1.Autoantibodies against the second extracellular loop ofβ1-adrenoceptor from patients with DCM in-hibit the proliferation of CD4+T lymphocytes byβ1-adrenoceptor
Jin WANG ; Li WANG ; Yunhui DU ; Hong YAO ; Huirong LIU
Chinese Journal of Microbiology and Immunology 2013;(7):518-524
Objective To investigate the effects of autoantibodies (β1-AA) against second extra-cellular loop of the β1-adrenergic receptor (β1-AR-ECⅡ) in sera of patients with dilated cardiomyopathy (DCM) on proliferation of rat CD4+T lymphocytes.Methods β1-AA in the sera of patients with DCM was purified by affinity chromatography .CD4+T lymphocytes were isolated by immunomagnetic microbeads from peripheral blood mononuclear cells of rats and its positive rate was detected by flow cytometry .CCK-8 meth-od was used to detect the proliferation of CD 4+T lymphocytes and flow cytometry was performed to measure the ratio of CD4+/CD8+T lymphocyte .Results The purity of isolated rat CD 4+T lymphocytes by immu-nomagnetic microbeads reached 97.7%.The proliferation of CD4+T lymphocytes stimulated by CD3/CD28 was inhibited by β1-AA in a concentration-dependent manner .However , IgG antibodies extracted from sera of healthy controls did not suppress lymphocyte proliferation (P>0.05).The suppression effect of β1-AA was inhibited after binding to antigenic peptides corresponding to β1-AR-ECⅡ and was completely blocked by metoprolol, a specific antagonist of β1-adrenergic receptor (β1-AR).In addition,β1-AA had no effects on the ratio of CD4+/CD8+T lymphocyte .Conclusion β1-AA isolated from DCM patients suppresses the pro-liferation of CD4+T lymphocytes through β1-AR pathway , which indicates that β1-AA can directly reduce the number of T lymphocytes and impair the function of T lymphocytes , resulting in immune system disorders and the development of DCM .
2.Cloning and Expression of ywtD Gene from B. subtilis NX-2 and the Enzymatic Degradation of ?-Polyglutamic Acid
Jing JIN ; Jun YAO ; Hong XU ; Lin XU ;
China Biotechnology 2006;0(05):-
The B.subtilis ywtD gene,encoding a ?-polyglutamic acid(?-PGA)depolymerase,was amplified from the genome of B.subtilis NX-2 by PCR.The comparability between the cloned ywtD gene sequence to the reported sequence is high to 99.0%.Only one of the substituted nucleotide base caused the change to the amino acid sequence.The recombinant plasmid pET-15b-ywtD was then transformed into E.coli Rosetta(DE3)and the ywtD gene product could be expressed with the induction of 0.5mmol/L IPTG.The YwtD protein exhibited a remarkable activity in ?-polyglutamic acid degradation.The molecular weight of ?-PGA could be reduced from 700kDa to 20kDa after 72h through the enzymatic hydrolysis and consequently trended to be constant.
3.Clinic study of myocardium protective effect of immunoinflammatory response induced by preinfarction angina
Xingyuan JIA ; Limei YAO ; Hong JIN ; Aiping WANG ; Qingmin WEI
Chinese Journal of Postgraduates of Medicine 2006;0(19):-
Objective To investigate the myocardium protective effect of immunoinflammatory response induced by preinfarction angina. Methods Eighty-eight patients of acute myocardial infarction were divided into preinfarction angina group (48 subjects) and sudden onset group (40 subjects). The incidence of severe arrhythmia,heart failure,cardiac shock and in-hospital mortality were assessed in the two groups. The myocardial infarction size,ventricular function,coronary angiography were compared between the two groups. Some immunoinflammatory markers levels were detected. Results In preinfarction angina group,the incidences of severe arrhythmia,heart failure,and in-hospital mortality were lower (P
4.Human Tumor Cells Apoptosis Induced by Dihydroartemisinin and Its Molecular Mechanism
Hong XIE ; Lijun CHEN ; Li YAO ; Qiuyue JIN ; Wenliang HU
China Pharmacy 2005;0(24):-
OBJECTIVE:To study the apoptosis of human leukemic cells induced by Dihydroartemisinin and its molecular mechanism.METHODS:Human leukemia K562 cells were treated by Dihydroartemisinin.The inhibitory effect on cell proliferation was assayed by MTT.Fluorescence microscopy was applied to observe the presence of apoptosis.The expression of caspase-3 was assayed with reverse transcription-polymerase chain reaction(RT-PCR).Levels of mitochondrial and cytoplasmic cytochrome C were determined using Western blot.RESULTS:After treatment with Dihydroartemisinin for 48 hours,the IC50 values of human leukemia K562 cells were 8? 10-5mol? L-1 detected at a wavelength of 570nm by MTT.Distinct morphology changes of cell apoptosis such as karyopyknosis and conglomeration were observed by Hoechst33342/PI staining.RT-PCR assay showed the expression of Caspase-3.Western-blot detection showed the decrease of mitochondrial cytochrome C concentration but the positive expression of cytoplasmic cytochrome C concentration.CONCLUSION:Dihydroartemisinin could inhibit proliferation and induce apoptosis of human leakemic K562 cells,this may partially attributed to the promotion of the delivery of cyt-c and the activation of caspase-3.
