1.The significance of PCD and mRNA transcription of p 53 gene during development of normal palate and cleft palate in C57BL/6N mouse
Xin LIN ; Yan JIN ; Shaozhong DONG
Journal of Practical Stomatology 2000;0(06):-
砄bjectives:To observe the difference of programmed cell death (PCD) and the expression of related gene p 53 in the development of normal palate and in the formation of cleft palate.Methods:The model of the development of cleft palat was estabished with retinoid acid (80 mg?kg 1 for each pregnant mouse). The palate samples were obtained at GD13 14 (at 13rd day 14 hours of prenancy),13 22 ,14 8,14 14 ,14 33 ,15 8,15 22 and 16 8 respectively.PCD was detected with TUNEL staining,while the mRNA transcription of p 53 was observed by in situ hybridization.Rssults:In early development of palate process,the positive index of PCD in the cleft palate samples was significantly higher than that in the normal ( P 0.05) between the two groups.Conclusion:The proliferation of mesenchymal cells of the early developmental palate process may be inhibited due to the abnomal PCD in the formation of cleft palate.The mechanism of PCD in this study may be a p 53 independed pathway.
2.Clinical Study on Qidan Granule for Treatment of 105 Cases of Pulmonary Interstitial Fibrosis
Changjun JIN ; Hongtao XIN ; Dianjie LIN
Journal of Traditional Chinese Medicine 1992;0(08):-
Objective:To investigate clinical therapeutic effect of Qidan Granule on pulmonary interstitial fibrosis(PIF).Methods:The treatment group(105 cases)were treated by Qidan Granule,3 times a day,and the control group(60 cases)were treated by prednisone,0.5mg/kg,for 3~6 months.The patients were followed up once every 1~3 months.Changes of symptoms,signs and high-resolution computed tomogram(HRCT)and pulmonary function were investigated after treatment.Results:After treatment of 6 months,the improvement rate of symptoms and signs in the treatment group was superior to that in the control group(P
4.Expression of HLA-G and IL-10 in patients with acute leukemia.
Jin YANG ; Shao-Lin ZHAO ; Xin-Ling YANG
Chinese Journal of Hematology 2012;33(2):135-137
Acute Disease
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Adolescent
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Adult
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Aged
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Child
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Female
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HLA-G Antigens
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metabolism
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Humans
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Interleukin-10
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metabolism
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Leukemia
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metabolism
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Male
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Middle Aged
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Young Adult
5.Exploration on the Research-oriented Teaching Reform of Microbiology
Yue-Lan YIN ; Xin-An JIAO ; Zhi-Ming PAN ; Lin SUN ; Jin-Lin HUANG ; Xiang CHEN ;
Microbiology 2008;0(12):-
This paper is discussed about course system construction of Microbiology, teaching method, in- struction means and experimental teaching mode. Teaching practice indicated that reform the pattern of Mi- crobiology educational mode can stimulate students’ interest in studying the course, cultivate their inde- pendent ability to solve questions, develop their creative thinking. It is an important way to train high-caliber talents.
6.Study on material base of Carthamus tinctorius with antioxidant effect based on selective knock-out.
Lin-Yan WANG ; Yu-Ping TANG ; Xin LIU ; Ya-Hui GE ; Shu-Jiao LI ; Er-Xin SHANG ; Jin-Ao DUAN
China Journal of Chinese Materia Medica 2014;39(7):1285-1289
OBJECTIVETo establish a method for studying efficacious materials of traditional Chinese medicines from an overall perspective.
METHODCarthamus tinctorius was taken the example. Its major components were depleted by preparing liquid chromatography. Afterwards, the samples with major components depleted were evaluated for their antioxidant effect, so as to compare and analyze the major efficacious materials of C. tinctorius with antioxidant activity and the contributions.
RESULTSeven major components were depleted from C. tinctorius samples, and six of them were identified with MS data and control comparison. After all of the samples including depleted materials are compared and evaluated for their antioxidant effect, the findings showed that hydroxysafflor yellow A, anhydrosafflor yellow B and 6-hydroxykaempferol-3, 6-di-O-glucoside-7-O-glucuronide were the major efficacious materials.
CONCLUSIONThis study explored a novel and effective method for studying efficacious materials of traditional Chinese medicines. Through this method, we could explain the direct and indirect contributions of different components to the efficacy of traditional Chinese medicines, and make the efficacious material expression of traditional Chinese medicines clearer.
Alkalies ; chemistry ; Antioxidants ; chemistry ; Carthamus tinctorius ; chemistry ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; Glucosides ; chemistry ; Mass Spectrometry
7.Blood transfusion for the treatment of poisoning.
