Objective To explore the health effect of traffic exhausts on children's behavioral problems by using the Achenbach's Child Behavior Checklist.Methods Three primary schools were chosen based on the counts of passed by automobiles and the monitoring data of ambient air pollutants.Air pollutants including NO_x,SO_2,PM 2.5 and CO were analzed around the schools.There were 1 821 students in total from Grade 2 to Grade 5 were chosen as the target population and were investigated with the self-designed questionnaire as well as Achenbach's Child List.Finally,1 363 cases were analyzed,of whom have been local resident for more than 2 years and were in condition during investigation.Results A total of 141 children,with the rate of 10.34%,were reported to be with behavioral problem.The reported rates of behavioral problem were 9.04% in School A1, 9.77% in School A2,and 11.99% in School A3 respectively,however,there was no significant difference was seen(x~2=2.556,P= 0.279).The reported rate of behavioral problem of boys' was higher than that of girls' in all schools.Analysis of 11 behavioral problem factors showed that,highest rates of factor depression,social withdrawn,hyperactivity,sexual problem,cruelty,forcing were found in School A3.Single-factor analysis showed that fever experience,eye sight condition,drinking experience of children, experience of being beaten and scold,second hand smoking,drinking habit of father,occupation of parents,education level of parents,born of full-term,medical history in neonatal period,history of unhealthy pregnancy of mother,history of touching adverse factors and drinking experience of mother during pregnancy had significant influence on the reported rate of behavioral problem. Conclusion Traditional factors are still the main influencing factors of children's behavioral problems,long term-low level exposure to traffic exhausts has weak effect on children's behavioral problems.

Objective To investigate the effects of metformin on plasma ghrelin and peptide YY (PYY)levels in newly-diagnosed type 2 diabetic patients,and to study the impact of metformin on body weight.Methods A prospective nested case-control study was designed as a research protocol.Sixty four newly-diagnosed type 2 diabetic patients were treated with metformin for 12 weeks.The patients were divided into two groups:weight loss group and non-weight loss group according to the changes in body weight after metformin treatment.Fasting plasma ghrelin and PYY levels and other metabolic parameters were measured before and after metformin treatment.ResultsFasting plasma ghrelin level was significantly decreased in the patients after metformin treatment [ ( 10.71 ±2.68 vs 11.81 ±3.19 )ng/ml,P＜0.05 ].Fasting plasma PYY level was significantly increased in patients after metformin treatment [ ( 136.86+39.14 vs 128.42+37.31 ) pg/ml,P＜0.05 ].After metformin treatment,43.7％ of the patients lost body weight significantly.Fasting plasma ghrelin level was decreased by 16.6％ after treatment in the weight loss group,as compared with 6.2％ in non weight loss group( P＜0.05 ).Fasting plasma PYY level was increased by 10.8％ after treatment in the weight loss group,as compared with 3.5％ in the non-weight loss group (P ＜ 0.05 ).Conclusions The fasting plasma ghrelin level in the weight loss group was lowered more significantly compared with that in the non-weight loss group after metformin treatment.The fasting plasma PYY level in the weight loss group was elevated more significantly as compared with that in the non-weight loss group after metformin treatment.The mechanism remains to be further studied.

Objective To know the mastery degree of percutaneous coronary intervention (PCI) preoperative nursing knowledge in nurses who are working in emergency department and cardiac ward. Methods 158 cases of nurses who came from three different class Ⅲ grade hospitals with qualification for emergency PCI surgery of the Guizhou province were surveyed with self-made questionnaire. The survey was conducted in nurses who were working in different departments, and the educational background, age, title, category, the cognitive situation of the PCI preoperative nursing knowledge and necessity of preoperative preparations were investigated in nurses. Results The whole pass rate was 48.10%; high qualification, high education, higher title of nurses for the nursing knowledge of the emergency PCI were better; the emergency PCI preoperative preparations for the necessity of cognitive about nurses were on the low side. Conclusion The cognition of the nursing knowledge and the necessity of emergency PCI in preoperative preparations as whole are on the low level, so specialized training should be strengthened to enhance the ability of specialized nursing.

