Plants in Ainsliaea genus, belongs to Compositae family, are traditional Chinese medicine and widely used in folk. These plants contain various types of chemical components, and main components are sesquiterpene lactone and its glycosides. In addition, there are triterpenoids, flavonoids, steroids, phenolic acid, long chain fatty acid and volatile oils. Recently, much attention has been payed to varlous research of A. fragrans. This paper reviewed and summarized the chemical components to provide the theoretical basis for the use of Ainsliaea.
Asteraceae ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Molecular Structure

Objectives To compare total laparoscopic gastrectomy with intracorporeal hand-sewn Gl reconstruction and laparoscopy-assisted gastrectomy for gastric cancer. Methods Between July 2009 and July 2010, 21 patients of gastric cancer underwent total laparoscopic D2 radical gastrectomy with intracorporeal hand-sewn reconstruction and 28 did laparoscopy-assisted D2 radical gastrectomy in Xijing Hospital of Digestive Diseases. All patients were operated on by an experienced surgeon. Patient demographics, TNM stage, location of tumor, the intraoperative and postoperative details of the two groups were compared. Results In the 21 patients undergoing total laparoscopic gastrectomy, there were 15 of distal gastrectomy and 6 of total gastrectomy, compared with 21 and 7 in laparoscopy-assisted group. In total laparoscopic group, intracorporeal hand-sewn technique was used for gastro-jejunal and jejuno-jejunal (J-J)anastomosis, and 25 mm circular stapler was used for esophago-jejunal anastomosis. The operation time was significant longer in total laparoscopic group than in laparoscopy-assisted group of (279 ± 65 ) min vs.(232 ±40) min (P ＜ 0.05 ). No significant difference was observed between the two groups in proximal margin [(5.7 ± 1.5 )cm vs. (5.1 ± 1.4) cm, P ＞ 0.05] and distal margin [( 3.1 ± 0.9 )cm vs. ( 2.9 ±0.9) cm,P ＞0.05]. The iv narcotic use in laparoscopy-assisted group was 1.8 d but it was not used in total laparoscopic group. The first passing flatus was on day 3 in total laparoscopic group compared with 4.8 d in laparoscopy-assisted group. Both groups had 2 postoperative early complications, one intra-abdominal infection and one lung infection in total laparoscopic group compared with one wound infection and one lung infection in laparoscopy-assisted group. There was no anastomosis-related complications after 4 months of follow-up. Conclusions The operation time and postoperative early complication was acceptable for selected patients treated by total laparoscopic D2 radical gastrectomy with intracorporeal hand-sewn GI tract reconstruction in hands of experienced laparoscopic surgeon.

Objective To investigate the correlation of single nucleotide poly morphisms (SNP) of silent information regulator 1 (SIRT1) gene with age-related cataract (ARC) in Jiangsu Har population.Methods Population-based case-control study was conducted in 720 cataract cases and 701 healthy controls,who were selected from the database of Funing County of Northern Jiangsu and Binhu District of Wuxi city of Southern Jiangsu.Five SNPs of SIRT1 gene (rs2236319,rs1885472,rs10997868,rs2273773 and rs4746720) were genotyped by Taq-Man RT-PCR methods.Results The genotype of 2 SNPs in SIRT1 (rs2236319 and rs1885472) was AA/AG/GG and CC/CG/GG respectively,and the genotype frequency in the controls was 40.08％,52.60％,7.32％ and 35.81％,35.23％,28.96％ respectively,while the frequency in ARCs was 36.94％,56.25％,6.81％ and 36.67％,32.92％,30.42％,respectively.The genotype of another 2 SNPs in SIRT1 (rs2273773 and rs4746720) was both CC/CT/TT,and the genotype frequency in the controls was 38.37％,54.78％,5.85％ and 74.75％,17.69％,7.56％ respectively,while the frequency in ARCs was 39.58％,54.17％,6.25％ and 71.81％,20.97％,7.22％,respectively.And there was no significant difference between the two groups (P ＞ 0.05).Conclusion All the five SNPs of SIRT1 gene are not associated with ARC in the Jiangsu Han population.

AlM:To understand the effect on comprehensive ability of“Teaching-learning transaction” professional education of ophthalmic postgraduates which involving the teachers and students to participate in, and to explore new education model for ophthalmic postgraduate.METHODS:We carried out retrospective investigation questionnaire in the 31 ophthalmic postgraduates of Luzhou Medical College 2010 ~ 2014 grade and the 47 medical staffs of Department of Ophthalmology of the Affiliated Hospital of Luzhou Medical College, to clarify the new professional education model whether could improve the comprehensive ability of ophthalmic postgraduates or not. RESULTS: Carrying out the “ Teaching - learning transaction” professional education could improve the comprehensive ability of ophthalmic postgraduates.CONCLUSlON: Putting teaching and learning together can improve and promote each other, and it is helpful to cultivate comprehensive ability of ophthalmic postgraduates.

