Objective To observe the therapeutic effect of cervical intraepithelial neoplasia Ⅱ( CINⅡ) associated high risk HPV with LEEP combined interferon and recombinant human interleukin -2.Methods One hundred and sixty patients with CIN Ⅱwere selected during Feb.2012 and Feb.2013.The patients were random-ly divided into four groups(40 patients in each group):the control group(LEEP),group A(LEEP united with in-terferon),group B(LEEP united with recombinant human interleukin -2)and group C(LEEP united with inter-feron and recombinant human interleukin -2).TCT,colposcope examination and HPV were reviewed ,and the curative effects , postoperative complications and negative conversion rate of HPV -DNA were compared after three and six months of the treatment .Results The cure rate and negative conversion rate of HPV -DNA in group C were 92.5%and 95%,which were significantly higher than others groups ( P<0.05) after six months of treatment .The time of wound healing ,duration of vaginal discharge and bleeding time in group C were also shorter than others groups(P<0.05).Conclusion The therapeutic effect of LEEP combined with interferon and recom-binant human interleukin -2 is superior to LEEP and LEEP united interferon or interleukin -2,which is not only shorter healing time ,but also fewer complications .The therapeutic method is worthy to be applied widely .

Objective To analyze the mammographic features of invasive lobular carcinoma (ILC)compared to those of invasive ductal carcinoma (IDC).Methods Twenty cases with ILC and 95 cases with IDC were retrospectively evaluated by two breast radi-ologists according to the Breast Imaging Reporting and Data System (BI-RADS)lexicon.The mammographic findings were com-pared between ILC and IDC with the independent samples chi-square test or Fisher’s exact test,as appropriate.Results Mass was the most common finding in both ILCs and IDCs,but less frequent in ILCs (36.4% vs 63.9%,P =0.008).Mass in ILCs was more frequently lobular or irregular shape with spiculated margin.Architectural distortion and focal asymmetry were more frequent in ILCs than in IDCs,however,the differences were no significant.The frequency of calcifications was not significantly different between the two groups.The distributions of calcifications were more regional in ILCs,and more segmental or linear in IDCs (P =0.01).Conclu-sion ILCs are more usually shown as non-mass appearances on mammography.ILCs that appeared as mass are more frequently ir-regular in shape with spiculated margins.

Quality management covers every corner of hospital services,which upholds full-process quality management,continuous quality improvement and smooth management with patients at the center.By means of building a 3-tier quality management framework at the hospital,development of quality management criteria,appraisal indicators and documentation and use of quality management tools,quality management defects were identified from a third-party perspective.This effort has significantly improved quality of care at the hospital,and elevated the management execution and general service quality of the hospital.

OBJECTIVE To explore the effect of connexin (Cx) 40-formed gap junctional intercellular communication (GJIC) on Photofrin- photodynamic therapy (PDT) phototoxicity in Cx40- transfected HeLa cells and its potential mechanisms. METHODS HeLa cell line stably transfected to express Cx40 was seeded at high and low cell density, respectively, to assess in vitro photosensitivity using CCK8 assay. Western blot assay was performed to detect the expression of Cx40. The intracellular ROS and Ca2 +concentrations were determined using flow cytometer. 4-HNE and ceramide were measured using ELISA assay. RESULTS Cx40-composed GJ formation at high density enhances the phototoxicity of Photofrin-PDT. When the Cx40 is not expressed or Cx40 channels are blocked, the phototoxicity in high-density cultures substantially reduces, indicating that the enhanced PDT phototoxicity at high density is mediated by Cx40-composed GJIC. The GJIC-mediated increase in PDT phototoxicity was associated with ROS and calcium-mediated stress signaling pathways. CONCLUSION The work uniquely presents the ability of Cx40-composed GJIC to enhance the sensitivity of malignant cells to PDT, and indicates that mainte?nance or increase of Cx40-formed GJIC may be a profitable strategy towards the enhancement of PDT therapeutic efficiency.

Purpose:To improve the accurate diagnosis rate for renal angiomyolipoma (RAL) before operation.Methods:Data ofthe imaging and pathological characteristics of 54 cases of patients with renal angiomyolipoma that proved by surgery pathology were analyzed retrospectively.Results:Intravenos pyelography (IVP) did not depict any specific imaging for RAL. Selective renal arteriography (SRA), Ultrasonography(US) and computerized tomography (CT) depicted specific characteristics for RAL. But it was difficult to differ malignant changes from benign ones before surgery.Conclusions:It is widely accepted that the Ultrasonography, Computerized tomography is the best method of imaging examination. When necessary it is helpful to use selective renal arteriography.

Yunnan sudden death syndrome (YSDS) is an abruptly fatal disease of unknown etiology, found mostly in central or northwestern mountain area (with altitude between 1,815 and 2,225 meters) of Yunnan province from June to September every year. It occurs mostly in young female adults, with high incidences in Lisu, Yi and Miao ethnics and high familial aggregation. The clinical manifestation of YSDS is changeful and the pathological characteristic is lack of specificity. The pathogenesis may be attributed to several factors including poor hygiene and lower socioeconomic conditions, lack of Selenium or Chromium, infection of Coxsackie B virus, mushroom consumption and special geological conditions. This article reviews the epidemiologic features, clinical manifestations, pathological features, etiology and hypothesis in order to provide clues for the research of YSDS.
Adult ; China ; Death, Sudden/pathology* ; Female ; Humans ; Syndrome

