Objective Tofollowupandanalyzetheoccurrenceofcerebrovascularischemiceventsin patientswithinternalcarotidarteryocclusion(ICAO).Methods Atotalof144consecutivepatients with ICAO admitted to the Department of Neurology,the Air Force General Hospital from January 2007 to January 2012 were enrolled retrospectively. All patients were confirmed as unilateral ICAO with DSA and they were divided into either a symptomatic group (n=74)or an asymptomatic group (n=70)according to whether they had the symptoms of ischemic cerebrovascular disease or not. Both patients of the 2 groups were followed up for at least 2 years. Their transient ischemic attack (TIA)and endpoint events were observed,including new symptomatic cerebral infarction,death from any cause,as well as patency of collateral circulation,andthefollow-upresultsbetweenthetwogroupswerecompared.Results Twenty-one patients (28. 4%)of the symptomatic group had new attack of cerebral infarction,10 cases (13. 5%)had TIA,and 4 cases (5. 4%)died;9 patients (12.9%)of the asymptomatic group had new attack of cerebral infarction,4 cases (5 . 7%)had TIA,and no patients died. Risk of recurrent stroke in patients of the symptomatic group was higher than that of the asymptomatic group (P=0. 025). Kaplan-Meier curves showed that the incidence of endpoint event of the symptomatic group was higher than that of the asymptomatic group (χ2 =8. 428,P =0. 004). There was significant difference in the patent ratio of the primary collateral circulation between the symptomatic group and the asymptomatic group (64. 9%[48/74] vs. 91. 4%[64/70];P=0. 000);the patent ratio of the primary collateral circulation in patients with new cerebral infarction was significantly lower than that in patients without new cerebral infarction (60. 0%[18/30]vs.82.5%[94/114];P=0.013).Conclusion ThepatientswithsymptomaticICAOaremore likely to have cerebral infarction,and they are likely to die. After ICAO,the primary collateral circulation patent has a significant effect on the prognosis.

Objective To explore the applicative effect of the clinical cluster management during perioperative period on esophageal cancer in elderly patients aged 75 years and over.Methods A total of 36 cases with esophageal cancer aged 75 years and over admitted into Thoracic Surgery Department in our hospital from January 2010 to February 2015 were divided into experimental and control group.Patients in experimental group received clinical cluster management including preoperative therapy for improvement of heart and lung function, selective protection during operation, strengthening support after operation and early nasal tube nutrition.Patients in control group received routine treatment for esophageal carcinoma during perioperative period.The postoperative complications, intensive care unit (ICU) stay, the incidence of anastomotic leakage,postoperative feeding time, postoperative hospitalization time and other indicators were observed.Results Among 20 patients in the experimental group, postoperative cardiopulmonary complications were found in 6 cases, postoperative cardiovascular complications in 3 cases and anastomotic leakage in 2 cases.Among 16 patients in the control group, postoperative cardiopulmonary complications were found in 12 cases, postoperative cardiovascular complications in 10 cases and anastomotic leakage in 5 cases.The incidences of postoperative cardiopulmonary and cardiovascular complications were lower in experimental group than in control group (x2 =7.200 and 8.693, P =0.007 and 0.003).The incidence of anastomotic leakage showed no significant difference between the two groups (x2 =2.562,P=0.109).The mean duration of ICU stay and postoperative hospitalization time were shorter in experimental group than in control group[(5.6±2.7) d vs.(9.8±4.9) d, (15.4±4.5) d vs.(20.6±5.5) d, t=3.208 and 3.125, P=0.003 and 0.004].There was no significant difference in postoperative feeding time between the two groups [(8.4±1.5)d vs.(9.1±1.1)d, t=1.569, P=0.126].Conclusions A tightly clustered clinical managements during perioperative period can minimize the incidences of postoperative complications and mortality rate in elderly patients with esophageal carcinoma.

[Objective]To investigate the effects of doxycycline on rabbit immobilized osteoarthritis and their mechanism.[Method]Twenty-four rabbits were randomly divided into normal,model,control and treatment groups,with 6 rabbits in each group.All of the animals were immobilized the lower left limbs with plaster bandage for 4 weeks except normal group.Doxycycline was orally given to treatment group.Cartilage degeneration of the medial condyle of femur was observed at 4 weeks and the levels of collagen-Ⅱ,uPA and MMP-13 were tested.[Result]Cartilage degeneration in treatment group was significantly slighter than those in model and control groups.Data of collagen-Ⅱ,uPA and MMP-13 between model and control groups had no significant difference,but a noticeable statistical difference among other groups.The uPA activity was positively correlated with MMP-13.[Conclusion]Doxycycline inhibits the degeneration process of cartilage and improves the structure.The effects may be related to the inhibition of uPA and MMP-13.

