Objective To investigate the level of serum homocysteine(Hcy)in common chronic diseases(diabetes,hypertension, hyperlipidemia,coronary heart disease)and its clinical application value in the community elderly residents.Methods A retrospec-tive study was conducted on the common chronic disease of 1 605 patients who detected Hcy in our center from January 2015 to Oc-tober 2015.Another 117 healthy subjects served as normal control group.Hcy was detected by cyclic enzymatic method.Results The average level of Hcy in serum of patients with diabetes,hypertension,coronary heart disease and hyperlipidemia was higher than that in the health examination group,and the level of Hcy was positively correlated with age(P <0.05).Conclusion The high level of serum Hcy is an important risk factor in common chronic diseases in the community elderly residents,and it should strengthen the examination of Hcy and active intervention to slow down the development of the disease.

Object To study the different fingerprints of hydrophilic and hydrophobic components in roots of Salvia miltiorrhiza Bge. collected from different habitats, and discovery the relationship between the two types of components. Methods The fingerprints were detected by RP-HPLC and the results were analyzed by SPSS software. Results Roots of S. miltiorrhiza collected from different habitats showed different fingerprints of hydrophilic and hydrophobic components, there is no obvious relationship between the two types of components. Conclusion In order to control the quality of 5. miltiorrhiza roots that is used to manufacture injections, we must assay the hydrophilic components, such as salvianolic acid B should be determinded.

ObjectiveTo observe the effectiveness of Responsar against Anopheles sinensis and Anopheles anthropophagus. MethodsThe cotton and nylon gauze absorbing Responsar solutions of 10,15 and 20 mg/m~2 (effective dosage) were used in the test. An. sinensis and An. anthropophagus raised in the lab were used as probational worms, having the knockdown power, lethiferous power and effective keeping observation. ResultsWith Responsar of 10,15 and 20 (mg/m~2) impregnating two different kinds of bednets, the KT_(50(s)) of An. sinensis and An. anthropophagus were between 3.98 and 7.30 min after touching the bednets. The knocked down mosquitoes were resumed breeding for 24 h, and the mortality was 100%. Touching nets 3 min, resuming breeding for 24 h, the mortality of mosquitoes was more than 90%. The nets hung for 180 d after impregnated with the insecticide and the knockdown power was still between 8.57 and 16.31 min for Anopheles vector, and resuming breeding for 24 h, the mortality was still 100%. ConclusionThere is strong deadly effect of Responsar to Anopheles sinensis and Anopheles anthropophagus, and the effect can keep more than 180 d.

Particle radiotherapy using proton and heavier-ion beam was first proposed for clinical application by Robert Wilson in 1946. Compared to conventional photon radiation, proton and heavier-ion beam has significant physical advantage, and heavier-ion has unique biological characteristics. With the development of accelerator and radiation technique, it is being investigated for tumor treatment in many clinical centers. This article reviews the current status of clinical application of particle therapy using proton and heavier-ion beam in digestive system tumor.
Animals ; Digestive System Neoplasms ; therapy ; Heavy Ion Radiotherapy ; Heavy Ions ; therapeutic use ; Humans ; Protons ; therapeutic use

Objective To primarily study the possible biological significance of yon Willebrand factor (vWF) expression at the fracture site in the mechanism of fracture healing. Methods A total of 28 male SD rats were selected to set up femoral fracture models. Then, tissue samples from fracture site were randomly taken at days 2, 3, 5, 7, 10, 14 and 21 after establishment of the models, with 4 rats at each time point, vWF expression at fracture site and the relationship of vWF expression with osteoblasts and chondroblasts were observed by using computer image analysis system. The biological significance of vWF expression at fracture site in mechanism of fracture healing were analysed based on the results of vWF expression of in vitro cultured rat osteoblasts. Results The expression of vWF at every time point was positive and distributed disproportionally irregular at bone trabecula and soft tissue of the fracture site. vWF expressed positively in the extracellular matrix around the chondroblasts. Conclusions During the course of fracture healing, there may be vWF-platelet activation pathway that can regulate differentiation and proliferation of local osteoblasts and chondrocytes and activate platelets secreting growth factors such as PDGF, IGF-1, EGF and TGF-a, as creates a sound microenvironment for fracture healing.