Objective To evaluate the clinical effect of edaravone injection on patients with early cerebral ischemia (ECI) after clipping of intracranial aneurysm.Methods Seventy-eight patients with intracranial aneurysms were randomly divided into control group and treatment group,with 39 cases in each group.Patients in two groups were given the treatments of high blood volume,high perfusion pressure,blood dilution and others.On the basis of control group,treatment group was intravenously dripped edaravone 30 g mixed in 0.9％ NaCl 100 mL,finished within 30 min.Control group was intravenously dripped 0.9％ NaCl 100 mL.The treatment was lasted for 14 d.The neurological function recovery and prognosis of two groups were compared.Results After treatment,the neurologic function defect (NFD) scores in treatment and control groups were (7.36 ± 5.42),(10.71 ± 4.39) points (P ＜ 0.05).Barthel scores in treatment and control groups were (49.20 ± 13.32),(37.58 ± 11.46) points (P ＜0.05).The blood flow velocity in brain and incidence of cerebral vasospasm (CVS) in treatment group were (95.38 ± 19.60)cm · s-1,12.82％ (5/39 cases).In control group,blood flow velocity in brain and incidence of CVS were (118.25 ± 17.94)cm · s-1,33.33％ (13/39 cases).There was significant difference between the two groups (P ＜ 0.05).The Glasgow outcome score (GOS) of 5 points in treatment group was 79.49％ (31/39 cases),had significant difference with that in control group,which was 58.97％ (23/39 cases,P ＜ 0.05).Serum super oxide dismutase (SOD),nitric oxide (NO) and malonaldehyde (MDA) in treatment group were (93.26 ± 13.71) U · mL-1,(5.29 ±0.53) nmol · mL-1 and (63.29 ± 10.58) mol · L-1 after treatment.Cerebrospinal fluid SOD,NO and MDA in treatment group were (61.34 ±4.26) U · mL-1,(4.40 ± 0.25) nmol · mL-1 and (1.83 ± 0.37) mol · L-1 after treatment.Serum SOD,NO and MDA in control group were (80.20 ± 11.59) U · mL-1,(6.74 ±0.32) nmol · mL-1 and (50.91 ±9.37) mol · L-1 after treatment.Cerebrospinal fluid SOD,NO and MDA in control group were (54.29 ± 6.64) U · mL-1,(5.12 ± 0.83) nmol · mL-1 and (1.50 ± 0.24) mol · L-1 after treatment.There was significant difference between the two groups (P ＜ 0.05).There were no obvious adverse drug reactions in two groups.Conclusion The scavenging effect of edaravone on oxygen free radical in serum and cerebrospinal fluid could significantly reduce cerebral artery blood flow and CVS incidence in patients with ECI after clipping of intracranial aneurysm.

AIM: To observe the dynamic alteration of myristoylated alanine-rich C kinase substrate(MARCKS) mRNA expression in rat hippocampus with acute multi-cerebral infarction,and discuss the relationship between the alteration of hippocampus MARCKS gene and ischemia damage.METHODS: The acute multi-cerebral infarction model was established by method of Kaneko.Neurological function deficits were evaluated in the behavior test.The consequences of cerebral ischemic damage were examined by histopathological analyses.The MARCKS mRNA expression was measured by semi-quantitative PCR.RESULTS: The rats in acute multi-cerebral infarction group showed different level changes of neurological function deficits.The hippocampus damage of histopathology became significant 24h after ischemia.At the same time,the MARCKS mRNA expression was upregulated at the area of rats hippocampus during ischemia,and its overexpression started 1h after ischemia,and reached maximum7d after ischemia.CONCLUSION: MARCKS mRNA of rat hippocampus overexpresses during acute cerebral ischemia.This MARCKS mRNA overexpression is related with hippocampus ischemia damage.

Objective To explore the activated brain region of acute epilepsy in cat model induced by pentylenetetrazol(FFZ)with manganese enhanced-functional MR imaging(ME-fMRI),and evaluate the application of ME-fMRI on localization of the activated brain.Methods Forty cats were divided into 4 groups by random number table method as epileptic A and B groups as well as control A and B groups. Cats of epileptic groups were injected with PTZ(55 mg/kg)intramuscularly,and those of control groups were injected with the saline at same dose.The behavior change in the epileptic and control group A was observed and electroencephalogram(EEG)was also undertaken.Cats of epileptic and control group B were performed ME-fMRI,and the percentage of the enhanced signal intensity was then calculated.Results After injection with PTZ(55 mg/kg)intramuscularly,epileptic seizure was all evoked,and then EEG recording showed spike-wave and polyspike-wave complexes.The neocortex of cats of epileptic group B was diffusely phanero-enhanced on ME-fMRI.The percent enhancement of signal intensity in cortex of frontal lobe,parietal lobe and occipital lobe was(34.6?5.7)% and that in cortex of temporal lobe with(22.9? 6.5)%,whereas those of control group B with(14.9?4.5)% and(11.6?3.2)% respectively.And there was significant difference between the above different localization of the brain in the two groups (t=-10.43,-5.46 respectively,P

Biopsy, Fine-Needle ; Carcinoma ; diagnosis ; pathology ; surgery ; Carcinoma, Medullary ; diagnosis ; pathology ; surgery ; Carcinoma, Papillary ; diagnosis ; pathology ; surgery ; Carcinoma, Papillary, Follicular ; diagnosis ; pathology ; surgery ; Diagnosis, Differential ; Goiter, Nodular ; diagnosis ; pathology ; therapy ; Hashimoto Disease ; diagnosis ; pathology ; therapy ; Humans ; Lymphoma ; diagnosis ; pathology ; surgery ; Thyroid Nodule ; pathology ; surgery ; Thyroidectomy