Adenosine Triphosphatases ; metabolism ; Animals ; Antioxidants ; metabolism ; Muscle, Skeletal ; drug effects ; enzymology ; Rats ; Sulfinic Acids ; pharmacology

The innate immune response may be activated quickly once the organism is invaded by exotic pathogens. An excessive immune response may result in inflammation and tissue damage, whereas an insufficient immune response may result in infection. Nervous system may regulate the intensity of innate immune responses by releasing neurotransmitters, neuropeptides and hormones. Compared with the complicated neuro-immune system in mammals, it is much simpler in Caenorhabditis elegans. Besides, C. elegans is accessible to genetic, molecular biology and behavioral analyses, so it has been used in studies on neuro-immune interactions. It has been revealed recently in the studies with C. elegans that the neuronal pathways regulating innate immune responses primarily include a transforming growth factor-β (TGF-β) pathway, an insulin/insulin-like growth factor receptor (IGF) pathway and dopaminergic neurotransmission. Since these pathways are evolutionally conservative, so it might be able to provide some new ideas for the research on neuro-immune interactions at molecular levels. The recent progress in this field has been reviewed in present paper.

A comparison of the number of clinicians per 1000 residents in the province,and analysis of their distribution and characteristics identified an imbalance of such resources between regionsand between urban and rural areas.A further analysis of the payment flow of medical insurance fund of the counties and districts probed into the deployment efficiency of clinical in the province,holding that the hck of a human resources deployment and guidance mechanism for clinical resources is key to toor healthcare equity and difficulties in accessibility of healthcare service.

ObjectiveTo investigate the sleep status and effect of night shift work on sleep in nurses.MethodsSleep characteristics and qualities of 348 nurses, 50 communication persons and 100 night-guards were analyzed with Pittsburgh Sleep Quality Index (PSQI), and insomnia was diagnosed based on DSM-IV criteria.Results71.86% of nurses had bad sleep quality (PSQI>7); the rate of insomnia was 38.22%. The same data were 74% and 48% respectively in communication persons, and 26.76% and 7% in night-guards. The sleep quality of nurses was related to age and years of night shift work. The sleep quality of nurses was similar to communication persons (P>0.05), but significantly different from that of night-guards (P<0.01).ConclusionShift work manner influences sleep status of nurses and makes them having disturbances on falling sleep time, the time of sleeping, sleep efficiency and daytime function. But it doesn't need medication.

Objectiv To observe the process of wound healing and investigate the characteristics of Treg/Th17 balance in rats with combined wound and 5 Gy whole-body γ -radiation (CWR) for exploring the interrelationship between wound healing and the immune balance of Treg/Th17. Methods Sixty-five female Wistar rats (clean grade) were randomly divided, depending on weight, into normal control group (normal), wound only group (MW) and wound plus 5 Gy whole body γ -radiation group (RW). The wound area was measured with image analyzer, the peripheral white blood cells (WBC) and lymphocytes (LYM) were counted with automatic cytometry, and the percentage of splenic regulatory T cells (Tregs) and Th17 cells was analyzed by flow cytometry at different time points post CWR. Results (1) The residual wound area was much greater in RW group than in MW group (P<0.01) 7d to 21d after CTR, the wound surface in MW group almost healed on 21d, while that in RW group healed on 28d post CTR. (2) The peripheral WBC and LYM counts were significantly lower in MW group than in normal group (P<0.05) 1d to 7d after CWR, and in RW group than in normal and MW group 1d to 14d after CWR. Furthermore, the WBC count was still obviously lower in RW group than in MW and normal groups 21d to 28 d after CWR. (3) The percentage of splenic Tregs was significantly higher in RW group than that in normal and MW groups (P<0.01) at 3d and 7d after CWR, and returned to normal level at 28 d after CWR. (4) The percentage of Th17 cells was notably higher in RW group than in normal and MW groups (P<0.01) on the first day, reached the peak value on 3 d, and returned to normal level on 28 d after CWR. (5) The ratio of Treg/Th17 was distinctly lower in RW group than in normal and MW groups from 1d to 21d, and approximated normal level on 28d after CWR, indicating that 5Gy whole-body γ-radiation could induce Treg/Th17 imbalance in wounded rats. Conclusions 5 Gy whole-body γ-radiation may delay the process of wound healing in rats. Radiation-induced changes in Tregs and Th17 may lead to the disequilibrium of Treg/Th17, which may be an important factor for delayed wound healing in CWR.

The innate immune response may be activated quickly once the organism is invaded by exotic pathogens. An excessive immune response may result in inflammation and tissue damage, whereas an insufficient immune response may result in infection. Nervous system may regulate the intensity of innate immune responses by releasing neurotransmitters, neuropeptides and hormones. Compared with the complicated neuro-immune system in mammals, it is much simpler in Caenorhabditis elegans. Besides, C. elegans is accessible to genetic, molecular biology and behavioral analyses, so it has been used in studies on neuro-immune interactions. It has been revealed recently in the studies with C. elegans that the neuronal pathways regulating innate immune responses primarily include a transforming growth factor-β (TGF-β) pathway, an insulin/insulin-like growth factor receptor (IGF) pathway and dopaminergic neurotransmission. Since these pathways are evolutionally conservative, so it might be able to provide some new ideas for the research on neuro-immune interactions at molecular levels. The recent progress in this field has been reviewed in present paper.

