Posterior capsular opacification (PCO),also known as after-cataract,is the most frequent complication and the primary cause for visual decrease after extracapsular cataract surgery.At present,there is no effective way to treat PCO,so more attentions are focused on preventive reseaching of PCO and treatment methods.Although a variety of studies have increased our understanding of the pathogenesis of PCO,the cellular mechanisms responsible for PCO are still unclear.Cultured capsular bag model in vitro could effectively simulate lens capsular membrane and cells survival environment after cataract extraction and IOL implantation.However,lens capsular bag cultivation with different methods have their own characreristics.The material source,preparation methods of capsular bag model,characreristics of materials which maintain capsular bag contours and its application in PCO were reviewed.

The expression of microRNAs (miRNAs) alters obviously in ovarian cancer.Through miRNA profile based on a microarray platform and further research on individual one,they are found to be closely related to pathogenesis,progression,recurrence,and drug resistance of ovarian cancer and have a good prospect in applying it in the early diagnosis,detection recurrence,prognosis and therapy of ovarian cancer.

Objective To study the relationship between static duration and bacteria reproduction of pure water from the RO purifier. Methods The original structure of RO purifier was modified by installing additional UV lights and ozone disinfector respectively. Bacteria reproduction in the effluent from RO purifier and modified RO purifier was detected after different static duration. Results The total count of bacteria in the fresh pure water was 20 cfu/ml which was up to the standard of pure drinking water CJ 94-1999. However the total count of bacteria in the pure water will be more than 50 cfu/ml after the pure water was stored inside the tank for six hours, which was not up to the standard of CJ 94-1999. Both ultraviolet rays and ozone could prolong the static duration of pure water, and the efficacy of ultraviolet rays was better than ozone. Conclusion UV light should be installed in RO purifier, the disinfected pure water by UV should be used in 16 hours after disinfection.

Aged ; Back ; Carcinoma, Medullary ; metabolism ; pathology ; Diagnosis, Differential ; Female ; Humans ; Melanoma ; metabolism ; pathology ; secondary ; surgery ; Melanoma-Specific Antigens ; metabolism ; S100 Proteins ; metabolism ; Skin Neoplasms ; metabolism ; pathology ; surgery ; Thyroid Neoplasms ; metabolism ; pathology ; secondary ; surgery

Objective To investigate the effect and mechanism of low-dose remifentanil(RF)on systemic artery tension of baby rabbit with septic shock.Methods Thirty-six systemic artery strips were prepared from 12 baby rabbits and randomly divided into 6 groups:control group,lipopoly-saccharide(LPS)group,RF group,LPS + RF group,NG-nitro-L-arginine(L-NNA)pretreated group,LPS + L-NNA pretreated group.Each group had six rings(n =6).The effect of low-dose RF on the systemic artery tension of baby rabbit with septic shock was observed with isolated vascular ring technique.The change was also obversed after L-NNA pretreatment.Results In control group:the relaxant rates after low-dose RF at the time point of 5 min,10 min,20 min were(18.48±3.96)％,(23.63±4.42)％,(28.33±3.73)％(P＜0.05),compared with NS group.After pretreatment with L-NNA,RF-related relaxant rates of systemic arteries decreased significantly to(8.15 ± 1.01)％,(13.08 ± 1.46)％,(18.54 ±2.94)％(P ＜ 0.05).In LPS group,low dose RF(4 μg/L)did not bring out any response to systemic arteries(P ＞0.05).The tension was not affected with pretreatment of L-NNA(P ＞ 0.05).Conclusion These results suggest that low dose RF has relaxant effect on systemic arteries of baby rabbits,and NO may be involved.Low dose RF has no relaxant effect on LPS-pretreated systemic arteries of baby rabbits,and it has no association with NO.

Objective To investigate the effects of cefoprazone/sulbactam(CPZ/Sul)and dexamethasone(DXM) on serum IL-8,IL-10 in SD rats with severe pneumonia due to Klebsiella pneumoniae.Methods A rat model of severe pneumonia was induced by intratra- cheal instillation of Klebsiella pneumoniae with a dose of 3.6×108CFU(0.3ml).60 male SD rats were random divided into control group,model group,cefoprazone/sulbactam(CF-L/Sul)group and cefoprazone/sulbactam plus dexamethasone(DXM+CPZ/Sul)group.15 rats in each group were then divided into three subgroups and 5rats in each subgroup were examined at the 6th,8th and 10th day,respectively.Arterial gas analysis.total WBC and PMN cell counts in BALF were examined.ELISA method was used to detect the level of IL-8 and IL-10 in serum.RESULTSIn model group,PaO2 were progressively decreased while total WBC and PMN cell counts in BALF were markedly increased.In CPZ/Sul group,all parameters were alleviated,but there were no significant difference of serum IL-8 and IL-10 between model group and CPZ/Sul group.In DXM+CPZ/SUl group,all parameters were significantly alleviated including serum IL-8 and IL-10.Conclu sions Severe pneumonia of rat due to Klebsiella pneumoniae was characterized by significant elevation of serum IL-8 and IL-10,which suggested the imbalance of inflammatory/anti-inflammatory mediators during the course of severe pneumonia.DXM codd markedly decrease the production of inflammatory mediators and slightly decrease the production of anti-inflammatory mediators,which could restore the balance and contribute to the relief of severe pneumonia.

