To study the mechanism ofstimulating effect of 1,25 dihydroxyvitamin D 3 on induction of neovascularization during gastric carcinogenesis induced by N methyl N′ nitro N nitrosoguanidine in rats, gastric carcinogenesis in rats was induced by administration of MNNG (150mg/L) in drinking water. Four weeks after MNNG exposure, rats were fed with diet containing 1,25(OH) 2 D 3 (2.5?g/kg, 5.0?g/kg) . Animals were killed at week 16, and 32 for the study of neovascularization by cardiovascular perfusion with carbon ink and immunohistochemical staining of vascular endothelial cell growth factor (VEGF). Expression of VEGF and microvessel density in glandular stomach of rats receiving 1,25(OH) 2 D 3 ( 5.0?g/kg) in the diet dramatically increased when compared with the rats receiving MNNG alone at week 16, and the differences were more significant at week 32. Expression of VEGF was closely correlated with microvessel density. We propose that certain dose of 1,25(OH) 2 D 3 stimulated neovascularization during gastric carcinogenesis in rats induced by MNNG partly through increasing VEGF expression.

Enzyme histochemistry of neurons in the enteric nerve plexus of guinea pigs were studied with light microscope semi-quantitatively and microphotometer quantitatively. The results showed that the neurons differ greatly in A1P (alkaline phosphatase), AcP (acid phosphatase), 5'-Nase (5'-Nucleotidase), TPPase (thiamine pyrophosphatase), NsE (non specific esterase) and ChAT (choline acetyltransferase). There were disparities to a certain extent in reactions of MAO (monoamine oxidase), AP (aminopeptidase) and AChE (acetylcholinesterase) among different segments of gastrointestinal between submucous and myenteric plexus, but all neurons were positive for the enzymes stated above. The neurons in each ganglion were relatively similar in the enzyme activities. There were about 50-66% neurons in the enteric nerve plexus showing strong reaction of ChAT, which may be cholinergic neurons. There were significant differences in enzymatic activities, except NsE, between submucous plexus and myenteric plexus statistically. Submucous plexus showed stronger reactions of AcP and AP than those of myenteric plexus, while myenteric plexus showed stronger reactions of A1P, 5'-Nase, TPPase, MAO, ChAT than those of submucous plexus. The ganglia of intramural plexus in stomach were not well developed as those of intestine, especially the submucous plexus of stomach, in which there were only few scattered neurons, and they showed weaker enzyme activities than those of intestine. The enteric neurons in duodenum and proximal colon showed strongest activities for most enzymes among different segments of intestine. The above results indicate that the enteric neurons exist remarkable differences in metabolism and functional states.

The large majority of elderly patients undergoing ophthalmic surgery take antiplatelet and anticoagulant drugs on a regular basis. Antithrombotic treatments predisposes to bleeding complications that may lead to retrobulbar haemorrhage, suprachoroidal haemorrhage and ultimately, to loss of vision. However, discontinuation of antithrombotic medication in such patients may lead to thromboembolic events with serious consequences. There are no guidelines on perioperative management of ophthalmic patients who are on antiplatelet and anticoagulant drugs. We reviewed traditional and newer agents in the context of cataract, vitreoretinal, glaucoma and oculoplastic surgery. Recommendations are given for continuation, cessation and recommencement of these agents in order to minimise the risk of bleeding and thrombotic complications.

Objective To study the apoptosis-inducing effect of Oridonin on PC-3 cells line and the role of Survivin in the process.Methods After PC-3 cells were incubated with different concentrations of Oridonin,cell viability was analyzed with MTT assay.The percentage of earlier apoptosis cell was analyzed by flow cytometry.The protein expression of Survivin in PC-3 cells were detected by Western blot and fluorescent quantitative PCR.Results Oridonin effectively inhibited the proliferation of PC-3 cells in a concentration-time dependent way.After PC-3 cells were treated with Oridonin ( 2.5,5,10,20,40 μmol/L)for 48 hours,the cytotoxicity index were 9.2％,25.3％,39.3％,77.2％,92.5％ and the IC50 of PC-3 cells was 10.29 μmol/L,respectively.Flow cytometry was used to detect the effect of different concentration of Oridonin (0,10,20,40 μmol/L) for 48 hours,the apoptotic rates of PC-3 cells were 4.8％,15.4％,19.5％,27.4％ ( P ＜ 0.05).Oridonin down-regulated Survivin protein in a concentration-dependent way in PC-3 cells.Conclusions Oridonin can induce the apoptosis of PC-3 cells by a concentration-dependent manner.Oridonin can induce the apoptosis of PC-3 cells by down-regulated Survivin protein.

