In order to make a rational and effective cure measure for lung cancer,people need to establish suitable animal model.Spontaneous or induced model needs long time to form tumor and the rate is not steady.Xenograft mouse model is widely used in laboratories because of its steady tumor formation rate and less time in oncogenesis.Engineered mouse model which is based on genetic technology not only helps us to understand pathological process of lung cancer,but also can provide an ideal preclinical model of targeted therapy experiment,and it will be the important development direction of lung cancer animal model.

Objective To observe the effects of Lentinan(LNT) combined with Cisplatin (DDP) on the apoptosis induction in mice bearing Lewis lung carcinomas (LLC). Methods Lewis lung carcinoma cells (1?107) were subcutaneously inoculated into BALB/c mice. Mice were randomized into control groups and treatment groups. Observe the morphologic views under light microscope (LM) and electron microscope (EM). Results Compared with the control groups, the treatment groups were morphologic characteristic by relatively smaller in size, cell atypia were not evident, pathalogical karyokinesis were less interstitial blood vessel were fewer, while lymphocyte infiltrate into the interstitial. Apoptotic bodies could be seen under EM. Within all treatment groups, LNT+DDP group showed significant difference. Conclusions Lentinan combined with Cisplatin showed significant antitumor effects, and mechanism maybe inducing the tumor cell apoptosis and stimulate the proliferation of lymphocyte.

Objective To investigate the clinical significance of serum P-selection,TNF-α,hs-CRP,IL-1β, ICAM in patients with diabetic renal microangipathy, and the therapeutic mechanism of Simvastatin combined with aspirinon in treating renal microangipathy. Methods The serum levels of P-selection,TNF-α, hs-CRP, IL-1β, ICAM were determined by ELISA in all groups. Patients with diabetic renal microangipathy treated with simvastatin and as-pirinon. Results ① The serum levels of P-selection,TNF-α,hs-CRP,IL-1β,ICAM in DM groups were higher than those of normal controls(P<0.01). ②After treatment with simvastatin and aspirinon for 8 weeks in patients with di-abetic nephropathy,the serum levels of P-selection, TNF-α, hs-CRP, IL-1β, ICAM were all decreased significantly compared with those before treatment respectively (P<0.01). After treatment for 16 weeks the above indices re-mained decreased until treatment for 21 weeks (P<0.01). Conclusion ①The plasma levels of Poselection,TNF-α and hs-CRP, IL-1β, ICAM are correlated to the occurance and development of diabetic renal microangiopathy, becom-ing predicted factors in early stage of diabetic renal microangiopathy. ②The therapeutic mechanism of simvastatin and aspirinon in patients with diabetic nephropathy lies probably in the inhibition of these factors expression, thus di-rectly or indirectly affecting the prolffer-ation of mesangial cells and mesangial matrix.

Best vitelliform macular dystrophy ( BVMD ) is an autosomal dominant disease mostly caused by mutations in BEST1 gene. These mutations change the normal physiological functions of BEST1-encoded bestrophin-1 protein, and finally lead to a reduction of visual acuity. This review is composed of the following aspects: the structure and functions of BEST1 gene, the characteristics of the mutations, clinical features of BVMD, genotype-phenotype correlations as well as possible gene therapy. Our contribution serves for further research on BVMD and BEST1 gene.

Objective To investigate the effect of medium- and long-chain triacylglycerols on body fat accumulation in subjects with different levels of hypertriglyceridemia. Methods 112 subjects with hypertriglyceridemia were randomly divided into two groups: one group was demanded to adopt long-chain triacylglycerol (LCT) oil as their daily use and the other one to medium- and long-chain triacylglycerol (MLCT) oil for consecutive 8 weeks. Anthropometric measurements of body weight, body mass index (BMI), waist circumference (WC), hip circumference(HC), WHR (ratio of WC/HC), triceps skinfold thickness (TSF), subscapular skinfold thickness (SSF), body fat weight, body fat percentage, total fat area, visceral and subcutaneous fat area in abdominal and concentration of blood triglycerides (TG) were measured at the initial and final time of the study. In both groups, the cutpoint of TG 2.3 mmol/L was used to distinguish the different levels of hypertriglyceridemia. Results Compared with LCT group, body weight, BMI, WC, body fat weight, total fat area, visceral and subcutaneous fat area in abdominal in those of TG≤2.3 mmol/L in MLCT group were significantly decreased (P 2.3 mmol/L between the two groups. Furthermore, the concentrations of TG in hypertriglyceridemia subjects of different levels in MLCT group were both significantly lower than those in LCT group after 8 weeks (P 2.3mmol/L.

