1.Effect of Shenxiong injection on inflammation injury of ischemia-reperfusion injury rats.
Hui-fen ZHOU ; Jin-chao AL ; Hai-tong WAN ; Yu HE ; Yu-yan ZHANG ; Tao ZHAO ; Wei FU ; Jie-hong YANG
China Journal of Chinese Materia Medica 2015;40(12):2408-2412
To investigate the effect of Shenxiong injection on the inflammation injury of ischemia-reperfusion injury senile rats. Totally 84 Sprague-Dawley (SD) rats were randomly divided into six groups: the sham operation group, the model group, the Nimodipine group and the Shenxiong injection(low, middle, and high dosage) groups. The rat cerebral ischemia-reperfusion model was established through intraperitoneal injection for 3 d and middle cerebral artery occlusion (MCAO). Ater the reperfusion for 24 h, efforts were made to give neurological score, collect brains for TTC staining, detect tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) content in serum by enzyme-linked immunosorbent assay (ELISA) method and measure IL-1β, ICAM-1 and MMP-9 mRNA expressions in hippocampal area by Real-time PCR (RT-PCR). According to the results, Shenxiong injection could decrease the cerebral infarction volume, greatly improved the neurological function and reduce IL-1β, TNF-α, ICAM-1 and MMP-9 mRNA expressions and IL-1β and TNF-α contents. In conclusion, Shenxiong injection shows the significant protective effect on ischemia-reperfusion injury in rats. Its mechanism may be related to the inhibition of inflammatory factor expression.
Animals
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Drugs, Chinese Herbal
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administration & dosage
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Humans
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Intercellular Adhesion Molecule-1
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genetics
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immunology
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Interferon-alpha
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genetics
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immunology
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Interleukin-1beta
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genetics
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immunology
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Male
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Matrix Metalloproteinase 9
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genetics
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immunology
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Rats
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Rats, Sprague-Dawley
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Reperfusion Injury
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drug therapy
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genetics
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immunology