Objective To establish a high speed and effective real-time PCR assay to analyze apolipoprotein E(apoE)genotyping in Chinese population using hybridization probes and melting curves. Methods Lightcycler was used to analyzed two codons'polymorphism after condition was optimized. The persons elder than 60 years including 133 patients with abdominal fat and 108 healthy elder were selected. The detection probes were labeled with L-Cred 640 and LC-Red 705 at 5'end covers codons 112 and 158 with the corresponding anchor probes labeled with fluorescein at 3'ends.A 265-bp fragment of the apoE gene was amplified from human genomic DNA to produce FRET.Depending on the various types of base-pair mismatch in the heteroduplex,wild type and mutant type were differentiated.Results The peaks represented the sequence-specific melting points(Tm) and each genetype showed perfect peak.E2/3 and E3/4 in abdominal fat group were much more common allele than health persons(x2=4.210.P＜0.004,x2=6·328,P＜0.012).The frequencies of abdominal fat group was E2/3(27.8%),E3/4(24.8%),E3/3(42.1%),E2/4(2.3%),E4/4(2.2%)and E2/2(0.8%).The frequencies of healthy controls were E2/3(16.7%),E3/4(12%),E3/3(68.5%),E2/4(1.9%),E4/4(O%)and E2/2(0.9%).It showed high agreement as compared with DNA sequencing analysis The expression of apoE in abdominal fat group (101.5±73.6)was up-regulated than the healthy group(50.6±27.1,P＜0.01).Conclusions Apolipoprotein E genotyping method by melting curve is faster and simpler than other technique. It can prevent the cross-contaminated and is suitable to be applied in clinical diagnosis.There was significant difference between the two groups.There was positive relationship between the elder's abdominal fat and apoE gene polymorphism. The genotyping of E3/4.E2/3 or E4/4 had the important role in the elder's abdominal fat on genetic susceptibility.

《Classification of Chinese Library》and《MeSH》 were reconstructed by analyzing the advantages and disadvantages of《Classification of Chinese Library》in semantic tagging of library resources.Ontology of dermatology was constructed and its semantic dictionary was edited on the semantic metadata-aided platform according to the theory of semantic tagging resources with skin diseases as an example in order to achieve semantic tagging resources.

Objective To compare the susceptibility of Anopheles anthropophagus from Jiangsu, Guangdong and Liaoning provinces in China to Plasmodium vivax.Methods The blood samples of patients with P. vivax in endemic areas of China were collected to feed the mosquitoes of An. anthropophagus from different areas by using the artificial in vitro membrane feeding system in the lab, and then the mosquitoes were dissected during the 7-9th day and on the 14th day after the feeding and the oocysts and sporozoites in the stomach and salivy gland of mosquitoes were counted. Results The mosquitoes from Jiangsu, Guangdong and Liaoning were simultaneously fed with the blood of 35 cases of P. vivax. The oocyst positive rates of An. anthropophagus from Jiangsu, Guangdong and Liaoning during the 7-9th day after the feeding were 68.57%, 60.00% and 68.57%, as well as the sporozoite positive rates of them on the 14th day after the feeding were 22.86%, 14.29% and 22.86%, respectively. On the 7th day after the feeding, 228, 235, 228 mosquitoes of An. anthropophagus from Jiangsu, Guangdong and Liaoning were dissected, and the positive mosquito rates with oocyst infection were 28.07%, 25.11% and 26.75%, respectively. On the 14th day after the feeding, 150, 142, 135 mosquitoes of An. anthropophagus from the three areas were dissected, the positive rates with sporozoite infection were 10.67%, 8.45% and 11.85%, respectively. The num-bers of mosquitoes dissected with infective grade("+","++","+++","++++") of sporozoites of An. anthropophagus from Jiangsu, Guangdong and Liaoning were 4, 3, 2, 7; 2, 2, 3, 7 and 1, 6, 3, 8, respectively. Conclusions An. anthropophagus from Jiangsu, Guangdong and Liaoning is susceptible to the parasites of Plasmodium vivax and there is no significant difference among the susceptibilities of An. anthropophagus from the three areas to Plasmodium vivax.

Objective To analyze the genetic characteristics of deltamethrin resistant strain of Anopheles anthropophagus. Methods The study was performed with LC-P curve method for the genetic mode of deltamethrin resistant of An. anthropophagus. Results The resistant coefficient of F_1 generation from cross and reciprocal cross were 15.19 and 16. 66 respectively. The LC-P curve of F_1 generation got near to that of resistant parents (D=0.651). There were no obvious platforms in 50% mortality of BC generation and 25% - 75% mortality of F_2 generation.There were significant differences between the determining curve and theoretical cure in both BC and F_2 generations, P

Objective To understand the resistance of Anopheles vectors to pyrethroid after several years mosquito control with the impregnated bed net method. Methods The resistant level was measured by the tentative diagnostic dose and degree method of China combined with the standard methods of WHO. Results In the area after more than 5 years mosquito control with the impregnated bed net method, An.sinensis had initial resistance to deltamethrin and permethrin. However, An.anthropophagus did not be captured. In the area after less than 5 years mosquito control with the impregnated bed net method, both An. sinensis and An.anthropophagus were susceptive to deltamethrin and permethrin. Conclusion In this research, no resistance of An.anthropophagus to deltamethrin and permethrin has been found. An.sinensis has initial resistance to them. So we must pay more attention to monitor the resistance in future malaria control programs.