Xin-guo ZHANG ; Jin-zhou BI ; Hai-shi WANG ; Hong-jun LI ; Lui-can LIN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(3):237-237
Adolescent
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Adult
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Blood Transfusion
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Child
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Child, Preschool
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Female
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Humans
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Male
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Middle Aged
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Pesticides
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poisoning
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Poisoning
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therapy
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Treatment Outcome
8.Application of the Glazer pelvic floor muscle evaluation protocol to patients with spinal cord injury
Fang CONG ; Jianjun LI ; Hongjun ZHOU ; Qiong WU ; Xin LIN ; Ying ZHENG ; Long JIN
Chinese Journal of Physical Medicine and Rehabilitation 2012;34(3):201-205
Objective To identify differences in the anal sphincter surface electromyography (sEMG)variables in spinal cord injury (SCI) subjects with different severities. Methods One hundred and ten SCI patients' impairments were classified as ASIA A,B,C or D using the International Standards for the Neurological Classification of Spinal Cord Injury.The evaluation was pedormed using sEMG equipment with an inserted anal sensor electrode and the Glazer pelvic floor muscle sEMG protocol.The sEMG variables were recorded and compared. Results There was no significant difference in sEMG variables between groups A and B,but the mean and maximum sEMG values of groups C and D in flick contractions ( 1 s),tonic contractions ( 10 s),and endurance contractions (60 s) were significantly higher than those of group A.Compared with group C,the mean and maximum sEMG values of group D were all significantly higher. Conclusions The sEMG data from the anal sphincter during contractions decreases significantly after SCI.Glazer's pelvic floor muscle sEMG protocol is a noninvasive and convenient real-time assessment.It is a useful complementary tool for quantitative assessment of the pelvic floor muscles of SCI patients.
9.Effect and mechanism of cell differentiation agent Ⅱ on the differentiation of HL-60 cells
Guorong JIN ; Xiumei LIN ; Yanli XU ; Weiwei LIU ; Xin ZHOU ; Yan TANG ; Huangxuan SHEN
Cancer Research and Clinic 2013;(6):361-364
Objective To investigate the effect and mechanism of cell differentiation agent Ⅱ (CDA-Ⅱ) on the differentiation of human acute mycloid leukemia (APL) HL-60 cells.Methods The cell morphology and differentiation was detected by Wright-Giemsa staining,the expression of cell surface differentiation antigen CD11b of HL-60 was detected by flow cytometry,the differentially expressed genes were screened by gene expression profile chip (NimbleGen).Results The result of Wright-Giemsa staining showed that CDA-Ⅱ induced HL-60 differentiation towards mature stages in a time-dependent manner.After treated with CDA-Ⅱ,the percentage of CD11b-positive HL-60 cells significantly increased in a time-and dose-dependent manner.The result of gene expression profiling indicated differentially expressed genes including 113 up-regulation genes and 140 down-regulation genes.The up-regulation expression genes involved in six pathways including mineral absorption,complement and coagulation cascades,down-regulation expression genes involved in nine pathways including pyrimidine metabolism,RNA polymerase,purine metabolism and so on.Conclusion CDA-Ⅱ can induce HL-60 differentiation and make gene differentially expressed.The data provide the feasibility of CDA-Ⅱ in differentiation induction therapy for APL.
10.Evaluation of Post-operative Complication in Patients With Total Anomalous Pulmonary Venous Connection by Echocardiography
Jingya LI ; Fangyun WANG ; Xiaofeng LI ; Lanzhong JIN ; Xin ZHANG ; Guiqin MA ; Lin ZHENG ; Haiyan WEI
Chinese Circulation Journal 2014;(8):598-601
Objective: To evaluate the post-operative complication of pulmonary vein stenosis in patients with total anomalous pulmonary venous connection (TAPVC) by echocardiography.
Methods: A total of 66 TAPVC pediatric patients with surgical repair in our hospital were retrospectively studied. The peri-operative and mid-term post-operative echocardiography was conducted to evaluate the complication of pulmonary vein stenosis.
Results: The peri-operative and mid-term post-operative echocardiography presented the increased left ventricular diastolic end diameter (LVEDd) and decreased right ventricular diameter in all 66 patients, P<0.05. There were 6 patients with pulmonary vein stenosis showed high velocity Doppler signals at atanastomosis. The peri-operative echocardiography in those 6 patients showed increased LVEDd, decreased right ventricular diameter and pulmonary artery pressure, all P<0.05. The mid-term post-operative echocardiography in those 6 patients indicated increased LVEDd, P<0.05, while the right ventricular diameter and pulmonary artery pressure were similar before and after the operation, P>0.05.
Conclusion: Echocardiography may monitor the operative effect of TAPVC, the occurrence of post-operative pulmonary vein stenosis could be found by examining right ventricular diameter and pulmonary artery pressure in pediatric patients.