1.Clinical observation on treatment of non-gonococcal cervicitis by integrative medicine.
Yu-Ning WU ; Yan JIN ; Ling-Yun PU
Chinese Journal of Integrated Traditional and Western Medicine 2005;25(4):362-364
OBJECTIVETo explore the integrative medicinal therapy for non-gonococcal cervicitis (NGC) in order to elevate the therapeutic effect for patients treated in vain after long-term application of antibiotics.
METHODSFifty patients with NGC were treated with Qingyuan decoction combined with antibiotics, and other 46 patients were treated with antibiotics alone for control.
RESULTSThe cure rate and significant effective rate was 90.0% (45/50) and 65.2% (30/46) in the treated group and the control group, it was significantly different between the two groups (chi2 = 9.58, P < 0.01). The disappearance rate of symptom was 88.1% and 41.4% in the two group after treated for two weeks respectively, and it was also significantly different (chi2 = 12.42, P < 0.01).
CONCLUSIONSThe therapeutic effect of NGC treated by sensitive antibiotics combined with Qingyuan decoction is better than that treated with western medicine only.
Adult ; Anti-Bacterial Agents ; therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Middle Aged ; Phytotherapy ; Uterine Cervicitis ; drug therapy ; microbiology
2.Transfection of embryonic stem cells with green fluorescent protein gene and their differentiation into neural cells
Zhi-yan, SHAN ; Jing-ling, SHEN ; Lei, LEI ; Yan-ning, XU ; Lian-hong, JIN
Chinese Journal of Endemiology 2008;27(4):397-400
Objective To establish embryonic stem cells (ESC) that can express green fluorescent protein (CFP) and differentiate them into neurons. It would provide tagging neurons for clinical transplantation to cure neural system diseases. Methods ESC (R1) was transfeeted with a plasmid containing the GFP by electroporation. A transgeuic cell line was obtained after selection with G418. The ESCs were characterized by AKP staining. Monolayer differentiation method was used to induce neural differentiation derived from GFP-ESC and immunofluorescence method was used to identify Tuj1 positive cells. Results There was no significant difference(X2=3.14,P0.05) in transfect rates between liposome and electroporation (65% vs 79%). The AKP staining of GFP-ESC was positive. GFP-ESC could be differentiated into neural cells. Conclusions These results show that ESC expressing GFP has been estabhshed, which can be differemiated into neurons.
3.Effects of Neuromuscular Electrical Stimulation Combined with Strength Training on Motor Function in Children with Spastic Cerebral Palsy
hui-ci, LIANG ; kai-shou, XU ; lu, HE ; jin-ling, LI ; jian-ning, MAI
Journal of Applied Clinical Pediatrics 2004;0(12):-
0.05).Compared with the CSS,GMFM and WV before treatment,there were statistically difference after 6 and 12 weeks treatment in two groups(Pa
4.Effects of transcutaneous electrical nerve stimulation on motor function in ambulant children with spastic cerebral palsy: a randomized trial.
Kai-shou XU ; Lu HE ; Jin-ling LI ; Jian-ning MAI
Chinese Journal of Pediatrics 2007;45(8):564-567
OBJECTIVETo investigate the effects of transcutaneous electrical nerve stimulation (TENS) on motor function in children with spastic cerebral palsy (CP).
METHODSAfter signing the informed consent, 78 children with CP, aged 45.6 +/- 8.5 months (36 to 58 months), were randomly divided into a TENS group (n = 40) and a control group (n = 38). All the subjects received standardized functional exercise program. In TENS group, 2 TENS devices were used and the surface electrodes were applied on the spastic musculotendinous and antagonist muscles in the affected lower extremity. TENS lasted for 20 min per session, 5 days weekly for 6 weeks. After 6 weeks, the functional exercise program was applied by the caregivers. Demographic data were recorded, including age, gender, number of the hemiplegic and diplegic CP, level of gross motor function classification system (GMFCS). Clinical assessments included the composite spasticity scale (CSS), D and E dimensions of the Gross Motor Function Measure (GMFM), and walking velocity was determined before treatment and at 6, 12 and 24 weeks after treatment.
RESULTSNo statistically significant differences were found in age, gender, number of the hemiplegic and diplegic CP, level of GMFCS, as well as clinical assessments (CSS, GMFM and walking velocity) before treatment between the 2 groups (P > 0.05). All the children showed a reduction of spasticity (CSS) after 6, 12 and 24 weeks of treatment (P < 0.05). When compared with the results obtained before treatment, the improvement of standing and walking (GMFM), walking velocity was statistically significant after 6, 12 and 24 weeks of treatment (P < 0.05). Furthermore, the differences of CSS, GMFM and walking velocity between the two groups at 6, 12 and 24 weeks examination were also statistically significant (after 24 weeks of treatment: t value was 8.96, 3.14 and 2.35, P < 0.05, respectively).
