Objective To investigate the relation of DNA content and SPF to the clinicopathological characteristic in lung cancer.Methods Fresh specimens taken from 56 patients with lung cancer and 36 patients with non-malignant pulmonary lesions were measured for DNA index(DI),S-phase fraction(SPF) by using FACSCalibur 4200 flow cytometry.Results ① DNA index(DI) of lung cancer was 1.18?0.33,0.99?0.07 in lung cancer and non-malignant groups,respectively.The percentage of heteroploid was 78.6% in lung cancer,(5.6%) in non-malignant.DI and the positive rate of heteroploid were significantly higher in lung cancer groups than that in non-malignant group(P0.05);③ It was demonstrated that SPF was significant higher in lung cancer groups than that in non-malignant group. The SPF of heteroploid tumors was higher than that of diploid tumors(P

Objective To analyze the drug resistance of Mycobacterium tuberculosis (MT) in elderly patients with recurrent pulmonary tuberculosis .Methods We collected 1 860 M T strains from elderly patients with recurrent pulmonary tuberculosis in our hospital from January 2005 to December 2013 to review the drug resistance profile of these strains .Another 300 patients with naive pulmonary tuberculosis were included as control group .Results Significant difference was found between the males and females in terms of the resistance rate to streptomycin ,rifampicin ,ethambutol ,amikacin ,capreomycin ,or ofloxacin (P＜0 .05) .Multi‐drug resistant tuberculosis (MDR‐TB) was identified in 24 .0% (357/1 489) and extensively drug‐resistant tuberculosis (XDR‐TB) in 9 .8% (146/1 489) in male patients ,which were significantly lower than the corresponding rates of 34 .2% (127/371) and 19 .7% (73/371) in the female patients (P＜0 .05) .In all the 1 860 patients with recurrent pulmonary tuberculosis , MDR‐TB and XDR‐TB accounted for 26 .0% and 11 .8% , respectively , significantly higher than the corresponding rates of 11 .3% (34/300) and 7 .0% (21/300) in the control patients with naive pulmonary tuberculosis .Single drug resistance was identified in 20 .0% (372/1 860) of the patients with recurrent tuberculosis and 41 .0% (123/300) of the control patients (P＜0 .05) .Conclusions The MT strains isolated from elderly patients with recurrent pulmonary tuberculosis show higher resistance rate ,especially in females .It is necessary to strengthen the monitoring of drug resistance in old patients with recurrent pulmonary tuberculosis .

[Aim]To create CPI mode of teaching Chinese as a foreign language in a TCM university. [Method]Based on conventional teaching, language partner and interculture communication class(mixing Chinese and international students) were introduced in CPI mode. The effect of this mode was ana-lyzed. [Result] By effectively promoting the cooperative learning between Chinese and international students, CPI mode could not only improve Chinese teaching effect, but also improve international students’ability on intercultural communication.[Conclusion] Based on its practice, CPI mode is believed as an effective way to improve teaching and study.

Objective To cvaluate the efficacy and safety of HabibTM VesOpen intravascular radiofrequency catheter percutaneous radiofrequency ablation combined with TACE in the treatment of portal vein tumor thrombus of primary hepatocellular carcinoma.Methods Thirty-nine patients with primary hepatocellular carcinoma complicated with portal vein thrombosis were treated with HabibTM VesOpen intracavitary catheter ablation combined with TACE.The postoperative success rate,complication,blood index,α-fetoprotein (AFP),portal vein patency and tumor thrombus imaging changes were assessed.Results All of 39 patients underwent surgery successfully without occurrence of perforation,infection,hepatapostema,intra-abdominal hemorrhage nor other complications associated with surgery.Blood routine examinations (WBC,RBC,PLT) after 2 weeks and 4 weeks of surgery had no statistical difference compared with those of preoperation.Liver function indexes including ALT,AST,ALB had statistical difference before and after the surgery except for TBIL.AFP had statistical difference before and after surgery.Doppler ultrasonography after 4 weeks showed blood flow in original blocked portal vein.Enhanced CT or MR examinations after 8 weeks on the abdomen,suggested that tumors were shrinked or disappeared.Conclusion Radiofrequency catheter ablation with intracavitary radiofrequency catheter of HabibTM VesOpen is an effective method in the treatment of portal vein tumor thrombus in primary hepatocellular carcinoma.

Aseptic loosening of the prosthesis due to periprosthetic osteolysis is one of the main causes that affect the service life following artificial joint replacement. The patient should undergo revision of artificial joints,but the surgery is of high difficulty,high risk and excessive cost,etc. Therefore,using nonsurgical technique to control artificial prosthesis osteolysis is an urgent and realistic task. Currently,the drug prevents osteolysis through inhibiting osteoclast bone resorption,responding to cytokines,and promoting bone formation. In addition,gene therapy and other treatments for osteolysis are under exploration.

[Objective]To observe the change of ultrapathology in the rat model study of experimental lumbar intervertebral disc degeneration.[Method]Thirty-two Sprague-Dawley(SD)rats were divided into two guoups at random,including in the experimental group and the control group.The experimental group was incised through posterior approach along spinous process,resected below erector spinal muscle,the supraspinal and interspinal ligament and the posterior of the zygapophysial joint at the center of the third lumbar spine for providing the rat degeneration of lumbar intervertebral induced by mechanical instability state.The control group was just incised the skin.All of th rats were examined after eight weeks by electronmicroscope.[Result]There was no special ultrapathological characterization after operation in the control group,while in experimented group most chonduocyte-like cells were degenerated or necrotic and their number decreased markedly,collagenous fibrils appeared various kinds of degeneration including fusion,twist and calcification,and there were gaps between the collagen bundles.[Conclusion]Observing the change in the rat model study of experimental lumbar intervertebral disc degeneration by ultrapathology,it can offer the experimental data studying tile degeneration of lumbar intervertebral disc.