Objective To discuss the significance of acoustic rhinometry(AR) and rhinomanometry(RM) in the evaluation of surgical treatment of nasal septal deviation.Methods Fifty-one patients of nasal septal deviation were recruited,and seventy-two nasal cavities were tested(twenty-one patients with narrowing of double sides).Visual analogue scale(VAS) was used to estimate the degree of nasal obstruction.AR and RM were used to obtain the data of nasal airway resistance(NAR),nasal cavity 0-5cm volume(NCV0-5) and nasal minimal cross-sectional area(NMCA).The data were used to assess the airflow function of nasal cavity.Each patient was tested at the time both before surgery and 12 weeks after surgery.The pre-and post operative data were used to calculate paired t-test by SPSS 16.0,and to disclose the correlation among ?NCV,?NMCA,?NAR and ?VAS.Results The preoperative data showed that NCV0-5 was 4.07?0.91cm3,NMCA 0.33?0.08cm2,NAR 1.14?0.34kPa?s?L-1,and VAS 7.36?1.23;and the postoperative data showed that NCV0-5 was 7.29?0.68cm3,NMCA 0.56?0.08cm2,NAR 0.35?0.12kPa?s?L-1,and VAS 1.14?0.91.Significant differences existed in NCV0-5,NMCA,NAR and VAS(P

Objective:This article was a retrospective analysis on the preoperative CT scan of fungal sinusitis patients.The non-distinctive clinic feature was suggested to improve the accuracy of preoperative diagnosis.Method:A retrospective analysis was made for 176 patients with fungal sinusitis,which were confirmed by postoperative pathologic disanosis from January 2000 to January 2008.Every patient was performed both CT examination and nasalendoscopy preoperatively. All patients underwent endoscopic sinus surgery and the lesions in sinus were cleared and confirmed fungal infection by pathology.Thirty-five cases fungal infection located in sphenoid sinus,84 cases in maxillary sinus,49 cases in both maxillary and ethmoid sinus,3 cases in all sinus,5 cases in both ethmoid and sphenoid sinus.There were 179 sinuses in 176 patients were infected. Result:This group totally had 175 cases(179 sides). One hundred and seventy-three cases had unibilateral lesion,3 cases had bilateral lesion. The CT scan showed non-distinctive features including unifom dense,uneven dense in the sinus and bone resorption of the inner side bone wall. Five cases in sphenoid sinus lesion were found calcification in CT scan but 8 cases didn't show such features.Among these cases. 5 cases showed uniforra.dense and 3 cases showed uneven dense. In 84 cases maxillary sinus lesion,there were 12 cases without distinctive change,among these cases,8 cases showed uniform derise,4 cases showed uneven dense and 5 cases had boRe resorption. In 49 cases both ethmoid and maxillary sinus lesion,13 cases didn't show distinctive change,among these cases,9 cases showed uniform dense,4 cases showed uneven dense and 6 cases had bone resorption. Five cases had calcification in the both sphenoid and ethmoid sinus. Totally 81.56% of these patients had calcification feature in the CT scan.Conclusion:The calcification in the lesion of sinus is usually featured as characteristic manifestation of fungal sinusitis. But in clinic,the calcification does not exit in some patient's CT scan. When being absent of the featured sign in CT scan,the fungal sinusitis have to be diagnozed by other detecting means,even postoperative pathology.

OBJECTIVETo analyze the parameters of urodynamic tests for patients with type-III B prostatitis and evaluate the significance of the results of urodynamic tests in the choice of therapies for this disease.

METHODSUrodynamic tests were performed for 87 type-III B prostatitis patients aged 22-45 (30.7 ± 8.5) years, who had moderate or severe lower urinary tract symptoms (LUTS) and failed to respond to routine therapy. Different treatments were administered according to the results of urodynamic tests followed by observation of the therapeutic effects.

RESULTSUrodynamic abnormalities were found in 70 of the 87 patients, bladder outlet obstruction in 28 (32.2%), detrusor overactivity in 25 (28.7%), bladder hyperesthesia in 18 (20.7%), low compliance in 10 (11.5%), detrusor-external urethral sphincter dyssynergia in 1 (1.1%), and impaired detrusor contractile function in 1 (1.1%). Treatments achieved obvious effectiveness in 26 cases (29.9%), effectiveness in 51 (58.6%), and no effectiveness in 10 (11.5%).

CONCLUSIONUrodynamic tests contribute significantly to the choice of therapies for type-III B prostatitis patients with moderate or severe LUTS.