CONCLUSIONWhen compared with the control group, 6 weeks of TENS treatment on the affected lower extremity was more effective in terms of reducing spasticity and improving functional performance in standing, walking, and walking velocity in ambulant children with the spastic CP.
Cerebral Palsy ; therapy ; Child ; Electric Stimulation Therapy ; methods ; Female ; Gait Disorders, Neurologic ; therapy ; Hemiplegia ; therapy ; Humans ; Male ; Muscle Spasticity ; therapy ; Transcutaneous Electric Nerve Stimulation ; methods ; Treatment Outcome
5.Sappanone A attenuates renal ischemia-reperfusion injury in rats by regulating JNK signal pathway
Tai-wei JIN ; Xiao-ning GAO ; Wen-lin SONG ; Yan-yan WANG ; Lin SUN ; Ling-hong LU
Acta Pharmaceutica Sinica 2024;59(6):1639-1646
This study aimed to investigate the role and mechanism of sappanone A (SA) in regulating renal ischemia-reperfusion injury (IRI) in rats. The animal experiment has been approved by the Ethics Committee of Suzhou Wujiang District Children's Hospital (approval number: 2022010). First, hematoxylin-eosin (H&E) staining was used to evaluate the effects of SA on IRI, and renal damage was scored. Serum creatinine (SCr), blood urea nitrogen (BUN) and cystatin C (Cystatin C) were analyzed. The effect of sappanone A on the apoptosis of renal tubular epithelial cells induced by IRI was analyzed by TUNEL staining. Protein expression levels of p-JNK/JNK, p-ERK/ERK, Bcl2, Bax and cleaved-caspase 3 in renal tissues were detected by Western blot. Finally, H&E staining, serological analysis, TUNEL staining and Western blot were used to determine whether JNK activator anisomycin could reverse the effect of SA on IRI in rats. The results showed SA significantly reduced the renal tubule injury caused by ischemia-reperfusion, and decreased the level of SCr, BUN and Cys C in serum. TUNEL staining showed that SA significantly reduced the apoptosis of renal tubular epithelial cells induced by IRI. Western blot analysis of kidney tissue showed that SA significantly promoted the expression of apoptosis inhibiting protein Bcl2 and inhibited the expression of apoptosis-promoting proteins Bax and cleaved-caspase 3. Further analysis elucidated that SA did not affect the phosphorylation of ERK but decreased the phosphorylation of JNK. Finally, H&E staining, serological analysis, TUNEL staining and Western blot confirmed that JNK activator anisomycin could reverse the alleviating effect of SA on IRI in rats. The above findings suggest that SA could alleviate IRI in rats by inhibiting JNK phosphorylation.
6.Clinical Features and Prognostic of Patients with Primary Central Nervous System Lymphoma
Li-Tian ZHANG ; Cui-Cui LI ; Qi-Qi JIN ; Hao-Yun JIANG ; Ning-Ning YUE ; Peng-Yun ZENG ; Ling-Ling YUE ; Chong-Yang WU
Journal of Experimental Hematology 2024;32(3):723-732
Objective:To explore the clinical features and prognosis of patients with primary central nervous system lymphoma(PCNSL).Methods:A retrospective analysis was performed on the relationship between clinical features,treatment regimen and prognosis in 46 newly diagnosed patients with primary central nervous system lymphoma who were diagnosed and treated in The Second Hospital of Lanzhou University from January 2015 to September 2022.Fisher's exact probability method was used to analyze the differences in clinical data of different subgroups.Kaplan-Meier survival curve was used to analyze the overall survival rate and progression-free survival rate of patients with different treatments,and the factors influencing survival were analyzed.Results:Among 46 patients with PCNSL,which pathological type were diffuse large B-cell lymphoma(DLBCL).There were 26(56.5%)cases of male and 20(43.5%)of female,with a median age of 54(17-71)years.In Hans subtypes,14 cases(30.4%)of GCB subtype,32 cases(69.6%)of non-GCB subtype.32 cases(69.6%)of Ki-67 ≥80%.Among 36 patients who completed at least 2 cycles of treatment with follow-up data,the efficacy evaluation was as follows:overall response rate(ORR)was 63.9%,complete response(CR)rate was 47.2%,17 cases of CR,6 cases of PR.The 1-year progression-free survival rate and 1-year overall survival rate was 73.6%and 84.9%,respectively.The 2-year progression-free survival rate and 2-year overall survival rate was 52.2%and 68.9%,respectively.The ORR and CR rate of 17 patients treated with RMT regimen was 76.5%and 52.9%(9 cases CR and 4 cases PR),respectively.Univariate analysis of 3 groups of patients treated with RMT regimen,RM-BTKi regimen,and RM-TT regimen as first-line treament showed that deep brain infiltration was associated with adverse PFS(P=0.032),and treatment regimen was associated with adverse OS in PCNSL patients(P=0.025).Conclusion:Different treatment modalities were independent prognosis predictors for OS,the deep brain infiltration of PCNSL is a poor predictive factor for PFS.Patients with relapse/refractory(R/R)PCNSL have a longer overall survival time because to the novel medication BTKi.They have strong toleration and therapeutic potential as a first-line therapy for high-risk patients.
