ObjectiveTo study the impact of high chromium yeast on type 2 diabetic mice.MethodsA total of 60 Balb/C mice were used in this study.Fifty mice were injected with streptozotocin to induce type 2 diabetes,while the ten mice without injection were in the normal group.The type 2 diabetic mice were divided into 5groups according to their fasting blood sugar levels,including 3 groups receiving high chromium yeast at low [250μg/ (kg· d)],medium [500 μg/ (kg,d)],and high [1000 μg/ (kg· d)] doses,1 group receiving metformin ( positive control),and 1 group receiving normal yeast ( negative control).The yeast or metformin were given through by gastric lavage daily for 15 weeks.Body mass and blood biochemical indexes (fasting blood glucose,glucose tolerance,etc) were determined,and the pathological examination of pancreas was conducted.Results Compared with negative control group,medium dose high chromium yeast group had significantly lower triglyceride ( P =0.043 ),low and medium dose groups had lower total cholesterol ( P =0.006,P =0.003 ),low and high dose groups had higher insulin levels (P =0.011,P =0.002),and high dose group had significantly reduced glycosylated hemoglobin (P =0.027).Pathological examination of pancreatic islets revealed that medium and high dose groups did not develop infiltration of inflammatory cells.ConclusionsHigh chromium yeast may improve type 2 diabetes mellitus.Further studies are needed to confirm the conclusion of this study with an organic chromium control group.

Objective To select the optimum bases for Sihuang Gao and study the method for quality control.Method Ultra-fine powders of Radix Scutellariae,Cortex Phellodendri,Radix et Rhizoma Rhei and Rhizoma Coptidis were used to prepare Sihuang Gao with different formulations,the bacteriostatic action of which were compared and stability were inspected.The best prescription was identified by TLC,and the skin irritation was observed.Result Sihuang Gao made of water-soluble base had a strong antibacterial activity and stable quality,and it was proved no irritation,the contents of active component could be detected by TLC.Conclusion The quality was controllable,and the improvement prescription can be used in the clinic.

Congenital Abnormalities ; Encephalocele ; Humans ; Nose Diseases ; congenital

Child ; Child, Preschool ; Endoscopy, Digestive System ; Female ; Follow-Up Studies ; Humans ; Infant ; Male ; Peptic Ulcer

Objective To optimize the preparation process of Xiaochuan Tiegao. Methods The effective compound imporatorin of Dahurian Angelica Root was determined by HPLC. The best condition of preparation process for the ethanol-extraction method was investigated by L9(34) orthogonal design. Results The optimum technical condition:adding 2 times ethanol, cooking 1 hour and 2 times with 90% ethanol. Conclusion The method is reliable, accurate and easy to operate.

Objective To develop an HPLC method for the assay of sinapine thiocyanate in Semen Sinapis Albae of different processed products. Methods It was assayed by RP-HPLC. The column was Hypersil BDS Phenyl (5 ?m, 250 mm?4.6 mm), the mobile phase was acetonitrile-0.08 mol/L KH2PO4 (15∶85). The UV detection wavelength was 240 nm. The flow rate was 1.0 mL/min. Results Good linearity of sinapine thiocyanate was showed within the range of 0.8～4.0 ?g (r=0.999 7). The average recovery was 100.98% (RSD=3.51%). Conclusion The method is simple, accurate and good reproducible. It can be used for quality control of Semen Sinapis Albae of different processed products.

Moyamoya disease is a chronic progressive cerebrovascular disease which is characterized by progressive stenosis or occlusion at bilateral terminal portions of the internal carotid artery and its large branch vessels,and with an abnormal formation of new vascular network in skull base.As a noninvasive method,magnetic resonance imaging (MRI) plays an important role in the diagnosis and evaluation of moyamoya disease.This article reviews the application of MRI in the identification,diagnosis and treatment evaluation of the stroke subtypes in patients with moyamoya disease.

Objective To demonstrate the mechanisms underlying the efficacy of telmisartan in diabetic nePhroPathy, and discuss the role of PPARγin this Process. Methods The diabetic nePhroPathy rat models were established and randomly assigned to control grouP, telmisartan grouP ( 5 mg · kg-1 · d-1 ) and combination of telmisartan and PPARγ inhibitor grouP (telmisartan:5 mg·kg-1·d-1;GW9662:0. 5 mg·kg-1·d-1). After 12 weeks of treatment,the biochemical indexes of blood and urine,kidney weight,renal Pathology in each grouP of diabetic nePhroPathy rats were measured and comPared. The leVels of IL_1,IL_6 and TNF_α in blood of each grouP were detected by ELISA and comPared. The leVels of HGF and actiVated NF_κB (P65) in renal tissue of each grouP were detected by Western blotting and comPared. Results In diabetic nePhroPathy rats, telmisartan lowered the leVels of serum glucose, serum creatinine, urinary Protein and kidney weight, decreased the glomerular Volume,mesangial Proliferation and inflammatory cell infiltration,reduced blood leVels of IL_1,IL_6,and TNF_α,and decreased leVel of actiVated NF_κB (P65) in renal tissue. The leVel of HGF in renal tissue was eleVated by telmisartan. NeVertheless,these changes were Partly reVersed by PPARγ inhibitor GW9662. Conclusion PPARγ Presents an imPortant role in treatment of diabetic nePhroPathy by telmisartan.

Objective:To investigate the effect of the peritoneal fluid (PF) in women with endometriosis (EM) on the natural killer (NK) cell activity, and to study its relationship with the level of prostaglandins(PGs) and interleukin 6(IL 6). Methods:HeLa cells were taken as target cell, the LDH releasing assay was used to measure NK cell activity after preincubation with PF of EM. The concentration of IL 6, PGE 2 and PGF 2? was measured by radioimmunoassay. Results:After incubated with PF of women with and without EM for 2 h, the inhibition percentages was(65.2?21.8)% and (37.1?18.9)% respectively ( P

Objective To investigate the existence of the erythropoiesis inhibitors(?).Methods Twelve patients suffered from uremia with anemia were studied[5 males,and 7 females,(50?12)years].Methylcellulose culture technique was used to culture mice bone marrow cells.The sera from the uremia patients were added to CFU- E and BFU-E culture medium with final concentrations of 1,25%,2.5% and 5%,Mice bone marrow cells were ob- tained from the female Balb/c mice.In vitro CFU-E and BFU-E culture in the presence of sera from uremia patients was compared with that in the presence of normal human subjects with the use of normal mice bone marrows.Re- suits The effects of the sera from uremia patients on CFU-E and BFU-E colon growth were in a dose-dependent manner.The effect was correlated with the concentrations of the sera(P