1.The significance of PCD and mRNA transcription of p 53 gene during development of normal palate and cleft palate in C57BL/6N mouse
Xin LIN ; Yan JIN ; Shaozhong DONG
Journal of Practical Stomatology 2000;0(06):-
砄bjectives:To observe the difference of programmed cell death (PCD) and the expression of related gene p 53 in the development of normal palate and in the formation of cleft palate.Methods:The model of the development of cleft palat was estabished with retinoid acid (80 mg?kg 1 for each pregnant mouse). The palate samples were obtained at GD13 14 (at 13rd day 14 hours of prenancy),13 22 ,14 8,14 14 ,14 33 ,15 8,15 22 and 16 8 respectively.PCD was detected with TUNEL staining,while the mRNA transcription of p 53 was observed by in situ hybridization.Rssults:In early development of palate process,the positive index of PCD in the cleft palate samples was significantly higher than that in the normal ( P 0.05) between the two groups.Conclusion:The proliferation of mesenchymal cells of the early developmental palate process may be inhibited due to the abnomal PCD in the formation of cleft palate.The mechanism of PCD in this study may be a p 53 independed pathway.
2.Clinical Study on Qidan Granule for Treatment of 105 Cases of Pulmonary Interstitial Fibrosis
Changjun JIN ; Hongtao XIN ; Dianjie LIN
Journal of Traditional Chinese Medicine 1992;0(08):-
Objective:To investigate clinical therapeutic effect of Qidan Granule on pulmonary interstitial fibrosis(PIF).Methods:The treatment group(105 cases)were treated by Qidan Granule,3 times a day,and the control group(60 cases)were treated by prednisone,0.5mg/kg,for 3~6 months.The patients were followed up once every 1~3 months.Changes of symptoms,signs and high-resolution computed tomogram(HRCT)and pulmonary function were investigated after treatment.Results:After treatment of 6 months,the improvement rate of symptoms and signs in the treatment group was superior to that in the control group(P
3.Expression of HLA-G and IL-10 in patients with acute leukemia.
Jin YANG ; Shao-Lin ZHAO ; Xin-Ling YANG
Chinese Journal of Hematology 2012;33(2):135-137
Acute Disease
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Adolescent
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Adult
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Aged
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Child
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Female
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HLA-G Antigens
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metabolism
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Humans
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Interleukin-10
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metabolism
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Leukemia
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metabolism
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Male
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Middle Aged
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Young Adult
5.Exploration on the Research-oriented Teaching Reform of Microbiology
Yue-Lan YIN ; Xin-An JIAO ; Zhi-Ming PAN ; Lin SUN ; Jin-Lin HUANG ; Xiang CHEN ;
Microbiology 2008;0(12):-
This paper is discussed about course system construction of Microbiology, teaching method, in- struction means and experimental teaching mode. Teaching practice indicated that reform the pattern of Mi- crobiology educational mode can stimulate students’ interest in studying the course, cultivate their inde- pendent ability to solve questions, develop their creative thinking. It is an important way to train high-caliber talents.
6.Study on material base of Carthamus tinctorius with antioxidant effect based on selective knock-out.
Lin-Yan WANG ; Yu-Ping TANG ; Xin LIU ; Ya-Hui GE ; Shu-Jiao LI ; Er-Xin SHANG ; Jin-Ao DUAN
China Journal of Chinese Materia Medica 2014;39(7):1285-1289
OBJECTIVETo establish a method for studying efficacious materials of traditional Chinese medicines from an overall perspective.
METHODCarthamus tinctorius was taken the example. Its major components were depleted by preparing liquid chromatography. Afterwards, the samples with major components depleted were evaluated for their antioxidant effect, so as to compare and analyze the major efficacious materials of C. tinctorius with antioxidant activity and the contributions.
RESULTSeven major components were depleted from C. tinctorius samples, and six of them were identified with MS data and control comparison. After all of the samples including depleted materials are compared and evaluated for their antioxidant effect, the findings showed that hydroxysafflor yellow A, anhydrosafflor yellow B and 6-hydroxykaempferol-3, 6-di-O-glucoside-7-O-glucuronide were the major efficacious materials.
CONCLUSIONThis study explored a novel and effective method for studying efficacious materials of traditional Chinese medicines. Through this method, we could explain the direct and indirect contributions of different components to the efficacy of traditional Chinese medicines, and make the efficacious material expression of traditional Chinese medicines clearer.