7.The retrospective study of transjugular intrahepatic portosystemic shunt with covered or uncovered stents in patients with portal hypertension.
Xing-jiang WU ; Jian-min CAO ; Jian-ming HAN ; Qian HUANG ; Ning LI ; Jie-shou LI
Chinese Journal of Surgery 2010;48(2):83-87
OBJECTIVETo retrospectively compare the clinical outcome in patients with portal hypertension treated with transjugular intrahepatic portosystemic shunt (TIPS) using Fluency stent-graft (PTFE-covered stents) or bare stents.
METHODSApproval of study and treatment protocol and waiver of informed consent for the retrospective study were obtained from institutional review board. Informed consent was obtained from each patient before procedure. Sixty consecutive patients with portal hypertension treated with TIPS from April 2007 to April 2009 were included. TIPS creation was performed with Fluency stent-graft in 30 patients (group A) and with bare stents in 30 patients (group B). Liver function, TIPS patency and clinical outcome were evaluated every 3 months.
RESULTSDuring hospitalization, there was no hepatic encephalopathy and recurrency of variceal bleeding.Acute shunt occlusion was observed in one patient with group A and another patient with group B.Follow-up was performed with average time of (6.2 +/- 3.9) months in group A and (8.3 +/- 4.4) months in group B. The rates of recurrent bleeding, acute shunt occlusion, hepatic encephalopathy and death were 3.3% and 20.0%, 0 and 30.0%, 16.7% and 20.0%, 0 and 13.3% in group A and B. The rates of recurrent bleeding, acute shunt occlusion and death in group A was lower than those in group B. There was no difference of hepatic encephalopathy between group A and B. The decrease of portal pressure and portosystemic pressure gradient, and the increase of portal flow and shunt flow in group A were higher than those in group B. There were no difference of liver function, ammonia and MELD between group A and B.
CONCLUSIONSFluency stent-graft is safe and effective in TIPS creation, with high patency rate. Covered-stent can improve the clinical outcome of portal hypertension.
Adult ; Aged ; Female ; Follow-Up Studies ; Humans ; Hypertension, Portal ; surgery ; Male ; Middle Aged ; Portasystemic Shunt, Transjugular Intrahepatic ; instrumentation ; Retrospective Studies ; Stents ; Treatment Outcome ; Young Adult
8.Mutation analysis of GCDH gene in eight patients with glutaric aciduria type I.
Jing CHEN ; Zhao-xia WANG ; Jin-li ZHANG ; Yan-ling YANG ; Jing CHEN ; Yi-ning HUANG
Chinese Journal of Medical Genetics 2011;28(4):374-378
OBJECTIVETo investigate the mutations of glutaryl-CoA dehydrogenase (GCDH) gene in patients with glutaric aciduria type I(GA-1).
METHODSGenomic DNA was extracted from peripheral blood cells of the eight probands with GA-1 who were diagnosed by urine and blood analyses. By PCR and direct sequencing, all 11 exons and their flanking sequences of the GCDH gene were examined. Mutation search was also performed in some of their family members.
RESULTSAmong the eight patients diagnosed by metabolic screening, seven patients belonged to classical infantile-onset. One patient, however, was adult-onset, who was admitted to the hospital because of suffering from ischemic cerebral stroke. The GCDH gene mutations were identified in all the eight probands with GA-1: five of them had compound heterozygous mutations, while the other three harbored only one heterozygous mutation. Totally, nine different mutations of the GCDH gene were identified in the eight probands, four of them were novel, i.e., c.148T>C, c.371G>A, 909delC and c.263G>A.
