Objective To investigate the feasibility of endothelial reconstruction in the injuried arterial wall by autologous endothelial cells transplantation. Methods New Zealand white rabbits (n=20) were subjected to bilateral iliofemoral artery balloon injury, Cultured autologous venous endothelial cells were immediately transplanted by balloon catheter into one vessel, whereas the contralateral artery received the medium only. In 10 rabbits, vessels were harvested 4 hours and 4 days after transplantation for analysis of endothelial coverage by scanning electron microscope (SEM); In 5 rabbits, the cultured endothelial cells were labeled with a fluorescent tracer before their introduction into the injured vessel, and 4 days after transplantation, vessels were harvested to obtain fluorescent imaging of the seeded endothelial cells; Another 5 rabbits were sacrificed 4 days after transplantation for Evans blue staining. Results Four hours after the operation, SEM demostrated that the endothelial layer in control vessels were denuded completely, whereas some round endothelial cells had adhered into the aterial wall in cell transplantation group; four days after cell transplantation, the transplanted cells had attached and spread in the injuried aterial wall by SEM, a number of fluorescent labeling endothelial cells were observed in the endothelial denuded aterial wall, the vessels that received the medium only were stained nearly completely by Evans blue, whereas in those vessels that received cell transplantation, about 60% area were not stained. Conclusion Autologus endothelial cells can be effectively transplanted into the injuried arterial wall by balloon catheter.

Objective To study the distribution characteristics of TCM constitutions of hormone-dependent breast cancer patients; To provide references for TCM diagnosis and treatment.Methods TCM constitution differentiation in the 210 patients with hormone-dependent breast cancer were investigated by a questionnaire survey, which is made according to the Classification and Determination of TCM Constitutions invented by professor Wang Qi and four methods of diagnosis of TCM, with a purpose to investigate the correlation of TCM constitutions and TCM syndrome differentiation typing.Results There was statistical significance among TCM constitutions of the 210 patients with hormone-dependent breast cancer (P<0.001), with yin deficiency 43.8% (92 cases), qi stagnation 15.2% (32 cases), qi deficiency 11.4% (24 cases), blood stasis 7.6% (16 cases), and yang deficiency 6.7% (14 cases). There was statistical significance among TCM syndromes (P<0.001), with dysfunction of Chong and Ren channels 63.8% (134 cases), stagnation of liver qi syndrome 16.2% (34 cases), weakness of the spleen and stomach syndrome 11.4% (24 cases). There was statistical significance between dysfunction of Chong and Ren channels and correlation of TCM constitutions (P<0.001).Conclusion Most hormone-dependent breast cancer patients are accompanied with yin deficiency pattern and the syndrome differentiation typing of dysfunction of Chong and Ren channels. There is correlation between dysfunction of Chong and Ren channels and TCM constitutions.

BACKGROUND: Human body is an irregular geometrical one, so it is very diffcult to measure its volume.OBJECTIVE: To establish multiple regression equations by taking body height and body mass as the independent variables and body volume as the dependent variable, calculate the body volume of female college students and select optimal regression equations.DESIGN: A single-sample univariate analysis.SETTING: Department of Physical Education of Lishui College, Department of Physical Education of Zhejiang University and Academy of police in Zhejiang. PARTICIPANTS: Eighteen female students aged 18-22 years were selected from Zhejiang Lishui College.METHODS: Both the body height and body mass indexes of the female students were measured with the nation-ratified constitutional test instrument, and the body volume index was measured with a self-made iron container with a diameter of 0.95 m and height of 1.20 m. There was a scale mark for height in the container, water was poured to a fixed height, then the student slowly immersed herself into the water completely and the height difference was recorded. Body volume (m3)=(0.95÷2)2×3.141 59×height difference. The measured data were statistically calculated. Regression equations were established by taking body height and body mass as the independent variables and body volume as the dependent variable,and the optimal regression equation was selected.MAIN OUTCOME MEASURES: The measured data of body height,body mass and body volume of female students and the calculated results of the regression equations were observed.RESULTS: The measured data of body height, body mass and body volume of 18 female students all entered into the in result analysis ① A regression equation in two unknowns for calculating body volume was established: (y)=-0.031 016+0.000 761×body mass+0.000 267×body height. ②A regression equation in one unknown for body volume and its optimal selection: The linear equation was y=0.001×body mass+0.000 8; the logarithm equation was y=0.005 1Ln (body mass)-0.15; the power equation was (y)=0.001×body mass0.9909; the exponent equation was y =0.0192×e0.0188x.and the multiple correlation coefficient R2=0.9497-0.9591, all were close to 1, indicating that the body volume predicted by models was highly correlated with the actual one (r ＞ r0.001(18-2), P ＜ 0.001), the predicted values of the 4 models were not different from the actual one. ③ Analyzing from the simplicity of calculation and the economic way of index measurement, Logarithm equation is the best in the 5 regression equations. ④ The body volume covered body shape, physical function and Physique.CONCLUSION: The index of body volume is one of the important indexes, which cannot be neglected in the study of Physique. Analyzing from the simplicity of calculation and the economic way of index measurement, Logarithm equation is the best.

