Arsenic trioxide(As2O3)effectively triggers apoptosis in acute promyelocytic leukemia (APL) cells and induces complete remission and cure in most APL patients ,if they can refrain from early mortality .In the last few years,it has been under investigation as a potential treatment for non -APL leukemia and a variety of sol-id tumors.In the meantime,it has been found that As2O3 also inhibits proliferation of non -APL leukemia cell and several solid tumor cell such as liver ,esophageal ,and gastric cancer cell in vitro or in vivo in the more and more studies .The molecular mechanisms that As 2 O3 induces cellular apoptosis in non APL leukemia cell and solid tumor cells have been discussed ,which also includes building new targeting preparation in order to obtain much more satisfying therapeutic effect and relieve its cellular toxic and side effect and so on.Here, we give a summary which is connected with the research progress above .

The authors analyzed the negative impacts on hospital public welfare performance,caused by such organizational elements imbalances of public hospitals,as objective structure,allocation of rights and responsibilities,and budget resources.In view of these setbacks,they proposed countermeasures for timely solution of such imbalances and maxim optimal combination,for the purpose of more effective reform of such hospitals.

By discussion the experience of surgery teaching for foreign students by the methods of CBL+MCAI, the successful teaching experience are the followings: making sufficient preparations before lesson according to the features and difficulties in the foreign students' surgery teaching; Emphasis on CBL teaching methods and rational application of multimedia computer assisted instruction,strengthening the teaching disciphne of foreign students and the cultivation of the spirit of students' active learning.These measures stimulated the enthusiasm of students' active learning and improved the quality of teaching.

Accordingtothe analysis ofthe characters and difficulties ofskill ofanimal surgery,we divide the teaching process into three stages:theory classes,aseptic technique and basic operating techniques,animal practice.PBL+LBL double-track teaching methods is performed,which can make up the defect of PBL in practice and should be advocated and spread in the teaching of skill of animal surgery.

Objective To investigate Zhang CiGong who was traditional Chinese medicine doctor and educator ’s academic achievement. [Methods]To summarize and analysize Zhang CiGong’s learning experiences,academic thoughts and examples of clinical experiences. [Results]Zhang CiGong had no prejudice on academic schools, integrated various academic schools’s advantages and then turned into his’s own feature. Zhang CiGong researched and clarified the essence of traditional Chinese medicine theory, and made it flourish. He found the real cause of the disease when treatment,used the holistic approach and syndrome differentiation treatment. He gathered his own experience into the ancients experience and emphasis on practice when he gave Chinese Herbal. [Conclusions]Zhang CiGong’s learning was great and we should learn from him.

Objective:To investigate the expression of vascular endothelial growth factor (VEGF) in oral squamous cell carcinoma by serum-starvation and hypoxia. Methods:The expression of VEGF mRNA was evaluated in two oral squamous cell carcinomas cells TSCCa and GNM treated with serum-starvation and hypoxia.using semi-quantitative RT-PCR. The activities of VEGF in two cell lines were detected by enzyme linked immunosorbent assay (ELISA). Results:Contrast to TSCCa cells, GNM cells expressed significantly higher levels of mRNA of VEGF by RT-PCR detection. Serum-starvation and hypoxia could increased expression of VEGF mRNA in both two cell lines in vitro, the levels of mRNA of VEGF increased more in TSCCa cell lines than that in GNM cell lines when treated with serum-starvation and hypoxic. After 4 hours hypoxic treatment, the activities of VEGF increased significantly and approached the peak after 8 hours; the activities of VEGF in GNM cell lines was dramatically twice higher than that of the control group, while the activities of VEGF increased by 6 times in TSCCa cell lines. The activities of VEGF in TSCCa cell lines were higher than that of in GNM cell lines when treated with serum-starvation. Conclusion: Serum-starvation and hypoxia can up-regulate VEGF mRNA expression and activities in oral squamous cell lines in vitro,which may play an important role in the angiogenesis of oral squamous cell carcinoma.

Objective To explore the measures for nursing patients with acute hepatic graft versus host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation. Method The clinical data of 54 patients with aGVHD after hematopoietic stem cell transplantation during January 2010 and December 2013 were reviewed for summing up the nursing measures. Results In the study, 160 patients underwent allogeneic-hematopoietic stem cell transplantation and 53 of them contracted with acute hepatic-versus-host disease. Among them, 2 cases were at degreeⅣ, 10 at degreeⅢ, 23 at degreeⅡand 19 at degreeⅠ. Forty-seven cases(87.01%) were cured after careful treatment and care , 3 ( 5 . 56%) developed into cGVHD and 4 ( 7 . 41%) died . Conclusion Enhanced observation and postoperative nursing of liver, intestine and skin together with performance of mental care and dietary nursing are effective in achieving satisfactory outcomes of GVHD.

Objective To study the protective effects of propofol on isolated rat heart during global myocardial ischemia-reperfusion. Methods 36 Wistar rats were randomly divided into control group and propofol group. Each group was subdivided into 3 subgroups: preischemia, ischemia 30 minutes and ischemia-reperfusion 30 minutes group. The changes in c-fos protein and c-fos mRNA level in isolated Langendorff perfused rat myocardium were assessed by immunohistochemical technique and RT-PCR technique respectively. Results Compared to preischemia subgroup the expression of c-fos protein and c-fos mRNA level in ischemia 30 minutes and ischemia-reperfusion 30 minutes subgroups were higher significantly (P

Objective To investigate a novel therapeutic strategy in the treatment of lung cancer with diffuse pleural metastasis accompanied by malignant pleural effusion. Methods From December 1978 to February 2003, 55 patients of carcinoma of lung with pleural metastasis underwent the comprehensive therapy characterized by pleuropneumonectomy. A follow-up of all the patients after hospital discharge was conducted. Results Death due to complications was found in 2 out of the 55 patients, but remission and significant improvement of symptoms were found in the rest. A follow-up period ranging from 1 to 25 years revealed that 48 patients survived for 5-40 months (median survival time: 14 months), and 5 patients were still living and well. Conclusion Strict control of the surgical indications and radical removal of the malignant tumor can improve the quality of life and prolong the survival time of patients, even there is already metastasis to the pleura.

Objective To establish the co-culture system of marrow stromal cells (MSCs)and A?1-40 injured PC12 in vitro and to evaluate the effect and mechanisms of the system inhibiting apoptosis of PC12 induced by A?1-40.Methods MSCs and PC12 were cultured in vitro and identified by CD44 immunofluorescent staining;PC12 were damaged by A?1-40,and transferred by transwell followed by the classification into 5 groups. PI and Annexin-V co-fluorescent staining was performed,then PC12 apoptosis were detected by flow cytometry and EM;Supernatant was analyzed by enzyme-linked immunoadsordent assay (ELISA) to detected TGF-?,NGF,BDNF,and bFGF. Results About 96% MSCs showed CD44 positive cells. Co-culture group had the lowest rate of PC12 apoptosis(46.17%?8.28%,F=61.637,P0.05). Conclusion The co-culture system of MSCs and A?1-40 injured PC12 in vitro could inhibit apoptosis of PC12 induced by A?1-40. Thus grafted MSCs have the possibility to inhibit neuronal apoptosis by A?in the diseased brain.