Objective To study the clinical significance and characteristic of acute leukemia(AL) immunophenotype expression in children.Methods Immunofluoscence labeled with three and two color monoclonal antibodies directly were used to analyze the surface antigen and cytoplastic antigen.Cytoplastic antigen was detected by flow cytometry additionally.Results Specimens from 85 patients with childhood acute leukemia were detected.1.The undifferentiated phenotype account for 1 case(1.2%);Mixed leukemia 7 cases(8.2%);Acute myeloid leukemia 18 cases(21.2%);Acute lymphoid leukemia 59 cases(69.4%).The subtypes of acute lymphoid leukemia:(B-ALL) 50 cases(84.7%),T-ALL 6 cases(10.2%),T/B 3 cases(5.1%).2.Among 59 cases of acute lymphoid leukemia,there were 31 cases ALL expression myeloid-associated antigen;There was no difference on the clinical and prognosis between the group of myeloid-associated antigen positive and the negative group.3.Of 18 patients with AML,5 patients(27.8%) expressed lymphoid antigen,CD7 was the most commonly expressed(60%);The frequency of CD_(14) expression in childhood M4,M5 was 75%.CD_(13),CD_(33) presented in 83.3% and 94.4% cases of AML,respectively.4.Among 7 cases acute mixed leukemia,B/M 6 cases,TB/M 1 case.Five cases were misdiagnosed as ALL by FAB classification.Conclusions With the multi-color flow cytometry,every acute leukemia patient was diagnosed accurately,especially on acute mixed leukemia and cross-lineage expression leukemia.Those help us choice the best treatment protocol for childhood AL.

Diagnostic Errors ; Female ; Humans ; Maxillary Sinus ; pathology ; Melanoma ; diagnosis ; Middle Aged ; Paranasal Sinus Neoplasms ; diagnosis ; Polyps ; diagnosis ; pathology

Antibodies, Monoclonal, Humanized ; therapeutic use ; Bevacizumab ; Electrocoagulation ; methods ; Endoscopy ; Epistaxis ; therapy ; Hemostatic Techniques ; Humans ; Male ; Middle Aged ; Telangiectasia, Hereditary Hemorrhagic ; therapy

In order to compare the characteristics of ethano l production, a serie s respiratory deficient mutants (rho -) of Saccharomyces cerevisiae and the ir wil d parent strain (rho +) were assayed for ethanol yield, ethanol fermentation ki netics, ethanol tolerance, and activities of ethanol-dehydrogenase. The data i nd icated that: Though the average amount of ethanol produced by mutants rho - a nd by wild rho + strains were similar, some mutants produced a little more amount of ethanol than wild type. The kinetics curve of ethanol fermentation suggested t h at rho - mutants had a little higher ethanol fermenting speeds than wild type . Th e activity of ethanol-dehydrogenase per unit weight of protein of rho - mutan ts c ells were higher than that of wild strain rho +; the activity of ethanol-dehyd rogenase per unit volume of fermentation medium of rho + were higher than that rho - mutants,mainly due to the higher biomass of rho + were higher than that of rho - mutan ts. Some rho - mutants could tolerate higher ethanol concentration than wild rh o + stra in, but some not. If higher ethanol tolerance and higher ethanol yield rho - mu tants were used in fermenting process,higher ethanol yield would be expected,but some problems such as that rho - mutants grew slowly should be considered.

Aiming at the weakness of the medical graduate students' ideological and political education,we developed the new mode via investigation and surveying,allowing for the medical characteristics. We suggested that we should bring into full play the tutors and their groups function and excavate the connotation of groups’ morality education on the basis of the scientific research groups to develop the medical students' ideological and political education.

0 05) The Kappa value was 0 83 and intraobserver agreement was bet ter b etween SeO 2 and SpO 2 .ConclusionThe sensor sticked to earlobe is feasible and simple to monitor puls oxygen saturation continuousl y in children

Objective To observe neutrophil activation and protease imbalance in airway of children after foreign-body aspiration(FBA).Methods Bronchoalveolar lavage fluid(BALF) was obtained through fiberoptic bronchoscopy.The supernatant was assayed for the concentration of neutrophil elastase(NE) using enzyme-linked immunosorbent assay(ELISA) and elastase inhibition capacity(EIC)/free elastase activity using a colorimetric assay.The cell pellet were smeared for the expression of ?1-antitrypsin(?1-AT) using cellular immunohisto-chemistry and cytologic analysis by Wright-Giemsa stain.Results Percentage of neutrophil,concentration of NE and the expression of ?1-AT in BLAF group,pair group and pneumonia group significantly increased compared with that of control group(Pa0.05).The ratio of Lib NE in FBA,par and peumcria group was 30.3%,27.3% and 22.7%.Conclusions FBA induces similar airway neutrophil activation and protease imbalance to bacterial pneumonia.The contralateral airway showes the same change as the foreign body side.

China ; epidemiology ; Humans ; Occupational Diseases ; epidemiology

Brain ; surgery ; Cerebrospinal Fluid Rhinorrhea ; surgery ; Humans ; Postoperative Period

Objective To explore lymphocyte differentially expressed protein profile and its pathogenic significance in Huaihua Dong peoples with hypertension.Methods The lymphocyte protein profiles among 130 cases of blood specimen were detected and identified with matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF-MS) and Mascot search against protein database.Results The reproducible protein-maps of two-dimensional gel electrophoresis were obtained among three groups.Compared with Dong normal peoples,the protein disulfide isomerase-related protein 5 and heat shock protein 27 showed higher expressions in the Dong peoples with hypertension (P ＜ 0.01),but chain A,structure of haemoglobin in the deoxy quaternary state with ligand bound at the alpha haems is no statistical significance(P ＞0.05).Those three proteins were upregulated in Han peoples with hypertension (P ＜0.01).Conclusions The up-regulation of the protein disulfide isomerase-related protein 5 and heat shock protein 27 in the Dong and Han peoples with hypertension suggest that those two proteins might be associated with the occurrence and development of hypertension.