Irradiation which acts directly and produces the reactive oxygen radicals by ionizing water molecules, causes significant morbidity and mortality. The muscle is damaged by direct action, oxygen radicals and the alterations of microcirculation and metabolism after irradiation. The changes of SOD immunoreactivities in muscles of the rats after irradiation were observed. The ultrastructural changes of the irradiated muscles with the pretreatment of SOD (superoxide dismutase) or without were also investigated. A total of 60 healthy Sprague-Dawley male rats weighing from 200g to 250g were used as experimental animals. Under urethane (1.15g/kg. IP.2 times) anesthesia,30 Gy irradiation to lower extremities by PICKER-C9 Cobalt-60 teletherapy unit was done. 15,000 unit/kg of SOD was administered intraperitoneally 1 hour before irradiation. The experimental animals were sacrificed 1 day, 3 days, 7 days, 2 weeks and 4 weeks after irradiation. The superficial portions of the mid-belly of the rectus femoris muscles were obtained and sliced into portions, 2 mm in length, 1 mm in width and in thickness. The specimens were prepared by routine methods for the electron microscopic observation. All preparations were stained with uranyl acetate and lead citrate and observed with a Hitachi-600 electron microscope. The other parts of mid-belly of the rictus femoris muscles were sectioned in 14 micrometer thickness with cryostat at -20 degrees C. The immunoreactivities of SOD by use of antihuman Cu, Zn-and Mn-SOD antibodies were observed. The results were obtained as follows . 1. After irradiation, the immunoreactivities of SOD in the rictus femoris muscle were decreased. 2 weeks after irradiation, the immunoreactivities of Cu, Zn-SOD were trace, which was lowest.4 weeks after irradiation, the immunoreactivities were trace or weak. 1 day after irradiation, the immunoreactivities of Mn-SOD were trace, which was lowest. The immunoreactivities of Mn-SOD were increased gradually 4 weeks after irradiation, the immunoreactivities of Mn- SOD were moderate or weak. 2. The ultrastructural changes in the rectus femoris muscles of the rats were getting severer and severer after irradiation. 2 weeks after irradiation, unclear A band and I band, myofibrillolysis, increased and dilated cistemae of sarcoplasmic reticulum and mitochondria with dilated cristae and electron lucent matrix were seen. 4 weeks after irradiation, lysis of sarcomere and increased cisternae of sarcoplasmic reticulum were seen. 3. The ultrastructural changes in the rectus femoris muscles of the rats were getting worse and worse after 3 days of irradiation with the pretreatment of SOD. 2 weeks after irradiation with the pretreatment of SOD, myofibrillolysis, increased and dilated cisternae of sarcoplasmic reticulum and damaged mitochondria were seen. 4 weeks after irradiation with the pretreatment of SOD, the ultrastructures of rectus femoris muscles were recovered to normal. Consequently, after irradiation of 30 Gy, the immunoreactivities of SOD are decreased and SOD attenuates the reversible changes of ultrastructures in muscles.
Animals ; Antibodies ; Citric Acid ; Humans ; Lower Extremity ; Male ; Metabolism ; Microcirculation ; Mitochondria ; Mortality ; Muscles ; Quadriceps Muscle* ; Rats* ; Rats, Sprague-Dawley ; Reactive Oxygen Species ; Sarcomeres ; Sarcoplasmic Reticulum ; Superoxide Dismutase ; Urethane

XO/XY mosaicism is frequently observed in the most patients with mixed gonadal dysgenesis. Mixed gonadal dysgenesis is a condition of Intersexuality characterized by asymmetric gonads or ambiguous genitalia or both. The external genitalia are always masculinized to some extent, on occasion represent a hypospadias as a common genetic form of a mixed gonadal dysgenesis. The disorder probably results from a cytogenic error that occurs early in embryogenesis. Phenotypic males with mixed gonadal dysgenesis have a high risk of developing gonadal tumors. We describe a case of hypospadias with a unilateral testis and XO/XY chromosome complement that is considered as a mixed gonadal dysgenesis.
Chromosome Aberrations* ; Complement System Proteins ; Disorders of Sex Development ; Embryonic Development ; Female ; Genitalia ; Gonadal Dysgenesis, Mixed ; Gonads ; Humans ; Hypospadias* ; Male ; Mosaicism ; Pregnancy ; Testis