Objective: To study the mechanism of differentiation of rat ectomesenchymal cells to odontoblasts. Methods: Ectomesenchymal cells were cultured in three-dimension culture model using collagen gel as frame, and the change of phenotype of ectomesenchymal cells were observed and detected by phase-contrast microscopy and immunohistochemistry after the cells had been treated by 10 ng/ml of bFGF or/and 100 ng/ml IGF-1. Results: 4 days after treatment by bFGF and IGF-1, the cells appeared to be odontoblast-like cells aligned parallelly and polarized with long cytoplasmic processes attached to one end of the cell body.The cells were positive for DSP expression. However, the cells were DSP negative and aligned disorderly in other groups. Conclusion: Ectomesenchymal cells can be induced to differentiate to odontoblast-like cells in three-dimension culture model with the treatment by bFGF and IGF-1.

AIM: To evaluate the effects of all-trans retinoic acid (atRA) on the proliferation in cultured mouse cerebral microvascular endothelial cells (bEnd.3) . METHODS: Cultured cells were divided into five groups randomly, one as control group, the other four groups were 10-9, 10-8, 10-7 and 10-6 mol/L group. Effects of atRA on proliferation in bEnd.3 cells were detected by flow cytometry and immunocytochemitry of PCNA and MTT at 24 h, 48 h and 72 h. The effects of atRA (10-6 mol/L group) on the expressions of angiogenic genes in bEnd.3 cells were studied using microarray. RESULTS: The results of MTT and flow cytometry showed that all- trans retinoic acid at concentration of 10-6 mol/L significantly inhibited the proliferation of bEnd.3 cells. Immunocytochemical staining showed the expression of PCNA was markedly decreased in bEnd.3 cells at 24 h after treatment with atRA. Microarray results demonstrated that there were 11 down - regulated angiogenic genes and 2 up - regulated angiogenic genes in 10-6mol/L atRA group. CONCLUSION: All - trans retinoic acid at concentration of 10-6mol/L may significantly inhibit the proliferation of bEnd.3 cells treated for 24 h ire vitro via down-regulation of angiogenic genes and PCNA expression.

Inflammation has a critical role in the pathogenesis and progression of cancer.lymphocyte and monocyte play an important role in inflammatory reaction,reflecting body immune status.In recent years,more and more clinical studies have shown that pretreatment of lymphocyte-to-monocyte ratio(LMR)in the patients with malignant tumor may have a certain value in evaluating the prognosis of cancer patients such as nasopharyngeal cancer,esophageal cancer,breast cancer,ovarian cancer,lung cancer,gastric cancer,colorectal cancer,etc.This article reviews the latest research progress on the value of LMR in the evaluation of the prognosis of the patients with malignant tumor.

Objective To evaluate the immediate results and the 6 month angiographic restenosis rate after repeat percutaneous intervention for in stent restenosis (ISR) Methods The acute and 6 month follow up outcomes of 156 patients with ISR who were treated by repeat PTCA or stenting were analyzed in this retrospective study Quantitative coronary angiography analyses (QCA) were performed before and after repeat intervention for in stent restenosis and on a average 6 month coronary angiogram to assess the recurrent restenosis rate Results All the patients were treated successfully Balloon angioplasty was performed in 134 patients (85 9%) and repeat stenting in 22 patients (14 1%) The six month follow up coronary angiogram showed the recurrent restenosis rate was 24 3% The recurrent restenosis rate was higher in 40 diffuse ISR lesions than that in 96 focal ISR lesions: 45% vs 18%, P75% was also the risk factor of recurrent restenosis Conclusion For the most patients with ISR, repeat PTCA or intracoronary stenting seems to be an effective and safe method The overall restenosis rate after repeat intervention for ISR is the same as that of initial intracoronary stenting

Objective To study the effects of hyperglycemia plasma glucose and insulin intervention on the cerebral injury after ischemia-reperfusion in rats.Methods 160 male SD rats were randomly divided into four groups:sham operation group,normal plasma glucose group,hyperglycemia group and insulin intervention group(40 rats in each group).The rats of hyperglycemia and insulin intervention groups were given STZ to induce hyperglycemia model.All the rats except for those in the sham operation group were subjected to right middle cerebral artery occlusion and then reperfusion.The reperfusion was started at 1 h,6 h,12 h and 24 h after 2 h ischemia.The neurologic functional scores,infarct volume and numbers of neuron apoptosis of the rats were analyzed with the neurological severity score(NSS),TTC staining assay and TUNEL method,respectively after the 2ats wake up.Results Compared with normal plasma glucose group,worse neurological function score,larger infarct size and more number of apoptotic neurons were found in hyperglycemia group at the same reperfusion time(all P

0.05).But for the patients with wide-base vocal cord polyps,the effective rate of self-retaining laryngoscope group was higher than that of electronic laryngoscope(P

At present, lung cancer has entered a new era of immunotherapy. However, a single immunotherapy is unable to satisfy the requirements of the immune microenvironment. More and more different combined immunotherapy schemes have become effective methods to overcome immune resistance. As a promising target in the novel immunotherapy, cancer-testis antigen (CTA) is a highly specific tumor antigen to produce a powerful anti-tumor immune response that is almost only expressed in malignant tumors. This article reviews the research progress of CTA's expression, function and immunotherapy application in lung cancer.