Objective To study the therapeutic effect of sea buckthorn oil on allergic contact dermatitis(ACD) in mice. Methods A total of 60 ICR mice were randomly divided into 6 groups: blank control group, model control group,three groups of high-dose, medium-dose and low-dose sea buckthorn oil(100%, 50%, 25%, ad us. ext.), respectively,and positive control group(compound dexamethasone acetate cream), with 10 mice in each group. The mouse model of ACD was established by sensitization and stimulation with 2,4-dinitrochlorobenzene(DNCB),and injuries in the ear skin of the mice were scored and used as the indicator of successful establishment of the model. The degree of ear swelling and the spleen index of the mice in each group were measured at 48 h after stimulation. Hematoxylin and eosin staining was used to observe the pathological changes in the mouse ear skin. Results Measurement of the degrees of ear swelling showed that the high-dose sea buckthorn oil group(9 mL/kg,ad us. ext.)obviously inhibited the DNCB-induced ear swelling in the mice, with a significant difference compared with the model group(P < 0.01)and non-significant difference compared with the positive control group(P> 0.05). The result of the spleen index assessment showed that sea buckthorn oil at each dose may play an immune-regulatory role by promoting the development of immune organs. The pathological examination showed that epidermis thickening of the ear tissue,epidermal and dermal edema and infiltration of lymphocytes were reduced, dermal vasodilatation and congestion were alleviated, and inflammatory cell infiltration was inhibited in the three groups of different doses of sea buckthorn oil compared with the model control group. Conclusions Sea buckthorn oil has an obvious inhibitory effect on the DNCB-induced allergic contact dermatitis in mice. However, its related mechanisms remain to be further investigated.

OBJECTIVETo investigate the anti-aging effect of aqueous extract of Hedysarum austrosibiricum cultivated in Xin-jiang.

METHODSubacute aging model in mice was established by D-galactose (D-gal) and activities of SOD and GSH-PX, contents of MDA in brain and liver tissues, activities of MAO in brain tissue and immune indexes were determined.

RESULTAqueous extract of H. austrosibiricum Xinjiang markedly increased the activities of SOD and GSH-PX, significantly decreased contents of MDA in brain and liver tissues. MAO activities in brain tissue were also decreased. It also elevated the spleen and thymus indexes.

CONCLUSIONAqueous extract of H. austrosibiricum might have anti-aging effect, which is implemented by eliminating oxyen free radicals, rising activities

Aging ; drug effects ; metabolism ; Animals ; Antioxidants ; isolation & purification ; pharmacology ; Brain ; drug effects ; metabolism ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Fabaceae ; chemistry ; Female ; Free Radical Scavengers ; isolation & purification ; pharmacology ; Free Radicals ; metabolism ; Galactose ; toxicity ; Glutathione Peroxidase ; metabolism ; Liver ; drug effects ; metabolism ; Malondialdehyde ; metabolism ; Mice ; Monoamine Oxidase ; metabolism ; Plant Extracts ; isolation & purification ; pharmacology ; Plant Roots ; chemistry ; Random Allocation ; Superoxide Dismutase ; metabolism

Adult ; Cholesterol ; Dilatation ; Ear Canal ; Ear Neoplasms ; surgery ; Ear, Middle ; pathology ; Eustachian Tube ; Female ; Granuloma ; surgery ; Humans ; Male ; Mastoidectomy ; Middle Aged ; Treatment Outcome

This paper proposes an algorithm of evaluating the compression depth, and then to extract four normalized mammary elasticity characteristic parameters with respect to the compression depth. The classification experiments show that these elasticity parameters have a good capability in determining whether the tumor is benign or malignant, and if combined with morphological parameters, the accuracy, sensitivity and specificity can be improved and increased to 95.19%, 98.82% and 92.16%, respectively.
Algorithms ; Breast Neoplasms ; diagnostic imaging ; Elasticity Imaging Techniques ; Female ; Humans ; Sensitivity and Specificity ; Ultrasonography, Mammary ; methods