PURPOSE: To determine the result of early surgery for intermittent exotropia for children under 4 year old and the factors which affect it. METHODS: The study subjects consisted of 25 children younger than 4 years who had received surgery for intermittent Exotropia. They were followed up for more than 1 year after surgery by the Department of Ophthalmology of the Gachon Medical School, between July 1998 and December 2003. The interval from onset to initial surgery, preoperative deviation angle, dissociated vertical deviation, stereopsis, preoperative patching, type of surgery, and initial postoperative deviation angle were compared and analyzed in a retrospective manner. RESULTS: The average age of the subjects and the preoperative deviation angle were 3.2 years and 28.67+/-8.08PD respectively. The average deviation angle at postoperative year 1 was 5.60+/-8.69PD. Out of the 25 patients, 19 (76%) showed a deviation angle less than 10PD were and 4 (16%) had recurrence of exotropia. Consecutive esotropia occurred in 2 (8%). The clinical factor statistically related to results was the initial postoperative deviation angle. The group who had esophoria of more than 10 PD (18.27+/-6.18 PD) showed 93% orthophoria at postoperative year 1, which was statistically significant. CONCLUSIONS: Though a patient be under 4 years old, if their deviation angle is in progress or if non-surgical treatment is ineffective in the case when surgery is delayed, it is presumed that performing surgery at postoperative day 1 with an overcorrection of about 10 PD is preferred.
Child* ; Child, Preschool ; Depth Perception ; Esotropia ; Exotropia* ; Humans ; Ophthalmology ; Recurrence ; Retrospective Studies ; Schools, Medical

A clinical observation was made on 52 cases of hypospadias which were treated at the department of Urology, Kyung Hee University Hospital during the period of January, 1972 through December, 1985 and the following results were obtained. 1. The patients` age were between 2 to 28 years. Only 22 patients(41.6%) were found in the age group under 6 years. 2. Of 52 cases of hypospadias, 27 cases(50.9%) were penoscrotal, 11 cases(22.7%) were penile, 6 cases(11.3%) were perineal, 4 case(7.5%) were scrotal, 3 cases were glandular and 1 case(1.9%) was chordee without hypospadias in type. 3. Cryptorchidism was the most common among the associated anomalies and it occupied 25% of all patients. 4. Of the 52 cases of hypospadias, one stage operation was done in 12 cases, two stage operation in 32 cases and their success rate was 58.5%, 40.6% in respectively. 5. The most common complication was fistula formation and it occurred in 20 cases(54.5%)of hypospadias repair.
Cryptorchidism ; Female ; Fistula ; Humans ; Hypospadias* ; Male ; Urology

PURPOSE: To examine the difference of clinical features according to age of onset of refractive accommodative esotropia. METHODS: We retrospectively analyzed the medical record of 52 cases of refractive accommodative esotropia for more than 12 months of follow-up from March, 2000 to December, 2003. The factors we compared were gender, age of first ocular examination, interval from age of onset to first glasses, preoperative refractive error, amount of deviation corrected with hyperopic glasses, stereopsis and associated ocular conditions. RESULTS: Cases were grouped according to age of onset. Fourteen (27%) patients were less than 2 years old., 32 (62%) were between 2 to 4 years old, and 6 (11%) were between 4 to 6 years old 6. the group less than 2 years old showed lower levels of preoperative refractive error than the group more than 2 years old. The younger the age of onset, the lager the deviation angle without hyperopic glasses. There were no differences according to age of onset in gender, interval from age of onset to first glasses, stereopsis, anisometropia or amblyopia. CONCLUSIONS: In refractive accommodative esotropia, the younger the age of onset is, the lager the deviation angle without hyperopic glasses and the greater the deviation can be corrected with hyperopic glasses.
Age of Onset* ; Amblyopia ; Anisometropia ; Child ; Child, Preschool ; Depth Perception ; Esotropia* ; Eyeglasses ; Follow-Up Studies ; Glass ; Humans ; Medical Records ; Refractive Errors ; Retrospective Studies