Objective To explore the change of serum osteopontin levels in preoperative and postoperative patients with non-small cell lung cancer (NSCLC) and its clinic significance.Methods The levels of serum osteopontin were detected by ELISA.Results In 88 NSCLC patients,serum osteopontin levels were significantly higher than in benign pulmonary disease and healthy control group(P＜0.05.P＜0.01.respectively) There was no difference between benign pulmonary disease and healthy control group(P＞0.05).Serum osteopontin levels were related to clinical stage and lymph node metastasis,and the levels of serum osteopontin were lower in postoperative patients than in preoperative cases(P＜0.05).For NSCLC.the detection of osteopontin had a sensitivity of 84.1%,a specificity of 92.5%,and an accuracy of 88.1%. Conclusion Increased serum levels of osteopontin may be useful as a helpful clinical biomarker to diagnose earlier,predict prognosis and differentiate from benign pulmonary disease.

Fifty four patients with chronic sinusitis (without nasal polyps) were randomly divided into two groups. Thirty patients in experimental group were treated with dirithromycin and 24 patients in control group were treated with clarithromycin. The doses was both 250 mg q. d. and the duration of treatment was 3to 6 months. The VAS scores of subjective symptoms (nasal obstruction, purulent nasal discharge,headache, smell worse ) and Lund-Mackay scores of objective signs (sinus CT ) in both groups were improved significantly compared with the baseline(P ＜0.05), and there were no statistical significances between two groups ( P ＞ 0. 05 ). The curative effect was positively correlated with the duration of treatment.The total effective rates were 73% and 67% and adverse reaction rates 7% and 21% in two groups,respectively (P ＞ 0. 05 ); but the adverse reactions in control group were more serious than those in experimental group. During the six-month follow-up, no recurrence and aggravation were observed. The results suggest that low-dose dirithromycin may be more suitable for long-term treatment of chronic sinusitis.

Objective To discuss the role of EPI-DWI unite ASSET in the detection of hepatic colorectal micrometastasis. Methods 29 patients with hepatic colorectal metastasis were examined with EPI-DWI unite ASSET and EPI-DWI. Results In a lesion detection analysis,the overall detection rate was 93% (58/63) for ASSET-EPI-DWI,and 81% (51/63) for diffusion-weighted MR imaging. ASSET and EPI-DWI was more sensitive than diffusion-weighted MR imaging(P = 0.01 1). Conclusion ASSET-EPI-DWI is superior to diffusion-weighted MR imaging in the detection of hepatic colorectal micrometastasis.