ObjectiveTo explore the preventive effects of chest compression， abdominal compression and combined thoracoabdominal compression on propofol-induced respiratory depression during gastroscopy. MethodsA total of 544 patients underwent propofol sedation during gastroscopy in our hospital were randomly divided into 4 groups （n=136 each）： Group C （control group）， Group T （chest compression group）， Group A （abdominal compression group） and T&A （combined thoracoabdominal compression group）. Altogether 20 chest， abdominal and combined thoracoabdominal compressions were started respectively on patients in Group T， A and T&A before performing gastroscopy and after unconsciousness at a rate of 30 compressions per minute with a compression depth of 2~3 cm. The incidence of oxygen desaturation （SpO₂2<95%） and hypoxemia （SpO₂<90%） were compared among the groups. Incidence of gastroscope withdrawal and B-mode ultrasound assessment of diaphragm activity were observed. ResultsThere were statistical differences in the incidence of oxygen desaturation and hypoxemia among the four groups （P<0.05）. The incidence of oxygen desaturation in Group T and A were significantly lower than that in Group C （P<0.017）. B-ultrasonography revealed that the diaphragm activity in Group T and T&A were significantly higher than that in Group C （P＜0.01） and the patients in Group T had the highest diaphragm activity. ConclusionBoth chest compression and abdominal compression can reduce the incidence of respiratory depression induced by propofol in patients during gastroscopy， and chest compression is more effective.

OBJECTIVE: To noninvasively assess the neurodegenerative changes in the brain of patients with Niemann-Pick type C (NPC) disease by measuring the lesion tissue with the iterative decomposition of water and fat with echo asymmetry and least square estimation-iron quantification (IDEAL-IQ). MATERIALS AND METHODS: Routine brain MRI, IDEAL-IQ and 1H-proton magnetic resonance spectroscopy (1H-MRS, served as control) were performed on 12 patients with type C Niemann-Pick disease (4 males and 8 females; age range, 15–61 years; mean age, 36 years) and 20 healthy subjects (10 males and 10 females; age range, 20–65 years; mean age, 38 years). The regions with lesion and the normal appearing regions (NARs) of patients were measured and analyzed based on the fat/water signal intensity on IDEAL-IQ and the lipid peak on 1H-MRS. RESULTS: Niemann-Pick type C patients showed a higher fat/water signal intensity ratio with IDEAL-IQ on T2 hyperintensity lesions and NARs (3.7–4.9%, p < 0.05 and 1.8–3.0%, p < 0.05, respectively), as compared to healthy controls (HCs) (1.2–2.3%). After treatment, the fat/water signal intensity ratio decreased (2.2–3.4%), but remained higher than in the HCs (p < 0.05). The results of the 1H-MRS measurements showed increased lipid peaks in the same lesion regions, and the micro-lipid storage disorder of NARs in NPC patients was detectable by IDEAL-IQ instead of 1H-MRS. CONCLUSION: The findings of this study suggested that IDEAL-IQ may be useful as a noninvasive and objective method in the evaluation of patients with NPC; additionally, IDEAL-IQ can be used to quantitatively measure the brain parenchymal adipose content and monitor patient follow-up after treatment of NPC.
Brain ; Female ; Follow-Up Studies ; Healthy Volunteers ; Humans ; Magnetic Resonance Imaging ; Magnetic Resonance Spectroscopy ; Male ; Methods ; Niemann-Pick Diseases ; Proton Magnetic Resonance Spectroscopy ; Water

OBJECTIVETo compare oncologic adequacy of resection and long-term oncologic outcomes between laparoscopic-assisted surgery (LS) and open surgery (OS) in the treatment of rectal cancer.

METHODSLiterature searches of electronic databases (PubMed, Embase, Web of Science,and Cochrane Library) and manual searches up to June 30, 2010 were performed to identify RCTs comparing values of oncologic adequacy of resection, recurrence and survival following LS and OS. Fixed and random effects models were used.

RESULTSSix RCTs enrolling 1033 participants (LS group:577 cases, OS group:456 cases)were included in the meta-analysis. Number of lymph node harvested was similar(WMD=-0.38, 95%CI:-1.35-0.58, P=0.43). LS had a slightly higher circumference resection margin(CRM) positive rate with no statistical significance[7.94% vs. 5.37%; risk ratio(RR)=1.13; 95%CI:0.69-1.85, P=0.63]. There was no significant difference between the two groups in local recurrence (RR=0.55; 95%CI:0.22-1.40, P=0.21). The 3-year overall survival [Hazard ratio(HR)=0.76; 95%CI:0.54-1.07, P=0.11] and 3-year disease-free survival(HR=1.16; 95%CI:0.61-2.20, P=0.64) were not significantly different between the two groups.