The purpose of this study was to compare and evaluate the differences in masticatory performance, muscle activity, and patterns of occlusal contact between persons with natural dentition and removable partial denture wearers. Twenty healthy adult subjects with more than 28 teeth and thirteen removable partial denture wearers that classified Kennedy classification I was selected. The degree of pulverized rice and peanut was measured and analyzed by means of sieving method to compare the masticatory performance. For the muscle activity, EMG was recorded in selected muscles(Temporalis and masseter muscle)during mastication and resting state. The occlusal record in maximal intercuspation was taken with a silicone occlusal bite registration material for analysis of the patterns of occlusal contact. The obtained results were as follows: When chewed peanuts, masticatory performance ratio at 10-sieve size was 81.31% in natural dentition group. In removable partial denture wearer, 27.01% without RPD and 69.09% with RPD. When chewed rice, 42.04%, 11.87%, and 21.58%, respectively. The differences of masticatory performance ratio between groups were statistically significant at the 0.05 level. The mean EMG value in resting state was 1.06mV on temporal muscle, 0.98mV on masseter muscle in natural dentition group. In removable partial denture wearers, 1.13mV on temporal muscle, 1.05mV on masseter muscle without RPD and 1.11mV on temporal muscle, 1.04mV on masseter muscle with RPD. The mean EMG value during mastication was 45.64mV in natural dentition group, and in removable partial denture wearers, 22.06mV without RPD and 31.01mV with RPD when chewed peanuts. When chewed rice, 45.24mV, 25.53mV and 32.14mV, respectively. The differences of mean masticatory EMG value between groups were statistically significant at the 0.05 level. The number of posterior occlusal contact point was 20.15 in natural dentition group and 11.92 in removable partial denture wearers. The area of perforated surface was 16.50mm2in natural dentition group and 6.06mm2in removable partial denture wearers. The area of contact surface was 78.93mm2, 51.52mm2, respectively. The area of contact surface was effective to masticatory performance ratio in natural dentition group and removable partial denture wearers (P<0.05) From these results, it is concluded that in partially edentulous patient, masticatory efficiency can be improved by removable partial denture wearing, and for effcient mastication, tigth occtact surface shoud be maintained by maximum support that is provided from mucosa.
Adult ; Arachis ; Classification ; Dentition ; Denture, Partial, Removable* ; Humans ; Jaw Relation Record ; Masseter Muscle ; Mastication ; Mucous Membrane ; Silicones ; Temporal Muscle ; Tooth

PURPOSE: Glyphosate is widely used and its toxic exposures are not rare. Occasionally, glyphosate intoxication can lead to death. The aim of this study is to analyze clinical findings and fatality in glyphosate intoxication. METHODS: Clinical data on acute glyphosate intoxication were prospectively collected at 28 hospitals nationwide between August 2005 and July 2006. The patients' clinical symptoms and characteristics of fatalities were investigated and statistical analysis was performed. RESULTS: Among 105 patients who were finally included, gastrointestinal symptoms(59%) were the most common. A significant difference in the amount ingested was observed between patients with higher systolic blood pressure and those with systolic blood pressure less than or equal to 80 mmHg (p<0.001). The more the patients ingested, the more aggravated their mental status became (p=0.004). Seven patients(6.7%) died, and all of them had ingested greater than or equal to 200 ml. Patients who died had ingested greater amounts than the survivors (p<0.001), and their mental status was worse (p<0.001), and systolic blood pressure was lower (p<0.001). According to the result of logistic regression analysis, relative risk was 24.1-fold higher in the 'poor' mental status group compared with 'good'. CONCLUSION: Patients who ingested large amounts of glyphosate showed poor mental status and lower blood pressure. Statistical difference in amount ingested, mental status, and systolic blood pressure was observed between survivors and patients who died. Ingested amounts and mental status were the most important factor of the prognosis of glyphosate intoxication.
Blood Pressure ; Herbicides ; Humans ; Logistic Models ; Poisoning ; Prognosis ; Prospective Studies ; Survivors

A balance between production and degradation of reactive oxygen species has an important role in the cardiovascular homeostasis, and is known to contribute to hypertension. Under oxidative stress, an upregulation of inducible NOS (iNOS) induces ischemic-reperfusion injury, and is involved in the pathophysiology of the hypertension. Ischemic-reperfusion injury of the skeletal muscle results from reactive oxygen species, and overexpression of iNOS in the skeletal muscle increases the ischemic injury. Superoxide dismutase (SOD), antioxidant, is a major enzyme for degradation of reactive oxygen species (ROS). The purpose of this study was to observe the effect ischemic preconditioning (IP) of the lower limb on the expression of iNOS, CuZnSOD and MnSOD in the white and red muscle of the spontaneously hypertensive rat (SHR). Nine weeks old male normotensive rat (Wistar-Kyoto rat, WKY) and SHR were divided into control and IP groups. The IP group was further divided into 3 (3IP) and 10 (10IP) times of IP. Left common iliac artery was occluded 3 and 10 times for 5 min of ischemia-5 min of reperfusion using rodent vascular clamp. The animals were sacrificed at 0, 0.5, 1, and 3 hours after reperfusion and the Tibialis anterior and Soleus were removed. The expressions of iNOS, CuZnSOD and MnSOD in the skeletal muscle were examined with immunohistochemical methods and Western blot analysis. iNOS was expressed in Tibialis anterior, but in Soleus after IP. The expression of iNOS was increased in both WKY and SHR, it was higher in SHR than WKY. CuZnSOD and MnSOD were expressed in Tibialis anterior and Soleus, higher in Soleus, after IP. The expression of CuZnSOD and MnSOD were increased in both WKY and SHR, higher in WKY than SHR. It is consequently suggested that hypertensive individual and white muscle are more sensitive to ischemic injury of the skeletal muscle as considering their high expression of iNOS and low expression of SODs.
Animals ; Blotting, Western ; European Continental Ancestry Group ; Homeostasis ; Humans ; Hypertension ; Iliac Artery ; Ischemic Preconditioning ; Lower Extremity ; Male ; Muscle, Skeletal ; Muscles ; Oxidative Stress ; Rats ; Rats, Inbred SHR ; Reactive Oxygen Species ; Reperfusion ; Rodentia ; Superoxide Dismutase ; Superoxides ; Up-Regulation