5.Applications of tree-shaped structure in DICOM based on XML.
Chinese Journal of Medical Instrumentation 2006;30(2):83-87
In order to improve the sharing of DICOM, a proposal is offered to introduce XML into DICOM on the SPIE 2005 and in this way, better readability and expansibility of DICOM files have been achieved. On this basis, the DICOM data structure is improved further by replacing the one-dimension linear data structure with multilayer tree-shaped data structure. And thus the nesting and dependence relationships between information object and data elements are much clearer and it is more convenient and easy to browse DICOM image files.
Database Management Systems
;
Information Storage and Retrieval
;
methods
;
standards
;
Medical Informatics
;
methods
;
Medical Records Systems, Computerized
;
Programming Languages
;
Radiology Information Systems
;
Software
;
User-Computer Interface
6.Clinical observation on treatment of albuminuria in patients with pregnancy-induced hypertension syndrome in puerperium by Xiaobai Decoction.
Jin-ping JIA ; Yao-xiang WU ; Guo-hong XIE
Chinese Journal of Integrated Traditional and Western Medicine 2007;27(7):644-646
OBJECTIVETo observe the effects of Xiaobai Decoction (XBD) in reducing albuminuria and shortening the duration of albuminuria in patients with pregnancy-induced hypertension syndrome (PIH) in puerperium.
METHODSEighty-five patients were given the conventional treatment with magnesium sulfate for relieving convulsion and lowering hypertension, at the same time, the treated group was given XBD additionally with the modification according to the symptoms. The treatment course for both groups was 14 days. Routine test of midstream urine was performed every three days, and 24 h-urinary protein was measured every week.
RESULTSThe therapeutic effect on the 43 patients of the treated group was markedly effective in 11 (25.6 % ), effective in 26 (60.4%) and ineffective in 6 cases (14.0%), the total effective rate being 86.0%; while in the 42 patients of the control group, the corresponding numbers were 5 (11.9%), 21 (50.0%), 16 (38.1%) and 61.9%, respectively, the efficacy of the former was significantly better (P < 0.05).
CONCLUSIONXBD is a simple, safe and effective drug for reducing albuminuria and shortening the duration of albuminuria in puerperium of PIH patients.
Adult ; Albuminuria ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Hypertension, Pregnancy-Induced ; drug therapy ; Phytotherapy ; Postpartum Period ; Pregnancy ; Treatment Outcome ; Young Adult
9.Primary Cutaneous Nocardiosis Associated with Intramuscular Injection
Hai WEN ; Jianghan CHEN ; Hong XU ; Zhirong YAO ; Jianhua WU ; Yuchong CHEN ; Hongmei ZHU ; Jin ZHAO
Chinese Journal of Dermatology 1994;0(05):-
Objective To investigate and report a group of patients with cutaneous nocardiosis occurring in China.Methods The skin lesion of twenty-three patients were examined and the pathogen was identified.The possible cause of this disease was also investigated.Results All the twenty-three patients belon g to a same village in a province.Skin lesions on buttoc ks manifested as nodules and abscesses whereas some other lesions as ulcers and draining sinuses,occurring 2-10mo nths after intramuscular injection.The pathogens were identified as Nocardia asteroides.Conclusion Twenty-three patients of cutaneous nocardiosis were related to intramu scular injection.
10.Expression and Significance of Soluble Interleukin-2 Receptor and Tumor Necrosis Factor ? in Pediatric Malignant Solid Tumors
hong, WANG ; jin-yao, QIN ; zhi-zhong, TAN ; chun-qiang, DONG
Journal of Applied Clinical Pediatrics 1986;0(01):-
Objective To explore the dynamic changes of soluble interleukin-2 receptor(SIL-2R) and tumor necrosis factor ?(TNF-?) levels in pediatric malignant solid tumors clinical value.Methods The levels of SIL-2R and tumor necrosis factor ?(TNF-?) were measured by ELISA in 15 cases with pediatric malignant solid tumors before and after chemotherapy.Results Before chemotherapy the levels of SIL-2R and TNF-? of every group patients were significantly higher those that of normal control group (P