Objective To explore the regulatory roles of butorphanol on isolated rabbit pulmonary arteries by lipopolysaccharide(LPS)pre-incubation and assay the protective effect of ketamine on clinic sep tic shock patients.Methods Vascular ring tension detection technique was applied to observe the effect of butorphanol on the normal or lipopolysaccharide incubated pulmonaryvascular.Results In normal group, the final concentration of butorphanol(0.1 μmol,1μmol,10μmol,100μmol)were used to make pretreat ment- phenylephrine(PE)pulmonary artery relaxation.In LPS group,the effect of vasodilation of butor phanol was enhanced.Conclusions LPS can enhance the effect of butorphanol on pulmonary artery relaxa tion reaction.And the direct effect of butorphanol on pulmonary arteries was vasodilation.

Objective To explore the diagnosis,treatment and nursing of the patients complicated with pneumocystis carinii pneumonia (PCP) after renal transplantation,and to enhance the treatment effect of the disease.Methods The treatment and nursing processes of 37 patients complicated with PCP after renal transplantation in department of organ transplantation were retrospectively analyzed in the First Affiliated Hospital of China Medical University from May 1992 to July 2011.And the key points during nursing work were summarized.Results Of the total 37 patients,10 patients occurred respiratory failure,they were all treated by noninvasive ventilation,among which 6 patients were converted to tracheal intubation or tracheotomy mechanical ventilation.5 patients appeared rising serum creatinine(Scr) during treatment,but recovered to normal level after discontinuing SMZco for 1 week.4 patients died unfortunately,and the others were cured and discharged.Conclusions The occurrence of PCP after renal transplantation is closely related to the immune state of the patients,thus the priority of treatment for PCP should be prevention.Once happened,nursing care in aid is particularly important,then nurses should encourage patients to cooperate with treatment,relieve symptoms after infection,and promote recovery as well.

The construction of nano-bionic drug delivery system based on cells or cellular components is a research hotspot of novel drug delivery systems at present. The nano-bionic drug delivery system can integrate the characteristics not only high drug loading and controlled release of nano-carriers, but also good biocompatibility, low immunogenicity and natural targeting from bionic components of cell, and it can also integrate with flexible morphology from living cells. Among them, nano-bionic drug delivery system based on macrophages possesses a good prospect of clinical application because of phagocytic function, inherent tendency, deep penetration ability and potential in cell therapy of macrophages in the treatment of tumors. Based on this, this paper reviews the drug loading strategies of nano-bionic drug delivery system based on macrophages and its application in tumor therapy, so as to provide reference for the development of novel drug delivery systems.

Objective To formulate paclitaxel loaded polymer nanoparticle and evaluate it's application in treatment of bladder.Methods Paclitaxel loaded Poly (lactide-co-glycolide) (PLGA) nanoparticles were formulated with microemulsion method,Polyvinyl alcohol(PVA) was used as surfactant.Transferrin (Tf) was used to modify the nanoparticles.The size,Z-potential,drug loading,drug release,cytotoxicity of bland nanoparticles and paclitaxel-loaded nanoparticles on bladder cancer cell line J-82 were measured.Results The size of nanoparticles was about 200 nm,Z-potential was-24 mV,drug loading was about 6.5％ (w/w),cumulative drug release showed two phase curve.The size of Tf modified nanoparticles was a little bigger than no modified nanoparticles.The Z-potential,drug loading,drug release was similar.Two kinds of blank nanoparticles showed no cytotoxicity on bladder cancer cell line J-82.However,both paclitaxel-loaded nanoparticles had significantly higher cytotoxicity on J-82 compared to paclitaxel solution.Conclusions PLGA nanoparticle is a promising drug delivery vehicle,which could significantly improve the anticancer effect of paclitaxel on bladder cancer.