CONCLUSIONCompared with open surgery, laparoscopic surgery of rectal carcinoma offers similar oncological clearance and long-term oncological outcomes.

Humans ; Laparoscopy ; Laparotomy ; Randomized Controlled Trials as Topic ; Rectal Neoplasms ; surgery ; Treatment Outcome

Adolescent ; Cardiac Catheterization ; methods ; Child ; Child, Preschool ; Echocardiography ; Female ; Heart Septal Defects, Atrial ; diagnostic imaging ; surgery ; Humans ; Infant ; Male

Objective To investigate the effect of Citronella oil on dementia of Alzheimer's type induced by D-galactose and aluminum trichloride (AlCl3) and explore its mechanism.Methods The 60 healthy KM mice with sex in half were enrolled in this study,10 of them were taken randomly as the normal group,the remainder 50 mice were in the model group.The rats received inntraperitoneal injection of D-galactose and oral aluminum trichloride for consecutive 10 weeks to construct the disease models.The model rats were randomly divided into five groups:model group,control group (0.5 g · kg-1 Piracetan) and three doses (0.4,0.6,0.8 mL · kg-1 Citronella oil) experimental groups.The ability of learning and memory was measured by the Morris water maze test.The levels of glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) in serum homogenate and acetylcholine (ACh) in brain tissue homogenate were determined by spectrophotometer.Results At the fifth day in the Morris water maze training period,the escape latency(EL;s) in the normal group,model group,control group and three doses experimental groups were 21.42 ±-3.18,43.33 ± 2.14,26.84 ± 0.71,23.16 ± 3.70,18.48 ± 1.11,21.40 ±0.88.Compared with the normal group,the EL in model group was increased significantly (P ＜ 0.05).Compared with the model group,EL in control group and three doses experimental groups reduced significantly (P ＜ 0.05).The levels of GSH-PX and MDA in serum in the normal group,model group,control group and three doses experimental groups were (299.05 ± 90.62),(246.19 ± 37.90),(532.49 ± 121.52),(587.21 ± 177.37),(497.14 ±82.95),(493.33 ±73.64)U · mL-1;(9.38 ±3.01),(14.89 ±4.41),(6.98 ±2.43),(6.06 ±3.34),(5.26 ± 1.90),(5.01 ± 1.59) mmol · mL-1.Compared with the normal group,the GSH-PX and MDA in model group was decreased or increased significantly (all P ＜ 0.05).Compared with the model group,the GSH-PX and MDA in control group and three doses experimental groups reduced significantly (P ＜ 0.05,P ＜ 0.01).The level of ACh in brain tissue in the normal group,model group,control group and three doses experimental groups were (268.27 ±25.40),(224.95 ±21.52),(392.56 ±50.58),(240.17 ± 17.15),(255.50 ± 15.91),(259.15 ±20.74) μg · mL-1.Compared with the normal group,the ACh in model group was decreased significantly (P ＜ 0.01).Compared with the model group,the ACh in control group and middle and high doses experimental groups increased significantly (P ＜ 0.05,P ＜ 0.01).Conclusion Citronella oil,like Piracetam,enhanced the antioxidant activity of the Alzheimer's type induced by D-galactose and AlCl3,and increased the synthesis of ACh in brain.

OBJECTIVETo search for differentially expressed proteins in the serum of patients with Crohn's disease.

METHODSSerum protein samples obtained from 4 patients with Crohn's disease and 4 normal adults were cross-labeled with different CyDyes and underwent two-dimensional differential in-gel electrophoresis (2-D DIGE) and imaging analysis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to identify the differentially expressed proteins.

RESULTS2-D DIGE revealed that the protein on spot 973 was overexpressed by 2.55 folds in the serum of patients with Crohn's disease compared with that in normal adults (P<0.05). The protein was identified as CD45 using mass spectrometry.

CONCLUSIONCD45 overexpression in the serum of patients with Crohn's disease may play a role in the disequilibrium of the immune system.

Amino Acid Sequence ; Case-Control Studies ; Crohn Disease ; blood ; immunology ; Electrophoresis, Gel, Two-Dimensional ; Female ; Humans ; Leukocyte Common Antigens ; blood ; Male ; Molecular Sequence Data ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization