2.Research progress on patient-derived xenograft models of lung cancer
Shoubo CAO ; Shi JIN ; Yan YU
Chinese Journal of Clinical Oncology 2016;43(20):887-891
Patient-derived xenograft models (PDXs) of lung cancer are obtained by directly implanting lung cancer tissue fragments in-to immunocompromised mice. The implanted tumor fragments can be proliferated and passaged in these mice models. The PDXs maintain the tumor microenvironment, histological and pathological characteristics, and tumor biomarkers of the original tumor tis-sues. The PDX also offers an ideal mice model that mimics the human tumor microenvironment. These models have important roles in the pre-clinical evaluation of cancer, the assessment of anti-tumor drug responses, and the analysis of biomarkers. These models also present a new direction for the individualized therapy of lung cancer patients.
3.Study on the function of EMSC in periapical tissues regeneration
Zeyuan ZHOU ; Yan JIN ; Shuxiang YU
Journal of Practical Stomatology 2000;0(05):-
Objective:To study the function of ectomesenchymal stem cells(EMSC) in periapical tissues regeneration. Methods:Tissue engineering technique was applied to compose EMSC-ceramic bovine bone for repairing of a Sprague-Dawley rat model of the periapical tissues defects. Results were observed by HE staining. Results:New pulp-cement like structures were formed and the result of experimental group was better than that of control group. Conclusion: EMSC participates in the process of the periapical tissues regeneration.
4.Preparation of total alkaloids from Sophora alopecuroides freeze-dried powders and investigation of its antitumor effects.
Jia-Fu YAN ; Yan TONG ; Chun PU ; Jin-Yu WANG
China Journal of Chinese Materia Medica 2014;39(7):1234-1237
OBJECTIVETo investigate molding technology of total alkaloids from Sophora alopecuroides freeze-dried powders and observe its inhibition effects on liver transplantation tumor in mice.
METHODWith color, clarity, water-soluble and formability as indexes, single factor tests were adopted to screen type and amount of filler, the concentration of total alkaloids in drug liquid, pH in order to determine optimum prescription of total alkaloids from S. alopecuroides freeze-dried powders, the lowest melting point was determined and freeze drying curve was drafted. Mice hepatoma H22 ascites tumor strain was sterile inoculated in right axillary subcutaneous of mice, and antitumor effect of total alkaloids from S. alopecuroides freeze-dried powders on liver transplantation tumor H22 in mice.
RESULTWhen selected 80 g x L(-1) as mannitol as filler, the concentration of total alkaloids in drug liquid was 25 g x L(-1) and pH 6.5-7.5, freeze-dried effect was optimum with fast reconstitute speed. Average inhibition rate of the big (120 mg x kg(-1)) and medium (60 mg x kg(-1)) dose group of total alkaloids from S. alopecuroides freeze-dried powders on liver transplantation tumor H22 in mice were 56.08% and 35.49%, respectively.
CONCLUSIONPreparation technology was reasonable, reproducible and stable, total alkaloids from S. alopecuroides freeze-dried powders had significant antitumor effect and showed a dose-effect relationship.
Alkaloids ; chemistry ; pharmacology ; Animals ; Antineoplastic Agents, Phytogenic ; chemistry ; pharmacology ; Cell Line, Tumor ; Color ; Freeze Drying ; Humans ; Liver Neoplasms ; drug therapy ; Mice ; Powders ; chemistry ; Sophora ; chemistry ; Tumor Burden ; drug effects
6.Analysis of related factors for medical discipline construction and development
xiao-ying, XI ; jia-yu, YAN ; xiao-jin, WANG ; yan, WANG ; jin, MA
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(09):-
Objective To screen and analyse the dominated related factors for medical discipline construction and development,and provide evidences for the reinforcement of discipline connotation construction.Methods The candidate medical disciplines of State Key Disciplines Evaluation of 2007 were served as study subjects.Disciplines from the results of State Key Discipline Evaluation were divided into "new discipline" group(n=51),"breeding discipline" group(n=33)and "failed discipline" group(n=81).The differences between results of State Key Discipline Evaluation and corresponding data of past years(2001 to 2006)in academic team,scientific research,graduate education and lab construction were analysed by nonparametric Kruskal-Wallis test.Results There were significant differences in academic leader,state key project,prize level,graduates quality and scientific research base among the results of State Key Discipline Evaluation(P≤0.05),and the dominated factors for the unsuccessful result were lack of high-level academic leader,state key discipline,prize,gruaduates and scientific research base.Conclusion The adoption of appropriate measures for the construction of academic team,the reinforcement of scientific research and the establishment of academic achievement evaluation may help to upgrade the academic level.
7.A review of detection methods for human bocaviruses.
Yan LU ; Dan-Di LI ; Yu JIN ; Zhao-Jun DUAN
Chinese Journal of Virology 2014;30(3):298-302
Human bocavirus (HBoV) 1-4 have been detected both in respiratory and stool samples since the first HBoV was discovered in 2005. HBoV-1 is mostly associated with respiratory infection, while HBoV 2-4 are usually associated with intestinal tract infection. A variety of signs and symptoms have been described in patients with HBoV infection, including cough, wheezing, pneumonia, and diarrhea, but the research on pathogenic mechanism of HBoV is limited because HBoV cannot be cultured in vitro due to the lack of appropriate host cells. Three-dimensional epithelial cell culture, reverse genetics, and viral metagenomics are identified as novel tools that may promote the research on pathogenic mechanism of HBoV and the discovery of new viruses. This review summaries currently available diagnostic approaches such as electron microscopy, cell culture, PCR, and immunoassay in order to provide a method reference for indepth research on HBoV.
Animals
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Human bocavirus
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genetics
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growth & development
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isolation & purification
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pathogenicity
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Humans
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Parvoviridae Infections
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diagnosis
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virology
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Viral Proteins
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genetics
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metabolism
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Virology
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methods
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Virulence
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Virus Cultivation
8.Rhinocerebral mucormycosis: report of two cases.
Chun-yan HE ; Yu-lan JIN ; Hong-gang LIU
Chinese Journal of Pathology 2010;39(5):345-346
Adolescent
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Aged
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Aspergillosis
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microbiology
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pathology
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Aspergillus
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isolation & purification
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Brain Diseases
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drug therapy
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microbiology
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pathology
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surgery
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Diabetes Complications
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microbiology
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Diagnosis, Differential
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Female
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Humans
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Male
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Mucorales
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isolation & purification
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Mucormycosis
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drug therapy
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pathology
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surgery
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Nose Diseases
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drug therapy
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microbiology
;
pathology
;
surgery
10.Congenital lower limb lymphedema in a neonate.
Bei-yan ZHOU ; Guang-jin LU ; Yu-kun HAN
Chinese Journal of Pediatrics 2009;47(1):78-78
Humans
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Infant, Newborn
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Lower Extremity
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pathology
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Lymphedema
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congenital
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Male