1.Advance in prevention and treatment of ischemia cardio-cerebrovascular disease through increased therapeutic angiogenesis induced by traditional Chinese medicine.
Hao GUO ; Lei LI ; Jin-Cai HOU ; Jian-Xun LIU
China Journal of Chinese Materia Medica 2015;40(1):24-28
Remaining organic and functional damage of ischemia cardio-cerebrovascular disease is always a main trouble puzzling the clinicians. After the discovery of endothelial progenitor cells (EPCs), researchers realize that postnatal angiogenesis is an important biological process, which play a key role to repair the ischemia tissue and improve the function. So a new concept which names therapeutic angiogenesis supply a new treament way for the ischemia cardio-cerebrovascular disease. Traditional Chinese medicine (TCM) has accumulated rich experience on treating the ischemia disease, studies found that many Chinese medicine prescriptions and effective ingredients can increase the therapeutic angiogenesis, howerer the mechanisms were not the same, they mainly manifest in regular the secretion of angiogenic factors, increase the proliferation and differentiation etc. In this paper, we review recent studies, summary the Chinese medicine prescriptions and effective ingredients which can increase the therapeutic angiogenesis, and analyze the differernt pathway. We view to provide reference for the later researchers.
Angiogenesis Inducing Agents
;
administration & dosage
;
Animals
;
Brain Ischemia
;
drug therapy
;
physiopathology
;
prevention & control
;
Cerebrovascular Disorders
;
drug therapy
;
physiopathology
;
prevention & control
;
Drugs, Chinese Herbal
;
administration & dosage
;
Humans
2.Value of three-dimensional power Doppler imaging for discrimination between benign and malignant endometrium in women with postmenopausal bleeding
Jin CHEN ; Lidan HAO ; Ruiqiang GUO ; Hua SHI ; Jiaqi HU
Chinese Journal of Ultrasonography 2011;20(4):321-323
Objective To evaluate the value of three-dimensional power Doppler imaging(3D-PDI) in the discrimination between benign and malignant endometrium in women with postmenopausal bleeding. Methods Eighty-four women with post menopausal bleeding who examined by 3D-PDI were enrolled. All patients scheduled for pathological examination were divided into benign group (30 cases with endometrial polyps and 34 cases with hyperplasia) and malignant group (20 cases with endometrial carcinoma). Endometrial volume, vascularity index ( VI) , flow index ( FI) and vascularity flow index ( VFI) were measured. The diagnostic value of parameters derived from 3D-PDI was evaluated by using the receiver operating characteristic ( ROC) curve. Results Differences of these parameters (VI, FI and VFI) were considered statistically significant between two groups, the parameters of endometrial volume had no significant differences between two groups; the area under ROC curve(AUC) of the meaningful data were 0.976,0.888 and 0.894 respectively. Among these parameters, VI had the highest diagnostic value for discriminating between benign and malignant endometrium, the cut-off value of which was 2. 97 with a sensitivity of 95% and a specificity of 86%. Conclusions 3D-PDI is a good diagnostic tools in the discriminations between benign and malignant endometrium in women with postmenopausal bleeding.
3.Effects of Ang-Ⅱ on c-fos mRNA and pro-α1ⅠmRNA of cadiac FBC in rat embryo
Yi, JIN ; Bao-yu, LI ; Cheng-hao, GUO
Chinese Journal of Endemiology 2001;20(3):169-171
Objective To observe the effects of Ang-Ⅱ on expressions of c-fos mRNA and pro-α1[Ⅰ]mRNA in fibroblasts (FBC) of rat embryo in the course of collagen synthesis.Methods The change of free Ca2+([Ca2+]i) was tested in fluorometry and the expressions of c-fos mRNA and pro-α1[Ⅰ]mRNA were tested by RT-PCR method in incubated FBC.Results The level of[Ca2+]i in FBC and the expressions of c-fos mRNA and pro-α1[Ⅰ]mRNA were enhanced with the increase of Ang-Ⅱ concentration(10-8~10-5and 10-7~10-6mol/L).Conclusions The expressions of c-fos mRNA and pro-α1[Ⅰ]mRNA were induced by Ang-Ⅱ activating signal system in FBC.
