Purpose　To explore the effects of antibiotics and herbs with removing pathogenic heat from blood on ovarian granulosa cell morphological and functions in female rats.　Methods　The granulosa cell of 30 d age SD female rats was collected,several drugs were added to the culture fluid.The culture fluid were collected to measure the sex hormone by radioimmunoassay.The cell organs of granulosa cell were measured by transmission electron microscope.　Results　Organs of the granulosa cell almost disappeared and perinuclear vesicle were founded in Amikacin sulfate group and Rhizoma zedoariae group.The progesterone levels were lower than in salline group (P<0.05).The estrogen levels of Amikacin group were lower than in saline group (P<0.05).　Conclusions　Ovarian function were obstructed in female rats when Amikacin,Rhizoma zedoariae were directly used.

Objective Detect the AChE content of RBC and plasma in the people with PNH.Invstigate the effect of the enzyme in GPI on PNH.Methods Detect the activity of AChE in RBC and plasma in 30 patients with PNH.Results (1)The activity of AChE of RBC in PNH patients was higher than controls,the difference between them having statistic significance,P

Objective To study the diagnosis and therapy of Hashimoto's disease (HD) with thyroid nodule. Methods The clinical data of 187 patients with nodulous Hashimoto's disease who had operation in our hospital from 1995 to 2008 were analysed retrospectively. Results The nodules coexisting with Hashimoto's disease were thyroid carcinoma, nodular goiter, thyroid follicular adenoma and lymphoma accounting for 11.2% (21/187), 26.7% (50/187), 15% (28/187) and 1.6% (3/187) respectively. Conclusions HD patients with thyroid nodules especially the single nodule that can not be distinguished from cancer should have operation in time. Ultrasound guided needle aspiration will be helpful for the preoperative diagnosis and the frozen section may guide the surgical modality.

This article briefly summarized conventional k-space technique, partial k-space sampling and key hole technique, then introduced the latest applications during these years.
Magnetic Resonance Imaging

Aim To explore the effect of sesquiterpene lactone parthenolide(PL) on primary multiple myeloma(MM) cell and cell lines and its molecular mechanisms.Methods Fresh bone marrow cells from MM patients and MM cell lines KMM-1 and MM1S were used for in vitro culture study.The cell survival rate was examined by Trypan Blue exclusion.The change of nucleolus stained with DAPI was observed under fluorescent microscope.Reactive oxygen species(ROS) levels and mitochondrial membrane potential(MMP) were detected by flow cytometry.Results 2.5 ?mol?L-1 PL induced MM cell apoptosis and MMP was profoundly decreased in KMM-1 and MM1S cells.In contrast,normal lymphocytes cells were unaffected by the same conditions.KMM-1 and MM1S cells showed large increases of ROS levels 10 h after 2.5 ?mol?L-1 PL treatment.The free radical scavenger L-N-acetylcystein(L-NAC) pretreatment completely inhibited ROS generation,cell death and nuclear fragmentation by PL.Diphenylene iodonium chloride(DPI) pretreatment in a dose of 2.5 ?mol?L-1 strongly inhibited PL-mediated ROS generation,nuclear fragmentation in KMM-1 and MM1S cells.Conclusions PL strongly induces apoptosis in MM cell,while normal lymphocytes cells are completely unaffected by the same conditions.Since its proapoptotic activity appears to be mediated through induction of oxidative stress in MM cells,its possible mechanism is that NADPH oxidase activation is involved in PL-mediated ROS generation.

AIM To study the effects of H 2O 2 on transient outward potassium current METHODS Whole cell patch clamp technique was used to record changes of I A in the cultured rat hippocampal neurons RESULTS After exposure to 30 ?mol?L -1 H 2O 2 for 12 h (1) decreased I A current density from (53 8?15 8) PA?PF -1 to (18 5?6 2) PA?PF -1 ( n=10,P

Purpose To investigate the influence of single slow intravenous infusion of dexmedetomidine (Dex) on femoral artery color Doppler hemodynamics during the induction of general anesthesia. Materials and Methods Forty patients of elective abdominal surgery under general anesthesia were elected and randomly divided into Dex group and the control group, with 20 cases in each group. Dex (0.2 μg/kg) 20 ml was injected with intravenous infusion in Dex group, and 20 ml saline was infused in control group, the infusion time was 10 min;then intravenous injection of Sufentanil, Propofol and Rocuronium were performed in turn for the induction of general anesthesia and endotracheal intubation. Femoral artery peak systolic velocity (Vs), early diastolic reverse peak velocity (Vd), systolic diameter (Ds), diastolic diameter (Dd), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and heart rate (HR) changes at each time point were observed and recorded. Results ① Compared with baseline, Vs increased and Vd decreased (P<0.05) 10 min after the infusion of Dex in Dex group;after induction, Vs and Vd in both of the two groups decreased, and the difference between the two groups was not statistically significant (P>0.05);Vs reduced significantly in both of the two groups at the intubation moment and 1, 3, 5 min after intubation, but the Dex group changed more smoothly (P<0.05), and Vd raised in both groups with the Dex group changing more smoothly (P>0.05);Ds and Dd of both groups did not change significantly at each time point (P>0.05). ② Compared with baseline, MAP and HR decreased (P<0.05) after the infusion of Dex in Dex group;after induction, MAP and HR of both groups decreased, and the difference between the two groups was not statistically significant (P>0.05); MAP and HR increased in both groups at intubation moment, but the Dex group changed more smoothly than the control group (P<0.05).③SBP, DBP and HR were negatively correlated with Vs (r=-0.507,-0.619,-0.750, P<0.05) in both groups; SBP, DBP and HR were positively correlated with Vd (r=0.821, 0.881, 0.883, P<0.05) in both groups;there was no significant correlation (r=0.419, P>0.05) between Vs and Vd. Conclusion Single slow intravenous infusion of Dex (0.2μg/kg) can accelerate the femoral artery Vs and slow down the Vd, resulting in more stable hemodynamics during the induction of general anesthesia.

