1.A case of idiopathic hypereosinophilic syndrome.
Chinese Journal of Pediatrics 2003;41(5):394-394
Anemia
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complications
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Child
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Female
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Fever
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complications
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Humans
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Hypereosinophilic Syndrome
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complications
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diagnosis
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therapy
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Splenomegaly
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complications
2.The effect of health intervention to changeable risk factors in persons with diabetes prophase
Tianjie SUN ; Ying JIN ; Wenxia TAI
Chinese Journal of Health Management 2008;2(1):33-35
Objective To evaluate the value of health intervention to changeable risk factors in persons with diabetes prophase.Methods One hundred and eighteen persons in diabetes prophase were divided into two groups randomly.For 58 persons enrolled in the experimental group,health intervention including lectures of diabetes basic knowledge,introducing the necessity and significance of periodical check up and examination,suitable dietary and nutrition,weight control,physical exercise and movement was performed.For 60 persons enrolled in the control group,only the diagnosis of diabetes prophase was informed.The incidence of normalization of blood glucose and progressing to dominant diabetes was compared between the experimental group and the control group after follow-up for 6 months and 12 months.Results After 6 months of intervention,the difference of the number of persons was not significant for normalization of blood glucose between experimental group and control group(x2=0.04 and 0.79,P=0.84 and 0.12 for persons with IFG and IGT respectively),and was also not significant for progressing to dominant diabetes between the two groups(x2=0.96,P=0.33).After 12 months of intervention,the difference of the number of persons was significant for normalization of blood glucose between experimental group and control group(x2=13.34 and 22.58,P<0.01 and<0.01 for persons with IFG and IGT respectively),but still not significant for progressing to dominant diabetes between the two groups(x2=2.83,P=0.09).Conclusions The intervention to changeable risk factors can prevent or delay the occurrence of diabetes mellitus,and improve the quality of life for person with diabetes prophase.
3.Killing effect of 17-allylamino-17-demethoxygeldanamycin on a breast cancer cell line
Daozhen CHEN ; Lu LIU ; Xinyu JIANG ; Jin SUN ; Ying HUANG
Journal of Medical Postgraduates 2003;0(12):-
Objective:To study the anti-proliferation effects of a heat shock protein 90(Hsp90) inhibitor,17-allylamino-17-demethoxygeldanamycin(17-AAG),on a human breast cancer cell line,SKBr3,and related mechanism.Methods:MTT assay was used to detect the growth inhibition of SKBr3 cells.Cell cycle and apoptosis were analyzed by flow cytometry.Alteration of human epidermal growth factor receptor 2(HER2) in SKBr3 cells being treated with 17-AAG were measured by immunohistochemistry.Results:17-AAG significantly inhibited growth of SKBr3 cells in vitro in a dose-dependent manner with an IC_(50) value at 3.09 ?g/ml.Under concentrations of 0,0.625,1.250,2.500,5.000 and 10.000(?g/ml,)the percentages of cell apoptosis were(1.03?0.08)%,(3.68?0.67)%,(7.06?1.12)%,(11.23?1.36)%,(20.32?1.98)%,and(31.65?2.96)%;the percentages of cells at G_(0)/G_(l) phase were 58.61%,54.34%,49.55%,43.73%,35.52%,and 27.46%;the percentages of cells at S phase were 29.57%,25.21%,19.65%,22.98%,19.71%,and 15.46%;the percentages of cells at G_(2) /M phase were 11.82%,20.45%,30.18%,33.29%,44.77%,and 57.08%,respectively.The level of HER2 expression in SKBr3 cells being treated with 17-AAG,compared to that in control cells,was reduced significantly.Conclusion:17-AAG can inhibit the growth of human breast cancer cell and enhance its apoptosis.It may be a promising anti-tumor drug.
