Objective To study the effects of PM2.5 and PM10 of dust storm on the secretion of nitrogen oxide(NO), interleukin-8 (IL-8) and tumor necrosis factor-?(TNF-?)in the alveolar macrophages(AM)of rat in vitro. Methods PM2.5 and PM10 were collected during dust storm period in the urban area of Beijing. The tested cells were primary cultured alveolar macrophages. Cytotoxicity of the particles was measured by MTT assay. The content of NO was determined using Griess reagent. The levels of cytokines IL-8 and TNF-? were determined using radioimmunity assay. Results After treatment for 24 h, PM2.5 and PM10 caused cytotoxicity to AM and the survival rate of the cells was decreased with the increasing exposure dose of the dust to the rats and the survival rate of the cells in the high dosage group was 80% of the control group. At concentrations of 20～150 ?g/ml, PM2.5 and PM10 increased the secretion of NO, TNF-? and IL-8 in AM in a dose-dependent manner. The cytotoxicity of PM2.5 was stronger than that of the PM10 at the same concentration, while the effects on the increasing secretion of NO, IL-8 and TNF-? induced by PM2.5 was smaller than that of the PM10. Conclusion PM2.5 and PM10 of dust storm could induce the secretion of inflammatory factors NO, IL-8 and TNF-? in rat AM.

Objective To explore the clinicopathological and immunohistochemical features of primary malignant pericardial mesothe- lioma.Methods Seven cases of primary malignant pericardial mesothelioma,in 4 females and 3 males,with the age ranged from 22 to 51 and a mean of 40 years old,were studied with clinicopathological,immunohistochemical and histochemical techniques,The pertinent litera- ture was also comprehensively reviewed.Results 3 biopsy specimens and 4 surgical specimens,including 2 autopsy specimens,were stud- led.The main clinical symptoms were palpitation and short breath,which were aggravated after movement.The longest course was 4 years,and the mean disease course was 12 months.The chest films demonstrated an obviously increase in heart shadow,and the pericardi- um was thickened in some cases.CT clearly showed tumorous shadow in 2 cases.The histopathologic picture showed tubular type in 2 ca- ses,papillary in 2,signet ring cell type in 1 and myofibroblast types in 2.In 2 autopsy specimens it was found that pericardium was dif- fusely thickened.In one case the tumor was metastasized to the right lung,mediastinum,and pulmonary hilar lymph node.In another case there was metastasis to right kidney.Immunohistochemical staining showed that the neoplastic cells of all cases were positive for CK5/6, calretinin,CK.vimentin and EMA,but negative for CEA.In the histochemical staining seven cases,the malignancy was positive for CI to different extent,but negative for HCL Conclusion Immunohistochemistry and histochemistry play an important role in diagnosis and dif- ferential diagnosis for primary malignant mesothelioma and other tumors in pericardium.

Child ; Fibroma ; Humans ; Male ; Nasal Cavity ; pathology ; Nose Neoplasms

Cholestasis, Intrahepatic ; etiology ; Citrullinemia ; complications ; Humans ; Infant ; Male

Objective To assess the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonist on prostate epithelial cells in vitro.Methods The expression of peroxisome proliferator-activated receptor γ(PPARγ) was studied by immunocytochemistry and immunofluorescence study.The RWPE-1 human prostate epithelial cell line was treated with PPARγ agonist rosiglitazone 100 μmol/L for 48 h.Analysis of apoptosis was performed by Caspase 3/7 activity assay.Mitochondria depolarization was measured by using the potential-sensitive color,JC-1.The expression of apoptosis-related proteins-Bax was investigated by immunohistochemistry.Results PPARγ mainly located in nucleus and perinucleus.RWPE-1 cell line treated with PPARγ agonist rosiglitazone showed higher Caspase 3/7 activity (10636±1032 RLU) than in control (5936±620 RLU),P＜0.01 and significantly upregulated Bax level (8250±694 vs.6017±563)than in control group,P＜0.01.In addition,mitochondrial membrane potential was depolarized in rosiglitazone treated cells.Conclusions PPARmay play important roles in the pathophysiology of BPH.The mechanism might be that PPARγ regulates cell apoptosis.It is suggested that the mitochondrial and Bax pathway might be involved in signaling PPARγ induced cell apoptosis.