Objective To observe the effects of Bushen Yiqi recipe on aging rats′ exercise capacity, morphology and oxidation resis-tance.Methods Male sprague dawley rats were divided into normal group, model group, traditional Chinese medicine group, methandienone group.The rats in the latter three group were received a subcutaneous in-jection of 10%D-galactose with 0.1 mg? g -1? d-1 for 10 weeks.The rats in traditional Chinese medicine group and methandienone group were given medicine by gavage .The dosage is adults′7 times of per kilogram of body weight and equivalent dose respectively.The experiment were las-ted for 10 weeks.Then rats′exercise capacity were tested by treadmill. The Morphological structure of the skeletal muscle were observed by elec-tron microscope.Rats′advanced glycosylated end products( AGEs) were tested by Elisa method.Rats′superoxidedismutase ( SOD) and malondi-aldehyde ( MDA) content in blood serum were tested by spectrophotome-try.Results Compared with normal group, the rats′exercise capacity, the mitochondria number and volume of rats′skeletal muscle, the serum SOD activity, the thymus index and spleen index in model group dropped significantly.The serum AGEs and MDA content increased significant-ly.Compared with model group, the exercise capacity, the mitochondria number and volume of rats′skeletal muscle, the thymus index in traditional Chinese medicine group and methandienone group inceased obviously.The serum MDA content decreased obviously.Conclusion Bushen Yiqi recipe can slow the aging progress of skeletal muscle through improving the model rats′degenerative changes of the above-mentioned indexes.

Objective By detecting the distribution and homology analysis of Acinetobacter baumanii in the ICU ward,to con-trol nosocomial infection,provide theoretical basis to take effective measures.Methods From January 2016,20 patients in ICU of Shaanxi Provincial People's Hospital were taken into group.The samples of sputum and surrounding environment samples were cultured.The homology of Acinetobacter baumannii was analyzed by MALDI-Biotyper software,antimicrobial susceptibility test was analyzed by K-B.Results 27 strains of Acinetobacter baumannii were detected,mainly comes from sputum,hands of medical staffs and the environment,homology analysis results showed that the 27 strains of Acinetobacter baumannii was divided into two clusters(Ⅰtype 1 1 strains,Ⅱ type 1 6 strains),most of Acinetobacter baumannii were multi-resistant bacteria,except for the polymyxin B,minocycline and SCF.Conclusion The ways of transmission of Acineto-bacter baumanii in ICU were by medical personnel hand,pollution of the medical equipment and so on,strengthening the dis-infection and reasonable application of antimicrobial agents were taken advantageous for the prevention and control of acine-tobacter baumannii infection and transmission.

PURPOSE: The objectives of this study were to examine the difference between scores assigned by dementia patients and their caregivers to the patients' anxiety, depression, and quality of life. METHODS: After obtaining Institutional Review Board(IRB) approval, face-to-face interview with the patients and their caregivers respectively was conducted by trained graduate-level nursing students from December 2007 to February 2008. Patients' anxiety, depression, and quality of life were measured by patients and their caregivers. The data obtained were analyzed using the SPSS/WIN 14.0 program, which was used for frequency, percentage, mean , standard deviation, t-test, and Pearson's correlation. RESULTS: Significant relationships were reported between the depression rated by patients and that rated by their caregivers(r = .37, p = .019). In addition, there was no difference between the quality of life rated by patients and that rated by their caregivers(t = -7.11, p = .479). However, there was no significant relationship between the anxiety rated by patients and that rated by their caregivers(r = .21, p = .195). CONCLUSION: There were no differences on level of depression and quality of life of dementia patients measured by dementia patients and caregivers, However, dementia patients' anxiety level has discrepancy between them.
Anxiety ; Caregivers ; Dementia ; Depression ; Humans ; Quality of Life ; Students, Nursing

The theoretical basis, location, belonging of zang-fu, treatment function and indications, applying principle and manipulation of Umbilical Ring acupoints in Zhuang medicine are explained in this paper. According to Zhuang medicine, umbilicus is an epitome of the body and all the zang-fu and organs in the body have corresponding epitomes like a fetus in front-standing position. The umbilicus is not only a micro-diagnosis system, but also a window for illness treatment that could be divided into superficial, middle and deep layer to respectively communicate different zang-fu and organs. The umbilical inner ring and outer ring are collectively called Umbilical Ring acupoints, they could dredge paths, regulate the balance of qi and blood to regulate qi, expel poison, tonify deficiency and remove stasis to treat many types of diseases in the whole body.
Acupuncture Points ; Acupuncture Therapy ; Humans ; Medicine, Chinese Traditional ; Umbilicus ; anatomy & histology

OBJECTIVETo study the diagnosis of vasovagal syncope (VVS) in children with postural orthostatic tachycardia syndrome (POTS).

