The amount of ~3H-TdR incorporation into D?A of FRTL5 cells was expressed as CPM/?gD?A. THe responsibity of FRTL5 cells to stimulators such as TSH was expressed as % against thJ control CPM/?gDNA of the experimental group divided by that of the control. The responsibilities of FRTL5 cells which were recovered after being fr-o zen under-180℃ for a long time, to TSH 200?g/ml were 2741% and 3028% respectively, and the responsibilities of cells cultured in TSH media continuously for a long time, to TSH 200?u/ml and 2000?u/ml were 736% and 719% respectively. There was statistically significant differences between the two groups of the cells mentioned above. This showed that freezing FRTL5 Cells for a long time Such as 2 months was able to recover the decreased responsibility of the Cells due to a long continuous TSH culture.

Objective To investigate the serum superoxide dismutase( SOD) ,malondialdehyde ( MDA) , and nitric oxide( NO) level in generalized anxiety disorder ( GAD) patients for exploring the importance of oxida?tive stress in etiology of GAD. Methods 42 cases of first episode patients with GAD ( GAD group) and 42 cases of health ( control group) matched with age and gender were included . Serum levels of SOD,MDA ,and NO were tested to analyze for a control study.Results GAD patients had significantly higher levels of SOD,MAD and NO than health controls ((858.09±137.32)×102 U/L vs (745.40±119.19) ×102 U/L;(10.92±3.42)mmol/L vs (7.52±2.32)mmol/L;(74.32±12.34) μmol/L vs(65.22±14.29) μmol/L), t=4.036,5.368,3.297;P=0.000, 0.000,0.003) . A positive relationship between SOD and total score of Hamilton Anxiety Scale( HAMA ) ,psychotic anxiety factor of HAMA,or somatic anxiety factor of HAMA was found in GAD group ( r=0.331,0.370,0.318;P=0.029,0.016,0.040).The level of MAD correlated with total score or psychotic anxiety factor of HAMA( r=0.311, 0.320;P=0.042,0.039).Conclusion It is suggested that the dysfunction of oxidative stress may play a role in pathogenesis of generalized anxiety disorder.