Objective To observe the effect of Xingding injection on renal fuction,blood Laminin(LN)and Collegen Ⅳ(C-Ⅳ)in chronic renal failure(CRF).Methods Effect of SerumCreatinine(SCR),BloodUreaNitrogen (BUN)、Hemoglobin(Hb)、ALT、24 hupq、Laminin(LN)、Collegen Ⅳ(C-Ⅳ)was observed in CRF pa- tients.Results Compared with the controlvalues,significantly decreased the level of Scr,BUN,LN,C-Ⅳ and 24 hupq(P

The number of root canals varies most in the areas of mandibular anterior teeth, maxillary and mandibular premolars, and premolars. Generally, The mandibular anterior teeth have one root canal, but data suggest that the anatomical morphology of the root canals of mandibular incisors varies greatly, which easily leads to the neglect of root canals in endodontic treatment. This report described the case of a mother and a daughter whose mandibular anterior teeth have two symmetrical root canals.

Objective To investigate the survival principle of SARS-conarovirus (SARS-Cov) in environments and to explore the inactivation effect of chlorine and chlorine dioxide on SARS-Cov with the aim of providing some basis for blocking its'main route of transmission. Methods SARS-Cov were added into the excrement samples, urine samples and other water samples and were disinfected by sodium chlorite and chlorine dioxide. The survival ability of SARS-Cov and the effects of those two disinfectants on the inactivation of SARS-Cov were observed. Results The survival of SARS-Cov changed with the variation of temperature in external environment.It was showed that SARS-Cov could only survive for 2 days in hospital sewage, domestic sewage and dechlorinated tap water, while 3 days in excrement samples, 14 days in physiological saline and 17 days in urine samples protected from light in vitro at 20 ℃ . At 4 ℃, SARS-Cov could survive for 14 days in all above water samples and 17 days in excrement samples and urine samples. Resistibility of SARS-Cov to disinfectants in sewage sample was lower than those of E. coli and f2 phage. At the same disinfectant dosage or residual chlorine concentration , chlorine had better inactivation effect than chlorine dioxide did.The residual chlorine concentration of more than 0.5 mg/L or chlorine dioxide concentration of more than 2.19 mg/L was enough for the complete inactivation of SARS-Cov in sewage, but not enough for E. coli and f2 phage. Conclusion The survival time of SARS-Cov was shorter and more susceptible to chlorine and chlorine dioxide in external environment.

Objective The aim of this study is to compare the gains accomplished from different tympanoplasty based upon a review of patient records.Methods The charts of 137 patients who underwent different tympanoplasty operations were reviewed.They were divided into three groups.Group I,26 cases(26 ears) of tympanoplasty with mastoidectomy;group II,61 cases(62 ears) of tympanoplasty with mastoidectomy;group III,48 cases(49 ears) of myringoplasty.The hearing outcomes after dry ear were analyzed.Nonparametric statistics was used to determine group differences.A probability value of P

Objective To study the distribution characteristics of TCM constitutions of hormone-dependent breast cancer patients; To provide references for TCM diagnosis and treatment.Methods TCM constitution differentiation in the 210 patients with hormone-dependent breast cancer were investigated by a questionnaire survey, which is made according to the Classification and Determination of TCM Constitutions invented by professor Wang Qi and four methods of diagnosis of TCM, with a purpose to investigate the correlation of TCM constitutions and TCM syndrome differentiation typing.Results There was statistical significance among TCM constitutions of the 210 patients with hormone-dependent breast cancer (P<0.001), with yin deficiency 43.8% (92 cases), qi stagnation 15.2% (32 cases), qi deficiency 11.4% (24 cases), blood stasis 7.6% (16 cases), and yang deficiency 6.7% (14 cases). There was statistical significance among TCM syndromes (P<0.001), with dysfunction of Chong and Ren channels 63.8% (134 cases), stagnation of liver qi syndrome 16.2% (34 cases), weakness of the spleen and stomach syndrome 11.4% (24 cases). There was statistical significance between dysfunction of Chong and Ren channels and correlation of TCM constitutions (P<0.001).Conclusion Most hormone-dependent breast cancer patients are accompanied with yin deficiency pattern and the syndrome differentiation typing of dysfunction of Chong and Ren channels. There is correlation between dysfunction of Chong and Ren channels and TCM constitutions.

