Adult ; Carcinoma, Papillary ; pathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Middle Aged ; Thyroid Gland ; pathology ; surgery ; Thyroid Neoplasms ; pathology ; surgery ; Thyroidectomy ; methods ; Video-Assisted Surgery ; methods ; Young Adult

Objective To evaluate the effects of early and late tracbeostomy on patients with acute severe cerebral trauma.Methods In the retrospective study,167 patients with severe brain trauma ( GCS ＜ 9 ) requiring prolonged mechanical ventilation ( MV ) were managed by percutaneous dilational tracheostomy (PDT) from May 2001 to December 2010.According to the transoral incubation MV duration,the patients were divided into the early tracheostomy (ET) group ( MV duration≤7 d,81 cases)and late tracheostomy (LT) group (MV duration ＞ 7 d,86 cases).The basic clinical characteristics,pre-and post-PDT MV period,total MV duration,length of post-PDT ICU stay,length of ICU stay,length of hospital stay and mortality were compared between the two groups.Results The two groups showed no statistical differences in aspects of age,sex,acute physiology and chronic health evaluation Ⅱ (A-PACHE Ⅱ ) score,GCS,trauma index and craniotomy rate (P＞0.05).Compared with LT group,ET group significantly shortened the pre-PDT MV period [ (5.16 ± 1.33 ) d∶ ( 1 1.64 ± 4.25 ) d,P ＜ 0.01 ],post-PDT MV period ( median:15.0 d∶ 17.0 d,P ＜ 0.05 ),total MV duration ( median:18.0 d∶26.0 d,P＜0.05),length of post-PDT ICU stay (median:16.0 d∶21.0 d,P＜0.01 ) and length of ICU stay (median:21.0 d∶32.0 d,P ＜0.01 ).But the two groups had no statistical differences concerning the length of hospital stay ( P ＞ 0.05 ),ICU mortality ( 17％ ∶ 14％,P ＞ 0.05 ) and in-hospital mortality (25％∶28.4％,P ＞0.05).Kaplan-Meier curves showed that the frequency of MV and ICU stay rate within 60 days in ET group were significantly lower than those in LT group.Conclusions For patients with severe brain trauma,early tracheostomy shortens the duration of MV and length of ICU stay without affecting their prognosis.Thereby,tracheostomy can be performed at early stage when managing acute severe brain injury.

Objective To explore different risk factors in different age groups patients with large-artery atherosclerotic cerebral infarction,in order to reduce the rates of cerebral infarction and relapse by taking different means of prevention.MethodsOne hundred and ninety-two patients with large-artery atherosclerotic cerebral infarction were divided into three groups by age:youth group(46 cases),middle age group (57 cases) and elderly group (89 cases).The study variables included sex,previous medical history (hypertension and diabetes),long-term smoking,long-term alcohol intake and laboratory parameters (triglyceride,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,homocysteine).ResultsThe rates of high triglyceride and long-term smokingin youth group were significantly higher than those in elderly group [ 60.9％ (28/46) vs.22.5％ (20/89),65.2％ (30/46) vs.29.2％ (26/89),P ＜ 0.05 ].The rates of male,long-term smoking and long-term alcohol intake in middle age group were significantly higher than those in elderly group [ 80.7％ (46/57) vs.59.6％ (53/89),57.9％ (33/57) vs.29.2％ (26/89),47.4％ (27/57) vs.15.7％ (14/89),P ＜ 0.05 ].The rates of hypertension,diabetes and long-term alcohol intake in middle age group were significantly higher than those in youth group [ 84.2％ (48/57) vs.60.9％ (28/46),54.4％ (31/57 ) vs.26.1％ ( 12/46 ),47.4％ (27/57) vs.23.9％ ( 11/46 ),P ＜ 0.05 ].The rate of high triglyceride in youth group was significantly higher than that in middle age group[60.9％(28/46) vs.29.8％(17/57),P ＜ 0.05 ].The rate of hypertension in elderly group was significantly higher than that in youth group [ 83.1％(74/89) vs.60.9％ (28/46),P ＜0.05].There was no significant difference in low-density lipoprotein cholesterol,homocysteine and high-density lipoprotein cholesterol in the three groups(P ＞0.05).ConclusionsKey point is different for the prevention of large-artery atherosclerosis cerebral infarction of different age paragraph.The youth patient emphasizes the change of lifestyle.Middle age patient has more emphasis on blood pressure,blood glucose control of the risk factors based on the lifestyle change.The key of elderly patient is the control of blood pressure.

The number of application and granted funds for NSFC were analyzed statistically in Urology institute of Peking University from 2001 to 2007.The result showed the development of urological research projects and scientific research potential of institute of Urology in recent years.Meanwhile,some suggestions for existing problems of the scientific research were put forward in this paper.

