2.Influence of doxorubicin chemotherapy on the expression of epithelial growth factor in the Balb/c mouse hepatocarcinoma
Chunbao GUO ; Feng CHEN ; Xianqing JIN
China Oncology 2001;0(03):-
Purpose:To investigate the influence of doxorubicin chemotherapy on overexpression of epithelial growth factor. Methods:Cancer animal model was made and treated with doxorubicin. The concentration of EGF protein in Plasma and mRNA in cancer tissue were detected by ELISA and Real-time quantitative RT-PCR respectively and the correlation of this parameter was evaluated. Results:The plasma concentrations of EGF expression were 3X Group 1.19?0.42 pg/ml, 1X Group 1.61?0.51 pg/ml, control group 2.13?0.68 pg/ml, and normal 0.91?0.33 pg/ml respectively. The concentration of EGF were analysed by ANOVA q test. The median concentrations of mRNA were 3X Group 1.6?10 3 copies/ml, 1X Group 8.5?10 4 copies/ml, control 4?10 5 copies/ml, and normal 2.5?10 2 copies/ml respectively, and were analysed by rank-sum test. It was shown that the control group had higher EGF and EGFR expression level than those treated with doxorubicin. EGF and EGFR expression of 3X Group were lower than that of 1X Group but remained higher than that of normal (P
4.Pharmacological intervention for contact granuloma of the larynx.
Jin-Rang LI ; Hong-Guang GUO ; Xi CHEN
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(5):387-388
Adult
;
Aged
;
Aged, 80 and over
;
Granuloma
;
drug therapy
;
Humans
;
Laryngeal Diseases
;
drug therapy
;
Male
;
Middle Aged
5.Relationship between endometrial blood flow measured by three-dimensional power Doppler ultrasound and pregnancy for women who received the treatment of in vitro fertilization and embryo transfer
Jin CHEN ; Lidan HAO ; Hua SHI ; Ruiqiang GUO
Chinese Journal of Ultrasonography 2011;20(10):861-863
ObjectiveTo evaluate whether endometrial blood flow measured by three-dimensional power Doppler ultrasound can predict pregnacy after the treatment of in vitro fertilization and embryo transfer (IVF-ET).MethodsNinty women who received the treatment of IVF-ET examined by threedimensional power Doppler imaging at the day of inject human chorionic gonadotropin(HCG) were enrolled.All patients were divided into pregnancy group (43 cases,include biochemical pregnancy and clinical pregnancy ) and non-pregnancy group(47 cases).The diagnostic value of parameters derived from threedimensional power Doppler imaging was evaluated by using the receiver operating characteristic (ROC)curve.ResultsDifferences of these parameters,including vascularization index (VI),flow index(Fl) and vascularization flow index (VFI)were considered statistically significant between two groups,the parameters of uterine artery and endometrial volume had no significant differences between two groups.The area under ROC curve of VI,FI and VFI were 0.880,0.713 and 0.646,respectively.Among these parameters,VI had the highest diagnostic value for discriminating between benign and malignant endometrium,the cut-off value of which was 0.733 with a sensitivity of 84.2%.Conclusions Threedimensional power Doppler ultrasound was useful for evaluating endometrial blood flow in IVF-ET cycles.Endometrial blood flow parameters may be useful predictors for pregnancy.
7.Reasons for encounter and diagnosis of pediatric patients at general practice clinic affiliated to a tertiary hospital
Wenjuan HUANG ; Zhonghua ZHOU ; Jingzhu GUO ; Shiyin CHEN ; Shihong JIN
Chinese Journal of General Practitioners 2013;(7):556-558
This study reviewed the reasons for encounter (RFE),diagnoses and the diagnostic categories of patients aged 0-14 y in general practice clinic affiliated to a tertiary hospital from June 2011 to May 2012.The results showed that the most frequent RFE was cough,at a rate of 29.6 per 100 encounters;the second one was fever (21.4%) and the third was nasal problems (9.5%).Medical examinations,skin rashes and follow-up encounters occurred at equal rates (5.2%).The most frequent diagnosis was acute upper respiratory tract infection (41%).Medical examination (6.3%),other viral diseases (5.1%),pneumonia (3.6%) and no detected disease (3.6%) were also common diagnoses.The diagnostic categories included all systems,and the problems of the respiratory system (56.4%) were most common.The results suggest that pediatric care comprises a very important part of primary care.
