week,the other rats would be undergo intraperitoneal injection once in left lower belly with 40 mg/kg streptozotocin (STZ),and enable them to become T2DM rats.The 8th week,we would select successful animal models 32 which were divided randomly into a modle group,a Tangmaikang (TMK) granules group,a Tangzhiping granules (TZP)group.The 12th week,we would execute all of rats and collect the blood serums which were tested for the blood glucose and IGF-Ⅰ.Results () Compared model group with blank group,the levels of the blood glucose of the model group increased to (28.33±0.50)mmol/L,while the IGF-Ⅰ decreased to (3.40 ± 0.83) pg/ml,which showed the model of type 2 diabetic rats was successfully set up.② The levels of the blood glucose of the Tangzhiping granules group and Tangmaikang granules group were lower than that of the model group to (15.06±0.39)mm ol/L,(20.12±0.58)mmol/L,but the levels of the IGF-Ⅰ were higher to (9.39±2.91)pg/ml,(5.58± 1.09)pg/ml,which implied that each group of pharmacological treatment had a good effect.③ The level of the blood glucose and IGF-Ⅰ of the Tangzhiping granules group was also significantly different from that of the Tangmaikang granule group,the therapeutic effect of Tangzhiping granules group was better than that of Tangmaikang granules group.Conclusion Tangzhiping granules can reduce the blood glucose of T2DM rats.And also can increase IGF-Ⅰ of T2DM rats.Tangzhiping granules can improve the various symptoms of diabetes mellitus at the same time.

Objective To determine the impact of PEE with different ethanol concentration extract on the activity of tyrosinase in B16 melanoma and to explore the feasibility of PEE used as skin whitening agent make use of a model organism .Methods 30%, 50%, 70%, 95%, different concentration of ethanol reflux got four kinds of PEE alcohol extract, respectively.And selected the most active inhibition to mushroom tyrosinase ( PEE30、PEE50、PEE70、PEE95).The effect of extracts from the selected on B16 cell model was determined by MTS, NaOH splitting decomposition, L-DOPA oxidation progress.The change in protein expression level after ethanol extract of PEE was determined by Western bolt.Results The capacity of inhibition to mushroom tyrosinase in each concentration of PEE extraction, but PEE70 had the most active inhibition.When challenged with B16 cell model, PEE70 showed the capacity of decrease in tyrosinase activities and melanin synthesis (P<0.01) and had a does dependence ;it also decreased Tyrosinase and TRP-1 expression compared with control B16 cells.Conclusion These data support the idea that PEE can restrain melanogenesis, through its inhibitory effect on the activity of tyrosinase.