Aim To investigate the effects of xy9902 on the proliferation and differentiation in MC3T3-E1 cells and its mechanism.Methods Effects of xy9902 on the proliferation and differentiation of MC3T3-E1 cells was determined by MTT and ALP activity measurement.Competitive estrogen-receptor binding assay was used to investigate the affinity of xy9902 to ER.Results xy9902 induced cell proliferation and increased alkaline phosphatase(ALP) activity of MC3T3-E1 cells.Antiestrogen tamoxifen eliminated the stimulation of proliferation and ALP activity of MC3T3-E1 induced by xy9902.The relative binding affinity of xy9902 for ER? and ER? showed an IC_(50)of 8.45?10~(-6) mol?L~(-1) and 1.66?10~(-6) mol?L~(-1) respectively.Conclusion xy9902 directly stimulates cell proliferation and differentiation of MC3T3-E1 cells through ligand-receptor binding,which indicated that xy9902 behaves as an estrogen agonist in MC3T3-E1 cells.

The differential diagnosis of pancreatic head mass has been clinical difficulties.Endoscopic ultrasonography is now recognized as one of the sensitive and effective methods for the diagnosis of pancreatic diseases.This paper discusses the diagnostic performance of endo-scopic ultrasonography for various common types of pancreatic head mass,as well as the radiological features of this disease,reviews the di-agnostic value and safety of endoscopic ultrasonography-guided fine needle aspiration for pancreatic head mass,and briefly introduces the advances in the application of additional new techniques of endoscopic ultrasonography for this disease.Finally,it is pointed out that endo-scopic ultrasonography has a high diagnostic value for pancreatic head mass.

With the development of endoscopic techniques,the recent emergence of additional techniques of endoscopic ultrasound (EUS) have provided a novel means for the diagnosis and treatment of pancreatic tumors,which has received more and more attention and recogni-tion from around the world.This article reviews the progress in additional new techniques of EUS in the treatment of common pancreatic tumors.Finally,we can conclude that the additional techniques of EUS have high therapeutic values for pancreatic tumors.

Objective To investigate the influences of Rhodospirillaceae on immune function of mice.Methods Four tests were performed in mice after 4 w medication with different doses of Rhodospirillaceae ig,including:1.The transformation of spleen lymphocyte(MTT method);2.The phagocytosis of macrophagocyte(semi-vivo method);3.The value of HC50; 4.The activity of NK cell(LDH method).Results1.The transformation of spleen lymphocyte;2.The phagocytosis of macrophagocyte,the Value of HC50 and the activity of NK cell in test groups were all raised,the differences were significant and very significant when compared with the control group.Couclusions Rhodospirillaceae could enhance the cellular and humoral immunity of mice.

Objective To research the way ro deal with the acute laryngeal damage.Methods Data of 23 cases with acute laryngeal damage were analyzed.Results Among 23 cases,there were 6 cases who received non-surgical treatment and their phonation were normal except one case who had mild hoarseness.Among 17 cases who had been performed operation,13 cases had normal phonation after operation.4 cases still had roughness of voice.There were 15 cases with normal respiration but two cases with larynostenosis and difficulty in decannulation.And these two cases were decannulated respectively after 2 months and 3 years after operation.15 cases made of 80% in all were cured and decannulated within 1 month.Conclusion Acute laryngeal damage patients should be diagnosed and treated as early as possible according to the damage degree so as to recover the laryngeal and tracheals function and prevent the sequelae and increase the cure rate.

Objective To study the clinical features,diagnosis and management of nose and nasal sinusen fungus.Methods Different manner of surgical procedure was taken according to the areas and degree of pathological changes.The patients with noninvasive fungus were treated with radical maxillary sinusotomy(cald-well-Luc operation) or endoscopic sinus surgery.The patients with invasive form were undergone by the sinusotomy of enlarging areas,and combined with a postoperatively antifungal therapy.Results Twenty-six patients were involved in noninvasive fungus,and 25 cases in them were cured in single surgery through different manner.Nine patients were in invasive one,and 7 in them were also cured by single surgery in dfferent manner.One patient with invasivi fungus died 5 months postoperatively for extensive area and noneffective treatment although four surgical excisions were done.Conclusion CT scan is very important in their diagnosis,and the histopathology leads to identification of the fungus.Surgical removal is a radical kind of treatment.It is essential to exercise and open entirely the involved sinus cavity whatever type,and to enlarge areas for the extensive invasive fungus.

Hepatitis B ; pathology ; therapy ; Hepatitis B Surface Antigens ; Hepatitis B virus ; Humans

Drug Resistance, Viral ; genetics ; Hepatitis B virus ; drug effects ; genetics ; Mutation

Congresses as Topic ; Otorhinolaryngologic Surgical Procedures ; Rhinitis ; surgery ; Skull Base ; surgery

Cofactor engineering, a vital part of metabolism engineering, changes the redox cofactor regeneration approach. Its main goal is to rebuild the components of metabolic products. The bioconversion of xylose for the production of ethanol is being studied intensively because ethanol is an alternative energy source and a potential liquid fuel. Saccharomyces cerevisiae has been traditionally used in producing ethanol from fermentable sugars but it cannot utilize xylose, only its isomer xylulose. Introduction of the xylose fermentation pathway from Pichia stipitis into S. cerevisiae enables xylose utilization in recombinant S. cerevisiae, but the ethanol yields of xylose fermentation with recombinant S. cerevisiae has been low and large amounts of the byproduct xylitol are produced. The major reason is that the catabolism of xylose with the fungal pathway leads an imbalance of redox cofactor. The process of the catabolism of xylose requires NADPH and NAD~+, both of which have to be regenerated in separated processes. More and more attention has therefore focused on the redox cofactor balance in S. cerevisia. The research progress of cofactor engineering to solve the imbalance of redox cofactor in xylose metabolism recombinant S. cerevisiae was introduced. This included expression of transhydrogenase, increasing the utilization of NADPH, and achieving the anaerobic reoxidation of NADH. Reversing the cofactor specificity of enzymes is another effective way.