Adult ; Humans ; Lower Urinary Tract Symptoms ; physiopathology ; therapy ; Male ; Middle Aged ; Prostatitis ; physiopathology ; therapy ; Urethra ; physiopathology ; Urinary Bladder Neck Obstruction ; physiopathology ; Urinary Bladder, Overactive ; physiopathology ; Urodynamics

The specific fragment of Pneumococcal surface protein A(PspA)and Pneumococcal Surface Adhesin A(PsaA)gene was amplified by PCR from Streptococcus pneumonia 5 and Streptococcus pneumonia 19.The amplified fragnent of PspA and PsaA gene was ligated into pET-27b(+)vector and transformed into BL 21 E.coli for expression and obtain the expressive production of PspA and PsaA.Induced by IPTG,the expression level was as high as 75 % of the total disolube protein.The result showed that the recombinant plasmid could express a specific 75 kDa and 37 kDa fusion protein in E.coli BL 21,which showed the good immunogenicity and a broadly cross reactivity with the other serotypes.

This study mainly explored the pulse parameters in children with RRTI with different TCM syndromes,aiming at providing therapeutic indexes and objective basis for its diagnosis and treatment.Three hundred and forty-eight cases of RRTI were divided into five groups,including the group of qi deficiency in the lung (or Fei Qi Xu,FQX),the group of invasion of the lung by wind-heat (or Feng Re Fan Fei,FRFF),the group of invasion of lung by wind-cold (or Feng Han Fan Fei,FHFF),the group of obstruction of phlegm-damp in the lung (or Tan Shi Zu Fei,TSZF) and the group of obstruction of phlegm-heat in the lung (or Tan Re Yong Fei,TRYF).65 children of good health were involved in the control group.Z-BOX pulsemeter apparatus was applied to the paraticipants for analyzing their pulse parameters.As a result,it was found that values of h1,h3,h4,h5,t and h4/h1 of RRTI children decreased,compared with the children of good health (P ＜ 0.01);while h1,h3,h4 and h5 of children in FQX group declined (P ＜ 0.01);and the values of h4,h5,t,w,h3/h1,h4/h1 and h5/h1 of children in FRFF group went down (P ＜ 0.01);while the values of h1,h3,h4,h5,t,w,h3/h1 and h4/h1 of TRYF group fell (P ＜ 0.01);and those of h5,t and h5/h1 of children in FHFF group decreased (P ＜ 0.01).Compared with FQX group,h1 value of FRFF group increased (P ＜ 0.01),while the values of w,h3/h1,hs/h1 and w/t of FRFF group declined (P ＜ 0.01);and the h1 value of TSZF group boosted (P ＜ 0.01),while the value of w and h3/h1 of TRYF group decreased (P ＜ 0.01);and the h5/h1 value of FHFF group fell (P ＜ 0.01).In comparison with FRFF group,the values of t,w and h5/h1 of TSZF group went up (P ＜ 0.01),while the values of h1 and h3 of TRYF group declined (P ＜ 0.01).In comparison with TSZF group,the values of h3,h4,t and w of TRYF group went down (P ＜ 0.01),and the t value of FHFF group decreased (P ＜ 0.01).In conclusion,the pulse parameters of RRTI children can be recognized as objective indicators for TCM syndrome differentiations.

AIM:Chronic exposure to elevated levels of free fatty acids (FFAs) in type 2 diabetes patients is toxic to pancreatic β-cells.Thioredoxin (Trx)-interacting protein (TXNIP), an endogenous Trx-inhibiting protein, is up-regulated by glucose and is a critical mediator of hyperglycemia-induced β-cell apoptosis in diabetes.However, the effects of FFAs on TXNIP are unknown.In this experiment we observed the effect of palmitate on TXNIP expression in cultured INS-1 islet cells and the pathways involved were analyzed meanwhile.METHODS:After the full basis of preliminary experiment of incubating INS-1 cells with palmitate at different concentrations for different time, INS-1 islet cells were cultured with 0.5 mmol/L palmitate for 24 h.TXNIP expression, cell apoptosis, and expression of transcription factors related to TXNIP transcriptional regulation were determined.RESULTS:Compared with control group, the expression of TXNIP at mRNA and protein levels in palmitate group was significantly up-regulated (P<0.01).Cleaved caspase-3/caspase-3 ratio was increased in palmitate group (P<0.05), and the apoptosis of the INS-1 cells was also significantly increased (P<0.01).Palmitate enhanced the phosphorylation of nuclear factor-κB (NF-κB) (P<0.01), and the NF-κB inhibitors, PDTC and SN50, both blocked the palmitate-induced up-regulation of TXNIP expression.CONCLUSION:Saturated fatty acid palmitate enhances the expression of TXNIP.The mechanism of palmitate-induced TXNIP expression may be associa-ted with the increase in NF-κB phosphorylation.