8.Silencing IDH-2 gene by siRNA-IDH-2 inhibits human small cell lung car-cinoma growth
Jianhong LU ; Guojun CHEN ; Changlin DONG ; Shaowen GUO ; Yijun JIN
Chinese Journal of Pathophysiology 2014;(8):1384-1387
[ABSTRACT]AIM:Toinvestigatetheeffectofsilencingisocitratedehydrogenase2(IDH-2)genebysmallinter-fering RNA (siRNA) on the biological characteristics of human small cell lung cancer cell line NCI -H446.METHODS:IDH-2 expression was knocked down in human small cell lung cancer cell line NCI -H446 by siRNA-IDH-2.The expression level of IDH-2 was determined by real-time PCR and Western blotting .The cell proliferation was measured by CCK-8 as-say , the protein expression of MAPK p 42 was detected by Western blotting , and the cell cycle was analyzed by flow cytome-try.The migration was observed using Transwell cell migration system .BALB/c nude mice were subcutaneously injected on the back with NCI-H446 cells transfected with siRNA-IDH-2/negative control siRNA or non-transfected cells to study the tumor growth .RESULTS:siRNA-IDH-2 remarkably down-regulated the expression of IDH-2 and MAPK p42 in the NCI-H446 cells.siRNA-IDH-2 inhibited both the proliferation and migration abilities of NCI-H446 cells, and the cell cycle was arrested in S phase as compared with negative control group .Additionally, the volume of xenograft tumors in siRNA-IDH-2 group was significantly decreased as compared with control group .CONCLUSION:siRNA-IDH-2 down-regulates the expres-sion of IDH-2 in NCI-H446 cells, reduces the cell migration efficiency and inhibits the tumor growth in vitro and in vivo.
9.Metabolic Flux Analysis of L-Arginine Fermentation in Corynebacterium glutamicum
Jin-Wei ZHU ; Qing-Shan CHEN ; Wei-Guo ZHANG ;
Microbiology 1992;0(03):-
In this paper, metabolic networks of the Corynebacterium glutamicum GWY020 and the two de-rivatives carrying additional mutations HUI821and GUI089 were established and modified. The concentra-tions of extra-cellular metabolites were determined under sub-steady-state (50 h~52 h) of the batch culture. The metabolic flux distribution maps of the three strains were obtained, compared and analyzed. These re-sults indicate that the introduction of analog supersensitive marker or analog resistant marker skew the metabolic flux towards the formation of L-Arginine. This study revealed the usefulness of the metabolic flux analysis as a tool for verification of existing production strains. The analysis may play an important role in helping us to rationally re-design metabolism for further improvement of fermentation process.
10.Experimental studies on microwave-assisted extraction of baicalin from root of Scutellariae baicalensis
Zhenku GUO ; Qinhan JIN ; Guoqiang FAN ; Yuping DUAN ; Chen QIN ;
Chinese Traditional and Herbal Drugs 1994;0(11):-
Object To study the microwave assisted procedure for the extraction of baicalin from root of Scutellariae baicalensis Georgi Methods An MSP 100D domestically made microwave sample preparation system with a maximum power of 850 watts was used. The effects of solvents, pressure/temperature of solvents and microwave radiation time on the yield of baicalin were studied by orthogonal experimental design. Results The optimal experimental conditions were extraction at 70% of microwave power with 30 times of 35% ethanol at a constant heating pressure/temperature of 0.15 MPa for 30 s. Conclusion The microwave assisted extraction not only takes a shorter time with better parallel results, but also gave an increased yield of about 10% as compared with ultrasonic extraction.