Objective: To assay minimal biofilm eradication concentration (MBEC) of various extraction compounds from Galla Chinensis to 5 oral bacterial biofilms, and then to set up traditional Chinese medicine empirical study methods of pharmacodynamic susceptibility. Methods:5 cariogenic bacteria strains were selected (Streptococcus mutans ATCC25175, Streptococcus sanguis ATCC10556, Lactobacillus rhamnosus AC413, Actinomyces naeslundii WVU627, Actinomyces viscosus ATCC19246) in present study. Various extraction compounds of Galla Chinensis were GCE (aqueous extract), GCE-B(300 ml/L alcohol extract), GCE-C(500 ml/L acetone extract),GCE-D(1 000 ml/L acetone extract), gallic acid and methyl gallate. ①Observed oral bacterial biofilm formation in MBECTM-Device at different-time-stages. ②MBECTM -HTP-Assay:The minimal inhibitory concentration (MIC) and minimal biofilm eradication concentration (MBEC) of various extraction compounds of Galla Chinensis on 5 oral bacteria strains were determined. Results:5 oral bacterial biofilm were readily formed on the lid of MBECTM-Device under selected condition (observed by SEM). 5 oral cariogenic bacteria growing as planktonic populations were sensitive to 6 extraction compounds of Galla Chinensis. When compared to 6 extraction compounds of Galla Chinensis, 5 oral cariogenic bacterial biofilm were 2-16 times less susceptible than growing planktonic bacteria. GCE and GCE-B are the most effective medicine against 5 oral cariogenic bacterial biofilm. GCE-C and GCE-D were effective in killing oral-bacterial biofilm at relatively high concentration, but gallic acid and methyl gallate were less effective.Conclusion:GCE and GCE-B are the most effective medicine against 5 oral cariogenic bacterial biofilm of 6 extraction compounds from Galla Chinensis. MBEC can provide a relatively accurate medicine concentration for clinical test.

Electrocardiography ; methods ; Heart Conduction System ; physiopathology ; Heart Diseases ; diagnosis ; Humans

Object To optimize the apheresis procedure of mononuclear cells from bone marrow for different clinical treatments. Methods Bone marrow mononuclear cells were separated from bone marrow using COBE Spectra apheresis system. Cell morphology, cell counts, positive expression of CD34, CD133 and CD271 were detected, and the obtaining percent of total nuclear cells and mononuclear cells were calculated. The relationships between cell-morphology and CD34, CD133 and CD271, were also analyzed. Results 1)The positive expression of CD271 was correlated with monocytes before and after apheresis (P

0.05). On the day4, day5, the expression of E-selectin protein were greatly higher than that of normal endometrium (57.52 + 7.03, 62.91 + 7.31; P

Objective:To study effects of E selectin and ligand on adhesion and implantation of embryo.Methods:The mouse hatched blastocysts were cultured in vitro and the effects of E selectin and anti sLe x on the attachment of embryos were observed. The implantation rates of embryos were observed after anti sLe x at differential concentrations was injected to uterine cavity of mouse from pregnant day 2 to day 4.Results:The attachment rate of embryos (88 0%、87 2%) at concentrations of 25 ng/ml and 50 ng/ml E selectin was significantly higher than control group (74 0%) ( P

OBJECTIVE To investigate the existence of genes for beta-lactam antibiotic resistance and for aminoglycosides modification enzymes(AMEs) in Pseudomonas aeruginosa(PAE) isolates from ICU patients and analyze the homology among strains.METHODS ?-Lactamase genes including TEM,SHV,OXA-10,PER,VEB,GES,CARB,IMP,VIM,SPM,GIM,DHA,FOX,MOX and oprD2,were detected by PCR amplication in 21 PAE isolates.The genes for AMES including aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰwere determined by PCR amplification as well.RESULTS Among 21 isolates 21(100%),2(9.5%),1(4.8%),2(9.5%)and 4(19.0%) were positive for TEM,SHV,GES,CARB and VIM genes,respectively.The deletion of oprD2 gene was found in 14 out of 21 strains.Other ?-lactamase genes were absent in all isolates.As for AME genes,aac(3)-Ⅱ,aac(6″)-Ⅰ,aac(6)-Ⅱ,ant(3″)-Ⅰ,ant(2″)-Ⅰ and aac(3)-Ⅰgenes were present in 19.0%,23.8%,9.5%,4.8%,and 19.0% of 21 isolates,However,aac(3)-Ⅰ gene was no position in any isolates.CONCLUSIONS P.aeruginosa carries various beta-lactamase and AME genes in ICU patients.Genetic cluster analysis suggested that clonal propagation result in nosocomial infection of PAE.

