Objective To understand the health status of the staff at a vocational technology college and analysis the cause of health problems.Methods The data were analyzed and had been statistical processing according to the physical examination reports of a total of 707 Changzhou hygiene teaching staff from 2011 to 2013.Results The top ten diseases and total prevalence were as follows:Abnormal electrocardiogram 48.44％ (295/609),dyslipidemia 39.97％ (247/618),the male prostate hyperplasia 38.82％ (73/188),the female mammae lobule hyperplasia and breast lump 29.05％ (147/506),hypertension 27.44％ (152/554),the female uterine myoma 21.94％ (111/506),fatty liver 21.77％ (133/611),hepaticcysts 20.62％ (126/611),gallbladder polyps and cholecystitis18.66％ (114/611),elevated blood sugar 9.53％ (59/619).Dyslipidemia and abnormal of electrocardiogram were on the rise overall during the three years.Dyslipidemia was releated to sex and age,the number that plasma lipid of women aged from 55 to 64 reach appropriate level was lowest.Conclusions The faculty of vocational colleges have higher incidence of chronic diseases.Women group aged from 55 to 64 have highest incidence of dyslipidemia;more active,targeted measures need be taken.

Objective]To explore Professor YE Jin’s clinical experiences for dwarfism treatment based on spleen-insufficiency constitution related theory. [Methods]From the concept of dwarfism based on TCM, etiology and pathogenesis, key symptoms of spleen-insufficiency constitution and features medication to analyse the academic perspective and clinical experience of Professor YE Jin in the treatment of dwarfism, and list 1 proven case.[Results]Professor YE Jin thinks there’re 5 types constitutions of dwarfism, and attaches importance to adjust the unbalanced constitution of the patients. She thinks spleen-insufficiency constitution is basis of the other four types. She does well in enlivening and invigorating the spleen and activating spleen-energy.[Conclusion] Professor YE Jin has unique clinical experience for dwarfism treatment based on spleen-insufficiency constitution related theory, with significant effect.

OBJECTIVE To evaluate the efficacy of combined therapy on chronic rhinosinusitis(CRS). METHODS Thirty cases with CRS in stage Ⅱ-2 were treated by FESS. At 1,3,6-month after operation,endoscopy and nasal mucosa biopsy were performed and the clinic symptoms were reassessed. RESULTS Although fine mucosal epithelization was found in all cases at 6 month after operation,mild edema of mucosa and muciform secretion still existed in 10 cases. Mucociliary transport rate (MTR) was 3.63?1.74 mm/min before operation,and was 6.27?1.31 mm/min at 6-month after operation. Compared with that before operation,the MTR was significantly improved whereas it had not rehabilitated to normal status. The decrease of inflammatory cells and alleviation of submucosal edema were observed in the mucosa of maxillary sinus. Nevertheless,the number of goblet cells and submucosa glands did not improve significantly. The ultrastructure of the mucosa was not recovered completely at 6 month after operation. CONCLUSION With combined therapy on CRS for 6 months, clinical cure can be achieved,but mucosal inflammatory reaction is still existed.

[Objective]The paper was designed to introduce the Professor YE Jin's personal clinical experience which can provide an effective means for other pediatrician.[Method]By introducing the Professor YE Jin's personal clinical experience,we summarize the effective therapies about the infantile recurrent abdominal pain(RAP). [Result]According to the Professor YE Jin, the pathomechanism of RAP includes mainly cold stagnation, the indigestion, parasite and Qi stagnancy. RAP caused bycoldcan be treated from warming the middle-energizer and fending off the cold,RAP resulting from retention of food can be cured by removing the retention and invigorating the stomach, RAP incurred by ascaris can be cured by relieving ascariasis,expelling parasite and purgation simultaneously,and RAP resulting from Qi stagnation can be cured by soothing liver, regulating qi activity, rather than relieving pain merely. Based on the above-mentioned theory,she tends to use Liquorice and prefers Paeonia a lot in curing the disease, that is always effective in clinic as a result.The recurrence rate is also lower.One case was provided for verification.[Conclusion]Professor YE Jin thinks that warming the middle-energizer and fending off the cold, removing the retention and invigorating the stomach, the RAP can be improved the curative effect,and that can provide another idea for other clinicians.