4.Relationship between endometrial blood flow measured by three-dimensional power Doppler ultrasound and pregnancy for women who received the treatment of in vitro fertilization and embryo transfer
Jin CHEN ; Lidan HAO ; Hua SHI ; Ruiqiang GUO
Chinese Journal of Ultrasonography 2011;20(10):861-863
ObjectiveTo evaluate whether endometrial blood flow measured by three-dimensional power Doppler ultrasound can predict pregnacy after the treatment of in vitro fertilization and embryo transfer (IVF-ET).MethodsNinty women who received the treatment of IVF-ET examined by threedimensional power Doppler imaging at the day of inject human chorionic gonadotropin(HCG) were enrolled.All patients were divided into pregnancy group (43 cases,include biochemical pregnancy and clinical pregnancy ) and non-pregnancy group(47 cases).The diagnostic value of parameters derived from threedimensional power Doppler imaging was evaluated by using the receiver operating characteristic (ROC)curve.ResultsDifferences of these parameters,including vascularization index (VI),flow index(Fl) and vascularization flow index (VFI)were considered statistically significant between two groups,the parameters of uterine artery and endometrial volume had no significant differences between two groups.The area under ROC curve of VI,FI and VFI were 0.880,0.713 and 0.646,respectively.Among these parameters,VI had the highest diagnostic value for discriminating between benign and malignant endometrium,the cut-off value of which was 0.733 with a sensitivity of 84.2%.Conclusions Threedimensional power Doppler ultrasound was useful for evaluating endometrial blood flow in IVF-ET cycles.Endometrial blood flow parameters may be useful predictors for pregnancy.
5.Expression of Dishevelled protein is correlated with the proliferation and invasion of glioma
Jian LI ; Geng GUO ; Yimin FAN ; Jiehe HAO ; Jin LI
Cancer Research and Clinic 2014;26(4):250-252,256
Objective To investigate the correlation between Dishevelled protein expression and the proliferation and invasion of glioma cells.Methods 67 cases of brain glioma specimens were collected.The expression of Dishevelled protein was detected with immunohistochemical method.The immunoreactivity score (IRS) of Dishevelled protein,and proliferation index (PⅠ) and invasion index (Ⅱ) were measured and their correlations were analyzed.Results The positive rate of Dishevelled protein in glioma was 65.7 % (44/67).IRS,PⅠ and Ⅱ were 4.15±3.13,(30.93±17.92) %,(20.38±13.36) %,respectively.Both PⅠ and Ⅱ significantly increased with an increase in the pathological grade of brain glioma (P < 0.001).Furthermore,PⅠ and Ⅱ were significantly higher in the Dishevelled protein-positive group than those in the Dishevelled protein-negative group [(38.27±17.60) % vs (16.02±8.92) % of PⅠ and (30.03±13.81) % vs (10.63±4.41) % of Ⅱ,respectively,P < 0.001].PⅠ and Ⅱ of glioma cells were positively correlated with IRS of Dishevelled protein (r =0.940 between PⅠ and IRS,and r =0.953 between Ⅱ and IRS,respectively).Conclusion Dishevelled protein plays an important role in the proliferation and invasion of brain malignant glioma.
6.Application of pancreatic duct guide wire and transpancreatic septotomy with precutting techniques in dififcult endoscopic retrograde cholangiopancreatography
Shangbo JIN ; Yimin LIU ; Jidong HE ; Zhihua GUO ; Hao SUN
China Journal of Endoscopy 2016;22(12):75-78
Objective To investigate the application value of pancreatic duct guide wire and transpancreatic septotomy with precutting technique in difficult endoscopic retrograde cholangiopancreatography.Method258 patients who underwent difficult endoscopic retrograde cholangiopancreatography from April 2014 to April 2016 were selected as study subject, 128 patients among them received the technique of pancreatic duct guide wire, the other 130 patients received transpancreatic septotomy with precutting techniques. The success rate, intubation time and incidence of complications were compared between these two methods for cannulation.Result There was no signiifcant difference in preoperative clinical data between the two groups, the success rate did not differ signiifcantly between the two groups (93.75 % vs 93.85 %). Compared with transpancreatic septotomy with precutting techniques group, pancreatic duct guide wire group is less intubation time consuming (5.92 ± 0.69 vs 12.81 ± 3.67) min, the difference was statistically significant (t = -2.27,P < 0.05). 25 patients experienced complications, with 6 cases of acute pancreatitis, 2 cases of biliray tract infection in pancreatic duct guide wire group, and 8 cases of acute pancreatitis, 3 cases of hemorrhage,6 cases of biliray tract infection in transpancreatic septotomy with precutting techniques group. The pancreatic duct guide wire group had a signiifcantly lower incidence of complications (6.25 % vs 13.08 %). the difference was statistically signiifcant (χ2 = 3.27,P < 0.05). The incidence of acute pancreatitis did not differ signiifcantly between the two groups (4.69 % vs 6.15 %).ConclusionsPancreatic duct guide wire and transpancreatic septotomy with precutting techniques both can further improve the success rate of bile duct cannulation with ERCP. The incidence of acute pancreatitis did not differ significantly between two groups. But pancreatic duct guide wire group is less intubation time consuming, and had a significantly lower incidence of complications. Because of the convenience and safety of the pancreatic duct guide wire technique, and the insertion of the pancreatic duct does not increase the risk of postoperative acute pancreatitis. We think that this method is more worthy of Clinical promotion.