Adolescent ; Attention Deficit Disorder with Hyperactivity ; classification ; diagnosis ; epidemiology ; Child ; Comorbidity ; Female ; Humans ; Male ; Prevalence ; Prognosis

Objective To evaluate the efficacy and safety of topotecan(TPT) in the treatment of advanced epithelial ovarian carcinoma. Methods 84 patients with advanced epithelial ovarian carcinoma received TPT(1.25mg/m~2) as a 30-minute infusion daily for 1-5 days,21 days for a cycle.The efficacy was evaluated after 2 cycles of chemotherapy.Response was confirmed 4 weeks later.Results In 84 selected patients,72 were assessable for response and 84 for toxicity.The overall response was 22.2%,including 2 CR and 14 PR.The response rate for untreated and recurrent advanced epithelial ovarian carcinoma was 25.0% and 20.8%,respectively.The main side effects were neutropenia and leukopenia.WHO grade III-IV of them were 26.1% and 26.1%,respectively.The non-hemotological toxicity was mild.Conclusion TPT is effective and well-tolerated in the treatment of advanced epithelial ovarian carcinoma,especially in recurrent patients.

Objective To explore the sleep quality in patients with psoriasis. Methods Twelve psoriatic patients and 19 normal controls were examined by means of polysomnography (PSG). Results Light sleep increased markedly, but medium and deep sleep decreased in psoriatic group. Hypopnea index, apneahypopnea index, lowest oxygen saturation in arterial blood,

Objective To investigate the retinal toxicity and verify the safe dose of intravitreal injection of nonsteroid anti inflamatory drug, diclofenac sodium. Methods Twenty eight healthy adult white rabbits were divided at random into 7 groups and received in every right eye the intravitreal injection of a single dose of diclofenac sodium solution ranging from 0.4～1.0 mg/0.1ml respectively,the left eyes were regarded as control ones. Before injection and on the 1st, 3rd, 7th, 14th, 21st, and 28th day after injection the electroretinography on both eyes was examined. On the 28th day after injection the retinas of two rabbits of every group were examined by using light microscopy. On the 10th and 30th day after injection the retinal tissues around the optic nerve disk of two eyes from every group at random were tested by using transmission electron microscopy. Results The ratio of amplitude of b wave of electroretinography in 0.4 mg and 0 5 mg groups had no significant difference from groups before injection,the retinal tissues showed no structural changes in light and electron microscopy examination. The ratio of amplitude of b wave of photoptic electroretinography in 0.6 mg groups in the early stage after injection was markedly reduced ( P

The LVdP/dtmax, LVSP, AP and Vmax of the rat hearts in vivo were increased by Sophoridine ( 2 mg/kg, given to rats iv ) by 19.5, 13.1, 14.8 and 28.2% respectively. Such a positive inotropic effect lasted for more than 5 min and was statistically significant. Adrenaline (0.16 ?g/kg, iv ) could also increase the LVdP/dtmax and Vmax of the heart obviously and this action was not stronger than Sri after uses of drugs except at 0.5 min and vanished more quickly. There was an increment of MVO2I because of the increased arterial pressure within 3 min after use of Sri, which was much less than adrenaline. The present results show Sri strengthened the cardial force for longer time and affected the arterial pressure and MVO2I less than adrenaline in the rat hearts in vivo.

Deep venous thrombosis is a common and serious complication after orthopedics operation, with the characteristics of high incidence rate and death rate, its formation mechanism and the treatment is becoming more and more attention of scholars. Establishment of animal model of deep venous thrombosis can further explore the pathological process of thrombosis or dissolution, is an important means to research of thrombosis mechanism and evaluation of therapeutic method. This review discussed the basic principle of deep venous thrombosis, the selection of experimental animals and making method of animal models.
Animals ; Disease Models, Animal ; Venous Thrombosis ; etiology