[Objective]This paper mainly introduced Professor YE Jin 's experience in treating girls with precocious puberty from liver syndrome differentiation and characteristics of prescription medication.[Methods] On account of three main symptoms of girls with precocious puberty at first visit, including thelarche, leukorrhea and menses, we explore the relation between liver and the causes of this disease from the viscera-state doctrine as well as the meridian theory. Then introduce Professor YE Jin's methods and empirical medication in treating girls with precocious puberty based on liver. In order to analyse her experience, a clinical case was reported. [Results]Professor YE holds that the pathogenesis of girl 's precociouss puberty closely related to liver. The deficiency of liver-yin and kidney-yin is the basic pathogenesis of precocious puberty, which is often accompanied with liver qi depression or even liver qi depression transforming into fire. On the basis of nourishing yin and purging fire, Professor YE emphasizes treating precocious puberty from liver. In practical terms, flexibly applying the ideas of softening, soothing and clearing liver can effectively improve the clinical symptoms. She makes the selection and compatibility of Chinese medicine comforming to the characteristics of children 's physiology and pathology and values the protection of vital energy. The reported case specifically demonstrates treating a girl 's thelarche from liver syndrome differentiation as well as the empiric therapy. [Conclusion]Professor YE Jin's experience in treating girls with precocious puberty is well worth learning for its significant curative effect.

This paper outlines some molecular marking technology based on rDNA sequences and several DNA fingerprinting technology (RAPD, ARDRA, AFLP, REP/ERIC-PCR) used in classification, identifica-tion and diversity research in lactic acid bacteria. The principles, methods and progress in recent years of these technologies were also introduced. At the same time, this paper also compares the advantages and dis-advantages of these methods. People should choose suitable method according to their purposes.

Objective To summarize and analyze the emergency management for acute renal failure of solitary kidney induced by upper urinary calculi. Methods A total of 78 cases of acute renal failure of solitary kidney induced by upper urinary calculi were included.The mean course was 2.8 days (range,1 to 8 days).The time from onset to emergency management was within 72 hours in 68 out of the 78 cases.10 cases were treated with percutaneous nephrostomy drainage (PCN) under the guidance of B-ultrasound;25 cases with emergent open surgery;12 cases with indwelling ureteral stent retrogradely;and 31 cases were treated with transurethral ureteroscopy ballast lithotrity. Results One died of bleeding and infection after operation.In 65 cases treated with in 72 hours after obstruction, the serum Cr level decreased from 202～885 ?mol/L to 58～130 ?mol/L;the serum BUN level decreased from 9.8～42.4 mmol/L to 2.8～7.0 mmol/L after urgent treatment during a period of 3 to 12 days,and the renal functions recovered. Conclusions The principles of management for this acute condition should be diagnosing early and promptly,releasing obstruction as soon as possible,preventing complications effectively and reserving renal functions.KUB and B-ultrasonography are the first choice of diagnostic measures.Transurethral ureteroscopy ballast lithotrity can be recommended as the first choice of emergent treatment.

Objective To study the genetic toxicity of carbon tetrachloride for male mice and assess the potential genetic toxic effect on mankind. Methods Micronucleus test and sperm malformation test were used respectively to determine the change of micronucleus cell frequencies (MNCF) and the rate of sperm deformity of germ cell induced by carbon tetrachloride in male mice. Carbon tetrachloride was given through intraperitoneal injection at does of 5,15,25 mg/kg respectively for 24 h and 48 h, the rates of micronucleus in the bone marrow cell and sperm deformity of germ cell of male mice were investigated by counting the number of micronucleus cells per 5 000 polychromatic erythrocyte (PCE) in bone marrow and sperm deformity cells per 5 000 germ cells from male mice. Results The micronucleus rates of PCE in bone marrow and sperms deformity frequencies in carbon tetrachloride treated groups were much higher than the control group (P

Objective To investigate the efficacy,side effects and cosmetic effects of breast-conserving operation combined with radiotherapy for patients with breast cancer. Methods Forty-three patients with early stage of breast cancer(n=30 for stage I and n=13 for stage II) were performed extended resection plus axillary lymph node dissection or quadrant resection plus axillary lymph node dissection.After operation,tangential field irradiation was conducted with 50 Gy of X ray,tumor bed was irradiated with additional 10 Gy of electron beam,and supraclavicular field irradiation was also administered with 30 Gy of X ray and 22 Gy of electron beam. Results There was no recurrence during the follow up for 14 to 50 months.The acute side effects of radiotherapy included breast complaints(n=10,grade Ⅰ,23.26%),skin erythema and hyperpigmentation(n=19,grade Ⅰ,44.19%),moist desquamation(n=5,grade Ⅱ,11.63%) and radiation esophagitis(n=4,grade Ⅰ,9.30%),while one patient experienced radiation esophagitis one month after radiotherapy.No such complications as skin fibrosis and necrosis,angiotelectasis,breast and upper extremity edema occurred.The short-term cosmetic effects were satisfactory to 93.02% of all the patients. Conclusion For early stage of breast cancer(stage I and stage Ⅱ),breast-conserving operation combined with radiotherapy may yield satisfactory survival rate and short-term cosmetic effects,as well as low incidences of side effects.