Objective To explore the scanning techniques of piriformis and different ultrasonographic characteristics of normal and abnormal piriformis.Methods A total of 40 cases diagnosed with unilateral piriformis syndrome underwent ultrasonic examination.Then ultrasonic scanning techniques of piriformis were summarized.Contours,thickness and smoothness of epimysium and ultrasonic echo of internal muscle texture of piriformis were compared between the normal and abnormal piriformis.The study was approved by the Third Affiliated Hospital Ethics Committee of Zhejiang Chinese Medical University (Approval no.ZSLL-JS-2016-18).Results Interruption of ultrasonic echo of ilium could be considered as ultrasonographic signs for locating piriformis quickly and accurately.Abnormal piriformis in suffering side of patients with piriformis syndrome showed obscure contours and being thicker than the other side [x2 =9.899,P =0.002;(25.81 ± 0.30)mm vs (22.29 ± 0.27)mm,t =13.604,P =0.000].Moreover,there were significant differences in comparing smoothness of epimysium and ultrasonic echo of internal muscle texture of piriformis between the two sides(x2 =23.226,P =0.000;x2 =54.848,P =0.000).Conclusions Interruption of ultrasonic echo of ilium may be an important sign for locating piriformis.Ultrasound can display piriformis clearly and distinguish ultrasonographic images of normal piriformis accurately from abnormal piriformis,which can be taken as an basis imaging for clinical diagnosis of piriformis syndrome.

ObjectiveTo observe the effectiveness of Responsar against Anopheles sinensis and Anopheles anthropophagus. MethodsThe cotton and nylon gauze absorbing Responsar solutions of 10,15 and 20 mg/m~2 (effective dosage) were used in the test. An. sinensis and An. anthropophagus raised in the lab were used as probational worms, having the knockdown power, lethiferous power and effective keeping observation. ResultsWith Responsar of 10,15 and 20 (mg/m~2) impregnating two different kinds of bednets, the KT_(50(s)) of An. sinensis and An. anthropophagus were between 3.98 and 7.30 min after touching the bednets. The knocked down mosquitoes were resumed breeding for 24 h, and the mortality was 100%. Touching nets 3 min, resuming breeding for 24 h, the mortality of mosquitoes was more than 90%. The nets hung for 180 d after impregnated with the insecticide and the knockdown power was still between 8.57 and 16.31 min for Anopheles vector, and resuming breeding for 24 h, the mortality was still 100%. ConclusionThere is strong deadly effect of Responsar to Anopheles sinensis and Anopheles anthropophagus, and the effect can keep more than 180 d.

ObjectiveTo investigate the endoscopic and clinical characteristics of gastrointestinal Behcet's disease (BD). MethodsWe analyzed the clinical and follow-up data of 27 patients with gastrointestinal BD retrospectively.Results Most common locations involved were esophagus and ileocecum,74. 1％ (20/27) and 59. 3％ (16/27), respectively. The most common parenteral symptoms were oral ulcers (85.2％), genital ulcers (25.9％), ophthalmitis (7. 4％ ) and skin damage (7. 4％ ). The most common gastrointestinal symptom was chest pain (48. 1％, 13/27). Ulcer was the basic endoscopic feature,most of which were with uneven bottom and peripheral inflammatory response. There was no difference in endoscopic features between typical BD and atypical BD. Pathology showed typical small vasculitis (44. 4％,12/27). ConclusionClinical manifestations of intestinal BD vary. Symptoms are not necessarily consistent with the involved locations. A small number of patients with BD present with gastrointestinal symptoms as the first and only symptom, with serious complications in some patients. Physicians need to be fully aware of the disease.

Objective To explore the characteristics of etiology,clinical,electroencephalogram(EEG) and prognosis of infantile spasms(IS) with focal seizures(FS).Methods The significance of age onset,seizure patterns and atteration,etiology,video-EEG(VEEG) and evolution of FS correlating to epileptic spasms(ES),which occurred in 12 cases with IS by means of clinical observation,cranial CT or MRI,VEEG monitory and follow up were investigated.Twelve cases were divided into group A,B,C according to the stages of FS occurring prece-ding,coinciding and following ES.Results Ten cases with IS were identified from focal cortical dysplasia,tuberous scleroses complex,temporal lobe cyst or scleroses and hypoxic-ischemic encephalopathy and others.Clinical manifestation presented FS at certain times during the course of the disease,and other characteristics of frequent attacks,asymmetric spasms or tonic spasms,some atypical seizures in the eyes and the head,motionless staring and focal motor seizures and other.The correlation of ES to FS occurred during one ictal episode as follows:FS→ES(6 cases),ES→FS→ES(1 case),ES→FS(3 cases).FS appeared more frequently,atypical,predominantly involving ocular,facial,oral movement or generalized convulsion,migrating or alternating seizures,associated with epileptic discharges of posterior parietal-occipital and parietal-temporal-occipital origins in group A and early period of group B;whereas in the late period of group B and group C,FS occurred less frequently and more stable,presenting complex partial seizures or secondarily generalized seizures originated mainly from frontal and surrounding areas in the lateralized or bilateral hemispheres.Regarding the natural evolution and development of IS,2 cases with early FS developed into IS;6 cases with IS evolved into Lennox-Gastaut syndrome or symtomatic generalized epilepsy,4 cases got into FS,1 case maintained in a peculiarly epileptic state with FS as well as ES until the operation at 4.5 years old,and the other case was not identified clearly.Conclusions IS coincided with FS is a special kind of aged-related FS associated with secondarily generalized seizures and epileptic encephalopathy.The multiple etiology,seizure patterns,ictal-interictal EEG,clinical evolution and prognosis of the disorder,indicate a complicated interaction of the immature cortico-subcortical abnormalities in the critical developmental period and thereafter,can be identified as a peculiar form of clinical epileptic syndrome.