4.THE CORRELATION BETWEEN DUODENAL OXIDATIVE STRESS MEDIATED BY HIGH FAT DIET AND INTESTINAL CALCIUM ABSORPTION IN MICE
Ying XIAO ; Jue CUI ; Yonghui SHI ; Jin SUN ; Guowei LE
Acta Nutrimenta Sinica 1956;0(01):-
Objective To explore the effect of high-fat diet(HFD) on redox states of duodenum and calcium absorption in mice,and to analyze the relation between them.Method Fifty C57BL/6 male mice were randomly assigned to five groups.The control group consumed an ordinary diet(0.6% Ca,w/w),and other four groups were fed with HFD(19.64%lard,0.6% Ca),HFD plus 0.1% lipoid acid(LA),HFD with calcium supplement(1.6%Ca) and HFD with 1.6% Ca and 0.1% LA supplement.Calcium apparent absorption was measured by mineral balance study after feeding for 8 w.Plasma and duodenum levels of ROS,SOD,CAT,MDA,GSH/GSSG,and T-AOC were measured to evaluate the antioxidant status.Results HFD induced oxidative stress of duodenum and decrease of intestinal calcium absorption in mice.There were positive correlations between calcium apparent absorption with GSH/GSSG(r=0.801,P
5.Study on capillary endothelium injury in the lung with ischemia-reperfusion
Jian WU ; Yi JIN ; Ying SUN ; Qinwei ZHENG ; Xinhong TAN
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To confirm the effects of capillary endothelium injury in the lung with ischemia reperfusion. METHODS: The rabbits pulmonary models of ischemia reperfusion (I-R)injury were established. Plasma nitric oxide and endothelin levels, arterial oxygen tension and wet/dry weight ratios were determined in different periods in control and I-R groups, and the pulmonary ultrastructure abnormities were analyzed under electromicroscope. RESULTS: The plasma levels of NO and ET-1 in I-R animals increased significantly ,compared with those in sham treated control groups. The level of ET-1 had significantly negative correlation with PaO 2 and positive correlation with the value of the wet/dry weight ratios. The swelling and karyopyknosis of capillary endothelium in the lung with I-R groups were observed in ischemia periods. The injuries of endotheliums and typeⅠandⅡ alveolar cells in 0 5 h of reperfusion were more severe than those in ischemia time, and the injuries began to repair in 2 h of reperfusion time. CONCLUSION: In the I-R group, pulmonary capillary endothelium have been injuried, which may play a prominent role in I-R injury and dysfunction of the lung.
7.CLUSTERING ANALYSIS ON FEMORAL GENE EXPRESSION PROFILES IN MICE FED HIGH-FAT DIET
Ying XIAO ; Jue CUI ; Yonghui SHI ; Jin SUN ; Guowei LE
Acta Nutrimenta Sinica 1956;0(03):-
Objective To investigate the changes in femoral gene expression profiles in C57BL/6 mice fed high-fat diet (HFD) via clustering analysis of DNA microarray.Method Sixteen male C57BL/6 mice (4w old) were randomly assigned to two groups,8 in each,after 4-d ordinary diet for adaptation.The control group was fed with an ordinary diet,and the HFD group with HFD(19.5% lard).All mice were sacrificed at the end of 12w and the femoral gene expression was detected by oligonucleotide microarray analysis with Affymetrix Gene Chip Mouse U430A.DAVID,an online tool,was used for clustering analysis on femoral gene expression.Results Longtime administration of HFD caused femoral gene expressed differences related to cation ion channel,transcription regulation and signal transduction,bone mineralization,phosphate metabolic process regulation,and collagen synthesis.Conclusion Longtime intake of HFD will change the expression of numerous bone metabolism-related genes in bone of mice,and then inhibit bone formation.
8.Expression of serum AQP4-Ab and the significance in patients with optic neuritis
Feng, ZHAO ; Wei, CHEN ; Qing-Shan, SUN ; Jin-Ying, FU
International Eye Science 2016;16(10):1956-1958
AIM:To investigate the serum antibody of aquaporin 4 ( AQP4 - Ab ) in positive expression rate and the significance in patients with neuritis.