AIM: To investigate the new processing method of Radix Polygonum Multiflorum,determine the content of 2,3,5,4′-stilbene glucoside by HPLC. METHODS: Referring to content of 2,3,5,4′-stilbene glucoside as marker,we have screened out processing time for Radix Polygonum Multiflorum by orthogonal design, by HPLC. RESULTS: 48 h of steaming time can guarantee processing quality of Radix Polygonum Multiflorum. CONCLUSION: The method can be used for processing standard of Radix Polygonum Multiflorum

In the cultural construction of independent institute,there exist the contradiction between tradition and innovation,cultural conflicts between private enterprises and the university.Therefore,adhering to the people-centered concept,coordinating tradition and innovation,merging the advantages of university culture and private enterprises culture into a whole,cultivating the unique spiritual culture,harmonious system culture and unified material culture of independent institute,forming the distinctive “Institute Culture” with its own cultural tradition will provide reference for the theory and practice to enrich and improve the connotative development of independent institute.

Cathepsin B from Helicoverpa armigera (HCB) belongs to the group of cysteine proteinases. HCB is proved being involved in the degradation of yolk proteins during embryonic development,which is an acidic preferring enzyme and is resistant to SDS. The expression of the proenzyme may offer a model for investigating the activation of the enzyme. The HCB gene was constructed into pPIC9K and expressed in Pichia pastoris KM71 strain . After induction by methanol, HCB was expressed and secreted into the medium. The molecular weight of the recombinant procathepsin B was determined as about 38 kDa. The expressed product was confirmed to be HCB by immunoblotting assay using specific rabbit anti-HCB polyclonal antibody. The activity of the product was assayed by in situ hydrolysis (gelatin-SDS-PAGE). These results showed that HCB with proteolytic activity was expressed in P. pastoris KM71. This proenzyme can be used for further research on the activation of the proenzyme or industrial production.

Objective To analysis the clinical features of dermatomyositis(DM)complicated with acute interstitial pneumonia(AIP),and to explore the cause of the disease to improve the treatment.Methods Seven cases of dermatomyositis complicated with AIP in our hospital from 1997 to 2005 were studied retro- spectively and the literatures were reviewed.Resets Seven dermatomyositis patients had typical cutaneous vasculitis with slight symptom of myositis.The electromyogram showed myogenic damage.The Jo-I antibodies were all negative.Simultaneously or shortly after the diagnosis of dermatomyositis,all patients presented with high fever,dry cough and progressed dyspnea.The chest X-ray or high-resolution computed tomography dis- played diffuse pulmonary interstitial changes which progressed rapidly.All patients died despites anti-infec- tious treatment and high dose steroid therapy.Conclusion AIP is the pulmonary injury of amyopathic or hy- pomyopathic dermatomyositis.In order to improve the prognosis,early diagnosis should be emphasized and in- terdisciplinary cooperation should be strengthened.

Objective: To explore the relationship between the ratio of neutrophil/lymphocyte (NLR) and in-hospital major adverse cardiac events (MACE) in patients with acute ST-elevation myocardial infarction (STEMI) at early admission.
Methods: A total of 420 acute STEMI patients admitted and received primary PCI in our hospital from 2010-01 to 2014-12 were retrospectively studied. The patients were divided into 2 groups:In-hospital MACE group, n=47 and Normal discharged group, n=373. Uni-and multivariate analyses were conducted to assess whether high NLR is the independent predictor for in-hospital MACE occurrence.
Results: Univariate regression analysis indicated that the occurrence rate of in-hospital MACE in high NLR patients were higher than those in low NLR patients (OR=3.19, 95%CI 1.55-2.65, P=0.012). Multivariate regression analysis showed that high NLR was the independent risk factor for in-hospital MACE occurrence in STEMI patients (OR=3.05, 95%CI 1.59-10.54, P=0.015).
Conclusion: High NLR is the independent risk factor for in-hospital MACE occurrence in STEMI patients at the early admission.

Objective To summarize the experiences in using Bio-Gide and Bio-Oss for guided bone regener- ation in dental implantation.Methods In 28 cases of bone deficiency,Bio-Gide membranes were applied to cover alveolar defects filled with the Bio-Oss bone powder.In postoperative periodic follow-.up,the bone regeneration effect was observed by successive clinical and X-ray examination.Results 38 implants were inserted in the 28 patients and Bio-Gide membranes were used in the sites of the 38 implants.Alveolar bone defects were filled with new bone in 27 patients,1 implant loosed because of inflammation.37 implants had ideal osseointegration at stageⅡsurgery and were prosthetic rcconstructed successfully.No implant loosed during the observed period of 15 months to 4 years. Conclusion Bio-Gide and Bio-Oss have ideal effect of guided bone regeneration in dental implantation.