Alkalies ; chemistry ; Antioxidants ; chemistry ; Carthamus tinctorius ; chemistry ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; Glucosides ; chemistry ; Mass Spectrometry
7.Study on the Whitening activity and pathway of red eucommia herbs
Jinbin LIN ; Tong REN ; Yijiang LIAN ; Weijie LI ; Xin JIN ; Zhibo XIA ; Wenjun LI
Chinese Journal of Biochemical Pharmaceutics 2015;(12):21-23,28
Objective To determine the impact of PEE with different ethanol concentration extract on the activity of tyrosinase in B16 melanoma and to explore the feasibility of PEE used as skin whitening agent make use of a model organism .Methods 30%, 50%, 70%, 95%, different concentration of ethanol reflux got four kinds of PEE alcohol extract, respectively.And selected the most active inhibition to mushroom tyrosinase ( PEE30、PEE50、PEE70、PEE95).The effect of extracts from the selected on B16 cell model was determined by MTS, NaOH splitting decomposition, L-DOPA oxidation progress.The change in protein expression level after ethanol extract of PEE was determined by Western bolt.Results The capacity of inhibition to mushroom tyrosinase in each concentration of PEE extraction, but PEE70 had the most active inhibition.When challenged with B16 cell model, PEE70 showed the capacity of decrease in tyrosinase activities and melanin synthesis (P<0.01) and had a does dependence ;it also decreased Tyrosinase and TRP-1 expression compared with control B16 cells.Conclusion These data support the idea that PEE can restrain melanogenesis, through its inhibitory effect on the activity of tyrosinase.
8.Effect and mechanism of cell differentiation agent Ⅱ on the differentiation of HL-60 cells
Guorong JIN ; Xiumei LIN ; Yanli XU ; Weiwei LIU ; Xin ZHOU ; Yan TANG ; Huangxuan SHEN
Cancer Research and Clinic 2013;(6):361-364
Objective To investigate the effect and mechanism of cell differentiation agent Ⅱ (CDA-Ⅱ) on the differentiation of human acute mycloid leukemia (APL) HL-60 cells.Methods The cell morphology and differentiation was detected by Wright-Giemsa staining,the expression of cell surface differentiation antigen CD11b of HL-60 was detected by flow cytometry,the differentially expressed genes were screened by gene expression profile chip (NimbleGen).Results The result of Wright-Giemsa staining showed that CDA-Ⅱ induced HL-60 differentiation towards mature stages in a time-dependent manner.After treated with CDA-Ⅱ,the percentage of CD11b-positive HL-60 cells significantly increased in a time-and dose-dependent manner.The result of gene expression profiling indicated differentially expressed genes including 113 up-regulation genes and 140 down-regulation genes.The up-regulation expression genes involved in six pathways including mineral absorption,complement and coagulation cascades,down-regulation expression genes involved in nine pathways including pyrimidine metabolism,RNA polymerase,purine metabolism and so on.Conclusion CDA-Ⅱ can induce HL-60 differentiation and make gene differentially expressed.The data provide the feasibility of CDA-Ⅱ in differentiation induction therapy for APL.
9.Anti-atherosclerosis role of N-oleoylethanolamine in CB2.
Yating GAI ; Qiang SHU ; Caixia CHEN ; Youlin LAI ; Wenjun LI ; Lu PENG ; Limin LIN ; Xin JIN
Acta Pharmaceutica Sinica 2014;49(3):316-21
To observe a PPAR-alpha agonist effect of N-oleoylethanolamine (OEA) on CB2 (cannabinoid receptor 2), an anti-inflammatory receptor in vascular endothelial cell, healthy HUVECs and TNF-alpha induced HUVECs were used to establish a human vascular endothelial cell inflammatory model. Different doses of OEA (10, 50 and 100 micromol x L(-1)) had been given to HUVECs, cultured at 37 degrees C for 7 h and then collected the total protein and total mRNA. CB2 protein expression was detected by Western blotting and CB2 mRNA expression was assayed by real-time PCR. As the results shown, OEA (10 and 50 micromol x L(-1)) could induce the CB2 protein and mRNA expression, but not 100 micromol x L(-1). To detect if anti-inflammation effect of OEA is partly through CB2, CB2 inhibitor AM630 was used to inhibit HUVEC CB2 expression, then the VCAM-1 expression induced by TNF-alpha was detected, or THP-1 adhere to TNF-alpha induced HUVECs was examined. OEA (50 micromol x L(-1)) could inhibit TNF-alpha induced VCAM-1 expression and THP-1 adhere to HUVECs, these effects could be partly inhibited by a CB2 inhibitor AM630. The anti-inflammation effect of OEA is induced by PPAR-alpha and CB2, suggesting that CB2 signaling could be a target for anti-atherosclerosis, OEA have wide effect in anti-inflammation, it may have better therapeutic potential in anti-inflammation in HUVECs, thus achieving anti-atherosclerosis effect.
10.Expressions of Neuron-Specific Enolase and Tumor Necrosis Factor-? Concentrations of Serum and Cerebrospinal Fluid in Children with Epilepsy and Its Significances
wei, LI ; guang-qian, LI ; zhong-dong, LIN ; ying, JIAO ; sheng-xin, JIN
Journal of Applied Clinical Pediatrics 2004;0(08):-
Objective To explore the levels of neuron-specific enolase(NSE) and tumor necrosis factor-?(TNF-?)concentrations of serum and cerebrospinal fluid(CSF) in children with epilepsy,and evaluate its relationships with neuronal damage.Methods Sixty-two epilepsy children were divided into 2 groups:severe group including 28 cases of frenquent seizures ≥3 vices or time of master single test seizures≥15 min,mild group including 34 cases of infrenquent seizures