Objective To develop a new specie-specific nest PCR to diagnose the invasive aspergillosis and to estimate the detection of circulating galactomannan by sandwich ELISA in immunocompromised patients. Methods (1) To develop an animal model of IA and collect the whole blood and tissues from the animal model. To extract the DNA from the whole blood and perform nest PCR of the DNA ;To perform the nest PCR of the clinical blood samples from immunocompromised patients (2)To detect the circulating galactomannan from the immunocompromised patients samples by use of sandwich ELISA. Results (1) In animal model, the positive rate of nest PCR and blood culture were 77.8% and 16.7%;The sensitivity and specificity of nest PCR in animal model with invasive aspergillosis are 77.8% and 100%;(2) The sensitivity and specificity of nest PCR in immunocompromised patient were 100% and 83.3%;The sensitivity and specificity of sandwich ELISA in immunocompromised patient were 55.6% and 88.2%. Conclusions The positive rate of the nest PCR was higher than that of the blood culture in animal model. The nest PCR method has good sensitivity and specificity in both animal model and patients. It will be a promising method in diagnosis of invasive aspergillosis. The ELISA method will be helpful in diagnosis of invasive aspergillosis.

In developing excellent courses on pediatrics,it is proved that improving teaching content and enhancing the building of the teaching staff,adopting effective theoretical and practical teaching and building a sound and guaranteed monitoring system of the teaching quality will have a better teaching effect.

Abortifacient Agents, Nonsteroidal ; pharmacology ; Animals ; Drugs, Chinese Herbal ; pharmacology ; Female ; Pinellia ; chemistry ; Plant Proteins ; pharmacology ; Pregnancy

Objective To investigate the action of inorganic arsenic in the induction of DNA strand breaks in primary cultured human skin fibroblasts. Methods Sodium arsenate and sodium arsenite were used as test inorganic arsenics. DNA strand breaks were assessed by single cell gel electrophoresis assay (SCGE). Results Arsenate at 1～10 ?mol/L dose_dependently induced DNA strand breaks in cells. Arsenate at lower concentrations induced mainly degree I DNA strand breaks, while the proportion of cells with degree Ⅱ DNA strand breaks increased to 50% when treated with 10?mol/L arsenate,but no cells with degree ⅢDNA strand breaks were observed.Cells treated with 1?mol/L arsenite showed no significant increase in DNA strand breaks. At the concentration of 10?mol/L, however, arsenite induced DNA strand breaks with different degrees, and the apoptotic type DNA strand breaks were the major type. Conclusion Sodium arsenate mainly induced general type DNA strand breaks and sodium arsenite induced apoptotic type,beside general type of DNA strand breaks in primary cultured human skin fibroblasts. This could be explained by their different reaction modes with DNA, and further studies were needed.

To look into the cited literatures in Medical Journal of Chinese People's Liberation Army, all citations, including its quantity, type, language and period, in journals from 1996 to 2000 was investigated. Results: The average quantity of citations was 4 59. The citations from periodical accounted for 95 62%. The citations in Chinese and English accounted for 99 64%.The citations from 1980 to 1999 accounted for 97 35%. The results suggested that the average quantity of citations was very little. The literatures of other languages and special citations should be used more.

To find out the situations of the authors who published articles in Medical Journal of Chinese People's Liberation Army in 1996～2000, the number of authors, types of collaborated articles, organizations where the authors work were investigated. The average number of authors per article was 4.64, the collaboration rate was 93 95%, most of the collaborated articles were writen by authors working in a same organization 69 54%, trans provincial collaborated articles accounted for 15 86%, collaborated articles in a same province and internationally collaborated articles accounted for 13 03% and 1 57% respectively. The results suggested that the average number of citations was very small, and the scope of contributions should be extended in order to offer more opportunities for younger authors and to increase international collaboration.

Objective To study the molecular identification method of Candida species and lay the foundation of molecular diagnosis for deep candidiasis. Methods PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 11 type strains of 9 pathogenic Candida spp. and 39 clinical Candida isolates, and the products were digested with three types of restriction endonucleases (MspⅠ,DdeⅠand HaeⅢ). Results Five patterns of products with different molecular weights were obtained after amplification from 9 Candida spp., and the products were digested with MspⅠ, DdeⅠand HaeⅢ, producing 8, 5 and 4 unique band patterns, respectively. Conclusion PCR-RFLP is reliable, specific and useful for the identification of common pathogenic Candida species.

Congresses as Topic ; Head ; surgery ; Neck ; surgery ; Otolaryngology ; Periodicals as Topic

Objective To evaluate the effect of 3-dimmentional conformal radiotherapy (3-DCRT) for mediastinal metastasis from esophageal carcinoma after surgery.Methods Sixty-three such patients were treated by 3-DCRT. Tumor diameter was: cm 20 patients. Positive supraclavicular lymph node was found in 9 patients. The number of tumor at the upper right, upper left and upper median mediastinum was 27, 27 and 20. All patients received 3-DCRT to the dose of 40?Gy (2?Gy/f, 5f/w), after resetting of portals, the dose was boost to 64～70?Gy by 3?Gy/f, 5f/w. Dose of the patients who had been irradiated was boosted to 50?Gy without further positioning. Results Complete response rate and partial response rate were 43% and 48% one month after the treatment was concluded,with no progression of disease. The 1-, 2- and 3-year survival rates were 75%, 43% and 15%. Conclusions Three-dimmentional conformal radiotherapy, with little side-effects, can markedly improve the local control rate and suvival rate of patients with mediastinal metastasis from esophageal carcinoma after surgery.

Objective To summarize the hot spots and cutting-edges by exploring Multidisciplinary Team (MDT) research in cancer.Methods CiteSpace is used to analyze MDT in cancer visually.Results 2 160 related literatures,10 key literatures,15 main clusters and 29 burst terms are detected.Conclusion MDT research on cancer mainly focuses on operation process,implemention and evaluation of effects.Improving efficiency,cost-effectiveness analysis and randomized controlled trial is the cutting-edge topics.This provides a new perspective for China.