7.THE EFFECT OF PRESERVATION MYCELIAL FUNGUS BY THE METHODS OF SLANT AND RUBBER PLUG
Shi-Gui LI ; Jin-Gang GU ; Hao-Li GUO ;
Microbiology 1992;0(04):-
This paper has reported the effect of preservation myceh'al fungus by the methods of slant and rubber plug. The mycelial fungus preserved include 29 genera, 69 species, 128 strains. The preservation time is from 2 years to 17 years. By slant inoculation, their survival conditions are still good keep their original charaters. The result shows that it is simple and effective preserving some mycelial fungus by the methods of slant and rubber plug.
8.Different metabolites of leaves between Tripterygium wilfordii and Tripterygium hypoglaucum based on UPLC-Q-TOF-MS.
Chao LIU ; Qing-xiu HAO ; Yan JIN ; Lu-qi HUANG ; Li-ping KANG ; Lan-ping GUO
China Journal of Chinese Materia Medica 2015;40(9):1710-1717
To analysis the differences between Tripterygium wilfordii and T. hypoglaucum, specimens of their leaves were collected from five production regions and analyzed by ultra performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS). The data were analyzed by multivariate statistical method, such as hierarchical cluster analysis (HCA) principal component analysis (PCA) and orthogonal signal correction partial least square discrimination (OPLS-DA). Potential markers with VIP values above 5.0 and corresponding r values above 0.85, were selected and further tested by combining mann-Whitney nonparametric. Those with P < 0.001 and AUC = 1 were confirmed as metabolite markers to discriminate them from each other. Results revealed that the two species were obviously different in their leaf metabolites. Based on their mass spectra, 23 potential metabolite markers were identified to distinguish T. wilfordii from T. hypoglaucum.
Chromatography, High Pressure Liquid
;
Drugs, Chinese Herbal
;
chemistry
;
metabolism
;
Mass Spectrometry
;
Molecular Structure
;
Plant Leaves
;
chemistry
;
metabolism
;
Tripterygium
;
chemistry
;
classification
;
metabolism
9.Government accountability in building trust between physicians and patients from the perspective of health-care service integration
Rui GUO ; Zhaofeng LYU ; Xiaoyan WANG ; Taoxin MO ; Jin HAO ; Yifan LI ; Jingnan MIAO ; Yang LIU
Chinese Journal of Hospital Administration 2015;(9):675-677
The study found that the physician-patient trust crisis results from overreliance on technology trust instead of interpersonal trust and institutional trust. The alleged “Paternalistic government innovation”in healthcare service has caused wastes of healthcare resources and gap below public expectancy due to its incompetence in resolving social problems,further eroding institutional legality and intensifying such crisis.This research aimed to identify government accountabilities in building such trust from three aspects.
10.Comparison of the effect of intervention embolization and operation in the treatment of cerebral aneurysms
Qiang GUO ; Feng JIN ; Deqin CHEN ; Shiqing YAN ; Lingsheng KONG ; Hao ZHANG ; Guangkui HAN
Chinese Journal of Primary Medicine and Pharmacy 2012;19(18):2725-2727
Objective To compare of intervention embolization and operation in the treatment of brain aneurysms.Methods 60 cerebral aneurysm patients using random number table method were divided into two groups each 30 cases,group A given intervention embolization;group B given surgical operation treatment.They were followed for 3months,the bleed Glasgow results score,matrix metalloproteinases 2(MMP-2) level change and the length of time were recorded.Results After treatment,A group total effective rate was 73.4%,postoperative treatment to bleed again rate was 10.0% (3/30),B group was 76.7%,6.7% (2/30),two groups had no statistically significant differences (x2 =0.09,0.22,all P > 0.05 ) before treatment M MP-2 level between ;The two groups was statistically significant ( t =1.21,P > 0.05 ) ;Two groups after surgical treatment 3 d MMP-2 levels were ( 20.1 ± 8.7 ) μg/L,( 35.7 ±8.9 ) μg/L respectively,which was statistically significant ( t =3.33,P < 0.05 ).A group of hospitalization time (8.6 ±1.5 ) d,group B ( 13.2 ± 1.3 ) d,the difference between two groups was statistically significant ( t =3.18,P < 0.05 ).Conclusion For brain aneurysm patients,the intervention embolization had no obvious difference in curative effect with operation,but could reduce the time in hospital and significantly reduced MMP-2 level.