METHODSThe clinical data of 57 children with POTS diagnosed by the head-up tilt test between January 2007 and December 2010 were studied retrospectively. Of the 57 children, there were 29 boys and 28 girls who were aged from 5 to 16 years (12.2±1.9 years).

RESULTSTwenty-four (42%) out of 57 children were diagnosed with VVS by prolonging the duration of the head-up tilt test (for the children with orthostatic tolerance): cardioinhibitory (20 cases), mixed (3 cases) and vasoinhibitory (1 case). The POTS children with VVS were older than those without (13.0±1.4 years vs 11.5±2.1 years; P<0.05). There were no significant differences in gender and clinical symptoms between the POTS children with and without VVS.

CONCLUSIONSSome children with POTS may have coexisting VVS, suggesting that it is important to avoid misdiagnosis of VVS by prolonging the duration of head-up tilt test in POTS children with orthostatic tolerance. There are no obvious differences in gender and clinical symptoms between the POTS children with and without VVS.

Adolescent ; Child ; Child, Preschool ; Diagnostic Errors ; Female ; Humans ; Male ; Postural Orthostatic Tachycardia Syndrome ; complications ; Syncope, Vasovagal ; diagnosis ; Tilt-Table Test

OBJECTIVETo explore the myelo-protection effect of mdr1 transfected cord blood cells (CBMNCs) graft against high-dose homoharringtonine leukemia-bearing severe combined immunodeficient (SCID) mice model.

METHODSMultidrug resistant (mdr1)gene was transferred into CBMNCs by a retrovirus vector, containing full-length cDNA of human mdr1 gene. CBMNCs and high-titer retrovirus supernatant were cocultured with cytokine combinations for 5 - 6 days. The SCID mouse models bearing human HL-60 cell leukemia were divided into three groups. Group A received tail vein injection of 2 x 10(6) mdr1 gene transduced CBMNCs at day 1 and 3, groups B and C 2 x 10(6) un-transduced CBMNCs and same volume of normal saline, respectively. The 3 groups of the mouse model were treated with weekly escalated doses of homoharringtonine. The peripheral white blood cell (WBC) counts, the human leukemia cells percentage in peripheral blood, the histological findings of main organs were assayed. The CD33 positive HL-60 cells in bone marrow were determined by flow cytometry. The function and expression of mdr1 gene were examined by PCR, immunochemistry (IC) and DNR extrusion test in vivo.

RESULTS(1) mdr1 gene was transferred into CBMNCs successfully and the transfection frequency was 30%. (2) Leukemia SCID mice were xenotransplanted with mdr1-transfected BMMNCs by a programmed procedure and could be used as a valuable model for in vivo evaluating myelo-protection effects. (3) The transfected mice could tolerate homoharringtonine 5 approximately 6 folds higher than conventional dose and kept peripheral WBC count at a mean of 3 x 10(9)/L, with the peripheral human myeloid leukemia cells percentage decreasing to less than 5%. Histological examination showed that there was no leukemia infiltration in the main organs, the CD33 positive HL-60 cells in bone marrow were less than 5%. (4) The repopulation frequency of the transfected CBMNs in marrow were 9.13%. DNR extrusion test confirmed that the P-gp product maintained its biological function in the marrow.

CONCLUSIONmdr1 transferred-human CBMNC can xenotransplanted and repopulated in leukemia-bearing SCID mouse and are protected from chemotherapy-induced myelosuppression.

ATP-Binding Cassette, Sub-Family B, Member 1 ; genetics ; metabolism ; Animals ; Antineoplastic Agents, Phytogenic ; administration & dosage ; adverse effects ; therapeutic use ; Cord Blood Stem Cell Transplantation ; methods ; Female ; Fetal Blood ; cytology ; Genetic Vectors ; HL-60 Cells ; Harringtonines ; administration & dosage ; adverse effects ; therapeutic use ; Humans ; Leukemia, Promyelocytic, Acute ; drug therapy ; pathology ; surgery ; Leukocytes, Mononuclear ; cytology ; metabolism ; transplantation ; Male ; Mice ; Mice, SCID ; Random Allocation ; Retroviridae ; genetics ; Transfection ; Treatment Outcome ; Xenograft Model Antitumor Assays