PURPOSE: Peptide YY is composed of 36 amino acids, and its functions are suppression of gastric acid secretion, delay of gastric emptying, increase of intestinal motility, inhibition of pancreatic exocrine secretion, and enhanced postprandial colonic absorption of water and electrolyte. PYY is released from PYY cells, which are mainly distributed in the ileum and colon, in response to the presence of intraluminal lipids. This study was designed to determine the regional distribution of PYY in the normal human ileum, colon, rectum, and anal canal by studying mucosal concentrations. METHODS: Fresh tissues were obtained from specimens of segmental resections of the small bowel, colectomies, ileostomies, and abdominoperineal resections for the management of colonic or rectal carcinomas or benign diseases of the bowel. Only specimens devoid of advanced luminal obstruction were included. Mucosa was separately recruited by microdissection of frozen sections. Radioimmunoassays were performed using the methods of Adrian et al. RESULTS: The concentration of PYY was highest at 25 cm proximal to the ileocecal valve in the ileum (307.6 pmol/ g) and in the upper rectum at the colon (653.1 pmol/g). The concentration of PYY was 27.3 pmol/g in the anal canal distal to the dentate line. A clear differential distribution of PYY was shown in the ileum and colon. CONCLUSIONS: This study showed PYY was present in large amounts in the mucosa of the ileum and colon, with high concentrations in locations 25 cm proximal to ileocecal valve and in the upper rectum, respectively. The trend of regional differences in PYY in the colonic mucosa probably reflects local differences in functions, such as absorption and storage. Also, the peak concentration in the ileum at 25 cm proximal to ileocecel valve suggests that this region is the most abundant production site of PYY in the ileum.
Absorption ; Amino Acids ; Anal Canal ; Colectomy ; Colon ; Frozen Sections ; Gastric Acid ; Gastric Emptying ; Gastrointestinal Motility ; Humans* ; Ileocecal Valve ; Ileostomy ; Ileum ; Microdissection ; Mucous Membrane* ; Peptide YY* ; Phenobarbital ; Radioimmunoassay ; Rectum

Pancreatic panniculitis is a rare disease in which necrosis of fat in the panniculus and other distant foci occur in the setting of pancreatic disease. The most common pancreatic disorders associated with pancreatic panniculitis are acute and chronic pancreatitis, especially the alcohol-related types and pancreatic carcinoma. We now report a case of pancreatic panniculitis in which skin eruptions, not abdominal symptoms, were the presenting symptom. A 45-year-old man presented with multiple erythematous tender subcutaneous nodules on the shins, arthralgia of both ankles and swelling of the left foot. He was diagnosed as having pancreatitis 15 months ago. Histopathologic findings of a skin biopsy showed focal fat necrosis with anucleated "ghost-like" fat cells with a thick shadowy wall. Laboratory evaluation revealed an increase in serum amylase and lipase. We diagnosed this patient's problem as pancreatic panniculitis associated with a relapse of pancreatitis.
Adipocytes ; Amylases ; Animals ; Ankle ; Arthralgia ; Biopsy ; Fat Necrosis ; Foot ; Humans ; Lipase ; Middle Aged ; Necrosis ; Pancreas ; Pancreatic Diseases ; Pancreatic Neoplasms ; Pancreatitis ; Pancreatitis, Chronic ; Panniculitis ; Rare Diseases ; Recurrence ; Skin