1.Recurrent implantation failure: gamete and embryo factors.
National Journal of Andrology 2014;20(6):544-547
Despite the immense achievement in the field of IVF in recent years, many patients still suffer from recurrent implantation failure. Therefore, much attention has been drawn to its etiology and treatment. Chromosomal abnormality, sperm DNA damage, zona hardening, and inappropriate culture conditions are important factors that lead to recurrent implantation failure. Results of studies suggest that preimplantation genetic screening does not improve the rate of implantation or live birth. Comparative genomic hybridization array and single nucleotide polymorphism array could offer a more comprehensive screening of chromosomes. Assisted hatching may help to solve the problem of zona hardening in some situations. Co-culture and blastocyst transfer could be conducive to the improvement of the rates of implantation and pregnancy. Cytoplasmic transfer may give a solution to ooplasmic composition anomalies.
Chromosome Aberrations
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Coculture Techniques
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Embryo Implantation
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Embryo Transfer
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Female
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Fertilization in Vitro
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Germ Cells
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Humans
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Male
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Pregnancy
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Preimplantation Diagnosis
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Treatment Failure
2.Cost analysis and perspective of clinical inhaled nitric oxide
Hui HU ; Haiyan LIANG ; Heli WANG ; Jin LI
Chinese Medical Equipment Journal 2004;0(07):-
Inhaled nitric oxide has been identified as a potent method for curing acute respiratory distress syndrome,high brood pressure and some illnesses related to the lung,but the high cost limits its application.The present study investigates the reason of the high cost of inhaled nitric oxide in the current application,and gives some advices on solving them by using pulsed arc discharge in clean air,reducing the price of nitrogen oxides analyzer and being compatible with the present ventilators.The perspective of inhaled nitric oxide was viewed as well.
3.Hospital Infection after Liver Transplantation:An Epidemiology Study
Xianping WANG ; Huining YANG ; Jin GAO ; Hui LIANG ; Li CAO
Chinese Journal of Nosocomiology 2006;0(08):-
OBJECTIVE To study the characteristic of epidemiology of hospital infection among recipients after liver transplantation and to probe into its risk factors.METHODS Totally 193 cases were investigated.The infection group was compared with the non-infection group.Descriptive statistics and risk factor analysis were performed with SPSS11.0.RESULTS Forty nine cases took place infection.The infection rate was 25.4% and the death rate was 20.41%.15 cases(30.61%) happened 2 or 3 time infections.Mid-time of infection was 14d.Twenty six case(30.61%) infection occurred within 4 weeks and 25 cases(51.02%) were on June and July.Pulmonary infection(32.84%),wound infection(31.34%) and abdominal cavity infection(23.90%) were ranked in front.A total of 693 strains were isolated,and Gram-negative(G-) bacilli were 42.14%,Gram-positive(G+)cocci 35.30% and fungi were 22.66%.The three risk factors were obviously correlated with the infection(P
4.The jaw multiple tumor-like lesions as the first symptom of the parathyroid adenoma:a case report.
Jin-hui LIANG ; Hai-lin LUO ; Li LI
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(12):1048-1048
Adolescent
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Female
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Humans
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Jaw
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pathology
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Parathyroid Neoplasms
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complications
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pathology
5.Analyze Antibacterial Resistance Character of Stenotrophomonas maltophilia in ICU
Xian-Ping WANG ; Jin GAO ; Hui LIANG ; Li CAO ;
Microbiology 1992;0(05):-
Stenotrophomonas maltophilia is among eight strain of broad resistance to antibacterial. It has been pay attention to global students, and is very difficulty in clinic treatment. Infection of Stenotrophomonas maltophilia is more familiar in ICU. Its test rate is 13.45%(62/461) in the study .The resistance character: IPM﹑CZO﹑CRO﹑AMP and MNO is highest(resistive rate 100%). ATM、GEN 、 KAN 、MEM 、CTX and PIP is much high(≧86.5%), LVX﹑GAT and FEP is high (=50%).Conclusion: Stenotrophomonas maltophilia should be monitored in the hospital. Used of antibacterial must be rational. Severe disinfection and isolation should been put in practice to the infected patients. CSL is very active antibacterial (active rate 90.6%) against Stenotrophomonas maltophilia in the study, and ought be selected headmost in treated its infection.
6.Study of production of sesquiterpenes of Aquilaria senensis stimulated by Lasiodiplodia theobromae.
Xiao-Min HAN ; Liang LIANG ; Zheng ZHANG ; Xiu-Jin LI ; Yun YANG ; Hui MENG ; Zhi-Hui GAO ; Yan-Hong XU
China Journal of Chinese Materia Medica 2014;39(2):192-196
To investigate the mechanism of agarwood formation in Aquilaria sinensis induced by Lasiodiplodia theobromae, the fermentation liquor of L. theobromae was analyzed qualitatively and quantitatively by gas chromatography-mass spectrometry (GC-MS). JAs were detected in the fermentation liquor. The effect of the fermentation liquor on the abundance of sesquiterpenes in the callus of A. sinensis was analyzed by solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS). And the fermentation liquor stimulated alpha-guaiene, alpha-humulene and delta-guaiene biosynthesis in calli. It was inferred that L. theobromae produced JAs, which resulted in a significant increase of sesquiterpenes in A. sinensis.
Ascomycota
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physiology
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Fermentation
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Sesquiterpenes
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metabolism
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Thymelaeaceae
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metabolism
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microbiology
7.In vitro effect of total flavones of Fructus Chorspondiatis on expression of collagen type I and type III mRNA and protein of cultured rat cardiac fibroblasts.