0.05)and the main pharmacokinetic parameters were close to each other.CONCLUSION:No stereoselective differences lies in the pharmacokinetics processe of amlodipine enatiomers in the body of rabbits.

Objective To observe the effects of exposure to infrasound on auditory function of guinea pigs as reflected by the brain stem response (ABR) and distortion product otoaeoustic emission(DPOAE).Methods Seventy-two Guinea pigs were used in this study,of them,12 served as controls and 60 were divided into 2 experi- mental subgroups.ABR and DPOAE were detected after exposure to infrasound stimulation at 16 Hz,90 dB or 16 Hz,130 dB for 2 hours a day for 1 ,7,14,21 and 28 days.Results The threshold of ABR after exposed to infrasound at 16 Hz,90 dB in 1 day and 28 days was higher than the controls (P0.05),but significant difference was observed with 16 Hz 130 dB infrasound exposure on the 21st and,28th days when compared with the controls (P

The oil content and fatty acid composition of Ganoderma lucidum collected from different producing areas, varieties, tissue types and growth periods were measured and analyzed. The results showed that the oil content was 23. 61%-34.17% in different domestic producing areas of China; the oil content of fruiting bodies from major varieties cultured in Zhejiang province were 0.81%-1.87%, wall-unbroken spores were 0.07%-0.24%, wall-broken spores were 27.54%-34.17%, so the oil content of wall-unbroken spores were much higher than fruiting bodies, and wall-breaking treatment would increase the oil extraction rate 150-340 times. G. lucidum spores oil was mainly composed of unsaturated fatty acid composition. oleic acid and linoleic content were 53.26%-58.16% and 10.69%-16.87% respectively. Fatty acid composition ratio of spores and fruiting bodies were significantly different by PLS-DA. Determining the composition of fatty acid, especially the content of oleic acid, stearic acid and palmitic acid, could identify the tissue types of G. lucidum products' sources. In addition, the study result showed that the spores and fruiting bodies collected in the first year contained richer oil and fatty acid than second year's samples from the same variety of G. lucidum.
Fatty Acids ; analysis ; Oils ; analysis ; Reishi ; chemistry

Cone-Beam Computed Tomography ; methods ; Humans ; Nasopharyngeal Neoplasms ; diagnostic imaging ; radiotherapy ; Radiotherapy Planning, Computer-Assisted ; Radiotherapy, Intensity-Modulated ; methods

OBJECTIVE:To establish a method for simultaneous determination of valsartan and hydrochlorothiazide in Valsar-tan hydrochlorothiazide tablets. METHODS:UPLC was adopted. The determination was performed on Phenomenex C18 column with mobile phase consisted of [0.1% phosphoric acid solution-acetonitrile(95 : 5,V/V)]-[0.1% phosphoric acid solution-acetonitrile (5 : 95,V/V)](gradient elution)at the flow rate of 0.25 mL/min. The detection wavelength was set at 272 nm,and the column tem-perature was 35 ℃. The sample size was 1.5 μL. RESULTS:The linear range were 8.1-324.2 μg/mL for valsartan(r=0.9999)and 1.2-50.1 μg/mL for hydrochlorothiazide(r=0.9999). The limits of quantitation were 0.24,0.04 ng,and the limits of detection were 0.06,0.01 ng. RSDs of precision,stability and reproducibility tests were less than 2.0%;recoveries were 97.69%-100.35%for valsartan(RSD=1.03%,n=9)and 98.27%-100.60% for hydrochlorothiazide(RSD=0.83%,n=9). CONCLUSIONS:The method is simple,rapid and accurate,and can be used for the simultaneous determination of valsartan and hydrochlorothiazide in Valsartan hydrochlorothiazide tablets.