To explore the clinical advantages of interventional radiologyin saving the patients with fatal hemorrhage. Simple embolization had been used in 19 patients. 8 patients were treated with temporary blockage of blood flow plus surgical procedures. Long term follow up in 26 cases showed good clinical effect. Interventional radiology, with its special technique and advantage,may be the most effective method for saving the patient with various fatal hemorrhage and in the development of traumatic surgery. It is not only a life saving proceduce,but also an important treatment in decreasing the rates of amputation and disability when it is closely coordinated with medical and surgical treatments.

AIM To study the effects of propafenone on Kv 4 2 and Kv4 3 potassium current. METHODS Whole cell patch clamp technique was used to record changes of Kv4 2 and Kv4 3 potassium currents. RESULTS ① Propafenone redu ced the amplitude of Kv4 2 and Kv4 3 currents, in a concentration-dependent, IC 50 was 10 3 and 71 ?mol?L -1 respectively. ② Propafenone inhibi ted Kv4 2 and Kv4 3 current inactivation, 10 ?mol?L -1 propafenone acce lerates Kv4 2 current inactivation constant from (38 9?2 1) ms to (9 9?1 8) ms; and shifted the steady state inactivation curves of Kv4 2 current to negative potentials. 100 ?mol?L -1 Propaf enone accelerates Kv4 3 current inactivation constant from (144 8?20 8) ms t o (18 5?2 8) ms; and shifted the steady state inactivation curves of Kv4 2 c urrent to negative potentials. ③ propafenone inhibited Kv4 2 and Kv4 3 curren t activation, 10 ?mol?L -1 propafenone shifted the steady state activati on curves of Kv4 2 current to negative potentials. 100 ?mol?L -1 propafe none shifted the steady state activation curves of Kv4 2 current to negative po tentials. CONCLUSION Propafenone, maybe, also exerts its effects via blocking Kv4 2 and Kv4 3 current to antiarrhythmia.

Objective To investigate the training effect and application development of the health industry standard of Nursing Grading, and to explore it'sclinical applicability. Methods An electronic questionnaire survey was conducted among nurses in 30 three general hospitals of Shandong Province from November 10 to December 10 in 2015. The questionnaire mainly included four parts: basic information, training status, application status and the clinical applicability. All the questionnaires were automatically submitted by the nurse′s cell phone or computer, 3386 valid questionnaires were recovered. Results The status of standard training:93.92%(3180/3386) nurses received a comprehensive training about the standard. The answer rates of 4 theoretical examination questions was 88.95%(3012/3386), 84.49%(2861/3386), 85.17%(2884/3386), 65.71%(2225/3386)respectively. 77.26%of the nurses needed to continue training. Application status: 97.67% (3307/3386) nurses in the hospital has begun to apply Nursing Grading standard, and 97.01% (3208/3307) and 97.76% (3231/3307) of the hospital revised the standard of care systems and work processes; 78.23% (2649/3386) nurses divide the level of care based on both the patient's condition and self-care ability presently. 74.45%(2521/3386) nurses did it as follows,the doctor determine the patient′s condition level, the nurse determine the level of patient′s self-care ability, then the nursing grading was determined according to the both. In the clinical applicability survey, the choice proportion of very agree and agreeofHelping nurses to determine the patient′s level of care and It is beneficial to improve the efficiency of nursing management was 86.30% (2922/3386) and 82.66%(2799/3386);The choice proportion of very agree and agree ofHelp nurses to improve the quality of nursing services, reflecting the value of the nursing work, to meet the needs of patients was 79.36% (2687/3386), 76.56% (2592/3386), 80.98% (2742/3386) respectively. Conclusions The hospital attaches great importance to the standard of Nursing Grading, but the training effect needs to be improved;standard has been widely used in Shandong Province Physician-nurse collaboration has become the decision-making main body of nursing grading;the standard application can not only help the nurses to do decision-making correctly and improve the efficiency. It also can help nurses to improve the quality of nursing service, and enhance patients′satisfaction. So it is very suitable for clinical practice.