●METHODS: A total of 98 cases ( 128 eyes ) of patients with optic neuritis were studied to detect the patient′s serum AQP4-Ab positive rate of antinuclear antibodies ( ANAs) from Jan. 2012 to Dec. 2015 in ophthalmology center of our hospital. According to the expression of AQP4 - Ab group, the best corrected visual acuity between the two groups, peripapillary nerve fiber layer thickness (pRNFL), the volume of the macula, macular RNFL ( mRNFL ) , macular core layer volume ( mlNL ) measurement were compared.
●RESULTS:Ninety-eight patients (128 eyes) with optic neuritis cases diagnosed through examination revealed AQP4-Ab positive in 22 patients ( 22%) , negative in 76 patients ( 78%) , ANAs positive in 21 patients ( 21%) , negative 77 patients ( 79%) . Optic neuritis patients with serum AQP4 - Ab positive rate and ANAs positive significant correlation ( r = 0. 707, P < 0. 05 ). After examination revealed AQP4-Ab patients and negative-positive patients with best corrected visual acuity difference was not statistically significance (P>0. 05). After inspection found pRNFL, macular volume measured value AQP4 - Ab positive patients were significantly less than the negative patients, the differences were statistically significant (P<0. 05). AQP4-Ab positive patients and negative patients the mRNFL, mlNL measured values were not significantly different (P>0. 05).
●CONCLUSION:AQP4-Ab and ANAs expression in optic neuritis patients is a significant correlation. AQP4-Ab positive patients with optic neuritis pRNFL thinning of macular volume are decreased compared with negative patients.
9.Molecular cloning of tomato LeHsp110/ClpB gene and its effect on the thermotolerance in plant.
Jin-Ying YANG ; Ying SUN ; Ai-Qing SUN ; Shu-Ying YI ; Jian LIU
Chinese Journal of Biotechnology 2006;22(1):52-57
The heat shock protein ClpB is a member of the Clp family and functions as molecular chaperones. ClpB is related to the acquired thermotolerance in organisms. A cDNA of 3144 bp was screened out of a tomato cDNA library. The polypeptide deduced from the longest ORF contains 980 amino acid residues, and was classified into HSP100/ClpB family based on the result of molecular phylogenesis analysis. Thus it was named as LeHSP110/ClpB according to its calculated molecular weight. LeHSP110/ClpB was characteristic of heat-inducibility but no constitutive expression, and was demonstrated to locate in chloroplastic stroma. An antisense cDNA fragment of LeHsp110/ClpB under the control of CaMV 35S promoter was introduced into tomato by Agrobacterium tumefactions-mediated method. At high temperature, the mRNA levels of LeHsp110/ClpB in antisense transgenic plants were lower than those in control plants. The PS II of transgenic plants is more sensitive to high temperature than that of control plants according to data of Fv/Fm. These results clearly showed that HSP110/ClpB plays an important role in thermotolerance of high plants.
Adaptation, Physiological
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genetics
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Chloroplasts
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metabolism
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Cloning, Molecular
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Genes, Plant
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genetics
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HSP110 Heat-Shock Proteins
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genetics
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metabolism
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Hot Temperature
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Lycopersicon esculentum
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genetics
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physiology
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Photosystem II Protein Complex
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metabolism
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Plant Proteins
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genetics
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metabolism
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Plants, Genetically Modified
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genetics
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physiology
10.Recurrent low frequency sensorineural deafness.
Ying LIN ; Jin Ling WANG ; Fei SUN ; Jin Jin SHEN ; Zhao Xia WANG ; Jian Hua QIU ; Ding Jun ZHA
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2018;32(6):474-476
Low frequency sensorineural deafness is a common subtype of idiopathic sudden deafness. Certain patients suffered recurrent attacks without vertigo, much alike Meniere's disease. Few of them developed into definite Meniere's disease during long-term follow-up in many clinical studies. Although the pathophysiology of recurrent low frequency deafness is yet unknown, the desease is considered associated with early state of endolymphatic hydrops or migraine. Otologists shall be aware of its clinical course and careful explanation is necessary at time of initial informed consent.
Endolymphatic Hydrops
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complications
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Hearing Loss, Sensorineural
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complications
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diagnosis
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Hearing Loss, Sudden
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Humans
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Meniere Disease
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complications
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Vertigo