OBJECTIVETo explore effects and action mechanism of warm moxibustion on regulation of blood lipids and anti-atherosclerosis.

METHODSForty-one male Japanese big-ear white rabbits were randomly divided into a blank group (10 rabbits), a model group (10 rabbits), a moxibustion group (10 rabbits) and a medication group (11 rabbits). Normal diet was applied in the blank group while high-cholesterol diet combined with injection of bovine serum albumin were applied in the rest groups to establish rabbit model of atherosclerosis. After establishment, the model group was not intervened and warm moxibustion was applied in the moxibustion group at "Zusan-li" (ST 36) and "Shenque" (CV 8), 10 min per acupoint per day for continuous 4 weeks. The medication group was treated with intragastric administration of lovastatin capsule (3.6 mg/kg) for continuous 4 weeks. The level of blood lipids, such as total cholesterol (TC), triglyceride (TG), and content of CD40 ligand (CD40L), soluble CD40 ligand (sCD40L) and expression of nuclear factor NF-kappaB were tested after 4 weeks.

RESULTSCompared with the model group, the moxibustion group and medication group could effectively reduce the contents of TC and low density lipoprotein (all P < 0.05), lower the level of sCD40L [(8.310 +/- 1.221) ng/mL in the model group, (7.097 +/- 0.846) ng/mL in the moxibustion group and (7.354 +/- 0.631) ng/mL in the medication group], reduce expression of CD40L [(0.235 +/- 0.179) mm2 in the model group, (0.072 +/- 0.079) mm2 in the moxibustion group and (0.039 +/- 0.015) mm2 in the medication group] and NF-kappaB [(0.145 +/- 0.052)mm2 in the model group, (0.052 +/- 0.012) mm2 in the moxibustion group and (0.036 +/- 0.013) mm2 in the medication group], indicating the significant difference (P < 0.05, P < 0.001). There was no statistical difference between the moxibustion group and medication group (all P > 0.05).

CONCLUSIONThe warm moxibustion has great effect on regulation of blood lipids and anti-atherosclerosis, in which lowering expression of CD40-CD40L could be one of possible mechanisms to take effect of anti-atherosclerosis.

Animals ; Atherosclerosis ; blood ; therapy ; CD40 Antigens ; blood ; CD40 Ligand ; blood ; Humans ; Lipids ; blood ; Male ; Moxibustion ; methods ; Rabbits

Objective To study the role of hematopoietic growth factor(HGF)of placenta chorionic villus in fetal hematopoiesis during embryo ontogeny by observation of the appearance time and the content changes with the fetal growth, which was compared with HGF in cord blood. Methods Thirty embryo villus (2 g each) and 30 cord blood (2 mL each) were collected separately from early pregnant stage(6- 8 weeks), middle pregnant stage(16-22 weeks)and late pregnant stage (37-42 weeks). The levels of HGF were detected by enzyme - linked immunosorbent assay. Results HGF were produced on the early pregnant stage and the content of FL-T3,IL-3 increased gradually.There were significantly differences at different stages(P

BACKGROUND:Understanding the difference of miRNA-34s expression in normal tissue and tumor tissue wil contribute to screen out a miRNA with high sensitivity as the specific tumor molecular marker.
OBJECTIVE:To investigate the differential expression of miRNA-34s (miR-34a/b/c) between normal skin and keloid tissue using real-time fluorescent quantitative PCR, and to evaluate the role and mechanisms of miRNA-34s in keloid formation and development.
METHODS:Ten cases of keloid tissue and two cases of normal skin tissue were col ected as specimens. Total RNAs were extracted from keloid and nomal skin tissue by Trizol method, and miRNA-34s were further isolated by Ambion’s miRNA Isolation Kit. Real-time fluorescent quantitative PCR was applied to verify expression levels of microRNA-34s (miR-34a/b/c) in keloid tissue and normal skin tissue.
RESULTS AND CONCLUSION:miRNA-34s (miRNA-34a/b/c) expression was down-regulated in keloid tissue compared with normal skin tissue (P<0.01). The findings showed that miRNA-34s (miRNA-34a/b/c) are involved in keloid formation and development, and down-regulation of the family member may result in neoplastic growth of keloid.

AIM: To investigate the effect of Qingrehuayuziyin Fang on lymphocyte apoptosis and expression of Fas and FasL in peripheral blood in the patients with systemic lupus erythematosus(SLE).METHODS: Eighty patients with SLE were selected and divided randomly into two groups,40 in the therapy group and 40 in control group respectively.Patients were get the treatment of Qingrehuayuziyin Fang combined hormone(prednisone) as therapy group and get hormone treating only as control group.After treating three months,lymphocyte were collected to be detected the rate of apoptosis by flow cytometer.Meanwhile,the expression of Fas and FasL were analyzed by RT-PCR.RESULTS: After three months treating,the rate of lymphocyte apoptosis was decreased significantly in therapy group compared with that in control group(P