Allelic Imbalance ; Genetic Techniques ; Humans

Objective To investigate the infiltration of dendritic cell (DC) in Vocal cord polyp, laryngeal leukopla-kia and glottic squamous cell carcinoma,and to observe laryngeal mucosal lesions of the state of the immune microenviron-ment, and to research significance of DC on the development of glottic squamous cell carcinoma. Methods The infiltration of S-100+and CD83+DC in 20 cases of Vocal cord polyp, 47 cases of laryngeal leukoplakia, 45 cases of glottic squamous cell carcinoma tumor and 20 cases of laryngeal normal mucosa were examined using immunohistochemistry. Results The num-ber of S-100+and CD83+DC were significantly higher in glottic squamous cell carcinoma, laryngeal leukoplakia and vocal cord polyp than that in laryngeal normal mucosa (P<0.05). The number of S-100+and CD83+DC were higher in laryngeal leukoplakia than that in glottic squamous cell carcinoma (P<0.05). The number of S-100+and CD83+DC in mild dysplasia and moderate dysplasia were less than that in severe atypical hyperplasia (P<0.01). In glottic squamous cell carcinoma, S-100+ and CD83+DC with poor-differentiated was significantly less than that with well-differentiated (P < 0.01). Conclu-sion Changes in the number of dendritic cell was found in vocal cord polyp, laryngeal leukoplakia and glottic squamous cell carcinoma, which indicated that there were an abnormal immune status. Changing of dendritic cell in laryngeal mucosa plays an important role in laryngeal cancer development.

Objective To explore the best serum-free cultural way in neural stem cells from the hippocampus of the newborn rats and observe the characteristics of growth,proliferation,and induced differentiation of neural stem cells.Methods The neonatal rats′ hippocampus tissues were dissociated mechanically.The cells were cultured in the serum-free cultural medium which were added separately basic fibroblast growth factor(bFGF),epithelium growth factor(EGF),bFGF+EGF by suspended cultural way.The neural stem cells were identified by nestin immunofluorescence.After induced differentiation of embryonic cow serum,the differentiated cells were identified.Results The serum-free medium added bFGF and EGF could perfectly induce neural stem cells to proliferate and the differentiated cells expressed the specific antigen of neurons,astrocytes and oligodendrocytes.Conclusion The serum-free medium with bFGF and EGF can be used to culture neural stem cells from hippocampus tissue of rats in vitro.

Objective:To investigate the rationality of anti-fungal agents for deep fungal infection used in one hospital and the effects of national rectification of antimicrobial drugs. Methods: The retrospective analysis method was used to survey the inpatients administrated anti-fungal agents for deep fungal infection from April 2010 to Mard 2012, and assessed the rationality. Results:The ir-rational utilization of anti-fungal agents for deep fungal infection included loading dosage lack during the treatment,inappropriate loading dosage and administration frequency. The irrational utilization of anti-fungal agents for deep fungal infection was decreased significantly (P<0. 05)after the national rectification of antimicrobial drugs. Conclusion:After the national rectification of antimicrobial drugs, the hospital can amtrol the irrational use of anti-fungal agents to some extent, while still needs more management and education.

Objective The aim of this study is to establish a practical VX2 tumour model less than or equal to 10 mm from large blood vessel(as standard) for HIFU ablation .Methods 15 New Zealand rabbits were involved ,VX2 tumour blocks were inoculated near postcava through spine path ,and tumour rate was observed two weeks later by anatomy and MRI .Results Three experimental rabbits did not survive ,all the rest of the 12 into the tumour ,assembly tumour rate was 100% (12/12);The tumour rate reaching the standard accounted for 75% (9/12) ,the average distance between the tumour and the inferior vena cava was (5 .6 ± 3 .4)mm . Conclusion It is feasible to establish the VX2 tumor model less than or equal to 10 mm from large blood vessel through spine path .