CONCLUSIONGCDH gene mutations are identified in 8 patients with GA-1 in mainland China, including one adult patient with late onset. Four novel mutations of GCDH gene are found which expanded the mutational spectrum of the GCDH gene.
Adult ; Amino Acid Metabolism, Inborn Errors ; enzymology ; genetics ; Amino Acid Sequence ; Animals ; Base Sequence ; Brain Diseases, Metabolic ; enzymology ; genetics ; DNA Mutational Analysis ; Exons ; genetics ; Female ; Glutaryl-CoA Dehydrogenase ; chemistry ; deficiency ; genetics ; Humans ; Infant ; Male ; Molecular Sequence Data
9.Inhibitory effect of emodin on proliferation of human lung adenocarcinoma cells in vitro.
Jia-ning LI ; Fu-zhen LU ; Jin-ling XIAO ; Yongxia BAO
Chinese Journal of Integrated Traditional and Western Medicine 2005;25(7):640-642
OBJECTIVETo investigate the anti-tumor mechanisms of emodin on human lung adenocarcinoma cell line Anip973 (LACC).
METHODSThe influence of emodin of different concentration at different time on LACC proliferation were determined and compared using MTT colorimeric assay and cell growth curve assay. And the cell apoptotic rate was determined by flow cytometry and analyzed with electronic microscope.
RESULTSIn a certain range, the higher concentration and longer acting time of emodin could induce the stronger inhibitory effect on tumor cell growth and the higher apoptotic rate. The proliferation inhibitory rate reached 90% after LACC being treated with emodin 60 micromol/L for 72h.
CONCLUSIONEmodin can significantly inhibit proliferation of human LACC, showing dose-effect and time-effect relationship.
Adenocarcinoma ; pathology ; Antineoplastic Agents, Phytogenic ; pharmacology ; Apoptosis ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Dose-Response Relationship, Drug ; Emodin ; pharmacology ; Humans ; Lung Neoplasms ; pathology
10.Evaluation of cardiac structure and function by cardiac magnetic resonance imaging in healthy volunteers.
Lu ZHOU ; Zheng-Yu JIN ; Zhu-Hua ZHANG ; Yi-Ning WANG ; Ling-Yan KONG ; Lan SONG ; Li-Ren ZHANG
Acta Academiae Medicinae Sinicae 2009;31(2):210-214
OBJECTIVETo evaluate the role of cardiac magnetic resonance (CMR) sequence in assessing the cardiac structure and function.
METHODSA total of 36 healthy volunteers were examined with fast imaging employing steady-state acquisition cine MR sequence. All the MR images were processed on Report Card software to test the cardiac dimensions and function. The relationships between the CMR results and the ultrasonic cardiography (UCG) results were analyzed.
RESULTSCMR analysis showed that interventricular septum thickness, left ventricle posterior wall thickness, end diastolic dimension of left ventricle, and end systolic dimension of left ventricle were (9.2 +/- 2.1), (8.0 +/- 2.1), (48.6 +/- 5.5), and (30.3 +/- 5.3) mm, respectively. Major dimension of right ventricle, minor dimension of right ventricle, index of major, dimension of right ventricle and index of minor dimension of right ventricle were (69.2 +/- 9.7), (30.6 +/- 6.6) mm, and (39.4 +/- 5.5), (18.1 +/- 3.4) min/m2, respectively. The left ventricle indicators above measured by CMR correlated with those by UCG (r = 0.843, 0.784, 0.686, 0.730, all P < 0.01). CMR analysis showed that left ventricular end diastolic volume, right ventricular end diastolic volume, left ventricular end systolic volume, and right ventricular end systolic volume were (93.6 +/- 17.2), (108.6 +/- 28.2), (39.5 +/- 13.0), and (45.6 +/- 15.1) ml, respectively. The left ventricular ejection fraction and right ventricular ejection fraction were (58.5 +/- 8.19)% and (58.2 +/- 7.4)% , respectively. Left ventricular ejection fraction measured by CMR was correlated with that (64.1 +/- 6.8)% by UCG (r = 0.75, P < 0.01).
CONCLUSIONSCMR sequence with standard location is well applicable for accurate measurement of left and right ventricle dimensions and function, especially for right ventricle. CMR can be used to diagnose the heart disease and monitor the efficacy.
Adult ; Aged ; Echocardiography ; Female ; Heart ; anatomy & histology ; physiology ; Humans ; Image Processing, Computer-Assisted ; Magnetic Resonance Imaging, Cine ; methods ; Male ; Middle Aged ; Reference Values ; Stroke Volume ; Ventricular Function