Humans ; Integrative Medicine ; Medicine, Chinese Traditional ; trends

Objective To investigate the effect of dexamethasone combined with oridonin on proliferation and apoptosis in multiple myeloma cells U266 and the related molecular mechanism. Methods Exponential phase of growth U266 cells were treated with different concentrations of oridonin combined with dexamethasone or alone. U266 cells treated by DMSO were taken as control group. The proliferation inhibitory ratios were measured by CCK-8 assay followed by 24 h, 48 h and 72 h. Apoptosis induction was assessed by using Annexin V-FITC kit. Real time PCR was used to examine the mRNA changes of Notch1, NF-κB/p65 and bcl-2. Western blot assay was applied to detect the protein expression of Notch1, cleaved Notch1, NF-κB/p65 and bcl-2. Results Compared with that in control group, proliferation in all the experimental groups was inhibited (P<0.05), and the apoptosis was promoted (P<0.05); especially the combination of dexamethasone and oridonin had a synergistic effect on the proliferation and apoptosis of U266 cells (P<0.05). The results of PCR and Western blot showed that after treatment of U266 cells with dexamethasone, the mRNA as well as their protein levels of NF-κB/p65 and bcl-2 were decreased compared with those in the control group (P<0.05). Moreover, the mRNA and protein expression of Notch1, cleaved Notch1, NF-κB/p65 and bcl-2 was obviously down-regulated in oridonin group and the combination group (P<0.05). Conclusion Combination of dexamethasone and oridonin can significantly increase the anti-tumor effect by inhibiting proliferation and inducing apoptosis of U266 cells, which may be related to the inhibition of the Notch1 pathway.

Background:Irritable bowel syndrome(IBS)is a commonly seen functional intestinal disorder. Dietary factors are important factors affecting the abdominal symptoms of IBS patients. Aims:To investigate the effect of dietary factors on abdominal symptoms of IBS and the application of dietary adjustment strategy in patients lived in Xian,Shaanxi Province and the surrounding areas. Methods:Fifty-four outpatients with abdominal complaints who met the Rome Ⅲ criteria of IBS from Feb. 2015 to Jul. 2015 at the Second Affiliated Hospital of Xian Jiaotong University were enrolled. Twenty healthy volunteers were served as controls. All subjects completed a questionnaire containing issues mainly on IBS symptoms, eating habits,dietary factors causing abdominal symptoms,and the patients’coping styles. Results:Compared with healthy controls,more rice and less spicy food,fatty food,raw/ cold food,acidic beverages and alcohol drinking were observed in diet of IBS patients(P all < 0. 05). Abdominal symptoms of IBS patients could be caused or exacerbated predominantly by cold food(55. 6% ),fatty food(55. 6% ),raw food(48. 1% ),meat(48. 1% )and spicy food (40. 7% ). More than half of the IBS patients(63. 0% ) adopted an elimination diet to minimize their abdominal symptoms. Spicy food(59. 3% ),raw food(48. 1% ),cold food(44. 4% ),fatty food(44. 4% )and meat(37. 0% ) were the most frequently eliminated classes of food. Conclusions:Dietary factors can affect the abdominal symptoms of IBS in patients lived in Xian,Shaanxi Province and the surrounding areas and elimination diet based on foods they perceive as problematic is widely used. The classes of food and eating habits causing abdominal symptoms are quite different between domestic and overseas IBS patients.

Objective In problem-based learning, students are often found difficult to con-struct medical knowledge systematically and transfer knowledge to solve new problems. In face of this challenge, this study aims to investigate the effect of visualization tool-supported online problem-based learning on medical students' clinical diagnostic expertise development. Methods A controlled study was conducted and 52 medical students were randomly assigned into experimental group (using visual-ization tool-supported online PBL environment for learning) and control group (using online PBL envi-ronment for learning without visualization tool support). Participants were asked to complete the diag-nostic analysis of three kidney problems according to the requirements of the learning environments and to provide feedback of online learning experience afterwards. Paired-sample t test and one-way analysis of vonriance were used to analyze both group's case 1 and case 3 on line learing scores. Results The results revealed that the experimental group had significant improvement in online learning performance [case 1: (1.47 ±0.54), case 3: (2.14 ±0.55), P=0.015], while the control group had no significant improvement [case 1:(1.57±0.67), case 3:(1.66±0.49), P=0.234]. Early performance of online learning and group factor had interative effects (F=7.266, P=0.013). Conclusions The findings suggest that visualization tool-supported online PBL environment can facilitate medical student clinical diagnostic expertise development effectively.