Objective To establish a HPLC method for quality control of Pei-bao capsule. Methods The content of lysine derivatized from 2,4-dinitoflruorobenzene was determined by HPLC, with chromatographic column being the agilent Zorbax SB-C18 column (250 mm×4.6 mm, 5 μm) , mobile phase being acetonirile-0.04mol/L, potassium dihydrogen phosphate (40 : 60), the flow rate being 1.0 ml/min, the column temperature being 30 ℃, and wavelength being 360 nm. Results It showed good linear coorelation when the concentration of Lysine hydrochloride being within 0.39～ 19.3μg/ml (γ =0.9999) , and the average sample recovery rate was 100.2%. Conclusion This method is simple, accurate, stable and reliable. It can be used for quality control of Pei-bao capsule.

Objective To explore the diagnostic value of prothrombin time(PT),activated partial thromboplastin time(APTT),fibrinogen(FIB),D-Dimer(DD),antithrombin Ⅲ(ATⅢ),platelet(PLT)in activity and severity assessment of ulcerative colitis(UC),and to analyze whether they could evaluate the degree of activity UC in children.Methods The data of UC patients in the Department of Gastroenterology,Children's Hospital of Nanjing Medical University from January 2012 to September 2016 were analyzed,25 cases of remission and 36 cases of active UC patients were selected as the study subjects.Thirty healthy children were selected as healthy control group,combined with the coagulation function indicators for analysis.Results Receiver operating characteristic area under the curve(AUC)of PT,APTT,FIB,DD,ATⅢ and PLT in UC patients was 0.659,0.840,0.744,0.776,0.599 and 0.792,the activity of UC patients was 0.849,0.889,0.836,0.912,0.964 and 0.966,respectively.The results of PT,APTT,FIB,DD and PLT in children with active UC were significantly higher than those in the healthy control group,and ATⅢ was significantly lower than that in the healthy control group.Compared with the healthy controls,the levels in remission UC patients were not significantly different(P>0.05).The 3 subgroups of activity UC had no significant differences among PT and APTT(F=0.652,1.755,all P>0.05),However,there were significant differences among FIB,DD,ATⅢ and PLT(F=66.495,32.817,88.284,22.892,all P<0.05).FIB,DD and PLT were moderately positively correlated with severity of activity UC patients(r=0.857,0.648,0.654,all P<0.05),and ATⅢ had moderately negative correlation(r=-0.789,P<0.05).Progressive regression analysis showed that the severity of UC was associated with FIB,DD and ATⅢ(R2=0.830,F=39.962,P<0.05).Conclusion FIB,DD and ATⅢ can be used as index for the activity and evaluation of UC.

Objective To assess the effectiveness of repeated trans-cranial magnetic stimulation ( rTMS) in relieving post-stroke depression ( PSD). Methods PubMed, Embase, the Cochrane library, Web of Science, CNKI, WANFANG, and VIP were searched for reports of randomized, controlled trials of rTMS treatment of PSD published before June 2015. Crude standardized mean differences ( SMDs) and odds ratios with 95% confidence in-tervals ( CIs) were calculated for depression intensity and effectiveness rate after treatment using random or fixed effects models. Results Twenty-four studies involving 856 rTMS-treated patients and 802 control patients were in-cluded in the meta-analysis. The results showed that compared with the control group, PSD patients showed significant reductions in depression after rTMS treatment ( SMD=-1.36;95% CI-1.6 to-1.12;P≤0.05) . The total effective-ness rate in the treated group was 85% with a reduction in NIHSS score ( SMD=-0.82;95% CI-1.2 to-0.44;P≤0.05) . Subgroup analysis showed that neither the frequency of rTMS stimulation, the site stimulated, nor time after stroke had a significant influence on the effectiveness of rTMS. Additionally, a few studies reported adverse reactions after rTMS. Conclusion rTMS appears to be a safe and effective therapy for PSD. Further well-controlled trials may elucidate the mechanism underlying the placebo effects of the sham rTMS observed among PSD patients.

0.05), after treatment all the indexes in the 2 groups were decreased(P

0.05). Conclusions The expression of angiotensinⅡincreases in BPH tissues in rats. It is suggested that angiotensinⅡmay affect cell proliferation but not significantly affect cell apoptosis of the rat prostate.