Objective To explore the role of RBC[Ca2+]i levels in pathogenesis of hypoxic-ischemic encephalopathy (HIE) in neonates. Methods Twenty-eight neonates with moderate and severe HIE hospitalizeal from Jun. 2002 to Mar. 2006 were enrolled the study. The neonates with HIE were given routine treatment and Nimodipine for 7～10 days. Blood samples were collected before treatment and at 72 hours,7～10 days after treatment respectively. The levels of RBC [Ca2+]i were measured by Fura-2/AM. Twenty healthy full-term neonates were studied as controls. Results (1) The levels of RBC [Ca2+] i in the neonates with moderate and severe HIE were significantly higher than that in control group at every time points( P＜0. 05 ,P＜0.01). (2) the levels of RBC[Ca2+]i in the neonates with moderate and severe HIE peaked at 72 hours after treatment,and were still significantly higher than that of control group at 7～10 days after treatment(P＜0. 05). (3) In the neonates with HIE,RBC[Ca2+ ]i levels correlated positively with the severity of HIE ( r = 0. 447, P＜ 0. 05 ). Conclusion RBC [Ca2+ ] i levels are closely associated with pathogenesis of HIE, and may play an important role in the pathogenesis of HIE. Evaluating RBC [ Ca2+] i levels in neonate after birth may provide clinical clues for the early diagnosis and prognosis assessment of HIE.

OBJECTIVE To study fungal isolation and its drug resistance from abacterial body fluid.METHODS The abacterial body fluid for patients in our hospital was cultured by BacT Alert 120 and routine method.The strains which had been isolated were identified by development culture method and VITEK-YBC card or API-20C AUS reagent stripes.Furthermore,drug sensitive test was done by ATB FUNGUS2 drug sensitive reagent kit.RESULTS From the 898 samples with positive cultures of abacterial body fluid.8 species and 79 strains were isolated.Among the 79 fungal strains(8.8%),Candida albicans was 40 strains(50.6%),C.tropicalis was 12 strains(15.2%),C.glabrata was 11 strains(13.9%),C.parapsilosis was 9 strains(11.3%),C.guilliermondii was 4 strains(5.1%),C.krusei was 1 strain(1.3%),Cryptococcus were 1 strain(1.3%),and Mucor were 1 strain(1.3%).From them,13 strains(16.5%) were isolated in ICU,10 strains(12.7%) were in neurosurgical department,9 strains(11.4%) in oncological department,8 strains(10.1%) in urological surgery department,7 strains(8.9%) in thoracic surgery department,7 strains(8.9%) in pneumology department,6 strains(7.6%) in hematology department,5 strains(6.3%) in gerontology department,4 strains(5.1%) in orthopedic department,4 strains(5.1%) in endocrinology department,2 strains(2.5%) in nephrology department,and 4 strains(5.1%) in other units.The antifungal activity of amphotericin B,nystatin and flucytosine for fungus was quite perfect with sensitive ratio of 90%.But the drug resistance ratio to miconazole,ketoconazole and econazole was upward on 20-30%.CONCLUSIONS It′s important and necessary to monitor the circumstance of fungal infection and resistance of the pathogenic fungi due to its increased morbidity.

Objective: The pilot study was to explore the possibility of discriminating schizophrenia from the normal with combination of multiple tests. Methods: Forty medication-naive (25 cases) or medication-free for at least three weeks (15 cases) patients with schizophrenia and 41 healthy normal controls matched with sex, age, headedness, and education, were recruited in the study. The Structured Clinical Interview (SCID) was used to render the DSM-Ⅳ diagnoses for schizophrenia or schizophreniform disorders. The intrahemispheric coherences between the parietal and frontal electrode pairs (left: F3/P3; right: F4/P4) and interhemispheric coherences between frontal leads (F3/F4) and parietal leads (P3/P4) in the wide-band alpha frequency and peak frequency of photic driving were calculated at rest and during 1Hz light stimulation, respectively. Tests of exploratory eye tracking movement test (ETM), Wisconsin Card Sorting Test (WCST), and Continuous Performance Test (CPT) were finished in the same working day. Stepwise discriminant function analyses were chosen to re-classify the patients and controls. Results:In the discriminant analyses, 61.7% of the subjects could be correctly classified into groups of patients or normal controls using the EEG coherence alone, 77.27% with ETM measures alone, and 70.6% when WCST and CPT parameters were combined. The correct discriminate rate increased to 81.5% when all the above variables were taken into consideration.Conclusion:In consideration of the heterogeneity of schizophrenia, it is suggested necessarily to combine multiple tests to make discrimination of schizophrenia.

Objective To investigate air quality in the basements of high science-technologic buildings. Methods Continuous tests of air quality parameters of physics, chemistry and microbiology were conducted in 11 rooms of the basement used for working and living. Results At every test point the average room temperature was 25.5 ℃, the average relative humidity was 78.7 per cent, the average wind velocity was 0.057 m/s. Between the relative humidity and temperature(r=-0.850, P