Jun-Ping BAO ; Ming JIN ; Yu-Min YANG ; Xiao-Hui GAO ; Liang SHU ; Hui-Hui XING ; Lei JIA
Acta Pharmaceutica Sinica 2014;49(1):136-141
This study aims to investigate the effect of total flavones of Fructus Chorspondiatis (TFFC) on the mRNA and protein expression of collagen type I and III of rat cardiac fibroblasts (CFs) induced by angiotensin II (Ang II), and explore its anti-myocardial fibrosis molecular mechanism. Neonatal rat CFs were prepared from Sprague-Dawley rats (1-3 d after birth). The expression of collagen type I and III mRNA and protein were measured by RT-PCR and Western blotting, respectively. The study showed that stimulation of neonatal rat CFs with 100 nmol.L-1 of Ang II for 72 h resulted in a significant increase of the expression of collagen type I and III mRNA and protein. The changes on the expression level were blocked by TFFC. The results demonstrated that TFFC can inhibit myocardial fibrosis induced by Ang II in rats, which is probably associated with the collagen type I and III mRNA and protein levels up-regulated by Ang II, and TFFC was shown to decrease the expression levels of collagen type I and III mRNA and protein.
Anacardiaceae
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chemistry
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Angiotensin II
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pharmacology
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Animals
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Animals, Newborn
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Cells, Cultured
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Collagen Type I
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genetics
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metabolism
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Collagen Type III
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genetics
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metabolism
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Dose-Response Relationship, Drug
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Drugs, Chinese Herbal
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administration & dosage
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isolation & purification
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pharmacology
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Fibroblasts
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cytology
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metabolism
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Flavones
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administration & dosage
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isolation & purification
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pharmacology
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Fruit
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chemistry
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Myocardium
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cytology
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metabolism
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Plants, Medicinal
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chemistry
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RNA, Messenger
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metabolism
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Rats
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Rats, Sprague-Dawley
8.Transsphenoidal microsurgery of non-adenomas disease intrasellar region:a report of 23 cases
Wen-Sheng LI ; De-Jin SHI ; Hui WANG ; Ying GUO ; Chao-Feng LIANG ; JIN
Chinese Journal of Microsurgery 2006;0(06):-
Objective To evaluate the overall effect of transsphenoidal microsuegery for 23 patients with non-adenomas disease intrasellar region.Methods A toal of 23 patients with non-adenomas disease in- trasellar region,7 cases of Rathke's cleft cyst,3 cases of craniopharyngiomas,2 cases of meningiomas,2 ca- ses of pituitary tuberculous granuloma,5 cases of pituitary abscess,2 cases of empty sella,2 cases of chord- mas,were treated via sublabio-septo-sphenoidal microsurgery.Results There were among the 23 patients, groos total removal of the disease were achieved in 14 cases,subtotal removal in 7 cases,and partial removal in remain 2 cases.Diminished visual activity and visual field defects were improved in 13 cases.Menstrual disorder in all female cases and sexual disturbance in male were improved.There was no death in group. Conclusion Microsurgical technique via transsphenoidal approach is a safe and effective one for the treatment of patients with non-adenomas disease intrasellar region.
9.Clinical effect of a combined ultrasound and pneumatic lithotripter on intrahepatic and extrahepatic bile duct stones
Fangxing PENG ; Sanying YUAN ; Liang LUO ; Hui CHEN ; Chen WU ; Jin CHEN
Chinese Journal of Current Advances in General Surgery 2017;20(6):451-453,457
Objective:The application of a combined ultrasound and pneumatic lithotripter for cholelithiasis was investigated.Methods:A total of 59 cases of cholelithiasis diagnosed by ultrasonography and CT were included in the study.All the patients had surgical treatment from January 2013 to December 2015,in which 30 patients were treated with a combined ultrasound and pneumatic lithotripter protocol (EMS group),and 29 patients were treated with traditional choledochoscope protocol(control group).All the patients were followed up for 3 months postoperation.The outcomes of patients in EMS group and control group were compared.Results:The postoperative stone free rate after the first operation is higher in EMS group than in control group(P<0.05).The operation time and total lengths of hospital stay were shorter in EMS group than in control group(P<0.05).There was no significant difference in intraoperative hematobilia rate,intraoperative bile duct injury rate,postoperative biliary tract infection rate,hepatic biochemical index and mortality between two groups(P>0.05).Conclusion:The combined ultrasound and pneumatic lithotripter is useful for treatment of cholelithiasis.
10.Effects of Qingrehuayuziyin Fang on lymphocyte apoptosis and expression of Fas and FasL in peripheral blood in the patients with systemic lupus erythematosus
Wei LIANG ; Lin JIN ; Ling GONG ; Liling ZHANG ; Hui LIAO ; Ying CAI
Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(09):-
AIM: To investigate the effect of Qingrehuayuziyin Fang on lymphocyte apoptosis and expression of Fas and FasL in peripheral blood in the patients with systemic lupus erythematosus(SLE).METHODS: Eighty patients with SLE were selected and divided randomly into two groups,40 in the therapy group and 40 in control group respectively.Patients were get the treatment of Qingrehuayuziyin Fang combined hormone(prednisone) as therapy group and get hormone treating only as control group.After treating three months,lymphocyte were collected to be detected the rate of apoptosis by flow cytometer.Meanwhile,the expression of Fas and FasL were analyzed by RT-PCR.RESULTS: After three months treating,the rate of lymphocyte apoptosis was decreased significantly in therapy group compared with that in control group(P